Prognostic significance of microRNA-200b and ERCC1 expression in tumor cells of patients with esophageal cancer
Summary. Background: The development of malignant tumors, including esophageal cancer (EC), could be associated with impaired expression of oncosuppressive miRNA-200b and еxcision repair cross-complementing group-1 (ERCC1), a protein involved in DNA repair and alternative splicing. Aim: To investiga...
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| Datum: | 2023 |
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PH Akademperiodyka
2023
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| Назва журналу: | Experimental Oncology |
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Experimental Oncology| _version_ | 1856543491829530624 |
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| author | Kirkilevsky, S.I. Krakhmalev, P.S. Malyshok, N.V. Zadvornyi, T.V. Borikun, T.V. Yalovenko, T.M. |
| author_facet | Kirkilevsky, S.I. Krakhmalev, P.S. Malyshok, N.V. Zadvornyi, T.V. Borikun, T.V. Yalovenko, T.M. |
| author_sort | Kirkilevsky, S.I. |
| baseUrl_str | |
| collection | OJS |
| datestamp_date | 2023-10-11T16:43:26Z |
| description | Summary. Background: The development of malignant tumors, including esophageal cancer (EC), could be associated with impaired expression of oncosuppressive miRNA-200b and еxcision repair cross-complementing group-1 (ERCC1), a protein involved in DNA repair and alternative splicing. Aim: To investigate the features of expression of miRNA-200b and ERCC1 in tumor cells of patients with EC and to determine the possibility of their use for prediction of EC aggressiveness. Materials and Methods: 52 patients with EC of stages II–IV were enrolled. Expression of ERCC1 in tumor cells was assessed by immunohistochemical method, expression of miRNA-200b was evaluated by the real time reverse transcription-polymerase chain reaction. Results: The expression of miRNA-200b and ERCC1 in tumor cells of EC patients is associated with their life expectancy. The characteristic features of neoplasms in patients who died within 12 months are low expression of miRNA-200b (2.87 ± 1.65 a.u.) (ρ = –0.42; p < 0.05) and high expression of ERCC1 (191.0 ± 18.6 H-Score points) (ρ = –0.48; p < 0.05). The inverse correlations between the level of miRNA-200b expression and the tumor size were found both in the group of patients with survival < 12 months (ρ = –0.42; p < 0.05) and in the group of patients with survival > 12 months after surgery (ρ = –0.53; p < 0.05). Conclusions: The obtained data indicate the feasibility of using the expression of miRNA-200b and ERCC1 in EC cells to predict the aggressiveness of esophageal cancer. |
| first_indexed | 2025-07-17T12:16:02Z |
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| institution | Experimental Oncology |
| language | English |
| last_indexed | 2025-07-17T12:16:02Z |
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| spelling | oai:ojs2.ex.aqua-time.com.ua:article-1662023-10-11T16:43:26Z Prognostic significance of microRNA-200b and ERCC1 expression in tumor cells of patients with esophageal cancer Prognostic significance of microRNA-200b and ERCC1 expression in tumor cells of patients with esophageal cancer Kirkilevsky, S.I. Krakhmalev, P.S. Malyshok, N.V. Zadvornyi, T.V. Borikun, T.V. Yalovenko, T.M. aggressiveness of cancer, ERCC1, esophageal cancer, miRNA 200b aggressiveness of cancer, ERCC1, esophageal cancer, miRNA 200b Summary. Background: The development of malignant tumors, including esophageal cancer (EC), could be associated with impaired expression of oncosuppressive miRNA-200b and еxcision repair cross-complementing group-1 (ERCC1), a protein involved in DNA repair and alternative splicing. Aim: To investigate the features of expression of miRNA-200b and ERCC1 in tumor cells of patients with EC and to determine the possibility of their use for prediction of EC aggressiveness. Materials and Methods: 52 patients with EC of stages II–IV were enrolled. Expression of ERCC1 in tumor cells was assessed by immunohistochemical method, expression of miRNA-200b was evaluated by the real time reverse transcription-polymerase chain reaction. Results: The expression of miRNA-200b and ERCC1 in tumor cells of