Functions of tumor-associated macrophages and macrophages residing in remote anatomical niches in Ehrlich carcinoma bearing mice
Summary. Background: The impact of growing tumor on polarization and functions of tumor-associated macrophages is well known while its influence on residential macrophages occupying different anatomical niches reminds to be elucidated. Aim: To study changes in polarization and functions of macrophag...
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2023
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oai:ojs2.ex.aqua-time.com.ua:article-1702023-10-11T16:43:26Z Functions of tumor-associated macrophages and macrophages residing in remote anatomical niches in Ehrlich carcinoma bearing mice Functions of tumor-associated macrophages and macrophages residing in remote anatomical niches in Ehrlich carcinoma bearing mice Symchych, T.V. Fedosova, N.I. Chumak, A.V. Cheremshenko, N.L. Karaman, O.M. Burlaka, A.P. Voyeykova, I.M. Chekhun, V.F. Ehrlich carcinoma, functions, peritoneal macrophages, polarization, spleen macrophages, tumor-associated macrophages Ehrlich carcinoma, functions, peritoneal macrophages, polarization, spleen macrophages, tumor-associated macrophages Summary. Background: The impact of growing tumor on polarization and functions of tumor-associated macrophages is well known while its influence on residential macrophages occupying different anatomical niches reminds to be elucidated. Aim: To study changes in polarization and functions of macrophages isolated from discrete anatomical niches in tumor-bearing mice at different stages of tumor growth. Materials and Methods: Ehrlich carcinoma was transplanted intramuscularly to Balb/c male mice. On days 7, 14, 21 and 28 after tumor transplantation, macrophages from tumor tissue, peritoneal cavity and spleen were isolated and analyzed. Nitric oxide production was measured by standard Griess reaction, arginase activity was determined by the measurement of urea, reactive oxygen species production was checked using NBT dye reduction assay and electron paramagnetic resonance spectroscopy, cytotoxic activity was estimated in MTT-assay. Results: Independently of their localization in different anatomic niches, macrophages in mice with transplanted Ehrlich carcinoma gradually lose their tumoricidal activities while arginase activity is upregulated. This indicates the shift of polarization from M1-like towards M2-like phenotype. Conclusion: Our findings demonstrated that growing tumor could be able to subvert functioning of macrophages at the systemic level. Summary. Background: The impact of growing tumor on polarization and functions of tumor-associated macrophages is well known while its influence on residential macrophages occupying different anatomical niches reminds to be elucidated. Aim: To study changes in polarization and functions of macrophages isolated from discrete anatomical niches in tumor-bearing mice at different stages of tumor growth. Materials and Methods: Ehrlich carcinoma was transplanted intramuscularly to Balb/c male mice. On days 7, 14, 21 and 28 after tumor transplantation, macrophages from tumor tissue, peritoneal cavity and spleen were isolated and analyzed. Nitric oxide production was measured by standard Griess reaction, arginase activity was determined by the measurement of urea, reactive oxygen species production was checked using NBT dye reduction assay and electron paramagnetic resonance spectroscopy, cytotoxic activity was estimated in MTT-assay. Results: Independently of their localization in different anatomic niches, macrophages in mice with transplanted Ehrlich carcinoma gradually lose their tumoricidal activities while arginase activity is upregulated. This indicates the shift of polarization from M1-like towards M2-like phenotype. Conclusion: Our findings demonstrated that growing tumor could be able to subvert functioning of macrophages at the systemic level. PH Akademperiodyka 2023-05-31 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-7 10.32471/exp-oncology.2312-8852.vol-42-no-3.14928 Experimental Oncology; Vol. 42 No. 3 (2020): Experimental Oncology; 197-203 Експериментальна онкологія; Том 42 № 3 (2020): Експериментальна онкологія; 197-203 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-42-no-3 en https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-7/2020-3-7 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/ |
| institution |
Experimental Oncology |
| baseUrl_str |
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| datestamp_date |
2023-10-11T16:43:26Z |
| collection |
OJS |
| language |
English |
| topic |
Ehrlich carcinoma functions peritoneal macrophages polarization spleen macrophages tumor-associated macrophages |
| spellingShingle |
Ehrlich carcinoma functions peritoneal macrophages polarization spleen macrophages tumor-associated macrophages Symchych, T.V. Fedosova, N.I. Chumak, A.V. Cheremshenko, N.L. Karaman, O.M. Burlaka, A.P. Voyeykova, I.M. Chekhun, V.F. Functions of tumor-associated macrophages and macrophages residing in remote anatomical niches in Ehrlich carcinoma bearing mice |
| topic_facet |
Ehrlich carcinoma functions peritoneal macrophages polarization spleen macrophages tumor-associated macrophages Ehrlich carcinoma functions peritoneal macrophages polarization spleen macrophages tumor-associated macrophages |
| format |
Article |
| author |
Symchych, T.V. Fedosova, N.I. Chumak, A.V. Cheremshenko, N.L. Karaman, O.M. Burlaka, A.P. Voyeykova, I.M. Chekhun, V.F. |
| author_facet |
Symchych, T.V. Fedosova, N.I. Chumak, A.V. Cheremshenko, N.L. Karaman, O.M. Burlaka, A.P. Voyeykova, I.M. Chekhun, V.F. |
| author_sort |
Symchych, T.V. |
| title |
Functions of tumor-associated macrophages and macrophages residing in remote anatomical niches in Ehrlich carcinoma bearing mice |
| title_short |
Functions of tumor-associated macrophages and macrophages residing in remote anatomical niches in Ehrlich carcinoma bearing mice |
| title_full |
Functions of tumor-associated macrophages and macrophages residing in remote anatomical niches in Ehrlich carcinoma bearing mice |
| title_fullStr |
Functions of tumor-associated macrophages and macrophages residing in remote anatomical niches in Ehrlich carcinoma bearing mice |
| title_full_unstemmed |
Functions of tumor-associated macrophages and macrophages residing in remote anatomical niches in Ehrlich carcinoma bearing mice |
| title_sort |
functions of tumor-associated macrophages and macrophages residing in remote anatomical niches in ehrlich carcinoma bearing mice |
| title_alt |
Functions of tumor-associated macrophages and macrophages residing in remote anatomical niches in Ehrlich carcinoma bearing mice |
| description |
Summary. Background: The impact of growing tumor on polarization and functions of tumor-associated macrophages is well known while its influence on residential macrophages occupying different anatomical niches reminds to be elucidated. Aim: To study changes in polarization and functions of macrophages isolated from discrete anatomical niches in tumor-bearing mice at different stages of tumor growth. Materials and Methods: Ehrlich carcinoma was transplanted intramuscularly to Balb/c male mice. On days 7, 14, 21 and 28 after tumor transplantation, macrophages from tumor tissue, peritoneal cavity and spleen were isolated and analyzed. Nitric oxide production was measured by standard Griess reaction, arginase activity was determined by the measurement of urea, reactive oxygen species production was checked using NBT dye reduction assay and electron paramagnetic resonance spectroscopy, cytotoxic activity was estimated in MTT-assay. Results: Independently of their localization in different anatomic niches, macrophages in mice with transplanted Ehrlich carcinoma gradually lose their tumoricidal activities while arginase activity is upregulated. This indicates the shift of polarization from M1-like towards M2-like phenotype. Conclusion: Our findings demonstrated that growing tumor could be able to subvert functioning of macrophages at the systemic level. |
| publisher |
PH Akademperiodyka |
| publishDate |
2023 |
| url |
https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-7 |
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