Antiproliferative and apoptotic effect of ethanolic extract of Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer

Antiproliferative and apoptotic effect of ethanolic extract of Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer

Summary. Background: Pancreatic cancer is an aggressive malignancy with poor prognosis. New options for its treatment including the use of the substances of natural origin are now on the agenda. Aim: An attempt has been taken to examine the effect of ethanolic extract (EE) of edible mushroom Calocyb...

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Datum:2023
Hauptverfasser: Ghosh, S.K., Sanyal, T.
Format: Artikel
Sprache:English
Veröffentlicht: PH Akademperiodyka 2023
Schlagworte:
anticancer agent
apoptosis
cytotoxicity
ethanolic extract
MIAPaCa2
PANC-1
Online Zugang:https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-12
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Experimental Oncology
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record_format ojs
institution Experimental Oncology
baseUrl_str
datestamp_date 2023-10-11T16:43:26Z
collection OJS
language English
topic anticancer agent
apoptosis
cytotoxicity
ethanolic extract
MIAPaCa2
PANC-1
spellingShingle anticancer agent
apoptosis
cytotoxicity
ethanolic extract
MIAPaCa2
PANC-1
Ghosh, S.K.
Sanyal, T.
Antiproliferative and apoptotic effect of ethanolic extract of Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer
topic_facet anticancer agent
apoptosis
cytotoxicity
ethanolic extract
MIAPaCa2
PANC-1
anticancer agent
apoptosis
cytotoxicity
ethanolic extract
MIAPaCa2
PANC-1
format Article
author Ghosh, S.K.
Sanyal, T.
author_facet Ghosh, S.K.
Sanyal, T.
author_sort Ghosh, S.K.
title Antiproliferative and apoptotic effect of ethanolic extract of Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer
title_short Antiproliferative and apoptotic effect of ethanolic extract of Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer
title_full Antiproliferative and apoptotic effect of ethanolic extract of Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer
title_fullStr Antiproliferative and apoptotic effect of ethanolic extract of Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer
title_full_unstemmed Antiproliferative and apoptotic effect of ethanolic extract of Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer
title_sort antiproliferative and apoptotic effect of ethanolic extract of calocybe indica on panc-1 and miapaca2 cell lines of pancreatic cancer
title_alt Antiproliferative and apoptotic effect of ethanolic extract of Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer
description Summary. Background: Pancreatic cancer is an aggressive malignancy with poor prognosis. New options for its treatment including the use of the substances of natural origin are now on the agenda. Aim: An attempt has been taken to examine the effect of ethanolic extract (EE) of edible mushroom Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer in vitro. Materials and Methods: Study of cell morphology, evaluation of apoptosis by DAPI staining under florescence microscope, anti-proliferative study by MTT assay, lactate dehydrogenase assay, pro-apoptotic and anti-apoptotic protein detection by Western blotting were performed to test effects of Calocybe indica EE on the cells of these two lines. Effect on in vitro migration was studied by scratch method. Results: Both cell lines treated with 250 or 500 µg/mL of EE underwent morphological changes — became more round and shrunken, with membrane blebbing and the reduced cell confluence. The nuclei of treated cells became condensed and fragmented. The percentage of apoptotic cells increased as EE concentration increased from 100 µg/mL to 500 µg/mL. Anti-proliferative effect was recorded at all concentrations within 100–750 µg/mL. The effect EE on lactate dehydrogenase leakage was concentration-dependent in both cell lines. Western blotting showed that caspase-3 and -9, and p53 protein levels were increased and BcL2 protein was decreased after treatment of the cells with EE compared to the control. PANC-1 cell migration was inhibited by 80.12 ± 4.25% after 24 h treatment of the cells with 500 µg/mL EE compared to the control. Conclusions: EE of Calocybe indica inhibits the growth and induces apoptosis in pancreatic cancer cell lines in vitro.
publisher PH Akademperiodyka
publishDate 2023
url https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-12
work_keys_str_mv AT ghoshsk antiproliferativeandapoptoticeffectofethanolicextractofcalocybeindicaonpanc1andmiapaca2celllinesofpancreaticcancer
