CHARACTERISTICS OF THE BINDING BETWEEN THE ENZYME PREPARATION RAPHANUS SATIVUS L. AND NATURAL LAYERED ALUMOSILICA IN THE PRESENCE OF IRON OXIDES
IR and X-ray analyses of bentonite modified with magnetite and enzyme established the dependence of enzyme immobilization on the presence of iron oxides. IR studies have proven that the binding of the enzyme to the carrier is adsorption in nature without stable chemical interactions. This type of bi...
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| Datum: | 2023 |
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V.I.Vernadsky Institute of General and Inorganic Chemistry
2023
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oai:ojs2.1444248.nisspano.web.hosting-test.net:article-5462023-07-11T08:16:58Z CHARACTERISTICS OF THE BINDING BETWEEN THE ENZYME PREPARATION RAPHANUS SATIVUS L. AND NATURAL LAYERED ALUMOSILICA IN THE PRESENCE OF IRON OXIDES Boychuk, Oleksandr Pershina, Katherine Kolomiets, Eugene Pletenets, Yelyzaveta bentonite, magnetite, modification, Raphanus Sativus L., IR-spectroscopy, XRD-analysis. IR and X-ray analyses of bentonite modified with magnetite and enzyme established the dependence of enzyme immobilization on the presence of iron oxides. IR studies have proven that the binding of the enzyme to the carrier is adsorption in nature without stable chemical interactions. This type of binding does not significantly affect the change in the structure of the enzyme module. Characteristic bands of vibrations of amide groups of proteins were detected at 1637 cm-1 ( ν-vibrations of C=O bonds (amide I)), 713 cm-1 (δ – vibrations of O=C-N in the plane (amide IV)), which can participate in the sorption binding of the enzyme to bentonite and the SiO2 group, which is characterized by a weak connection of the outer part of protein with the formation of a surface structure, which disappears over time due to the diffusion of molecules into the pores of the mineral. Comparison of the basal distances in the samples established the following dependence: 12.43 Å bentonite+ Fe3O4+enz (48 h) > 12.38; Å natural bentonite> 12.31; Å bentonite+ Fe3O4+enz (2 hours) > 11.91; Å bentonite+ Fe3O4 , which indicates a high probability of iron compounds entering the interlayer space due to the hydrolysis of magnetite with the formation of Fe-(OH)n bonds and unstable compounds. According to the research results, a scheme of the interaction of the enzyme with modified layered iron aluminosilicate is proposed: 1 – transport of the enzyme to the surface (phase distribution boundaries); 2 – deformation of the enzyme under the action of the surface as a result of a polycondensation reaction with the formation of water molecules; 3 – entry of water molecules into the interlayer space; 4 – hydrolysis of magnetite particles; 5 – extraction of iron hydroxide from the interlayer space due to the formation of aggregates with an enzyme molecule that has a related adsorption center. V.I.Vernadsky Institute of General and Inorganic Chemistry 2023-05-25 Article Article Physical chemistry Физическая xимия Фізична xімія application/pdf https://ucj.org.ua/index.php/journal/article/view/546 10.33609/2708-129X.89.04.2023.90-101 Ukrainian Chemistry Journal; Vol 89 No 4 (2023): Ukrainian Chemistry Journal; 90-101 Украинский химический журнал; Том 89 № 4 (2023): Ukrainian Chemistry Journal; 90-101 Український хімічний журнал; Том 89 № 4 (2023): Ukrainian Chemistry Journal; 90-101 2708-129X 2708-1281 en https://ucj.org.ua/index.php/journal/article/view/546/277 |
| institution |
Ukrainian Chemistry Journal |
| baseUrl_str |
|
| datestamp_date |
2023-07-11T08:16:58Z |
| collection |
OJS |
| language |
English |
