СИНТЕЗ ТА ВЛАСТИВОСТІ НОВИХ НАНОСИСТЕМ АРҐЕНТУМУ
AgEDTA complexonate was synthesized, the manner of coordination of the Argentum (I) ion to the functional groups of complexone was determined. It was found that the solid complex precipitates from the solution in the form of a fine powder. It is shown that the size and shape of nanoparticles of Arge...
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| author | Berezhnytska, Оleksandra Viktoriia, Semeniv Karyna, Sikorska Kamenska , Tetyana Khrokalo, Ludmyla Trunova, Olena |
| author_facet | Berezhnytska, Оleksandra Viktoriia, Semeniv Karyna, Sikorska Kamenska , Tetyana Khrokalo, Ludmyla Trunova, Olena |
| author_institution_txt_mv | [
{
"author": "Оleksandra Berezhnytska",
"institution": "V.I.Vernadsky Institute of General and Inorganic Chemistry of NAS of Ukraine, Akad.Palladin Avenue, 32\/34, Kyiv, Ukraine, 03142"
},
{
"author": "Semeniv Viktoriia",
"institution": "National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute», 37, Prosp.Peremohy, Kyiv, Ukraine, 03056"
},
{
"author": "Sikorska Karyna",
"institution": "National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute», 37, Prosp.Peremohy, Kyiv, Ukraine, 03056"
},
{
"author": "Tetyana Kamenska ",
"institution": "National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute», 37, Prosp.Peremohy, Kyiv, Ukraine, 03056"
},
{
"author": "Ludmyla Khrokalo",
"institution": "National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute», 37, Prosp.Peremohy, Kyiv, Ukraine, 03056"
},
{
"author": "Olena Trunova",
"institution": "V.I.Vernadsky Institute of General and Inorganic Chemistry of NAS of Ukraine, Akad.Palladin Avenue, 32\/34, Kyiv, Ukraine, 03142"
}
] |
| author_sort | Berezhnytska, Оleksandra |
| baseUrl_str | https://ucj.org.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T08:23:45Z |
| description | AgEDTA complexonate was synthesized, the manner of coordination of the Argentum (I) ion to the functional groups of complexone was determined. It was found that the solid complex precipitates from the solution in the form of a fine powder. It is shown that the size and shape of nanoparticles of Argentum complexonate depend on the solvent-precipitator. A stable dispersed system based on the synthesized complexonate was obtained. The shape and position of the surface plasmon resonance bands confirm the presence of spherical nanoparticles with a size of 15–30 nm in both dispersed systems. Investigations of the biological activity of the powder of AgEDTA has shown that it has a high bactericidal effect against gram-positive bacteria. |
| doi_str_mv | 10.33609/2708-129X.87.02.2021.95-106 |
| first_indexed | 2025-09-24T17:43:37Z |
| format | Article |
| fulltext |
95
UDC 546.57, 544.77 doi: 10.33609/2708-129X.87.02.2021.95-106
SYNTHESIS AND PROPERTIES OF NEW NANOSYSTEMS
OF ARGENTUM
О. S. Berezhnytska 1,2*, V. S. Semeniv 1,2, K. A. Sikorska 2, Т. А. Kamenska 2,
L. A. Khrokalo 2, О. K. Trunova1
1 V. I. Vernadsky Institute of General and Inorganic Chemistry of the Ukrainian NAS, prospekt Pal
ladina 32/34, 03142 Kyiv, Ukraine
2 National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute», prospekt Pe
remohy 37, 03056 Kyiv, Ukraine
*е-mail: olekberez@gmail.com
AgEDTA complexonate was synthesized, the manner of coordination of the Argentum
(I) ion to the functional groups of complexone was determined. It was found that the solid
complex precipitates from the solution in the form of a fine powder. It is shown that the size
and shape of nanoparticles of Argentum complexonate depend on the solvent-precipitator.
A stable dispersed system based on the synthesized complexonate was obtained. The shape
and position of the surface plasmon resonance bands confirm the presence of spherical nan-
oparticles with a size of 15–30 nm in both dispersed systems. Investigations of the biological
activity of the powder of AgEDTA has shown that it has a high bactericidal effect against
gram-positive bacteria.
Keywords: nanosystem, Argentum, EDTA, complexonate, bactericidal effect.
INTRODUCTION. The unquenchable in-
terest in nanomaterials and nanosystems is
due to their unique properties and the possi-
bility of targeted synthesis of such materials,
in particular nanoparticles with required size
and predicted properties [1]. Increasing the
resistance of pathogenic microorganisms to
antibiotics and fungicides is the main problem
of medicine. It is well known that silver com-
pounds are active against 16 major species of
microorganisms without damaging the benefi-
cial microflora, which increases the interest of
scientists in their study [2, 3].
For many years in a row, the study of the
physicochemical and pharmacological proper-
ties of precious metals, in particular gold and
silver, has been topical. Whereas the use of gold
compounds is limited due to the ambiguity of its
properties, in particular toxicity [4–7], the anti-
microbial properties of silver are not in doubt.
To use nanoparticles in pharmaceutics, it is im-
portant to consider the composition of the start-
ing system to avoid additional toxic effects.
There are many publications on the synthe-
sis of silver nanoparticles, the study of their
physical, chemical, and biological properties.
