OPTIMIZATION OF SYNTHESIS CONDITIONS OF BIOCOMPATIBLE COLLOID SOLUTIONS OF COPPER METAL PARTICLES

• Date: 2025
• Main Author: Krupko, Olena
• Format: Article
• Language: English
• Published: V.I.Vernadsky Institute of General and Inorganic Chemistry 2025
• Online Access: https://ucj.org.ua/index.php/journal/article/view/753
• Journal Title: Ukrainian Chemistry Journal

The work presents a comprehensive selection of conditions for the synthesis of nanosized Copper particles in an aqueous oxidizing environment using a biocompatible amino acid – L-Сysteine as a stabilizer, a reducing agent – sodium tetraborate, and the application of the method of mathematical experimental planning the Scheffe method. The use of the mathematical planning method made it possible to predict the additive effect of the ratio between precursors in the studied medium on the value of the optical absorption edge of the obtained colloidal solutions of copper nanoparticles, their stability over time and the effect on test cultures of microorganisms P.aeruginosa, C.albicans, B.subtilitis. The ratio between the starting reagents that lead to the formation of stable colloidal solutions of copper nanoparticles at pH=6 and temperature of 20°C in an oxidizing reaction medium has been established. A mathematical model was constructed in the form of a projection onto the plane of an equilateral triangle of the dependence of the value of the optical absorption edge of colloidal solutions of metallic copper nanoparticles on the ratio between the precursors. A mathematical equation was obtained – a fourth-degree polynomial that describes the dependence of the value of the optical absorption edge of colloidal solutions of copper nanoparticles on the ratio between three independent variables – crystal-forming components of time-stable particles in the reaction medium. The antibacterial activities of a series of test solutions were investigated by the microme­thod of serial dilutions in accordance with the procedures of the European Committee for Susceptibility Testing against reference strains of bacteria (P.aeruginosa, C.albicans, B.subtilitis.). Using the Scheffe mathematical model, the concentration regions and ratios between the components of the studied system were determined, which had the highest impact on the action of test cultures of microorganisms.