ВИЯВЛЕННЯ АНТИТІЛ ПРОТИ БЕНЗО[А]ПІРЕНУ В СИРОВАТЦІ КРОВІ ХВОРИХ НА РАК ЛЕГЕНІ ЗА ДОПОМОГОЮ ІМУНОФЕРМЕНТНОГО МЕТОДУ З ВИКОРИСТАННЯМ РЕКОМБІНАНТНИХ ІДІОТИПОВИХ ТА АНТИІДІОТИПОВИХ АНТИТІЛ

• Date: 2023
• Main Authors: Studennikov, A.E., Glushkov, A.N., Ustinov, V.A.
• Format: Article
• Language: English
• Published: PH Akademperiodyka 2023
• Subjects: поліциклічні ароматичні вуглеводні; антиідіотипові антитіла; ідіотипові антитіла; IgG; ELISA; рак легені.
• Online Access: https://exp-oncology.com.ua/index.php/Exp/article/view/2021-4-14
• Journal Title: Experimental Oncology

Summary. Aim of this study was to develop and apply an ELISA method for assessment of the levels of endogenous antibodies against benzo[a]pyrene (Bp) in blood sera of lung cancer (LC) patients. Materials and Methods: ELISA was developed and applied for the detection of endogenous idiotypic and anti-idiotypic antibodies against Bp in human blood sera using recombinant human idiotypic and anti-idiotypic antibodies against Bp. Results: Serum samples of LC patients (n = 22) and healthy donors (n = 24) were analyzed by the new method. Statistical analysis showed that in sera of LC patients the levels of endogenous idiotypic and anti-idiotypic antibodies against Bp were significantly higher than in healthy donors. A logistic regression model for the LC detection utilizing such predictors as the serum levels of idiotypic and anti-idiotypic antibodies against Bp, smoking status, and age, identified LC patients with 83% specificity and 82% sensitivity. Conclusion: The proposed method could be further developed as additional lung cancer screening tool.