PHISICAL CHEMISTRY SYNTHESIS AND PROPERTIES OF NEW NANOSYSTEMS OF ARGENTUM
96 ISSN 2708-129X. Укр. хім. журн., 2021
These characteristiсs depend on many factors,
such as the choice of the method of produc-
tion, stabilizer and reducing agent, the size and
morphological shape of the obtained particles
[8–10]. Despite the wide range of research re-
sults presented, obtaining stable silver-based
nanomaterials remains a challenge for re-
searchers.
Depending on the size, silver nanoparticles
can be used as catalysts, bactericidal coatings
for medical devices and water filters, drug de-
livery media, electronic components, and bi-
osensors, like preservatives and active ingre-
dients in cosmetics, light filters in optics, and
as antibacterial and therapeutic agents in me
dicine [11–15].
The silver nanoparticles can enter the hu-
man organism in a variety of ways, such as
through the skin, orally, by inhalation, and
injection, with different effects. Although the
toxicity of silver nanoparticles is also contro-
versial, the long-term use of AgNPs in various
industries proves its safety [16–18].
The researchers’ attention to the green syn-
thesis of AgNP is due to the effectiveness and
environmental friendliness of this method.
The reducing biological agent, which also acts
as a stabilizer, is the main requirement of this
synthesis. By selecting the plant extract, it is
possible to obtain nanoparticles of the desired
size. However, the disadvantages of this me
thod are that biological reagents can contain
many secondary compounds and metabolites,
temperature restrictions inherent in enzymes.
The consequence of thise is a low synthesis
yield [19, 20].
Evaporation-condensation and laser abla-
tion techniques are popular among the physical
methods. Both methods allow obtaining nano
particles of uniform size and high purity, be-
cause they do not require the use of additional
solvents, which can also emit toxic substances.
However, such a synthesis requires cumber-
some equipment and high energy costs. The
agglomeration of the obtained AgNPs is also
observed due to the absence of stabilizers in
these methods [21, 22].
The most developed and diverse are chemi
cal methods of obtaining silver nanoparticles,
among which electrochemical synthesis pre-
dominates, which allows one to control the size
of nanoparticles by regulating the parameters
of electrolysis; sonodecomposition using ultra
sonic waves; synthesis in aqueous foams as a
matrix; radiolysis; microwave synthesis, which
allows one to obtain stable systems at the high
concentration of AgNPs at the same tempera-
ture and exposure, and others that allow one
to obtain nanoparticles with a size in the range
of 5–90 nm. A common approach to synthe-
sis is the reduction of silver from its salts using
a variety of organic solvents. Thus, particles
are obtained, which are usually dispersed in
an aqueous medium and require stabilization
with surfactants [23, 24].
However, some methods do not require the
use of an additional stabilizer. These are the
Turkevich method or the citrate method, the
reduction of ionic silver with glucose, as well
as with the help of complexones. The use of
simple techniques that do not require addi-
tional complex equipment is the best way to
solve the problem of synthesis of nanoparticles
of required size both from a practical and an
economic point of view [25–28].
Complexones are polydentate ligands, or-
ganic chelating compounds consisting of ami-
no acid elements. These compounds are widely
used in medicine because they are a transport-
ing agent. Important among the complexones
UCJ № 2 / Vol. 87О. S. Berezhnytska, V. S. Semeniv, K. A. Sikorska, Т. А. Kamenska, L. A. Khrokalo , О. K. Trunova
97https://ucj.org.ua
are nitriloacetic acid (NTA), ethylenediamine-
tetraacetic acid (EDTA), and its structural iso-
mer ethylenediaminedisuccinic acid (EDDS).
The chelating agent increases the bioavaila-
bility of the metal, promotes its accumulation,
and increases biological activity. The main task
of this work is the synthesis of silver complex-
onates, their conversion to the disperse state,
and the synthesis of colloidal solutions by the
reduction of silver with complexones. This syn-
thesis does not require an additional stabilizer,
because the complexones have a diphilic struc-
ture, ie are surfactants. The complexes of d-me
tals based on complexones are characterized
by a good solubility in water, high stability, and
easy digestibility. The advantage of complex-
ones is that they are excreted from the organ-
ism in an unchanged state, which significant-
ly depends on the stability of the complexone
[29–31]. Besides, it was shown [32–35] that the
use of EDTA as a reducing agent in chemical
condensation allows one not only to stabilize
the system but also to control the particle size
depending on the solution pH. Thus, it is of
interest to determine correlations between the
properties of Argentum (I) complexonate and
disperse systems obtained by the reduction of
Argentum nitrate with Na2EDTA solution.
EXPERIMENT AND DISCUSSION OF
THE RESULTS
The synthesis was performed in aqueous
solutions at a concentration of the starting
components of 5 ∙ 10-2 M and a ratio of the
starting components of 1:2 and 2:1, metal salt
(AgNO3) and complexone (Na2EDTA), respec-
tively, pH=7–8. The choice of concentration is
due to the reduction processes that occur at
high concentrations, and pH of solutions. The
complexes were precipitated from the solution
with acetone or alcohol, filtered off and dried
in a vacuum desiccator over calcium chloride
(in a dark place). A white Na3[AgEDTA] pre-
cipitate separated from the solution during
precipitation and turned pink after filtration.
The dry precipitate has a dark brown color due
to the reduction of surface ions of silver.
