Microarray based gene expression profiling of advanced gall bladder cancer
Summary. Background: Gall bladder cancer (GBC) is an aggressive cancer with specific predilection like female gender and specific geographical areas, however the molecular mechanisms and factors contributing to the clinical or biological behavior are not understood. Aim: The aim of this study was to...
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| Дата: | 2023 |
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| Мова: | English |
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PH Akademperiodyka
2023
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| Назва журналу: | Experimental Oncology |
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Experimental Oncology| id |
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Experimental Oncology |
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2023-10-11T16:43:48Z |
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English |
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Cdc45 chronic cholecystitis gall bladder cancer MCM4 microarray |
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Cdc45 chronic cholecystitis gall bladder cancer MCM4 microarray Kumar, A. Gupta, R. Mathur, N. Iyer, V.K. Thulkar, S. Prasad, C.P. Das, P. Rani, L. Maqbool, M. Shukla, N.K. Pal, S. Sundar, D. Sharma, A. Microarray based gene expression profiling of advanced gall bladder cancer |
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Cdc45 chronic cholecystitis gall bladder cancer MCM4 microarray Cdc45 chronic cholecystitis gall bladder cancer MCM4 microarray |
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Article |
| author |
Kumar, A. Gupta, R. Mathur, N. Iyer, V.K. Thulkar, S. Prasad, C.P. Das, P. Rani, L. Maqbool, M. Shukla, N.K. Pal, S. Sundar, D. Sharma, A. |
| author_facet |
Kumar, A. Gupta, R. Mathur, N. Iyer, V.K. Thulkar, S. Prasad, C.P. Das, P. Rani, L. Maqbool, M. Shukla, N.K. Pal, S. Sundar, D. Sharma, A. |
| author_sort |
Kumar, A. |
| title |
Microarray based gene expression profiling of advanced gall bladder cancer |
| title_short |
Microarray based gene expression profiling of advanced gall bladder cancer |
| title_full |
Microarray based gene expression profiling of advanced gall bladder cancer |
| title_fullStr |
Microarray based gene expression profiling of advanced gall bladder cancer |
| title_full_unstemmed |
Microarray based gene expression profiling of advanced gall bladder cancer |
| title_sort |
microarray based gene expression profiling of advanced gall bladder cancer |
| title_alt |
Microarray based gene expression profiling of advanced gall bladder cancer |
| description |
Summary. Background: Gall bladder cancer (GBC) is an aggressive cancer with specific predilection like female gender and specific geographical areas, however the molecular mechanisms and factors contributing to the clinical or biological behavior are not understood. Aim: The aim of this study was to perform a comprehensive analysis of differentially expressed genes in advanced GBC and chronic cholecystitis (CC) cases. Materials and Methods: Microarray was planned on fresh specimens of advanced GBC and CC cases using single color cRNA based microarray technique (8X60K format; Agilent Technologies, USA). Twelve advanced GBC and four CC patients were included in the study. Results: Of the total of 1307 differentially expressed genes, 535 genes were significantly upregulated, while 772 genes were significantly downregulated in advanced GBC vs CC samples. Differentially expressed genes were associated with biological processes (55.03%), cellular components (31.48%), and molecular functions (13.49%) respectively. The important pathways or key processes affected were cell cycle, DNA replication, oxidative stress, gastric cancer pathway. Using in silico analysis tools, three differentially expressed genes i.e. TPX2, Cdc45 and MCM4 were selected (for their significant role in DNA replication and microtubule function) and were further validated in 20 advanced GBC cohort by immunohistochemistry. Significant positive association of Cdc45 and MCM4 proteins was found in advanced GBC cases (p = 0.043), suggesting the probable oncogenic role of Cdc45 and MCM4 proteins in advanced GBC. Conclusion: Our data demonstrate the potential regulation of Cdc45-MCM4 axis in advanced GBC tumors. Additionally, our study also revealed a range of differentially expressed genes (e.g. TPX2, AKURA etc.) between GBC and CC, and further validation of these genes might provide a potential diagnostic or therapeutic target in future. |
| publisher |
PH Akademperiodyka |
| publishDate |
2023 |
| url |
https://exp-oncology.com.ua/index.php/Exp/article/view/2020-4-12 |