EC patients is associated with their life expectancy. The characteristic features of neoplasms in patients who died within 12 months are low expression of miRNA-200b (2.87 ± 1.65 a.u.) (ρ = –0.42; p < 0.05) and high expression of ERCC1 (191.0 ± 18.6 H-Score points) (ρ = –0.48; p < 0.05). The inverse correlations between the level of miRNA-200b expression and the tumor size were found both in the group of patients with survival < 12 months (ρ = –0.42; p < 0.05) and in the group of patients with survival > 12 months after surgery (ρ = –0.53; p < 0.05). Conclusions: The obtained data indicate the feasibility of using the expression of miRNA-200b and ERCC1 in EC cells to predict the aggressiveness of esophageal cancer. Summary. Background: The development of malignant tumors, including esophageal cancer (EC), could be associated with impaired expression of oncosuppressive miRNA-200b and еxcision repair cross-complementing group-1 (ERCC1), a protein involved in DNA repair and alternative splicing. Aim: To investigate the features of expression of miRNA-200b and ERCC1 in tumor cells of patients with EC and to determine the possibility of their use for prediction of EC aggressiveness. Materials and Methods: 52 patients with EC of stages II–IV were enrolled. Expression of ERCC1 in tumor cells was assessed by immunohistochemical method, expression of miRNA-200b was evaluated by the real time reverse transcription-polymerase chain reaction. Results: The expression of miRNA-200b and ERCC1 in tumor cells of EC patients is associated with their life expectancy. The characteristic features of neoplasms in patients who died within 12 months are low expression of miRNA-200b (2.87 ± 1.65 a.u.) (ρ = –0.42; p < 0.05) and high expression of ERCC1 (191.0 ± 18.6 H-Score points) (ρ = –0.48; p < 0.05). The inverse correlations between the level of miRNA-200b expression and the tumor size were found both in the group of patients with survival < 12 months (ρ = –0.42; p < 0.05) and in the group of patients with survival > 12 months after surgery (ρ = –0.53; p < 0.05). Conclusions: The obtained data indicate the feasibility of using the expression of miRNA-200b and ERCC1 in EC cells to predict the aggressiveness of esophageal cancer. PH Akademperiodyka 2023-05-31 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-3 10.32471/exp-oncology.2312-8852.vol-42-no-3.14822 Experimental Oncology; Vol. 42 No. 3 (2020): Experimental Oncology; 167-171 Експериментальна онкологія; Том 42 № 3 (2020): Експериментальна онкологія; 167-171 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-42-no-3 en https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-3/2020-3-3 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/ |
| spellingShingle | aggressiveness of cancer ERCC1 esophageal cancer miRNA 200b Kirkilevsky, S.I. Krakhmalev, P.S. Malyshok, N.V. Zadvornyi, T.V. Borikun, T.V. Yalovenko, T.M. Prognostic significance of microRNA-200b and ERCC1 expression in tumor cells of patients with esophageal cancer |
| title | Prognostic significance of microRNA-200b and ERCC1 expression in tumor cells of patients with esophageal cancer |
| title_alt | Prognostic significance of microRNA-200b and ERCC1 expression in tumor cells of patients with esophageal cancer |
| title_full | Prognostic significance of microRNA-200b and ERCC1 expression in tumor cells of patients with esophageal cancer |
| title_fullStr | Prognostic significance of microRNA-200b and ERCC1 expression in tumor cells of patients with esophageal cancer |
| title_full_unstemmed | Prognostic significance of microRNA-200b and ERCC1 expression in tumor cells of patients with esophageal cancer |
| title_short | Prognostic significance of microRNA-200b and ERCC1 expression in tumor cells of patients with esophageal cancer |
| title_sort | prognostic significance of microrna-200b and ercc1 expression in tumor cells of patients with esophageal cancer |
| topic | aggressiveness of cancer ERCC1 esophageal cancer miRNA 200b |
| topic_facet | aggressiveness of cancer ERCC1 esophageal cancer miRNA 200b aggressiveness of cancer ERCC1 esophageal cancer miRNA 200b |
| url | https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-3 |
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