The synthesis of disperse systems was per-
formed in aqueous solutions at a ratio of start-
ing components of 1:2, 1:3, 1:5, 2:1, 2:5 for
AgNO3 : Na2edta, respectively. Diluted solu-
tions with a concentration of starting the com-
ponents of 5∙10-4, 5∙10-3, 2.5∙10-4, 1∙10-3 M were
chosen for the study because the necessary
condition for obtaining highly disperse heter-
ogeneous systems is to carry out reactions in
dilute solutions, and an excess of one of the re-
agents. In the course of the study it was found
that the optimal concentration of solutions is
5∙10-3 M. A solution of Argentum nitrate was
gradually added to Na2EDTA solutions under
heating and with a magnetic stirrer, the pH
was adjusted with a 0.1M NaOH solution and
maintained at 8–8,5. The solutions were stirred
at 60оC for 60 minutes.
An IR spectrum was recarded to deter-
mine the manner of coordination of Argen-
tum to EDTA. The IR spectra were recorded
on a Specord M80 spectrometer in a range of
400–4000 cm-1 in tablets with KBr.
The absorption spectra of solutions were re-
corded on a Specord M40 spectrophotometer
in a range of 300–600 nm.
Distribution diagrams of the obtained na-
nosystems were investigated by the method
of dynamic light scattering on «ZetaSizerMal-
vern».
The powder micrographs were taken on a
Hitachi H–800 scanning electron microscope.
The antibacterial effect of the complex was
determined on a powder sample of AgEDTA.
PHISICAL CHEMISTRY SYNTHESIS AND PROPERTIES OF NEW NANOSYSTEMS OF ARGENTUM
98 ISSN 2708-129X. Укр. хім. журн., 2021
The test was performed by the disk-diffusion
method on a solid culture medium (agar).
Strains of Bacillus subtilis UKM B–5006T
(gram-negative spore-forming bacterium) and
Escherichia coli UKM B–906 (gram-negative
rod-shaped bacterium) were used as experi-
mental cultures. The inoculants were obtained
the day before the test on a liquid culture me-
dium, meat-peptone broth (BCH), followed by
dilution with a sterile physiological solutions.
The density of the inoculant was adjusted to 0.5
on the McFarland scale. The Petri dishes with
agar were inoculated with cultures and filter
paper disks moistened with distilled water and
an AgEDTA powder sample were spread over
the inoculated surface. The culture medium
was incubated at 37°C for three days. The size
of the zones of inhibition of bacterial growth
was measured with a caliper in mm.
Analysis of the IR spectra indicates that the
undissociated carboxyl group is not involved
in the complexation process. In the spectra of
the complex there is a shoulder at 1660 cm-1,
which corresponds to the valence vibrations of
uncoordinated (COOH) groups.
In the complexonate spectra, the bands
νas(COO-) and νs(COO-) in the region of 1310–
1400 cm-1 and 1500–1600 cm-1 are significant-
ly shifted to the low frequencies relative to the
free ligand, which indicates the formation of
bonds of metal ions to dissociated carboxyl
groups. The splitting of the band νsCOO- in-
dicates the non-equivalence of carboxyl group
binding and different covalent and donor-ac-
ceptor bonding mechanisms [36]. The diffe
rence in the position of the bands νs(COO-)
and νas(COO-) (Δν~200 см-1) indicates their
monodentate coordination to the metal ion.
Table 1 Assignment of frequencies in the IR spectrum of metal complexonates
ν(M-N) ν(M-O) ν
(CC) ν
(CN) ν s
COO- δ(СН2) ν as
COO- νCOOH νH2O
AgEDTA 480, 445,
390
540, 565,
582, 610
920,
875 1120 1400,
1340 1450пл
1582,
1640sh.
1660 sh.
3410,
3288sh.
Na2EDTA – – 920,
860 1095 1436 1460 1640 1670 3408
5∙10-3M aqueous solutions of complex-
onates were prepared for investigation of them
by the absorption spectroscopy method. Upon
dissolution, the complexonates form dark
orange solutions. The color of the solution is
due to the complexing central ion and the dis-
persion of the obtained systems. The presence
of a surface plasmon resonance (SPR) band in
the electron absortion spectra, which is a col-
lective oscillation of metal particles smaller
than the wavelength of electromagnetic radi-
ation, indicates the presence of nanoparticles.
From the shape and position of this band, we
can conclude about the dispersion, shape, and
size distribution of nanoparticles. In Fig. 1,
the maximum of the SPR band at ~ 410 nm
indicates a high dispersion of the system. The
particle size is in the range of 10–20 nm. The
position of the band indicates the sphericity of
the particles.
UCJ № 2 / Vol. 87О. S. Berezhnytska, V. S. Semeniv, K. A. Sikorska, Т. А. Kamenska, L. A. Khrokalo , О. K. Trunova
99https://ucj.org.ua
Figure 1 SPR bands of AgEDTA 1–3 hours,
2–21 day.
It is known that disperse systems are cha
racterized by both habituation and aging. To
analyze the aggregative stability of the systems,
they were studied for a long time. After 3 weeks,
the color of the solution became more saturated,
however, as can be seen from Fig.1. (curve 2),
the maximum of the band shifted by 5 nm to
the long-wavelength region, which indicates
the recrystallization of small particles with their
subsequent adsorption on larger particles. For
the most part, when a critical concentration of
micelle formation is reached, smaller spherical
particles are formed, which are thermodynami-
cally more stable and less prone to coagulation.