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oai:ojs2.ex.aqua-time.com.ua:article-1572023-10-11T16:43:48Z Microarray based gene expression profiling of advanced gall bladder cancer Microarray based gene expression profiling of advanced gall bladder cancer Kumar, A. Gupta, R. Mathur, N. Iyer, V.K. Thulkar, S. Prasad, C.P. Das, P. Rani, L. Maqbool, M. Shukla, N.K. Pal, S. Sundar, D. Sharma, A. Cdc45, chronic cholecystitis, gall bladder cancer, MCM4, microarray Cdc45, chronic cholecystitis, gall bladder cancer, MCM4, microarray Summary. Background: Gall bladder cancer (GBC) is an aggressive cancer with specific predilection like female gender and specific geographical areas, however the molecular mechanisms and factors contributing to the clinical or biological behavior are not understood. Aim: The aim of this study was to perform a comprehensive analysis of differentially expressed genes in advanced GBC and chronic cholecystitis (CC) cases. Materials and Methods: Microarray was planned on fresh specimens of advanced GBC and CC cases using single color cRNA based microarray technique (8X60K format; Agilent Technologies, USA). Twelve advanced GBC and four CC patients were included in the study. Results: Of the total of 1307 differentially expressed genes, 535 genes were significantly upregulated, while 772 genes were significantly downregulated in advanced GBC vs CC samples. Differentially expressed genes were associated with biological processes (55.03%), cellular components (31.48%), and molecular functions (13.49%) respectively. The important pathways or key processes affected were cell cycle, DNA replication, oxidative stress, gastric cancer pathway. Using in silico analysis tools, three differentially expressed genes i.e. TPX2, Cdc45 and MCM4 were selected (for their significant role in DNA replication and microtubule function) and were further validated in 20 advanced GBC cohort by immunohistochemistry. Significant positive association of Cdc45 and MCM4 proteins was found in advanced GBC cases (p = 0.043), suggesting the probable oncogenic role of Cdc45 and MCM4 proteins in advanced GBC. Conclusion: Our data demonstrate the potential regulation of Cdc45-MCM4 axis in advanced GBC tumors. Additionally, our study also revealed a range of differentially expressed genes (e.g. TPX2, AKURA etc.) between GBC and CC, and further validation of these genes might provide a potential diagnostic or therapeutic target in future. Summary. Background: Gall bladder cancer (GBC) is an aggressive cancer with specific predilection like female gender and specific geographical areas, however the molecular mechanisms and factors contributing to the clinical or biological behavior are not understood. Aim: The aim of this study was to perform a comprehensive analysis of differentially expressed genes in advanced GBC and chronic cholecystitis (CC) cases. Materials and Methods: Microarray was planned on fresh specimens of advanced GBC and CC cases using single color cRNA based microarray technique (8X60K format; Agilent Technologies, USA). Twelve advanced GBC and four CC patients were included in the study. Results: Of the total of 1307 differentially expressed genes, 535 genes were significantly upregulated, while 772 genes were significantly downregulated in advanced GBC vs CC samples. Differentially expressed genes were associated with biological processes (55.03%), cellular components (31.48%), and molecular functions (13.49%) respectively. The important pathways or key processes affected were cell cycle, DNA replication, oxidative stress, gastric cancer pathway. Using in silico analysis tools, three differentially expressed genes i.e. TPX2, Cdc45 and MCM4 were selected (for their significant role in DNA replication and microtubule function) and were further validated in 20 advanced GBC cohort by immunohistochemistry. Significant positive association of Cdc45 and MCM4 proteins was found in advanced GBC cases (p = 0.043), suggesting the probable oncogenic role of Cdc45 and MCM4 proteins in advanced GBC. Conclusion: Our data demonstrate the potential regulation of Cdc45-MCM4 axis in advanced GBC tumors. Additionally, our study also revealed a range of differentially expressed genes (e.g. TPX2, AKURA etc.) between GBC and CC, and further validation of these genes might provide a potential diagnostic or therapeutic target in future. PH Akademperiodyka 2023-05-30 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2020-4-12 10.32471/exp-oncology.2312-8852.vol-42-no-4.15476 Experimental Oncology; Vol. 42 No. 4 (2020): Experimental Oncology; 277-284 Експериментальна онкологія; Том 42 № 4 (2020): Експериментальна онкологія; 277-284 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-42-no-4 en https://exp-oncology.com.ua/index.php/Exp/article/view/2020-4-12/2020-4-12 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/ |