| topic_facet |
bentonite magnetite modification Raphanus Sativus L. IR-spectroscopy XRD-analysis. |
| format |
Article |
| author |
Boychuk, Oleksandr Pershina, Katherine Kolomiets, Eugene Pletenets, Yelyzaveta |
| spellingShingle |
Boychuk, Oleksandr Pershina, Katherine Kolomiets, Eugene Pletenets, Yelyzaveta CHARACTERISTICS OF THE BINDING BETWEEN THE ENZYME PREPARATION RAPHANUS SATIVUS L. AND NATURAL LAYERED ALUMOSILICA IN THE PRESENCE OF IRON OXIDES |
| author_facet |
Boychuk, Oleksandr Pershina, Katherine Kolomiets, Eugene Pletenets, Yelyzaveta |
| author_sort |
Boychuk, Oleksandr |
| title |
CHARACTERISTICS OF THE BINDING BETWEEN THE ENZYME PREPARATION RAPHANUS SATIVUS L. AND NATURAL LAYERED ALUMOSILICA IN THE PRESENCE OF IRON OXIDES |
| title_short |
CHARACTERISTICS OF THE BINDING BETWEEN THE ENZYME PREPARATION RAPHANUS SATIVUS L. AND NATURAL LAYERED ALUMOSILICA IN THE PRESENCE OF IRON OXIDES |
| title_full |
CHARACTERISTICS OF THE BINDING BETWEEN THE ENZYME PREPARATION RAPHANUS SATIVUS L. AND NATURAL LAYERED ALUMOSILICA IN THE PRESENCE OF IRON OXIDES |
| title_fullStr |
CHARACTERISTICS OF THE BINDING BETWEEN THE ENZYME PREPARATION RAPHANUS SATIVUS L. AND NATURAL LAYERED ALUMOSILICA IN THE PRESENCE OF IRON OXIDES |
| title_full_unstemmed |
CHARACTERISTICS OF THE BINDING BETWEEN THE ENZYME PREPARATION RAPHANUS SATIVUS L. AND NATURAL LAYERED ALUMOSILICA IN THE PRESENCE OF IRON OXIDES |
| title_sort |
characteristics of the binding between the enzyme preparation raphanus sativus l. and natural layered alumosilica in the presence of iron oxides |
| description |
IR and X-ray analyses of bentonite modified with magnetite and enzyme established the dependence of enzyme immobilization on the presence of iron oxides. IR studies have proven that the binding of the enzyme to the carrier is adsorption in nature without stable chemical interactions. This type of binding does not significantly affect the change in the structure of the enzyme module. Characteristic bands of vibrations of amide groups of proteins were detected at 1637 cm-1 ( ν-vibrations of C=O bonds (amide I)), 713 cm-1 (δ – vibrations of O=C-N in the plane (amide IV)), which can participate in the sorption binding of the enzyme to bentonite and the SiO2 group, which is characterized by a weak connection of the outer part of protein with the formation of a surface structure, which disappears over time due to the diffusion of molecules into the pores of the mineral. Comparison of the basal distances in the samples established the following dependence: 12.43 Å bentonite+ Fe3O4+enz (48 h) > 12.38; Å natural bentonite> 12.31; Å bentonite+ Fe3O4+enz (2 hours) > 11.91; Å bentonite+ Fe3O4 , which indicates a high probability of iron compounds entering the interlayer space due to the hydrolysis of magnetite with the formation of Fe-(OH)n bonds and unstable compounds. According to the research results, a scheme of the interaction of the enzyme with modified layered iron aluminosilicate is proposed: 1 – transport of the enzyme to the surface (phase distribution boundaries); 2 – deformation of the enzyme under the action of the surface as a result of a polycondensation reaction with the formation of water molecules; 3 – entry of water molecules into the interlayer space; 4 – hydrolysis of magnetite particles; 5 – extraction of iron hydroxide from the interlayer space due to the formation of aggregates with an enzyme molecule that has a related adsorption center. |
| publisher |
V.I.Vernadsky Institute of General and Inorganic Chemistry |
| publishDate |
2023 |
| url |
https://ucj.org.ua/index.php/journal/article/view/546 |
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