If the reduction processes continue in the sys-
tem, the concentration of particles will increase,
which, in turn, will promote coagulation and
cause the particles to enlarge, possibly with the
formation of ellipses or nanofibers [35, 37, 38].
The shape of the band has changed, and
it has become narrower, which indicates the
prevailing radius of particles of the same size
and the habituation of the disperse system.
The increase in intensity indicates an increase
in the number of nanoparticles in the system,
that is, about the «maturation» of the colloi-
dal solution. For the solutions prepared with
EDTA, the particle size was large, but the band
was much narrower, which indicates a small-
er distribution of nanoparticles by radius. For
cooling solutions of silver (I) disperse systems
obtained by reduction with EDTA, the elec-
tronic absorption spectra are shown in Fig. 2.
The shape and position of the surface plasmon
resonance (SPR) band substantially depend on
the concentration and time [38].
a b
Figure 2 SPR band for 0.005M solutions of the system (AgNO3): Na2EDTA after 7, 10 and 14 days (a)
micrograph of the system after 14 days, scale mark 100 nm
PHISICAL CHEMISTRY SYNTHESIS AND PROPERTIES OF NEW NANOSYSTEMS OF ARGENTUM
100 ISSN 2708-129X. Укр. хім. журн., 2021
With an increase in the complexone con-
centration in the system, the particle size and
polydispersity of the system increase, therefore,
to obtain stable dispersions, the concentration
of the initial components should not exceed
5 ∙ 10-3 M. This is due to the high reducing abi
lity of EDTA. The studies carried out showed
that the systems remained stable for a long
time, and with time the SPR band became
narrower, and the maximum became clearer
(Fig. 2).
Within 2 months from the moment of syn-
thesis, the shape and position of the SPR band
and the color of the solution did not change.
The position of the maximum and the width
of the SPR band of the complexonate solution
and the colloidal solution were different, which
indicates a different shape and size of nano-
particles. This may be due to different micelle
formulas and different charge of the colloidal
particle.
Schematically, the formation of silver nano
particles can be written as follows, based on
the high reducing properties of EDTA [39–41]:
Ag+ hν Ag0
For the same reasons, a problem arises
when writing the micelle formula. Based on
the classical approach, if silver is reduced to
form metal nanoparticles, the micelle formula
has the following form:
{mAg nAg+(n-x)NO3
-}х+x NO3
- .
In the case of a complexonate solution, we
can assume the reduction of surface molecules,
where a complex ion acts as a stabilizer, and
then a micelle can be depicted as follows:
{mAg nAgedta3-3(n-x)Na+}3х-3x Na+
An analysis of the distribution diagrams
(Fig. 3) shows the nanodispersity of the system
with the predominant radius of particles of the
same size.
a b
Figure 3 Diagrams to the distribution of particles behind the AgEDTA after 1 day (a), after 21 day (b).
We can state the uniform distribution of
the dispersed phase in the dispersion medium.
As can be seen from the diagram, at first small
particles prevail in the system; after 21 days,
the particle size in the system increases, which
is due to the recrystallization of small particles,
but the system is stable.
The particles absorbing in the range of
410–440 nm have a characteristic spherical
shape, which is confirmed by micrographs.
UCJ № 2 / Vol. 87О. S. Berezhnytska, V. S. Semeniv, K. A. Sikorska, Т. А. Kamenska, L. A. Khrokalo , О. K. Trunova
101https://ucj.org.ua
As can be seen from the micrograph of the
pre-dried solution (Fig. 4), even when the
sample dries, the nanoparticles do not ag-
glomerate, but retain their original size and
spherical symmetry. Small spherical particles
are attached to a fibrous surface, which can be
ethylenediaminetetraacetate (Fig. 5). This in-
dicates the stabilizing effect of EDTA.
a b c
Figure 4 Distribution diagram and micrograph for systems (AgNO3): Na2EDTA, c = 0.005М, scale
mark 100 nm (b) 500 nm (c).
It is known that the method of physical con-
densation, namely the replacement of the sol-
vent, allows the targeted control of the particle
size. Therefore, it was of interest to precipitate
complexonates with various solvents.
a b
Figure 5 SEM micrographs of the test solutions,
scale mark 100 nm (a) and 200 nm (b)
Already in the process of salting out, it was
noticeable that the powder particles will have
different shapes. So, when salting out from ace-
tone, the particles simply settled to the bottom,
like «classic» sediment or spherical particles,
which precipitate under the action of gravity
due to the violation of aggregative and sedi-
mentation stability. The process of precipita-
tion occurred fairly quickly. In the case of alco-
hol as a precipitant, the salting-out process was
similar to a «blizzard» and was significantly
extended in time.
Using the method of electron microscopy, it
was shown that, depending on the solvent with
which the complexes were precipitated, nan-
oparticles can have different shapes and sizes.