AT sanyalt antiproliferativeandapoptoticeffectofethanolicextractofcalocybeindicaonpanc1andmiapaca2celllinesofpancreaticcancer
first_indexed 2025-07-17T12:16:08Z
last_indexed 2025-07-17T12:16:08Z
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spelling oai:ojs2.ex.aqua-time.com.ua:article-1752023-10-11T16:43:26Z Antiproliferative and apoptotic effect of ethanolic extract of Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer Antiproliferative and apoptotic effect of ethanolic extract of Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer Ghosh, S.K. Sanyal, T. anticancer agent, apoptosis, cytotoxicity, ethanolic extract, MIAPaCa2, PANC-1 anticancer agent, apoptosis, cytotoxicity, ethanolic extract, MIAPaCa2, PANC-1 Summary. Background: Pancreatic cancer is an aggressive malignancy with poor prognosis. New options for its treatment including the use of the substances of natural origin are now on the agenda. Aim: An attempt has been taken to examine the effect of ethanolic extract (EE) of edible mushroom Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer in vitro. Materials and Methods: Study of cell morphology, evaluation of apoptosis by DAPI staining under florescence microscope, anti-proliferative study by MTT assay, lactate dehydrogenase assay, pro-apoptotic and anti-apoptotic protein detection by Western blotting were performed to test effects of Calocybe indica EE on the cells of these two lines. Effect on in vitro migration was studied by scratch method. Results: Both cell lines treated with 250 or 500 µg/mL of EE underwent morphological changes — became more round and shrunken, with membrane blebbing and the reduced cell confluence. The nuclei of treated cells became condensed and fragmented. The percentage of apoptotic cells increased as EE concentration increased from 100 µg/mL to 500 µg/mL. Anti-proliferative effect was recorded at all concentrations within 100–750 µg/mL. The effect EE on lactate dehydrogenase leakage was concentration-dependent in both cell lines. Western blotting showed that caspase-3 and -9, and p53 protein levels were increased and BcL2 protein was decreased after treatment of the cells with EE compared to the control. PANC-1 cell migration was inhibited by 80.12 ± 4.25% after 24 h treatment of the cells with 500 µg/mL EE compared to the control. Conclusions: EE of Calocybe indica inhibits the growth and induces apoptosis in pancreatic cancer cell lines in vitro. Summary. Background: Pancreatic cancer is an aggressive malignancy with poor prognosis. New options for its treatment including the use of the substances of natural origin are now on the agenda. Aim: An attempt has been taken to examine the effect of ethanolic extract (EE) of edible mushroom Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer in vitro. Materials and Methods: Study of cell morphology, evaluation of apoptosis by DAPI staining under florescence microscope, anti-proliferative study by MTT assay, lactate dehydrogenase assay, pro-apoptotic and anti-apoptotic protein detection by Western blotting were performed to test effects of Calocybe indica EE on the cells of these two lines. Effect on in vitro migration was studied by scratch method. Results: Both cell lines treated with 250 or 500 µg/mL of EE underwent morphological changes — became more round and shrunken, with membrane blebbing and the reduced cell confluence. The nuclei of treated cells became condensed and fragmented. The percentage of apoptotic cells increased as EE concentration increased from 100 µg/mL to 500 µg/mL. Anti-proliferative effect was recorded at all concentrations within 100–750 µg/mL. The effect EE on lactate dehydrogenase leakage was concentration-dependent in both cell lines. Western blotting showed that caspase-3 and -9, and p53 protein levels were increased and BcL2 protein was decreased after treatment of the cells with EE compared to the control. PANC-1 cell migration was inhibited by 80.12 ± 4.25% after 24 h treatment of the cells with 500 µg/mL EE compared to the control. Conclusions: EE of Calocybe indica inhibits the growth and induces apoptosis in pancreatic cancer cell lines in vitro. PH Akademperiodyka 2023-05-31 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-12 10.32471/exp-oncology.2312-8852.vol-42-no-3.15086 Experimental Oncology; Vol. 42 No. 3 (2020): Experimental Oncology; 178-182 Експериментальна онкологія; Том 42 № 3 (2020): Експериментальна онкологія; 178-182 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-42-no-3 en https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-12/2020-3-12 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/

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