Figure 6a shows micrographs of an AgEDTA
powder obtained by precipitation from ace-
tone, and in Fig. 6b, by precipitation from etha-
nol. When precipitated with acetone, the nano
powder particles have a spherical shape with
layered surface morphology. The particle size
is in the range of 20-33 nm, which correlates
fairly well with previous studies. Salting out
the silver complexonate with alcohol makes
it possible to obtain elongated particles. The
PHISICAL CHEMISTRY SYNTHESIS AND PROPERTIES OF NEW NANOSYSTEMS OF ARGENTUM
102 ISSN 2708-129X. Укр. хім. журн., 2021
micrographs of this powder are significantly
different, since the particles are in the form of
nanowires, and the surface morphology is fi-
brous, like the particles. The difference in the
photomicrographs of the samples makes it
possible to envisage different properties of the
obtained complexonates.
a b
Figure 6 Photomicrographs of AgEDTA pow-
der sample obtained by alcohol salting (a) acetone
salting (b).
The antibacterial activity was studied using
the example of a system containing spherical
nanoparticles of AgEDTA, which was deter-
mined from the size of the formed inhibition
zone concerning two types of pathogenic bac-
teria: Bacillus subtilis and Escherichia coli. Af-
ter 72 hours of cultivation in an incubator at
37° C, zones appear in the form of bright stains
and transparent concentric circles around the
disks with powder (Fig. 7). This indicates the
effectiveness of the sample, in particular, in-
hibition of bacterial growth. In the culture
medium of E. coli, a moderately pronounced
zone of growth inhibition is observed, 0.5–3
mm in size (Fig. 7a). In the culture of B. sub
tiles, Fig. 7b, non-concentric zones, 2–10 mm
in size, are very well pronounced.
a b
Figure 7. Inhibition zone size, against two types
of pathogens: (a) E. coli UKM B-906, (b) B. subtilis
UKM B-5006T.
CONCLUSIONS
AgEDTA complexonate, a nanodisperse sys-
tem based on it, and a nanosilver solution have
been obtained by the reduction of Argentum (I)
nitrate with a Na2EDTA solution were obtained.
The particle size of the precipitated complex-
onate powder was in the range of 20–70 nm de-
pending on the solvent used in the precipitation
process. It has been found that the size and shape
of the particles depend on the nature of the sol-
vent precipitator. Studies of colloidal solutions
have shown that reduction using EDTA allows
one to obtain nanodisperse systems with a par-
ticle size of the dispersed phase of 10–30 nm.
Such systems are quite stable and do not re-
quire additional stabilization. We can assume
that due to the low stability constant of silver (I)
complexonate in both cases there are metal na-
noparticles in the system. Metal nanoparticles
are stabilized by the complex ion AgEDTA3-
in the case of complexonate, and in the case
of colloidal solutions, the stabilizer is a solu-
tion of Na2edta. The particle size of AgEDTA
powder is 20–50 nm when precipitated with ac-
etone, and the diameter of the alcohol-precip-
itated nanowires is 25–65 nm. The larger par-
ticle size of the powder compared to solutions
(10–30 nm) is due to its conglomeration.
UCJ № 2 / Vol. 87О. S. Berezhnytska, V. S. Semeniv, K. A. Sikorska, Т. А. Kamenska, L. A. Khrokalo , О. K. Trunova
103https://ucj.org.ua
Studies of biological activity have shown
that the AgEDTA complex has insignificant
antibacterial activity against gram-negative
bacteria but proved to be quite effective versus
gram-positive ones due to differences in the
composition and thickness of the outer mem-
brane of pathogens.
Thus, the studies show the effectiveness of
the method of complexation and reduction of
complexone to obtain stable highly disperse
systems of silver.
The work was performed within the
framework of the target program of
basic research of the National Acade-
my of Sciences of Ukraine "Prospective
basic research and innovative developments
of nanomaterials and nanotechnologies for
the needs of industry, health care and agri-
culture" 31/20-H
СИНТЕЗ ТА ВЛАСТИВОСТІ НОВИХ
НАНОСИСТЕМ АРҐЕНТУМУ
Бережницька О. С. 1,2, Семенів В. І. 1,2,
Сікорська К. А. 2, Каменська Т. А. 2,
Хрокало Л. А. 2, Трунова О. К.1
1 Інститут загальної та неорганічної хімії
ім. В. І. Вернадського НАН України, просп.
Академіка Палладіна, 32/34, Київ 03142,
Україна
2 Національний технічний університет Ук
раїни “Київський політехнічний інститут”
імені Ігоря Сікорського, просп. Перемоги, 37,
Київ 03056, Україна
е-mail: olekberez@gmail.com
Синтезовано комплексонат AgEDTA,
встановлено спосіб координації іону арґен
туму (І) з функціональними групами комп-
лексону. Різниця в положенні смуг валент-
них симетричних та асиметричних коли-
вань карбоксильної групи свідчить про
їхню монодентатну координацію до іону
металу. Встановлено, що твердий комплекс
осаджується з розчину у вигляді дрібно-
дисперсного порошку. Показано, що роз-
мір та форма наночастинок порошку комп-
лексонату аргентуму залежать від розчин-
ника-осаджувача.
Отримано стійкі наносистеми двома
шляхами: розчиненням твердого комплек-
сонату та відновленням срібла розчином
ЕДТА. Форма та положення смуг поверхне-
во плазмонного резонансу підтверджують
наявність в обох дисперсних системах сфе-
ричних наночастинок розміром 15–30 нм.
Звуження лінії та зростання інтенсивності
смуги ППР свідчить про звикання дисперс
них систем, а відсутність суттєвого зміщен-
ня максимуму смуги – про агрегативну та
седиментаційну стійкість колоїдних розчи-
нів. Положення максимуму та ширина сму-
ги ППР розчину комплексонату та коло-
їдного розчину відрізнялися, що свідчить
про різний розмір наночастинок. Методом
електронної мікроскопії встановлено фор-
му, дисперсність та морфологію отриманих
наносистем. Показано, що AgNPs одержа-
ні методом відновлення, з використанням
як відновлюваного агента розчину ЕДТА,
мають сферичну форму, а їхній розмір зна-
ходиться в діапазоні 10–30 нм. У випадку
комплексонату AgEDTA розмір частинок
порошку при осадженні ацетоном стано-
вить 20–50 нм, при осадженні спиртом –
діаметр отриманих нанодротів 25–65 нм.
PHISICAL CHEMISTRY SYNTHESIS AND PROPERTIES OF NEW NANOSYSTEMS OF ARGENTUM
104 ISSN 2708-129X. Укр. хім. журн., 2021
Діаграми розподілу дисперсних систем до-
бре корелюють з електронними мікрофото-
графіями як щодо розподілу частинок, так і
їхнього переважаючого радіусу.
Дослідження бактерицидної дії пока-
зали, що комплекс AgEDTA має незнач-
ну антибактеріальну активність проти
грамнегативної бактерії, є ефективним про-
ти грампозитивної, що зумовлено різницею
складу та товщиною зовнішньої мембрани
патогенів.
Ключові слова: наносистеми, срібло,
комплексонат, EDTA, бактерицидна дія.
REFERENCES
1. Lee S.H., Sung J.H. Park, T.H. Nanomate-
rial-Based Biosensor as an Emerging Tool
for Biomedical Applications. Annals of Bio
medical Engineering. 2012. 40: 1384–1397.
2. Zhao G., Stevens SE Jr. Multiple parame-
ters for the comprehensive evaluation of
the susceptibility of Escherichia coli to
the silver ion. Biometals : an Internation
al Journal on the Role of Metal Ions in Bio
logy, Biochemistry, and Medicine. 1998. 1:
27–32.
3. Prabhu, S., Poulose, E.K. Silver nanopar-
ticles: mechanism of antimicrobial ac-
tion, synthesis, medical applications, and
toxicity effects. International Nano Let
ters. 2012. 2: 32.
4. Pizent A, Tariba B, Živković T. Reproduc-
tive toxicity of metals in men. Archives of
Industrial Hygiene and Toxicology. 2012.
63(1): 35–46.
5. Xu, L., Liu, Y., Chen, Z., Li, W., Liu, Y.,
Wang, L., Liu, Y.,. Wu, X., Ji, Y., Zhao, Y.
Surface-engineered gold nanorods: promi
sing DNA vaccine adjuvant for HIV-1
treatment. Nano Letters. 2012. 12(4):
2003–2012.
6. Pekkanen AM., DeWitt MR., Rylan
der MN. Nanoparticle enhanced optical
imaging and phototherapy of cancer. Jour
nal of Biomedical Nanotechnology. 2014.
10(9): 1677–1712.
7. Sosenkova, L.S., Egorova, E.M. The Effect
of Particle Size on the Toxic Action of Sil-
ver Nanoparticles. Journal of Physics: Con
ference Series. 2011. 291.
8. Madeira JM, Gibson DL, Kean WF, Kle
geris A. The biological activity of aurano-
fin: implications for novel treatment of
diseases. Inflammopharmacology. 2012.
20(6): 297–306.
9. Lee SH, Jun BH. Silver Nanoparticles: Syn-
thesis and Application for Nanomedicine.
International journal of molecular sciences.
2019. 20(4): 865.
10. Iravani S., Korbekandi H., Mirmohamma-
di SV., Zolfaghari B. Synthesis of silver na-
noparticles: chemical, physical and biolo
gical methods. Res Pharm Sci. 2014. 9(6):
385–406.
11. Ramanathan S., Gopinath S.C.B. Poten-
tials in synthesizing nanostructured silver
particles. Microsystem Technologies. 2017.
23: 4345–4357.
12. Ortega F, Arce VB., Garcia MA. Nanocom-
posite starch-based films containing sil-
ver nanoparticles synthesized with lemon
juice as reducing and stabilizing agent.
Carbohydrate polymers. 2021. 252: 117208.
13. Jatoi A.W., Kim I.S., Ni QQ. A compara-
tive study on synthesis of AgNPs on cellu-
lose nanofibers by thermal treatment and
UCJ № 2 / Vol. 87О. S. Berezhnytska, V. S. Semeniv, K. A. Sikorska, Т. А. Kamenska, L. A. Khrokalo , О. K. Trunova
105https://ucj.org.ua
DMF for antibacterial activities. Materials
science & engineering. C, Materials for bio
logical applications. 2019. 98: 1179–1195.
14. M. Mehdi, M. Akhtar, S. Abro. Electro-
chemical synthesis of AgNP and mechani
cal performance of AgNP-EG coatings on
soft elastomer. Journal of Elastomers &
Plastics. 2020. 52(7): 609–619.
15. Kokura S, Handa O, Takagi T, Ishikawa T,
Naito Y, Yoshikawa T. Silver nanoparticles
as a safe preservative for use in cosmetics.
Nanomedicine. 2010. 6(4): 570–574.
16. Roy S., Shankar S., Rhim J. Melanin-me-
diated synthesis of silver nanoparticle and
its use for the preparation of carrageen-
an-based antibacterial films. Food Hydro
colloids. 2019. 88: 237–246.
17. Shamsutdinova I.R., Derkho M.A. Fea-
tures of biological action of silver nano
particles in animals. Proceedings of the
Orenburg State Agrarian University. 2016
1(57): 202–205.
18. Chekman I S., Priskoka A.O., Babiy V.F.,
Antonenko O.V., Zahorodniy M. I. Medi
cal use of silver nanoparticles: toxicologi-
cal Aspect. Modern problems of toxicology.
2010. 4: 10–13.
19. Stanishevskaya I.E., Stoinova A.M., Mara-
khova A.I., Stanishevskiy Y.M. silver nano
particles: preparation and use for medical
purposes. Drug development & registra
tion. 2016. 1: 66–69.
20. Srikar S.K., Giri D.D., Pal D.B., Mishra P.K.
and Upadhyay S.N. Green Synthesis of Sil-
ver Nanoparticles: A Review. Green and
Sustainable Chemistry. 2016. 6: 34–56.
21. Mousavi-Khattat M., Keyhanfar M., Raz-
mjou A. A comparative study of stability,
antioxidant, DNA cleavage and antibac-
terial activities of green and chemically
synthesized silver nanoparticles. Artificial
cells, nanomedicine, and biotechnology.
2018. 46(3): S1022–S1031.
22. Simakin A., Voronov V., Kirichenko N.,
Shafeev G. Nanoparticles produced by
laser ablation of solids in liquid environ-
ment. Applied Physics A. 2004. 79: 1127–
1132.
23. Abou El-Nour K.M.M., Eftaiha A., Al-
Warthan A., Ammar R.A.A. Synthesis and
applications of silver nanoparticles. Arabi
an Journal of Chemistry. 2010. 3: 135–140.
24. Wiley B., Sun Y., Mayers B., Xia Y.
Shape-controlled synthesis of metal na-
nostructures: the case of silver. Chemistry.
2005. 11(2): 454-63.
25. Landage S., Wasif A., Dhuppe P. Synthesis
of nanosilver using chemical reduction
methods. International Journal of Ad
vanced Research in Engineering and Ap
plied Sciences. 2014. 3: 14–22.
26. Bogacheva, N.V., Tarbeeva K.A., Ogoro-
dova N.Y. Development of a step-by-step
procedure for obtaining silver nanopar-
ticles by the citrate method. Proceedings
of higher educational institutions. Series
«chemistry and chemical technology». 2020.
63(5): 65–69.
27. Semenenko V.A., Nabiullin A.R., Petru
shenko L.G. Obtaining silver nanoparti-
cles and research of their properties. Inter
national Scientific and Practical Conference
of Students and Young Scientists. Actual
problems of modern medicine and pharma
cy. 2016. 70: 1122–1125.
28. Kolyada L., Medyanik N., Efimova Yu.,
Kremneva A. Synthesis and research of
silver nanoparticles and the possibili-
ty of their use in food packaging. Vestnik
MGTU im. G.I. Nosova. 2015. 2: 50.
PHISICAL CHEMISTRY SYNTHESIS AND PROPERTIES OF NEW NANOSYSTEMS OF ARGENTUM
106 ISSN 2708-129X. Укр. хім. журн., 2021
29. Bychkov A.L., Ryabchikova E.I., Koro
lev K.G., Bukhtoyarov V.A. Obtaining
nanosized silver particles stabilized by
hydrolysis products of yeast biopolymers.
Vestnik VGUIT. 2019. 1: 79.
30. Nikolsky V.M., Pchelkin P.E., Sharov S.V.,
Knyazeva N.E., Gorelov I.P. Synthesis and
application of complexones, derivatives of
succinic acid, in industry and agriculture.
Success of modern natural science. 2004. 2:
71.
31. Joanne S., Stephen D., David R. Chemical
speciation of ethylenediamine-N,N′-di-
succinic acid (EDDS) and its metal com-
plexes in solution. Chemical Speciation &
Bioavailability. 1999. 11: 3, 85–93.
32. Asemave K. Greener Chelators for Re-
covery of Metals and Other Applications.
Organic & Medicinal Chem IJ. 2018. 6(4):
555–694.
33. Schulz F., Homolka T., Bastús N.G., Pun
tes V., Weller H., Vossmeyer T. Little
adjustments significantly improve the
Turkevich synthesis of gold nanoparticles.
Langmuir : the ACS journal of surfaces and
colloids. 2014. 30(35): 10779–10784.
34. Fabrikanos, V. A., Athanassio, S., Lie
se, K. H. Darstellung Stabiler Hydrosole
von. Gold und Silber durch Reduktion mit
Äthylendiamintetraessigsaure. Z. Natur-
forschg. 1963, 18b: 612–23 617.
35. Dozol H., Mériguet G., Ancian B., Ca
buil V., Xu H., Wang D., Abou-Hassan A.
On the Synthesis of Au Nanoparticles Us-
ing EDTA as a Reducing Agent. The Jour
nal of Physical Chemistry C. 2013. 117(40):
20958–20966.
36. Dyatlova N.M., Temkina V.Ya., Popov K.I.,
Komplexones and complexonates. M.: Khi
miya. 1988: 544. (in russian)
37. Barna A.W., Lampeka Ya.D. Influence of
the chemical nature of polyoxometallate
complexes on the flow of redox processes
with the formation of nanoparticles of me-
tallic silver. Theoretical and experimental
chemistry. 2012. 48(4): 224–229.
38. Shevtsova V.I., Gaiduk P.I. Position of the
surface plasmon resonance band in collo
idal solutions of silver and gold nanoparti-
cles. Vestnik BSU. 2012. 1(2): 15–18.
39. Motekaitis, R. J. Martell, A. E., Hayes, D.,
Frenier, W. W. The Iron (Iii)-Catalyzed
Oxidation of Edta in Aqueous-Solution.
Canad. J. Chem. 1980. 58: 1999–2005.
40. Bose, R. N.; Keane, C.; Xidis, A.; Reed, J. W.;
Li, R. M.; Tu, H.; Hamlet, P. L. Oxidation
of Ethylenediaminetetraacetic Acid by
Permanganate Ion – a Kinetic-Study. In-
org. Chem. 1991. 30: 2638–2642.
41. Khan Z.; Raju, K.-U. D. Kinetics of Oxida-
tion of Ethylenediaminetetraacetic Acid
(EDTA) by Chromium (VI) in the Pres-
ence of Perchloric Acid. Indian J. Chem.
Sect. B. 2004. 43(16): 149–156.
Стаття надійшла 27.02.2021.
|
| id | oai:ojs2.1444248.nisspano.web.hosting-test.net:article-279 |
| institution | Ukrainian Chemistry Journal |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2026-07-23T01:05:58Z |
| publishDate | 2021 |
| publisher | V.I.Vernadsky Institute of General and Inorganic Chemistry |
| record_format | ojs |
| resource_txt_mv | ucjorgua/c2/7c3be8851237db42c7720407da11a2c2.pdf |
| spelling | oai:ojs2.1444248.nisspano.web.hosting-test.net:article-2792026-07-22T08:23:45Z SYNTHESIS AND PROPERTIES OF NEW NANOSYSTEMS OF ARGENTUM СИНТЕЗ ТА ВЛАСТИВОСТІ НОВИХ НАНОСИСТЕМ АРҐЕНТУМУ Berezhnytska, Оleksandra Viktoriia, Semeniv Karyna, Sikorska Kamenska , Tetyana Khrokalo, Ludmyla Trunova, Olena nanosystem, Argentum, EDTA, complexonate, bactericidal effect. AgEDTA complexonate was synthesized, the manner of coordination of the Argentum (I) ion to the functional groups of complexone was determined. It was found that the solid complex precipitates from the solution in the form of a fine powder. It is shown that the size and shape of nanoparticles of Argentum complexonate depend on the solvent-precipitator. A stable dispersed system based on the synthesized complexonate was obtained. The shape and position of the surface plasmon resonance bands confirm the presence of spherical nanoparticles with a size of 15–30 nm in both dispersed systems. Investigations of the biological activity of the powder of AgEDTA has shown that it has a high bactericidal effect against gram-positive bacteria. V.I.Vernadsky Institute of General and Inorganic Chemistry 2021-03-19 Article Article Physical chemistry Физическая xимия Фізична xімія application/pdf https://ucj.org.ua/index.php/journal/article/view/279 10.33609/2708-129X.87.02.2021.95-106 Ukrainian Chemistry Journal; Vol. 87 No. 2 (2021): Ukrainian Chemistry Journal; 95-106 Украинский химический журнал; ##issue.vol## 87 ##issue.no## 2 (2021): Украинский химический журнал; 95-106 Український хімічний журнал; Том 87 № 2 (2021): Український хімічний журнал; 95-106 2708-129X 2708-1281 en https://ucj.org.ua/index.php/journal/article/view/279/156 Copyright (c) 2021 Оleksandra Berezhnytska, Semeniv Viktoriia, Sikorska Karyna, Tetyana Kamenska , Ludmyla Khrokalo, Olena Trunova https://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | Berezhnytska, Оleksandra Viktoriia, Semeniv Karyna, Sikorska Kamenska , Tetyana Khrokalo, Ludmyla Trunova, Olena СИНТЕЗ ТА ВЛАСТИВОСТІ НОВИХ НАНОСИСТЕМ АРҐЕНТУМУ |
| title | СИНТЕЗ ТА ВЛАСТИВОСТІ НОВИХ НАНОСИСТЕМ АРҐЕНТУМУ |
| title_alt | SYNTHESIS AND PROPERTIES OF NEW NANOSYSTEMS OF ARGENTUM |
| title_full | СИНТЕЗ ТА ВЛАСТИВОСТІ НОВИХ НАНОСИСТЕМ АРҐЕНТУМУ |
| title_fullStr | СИНТЕЗ ТА ВЛАСТИВОСТІ НОВИХ НАНОСИСТЕМ АРҐЕНТУМУ |
| title_full_unstemmed | СИНТЕЗ ТА ВЛАСТИВОСТІ НОВИХ НАНОСИСТЕМ АРҐЕНТУМУ |
| title_short | СИНТЕЗ ТА ВЛАСТИВОСТІ НОВИХ НАНОСИСТЕМ АРҐЕНТУМУ |
| title_sort | синтез та властивості нових наносистем арґентуму |
| topic_facet | nanosystem Argentum EDTA complexonate bactericidal effect. |
| url | https://ucj.org.ua/index.php/journal/article/view/279 |
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