Osteopontin siRNA does not confer resistance to toxic effects of parthenolide in Jurkat cells

Osteopontin siRNA does not confer resistance to toxic effects of parthenolide in Jurkat cells

Summary. Background: Osteopontin (OPN) plays a critical role in cell proliferation and drug resistance in cancer treatment and hematological malignancies. In T cell acute lymphoblastic leukemia, most initial therapies can induce remission while some patients then relapse and do not respond...

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Datum:2023
Hauptverfasser: Mehri, S., Mohammadi, S., Nikbakht, M., Sahmani, M., Zahedpanah, M.
Format: Artikel
Sprache:English
Veröffentlicht: PH Akademperiodyka 2023
Schlagworte:
Jurkat cells
osteopontin
parthenolide
Online Zugang:https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-15
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Experimental Oncology
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spelling oai:ojs2.ex.aqua-time.com.ua:article-1782023-10-11T16:43:26Z Osteopontin siRNA does not confer resistance to toxic effects of parthenolide in Jurkat cells Osteopontin siRNA does not confer resistance to toxic effects of parthenolide in Jurkat cells Mehri, S. Mohammadi, S. Nikbakht, M. Sahmani, M. Zahedpanah, M. Jurkat cells, osteopontin, parthenolide Jurkat cells, osteopontin, parthenolide Summary. Background: Osteopontin (OPN) plays a critical role in cell proliferation and drug resistance in cancer treatment and hematological malignancies. In T cell acute lymphoblastic leukemia, most initial therapies can induce remission while some patients then relapse and do not respond well to chemotherapy. The sesquiterpene lactone parthenolide (PTL) can induce apoptosis in a variety of cancer cell lines via inhibition of pro-inflammatory transcription factor nuclear factor kappa B and has anti-tumor activity in acute lymphoblastic leukemia treatment. Aim: To study the role of OPN in conferring in vitro resistance to PTL in Jurkat cells. Methods: Jurkat cells were cultured with 8–20 μm PTL for 48 h. Transfection with OPN siRNA was provided. Apoptosis assays were performed with Annexin V-Alexa Fluor-488/PI. Quantitative real-time polymerase chain reaction was used to measure OPN gene expression using the 2-2-ΔΔCt method. Results: PTL has cytotoxic and apoptotic effect on Jurkat cells with IC50 values of 16.1 μm, and growth inhibition effect of PTL does not differ significantly in combination with OPN-siRNA. OPN gene expression is not affected by PTL. Conclusions: Parthenolide induces apoptosis in Jurkat cells, but inhibition of osteopontin gene expression with siRNA does not reduce apoptotic effect of parthenolide. Summary. Background: Osteopontin (OPN) plays a critical role in cell proliferation and drug resistance in cancer treatment and hematological malignancies. In T cell acute lymphoblastic leukemia, most initial therapies can induce remission while some patients then relapse and do not respond well to chemotherapy. The sesquiterpene lactone parthenolide (PTL) can induce apoptosis in a variety of cancer cell lines via inhibition of pro-inflammatory transcription factor nuclear factor kappa B and has anti-tumor activity in acute lymphoblastic leukemia treatment. Aim: To study the role of OPN in conferring in vitro resistance to PTL in Jurkat cells. Methods: Jurkat cells were cultured with 8–20 μm PTL for 48 h. Transfection with OPN siRNA was provided. Apoptosis assays were performed with Annexin V-Alexa Fluor-488/PI. Quantitative real-time polymerase chain reaction was used to measure OPN gene expression using the 2-2-ΔΔCt method. Results: PTL has cytotoxic and apoptotic effect on Jurkat cells with IC50 values of 16.1 μm, and growth inhibition effect of PTL does not differ significantly in combination with OPN-siRNA. OPN gene expression is not affected by PTL. Conclusions: Parthenolide induces apoptosis in Jurkat cells, but inhibition of osteopontin gene expression with siRNA does not reduce apoptotic effect of parthenolide. PH Akademperiodyka 2023-05-31 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-15 10.32471/exp-oncology.2312-8852.vol-42-no-3.15180 Experimental Oncology; Vol. 42 No. 3 (2020): Experimental Oncology; 188-191 Експериментальна онкологія; Том 42 № 3 (2020): Експериментальна онкологія; 188-191 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-42-no-3 en https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-15/2020-3-15 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/
institution Experimental Oncology
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datestamp_date 2023-10-11T16:43:26Z
collection OJS
language English
topic Jurkat cells
osteopontin
parthenolide
spellingShingle Jurkat cells
osteopontin
parthenolide
Mehri, S.
Mohammadi, S.
Nikbakht, M.
Sahmani, M.
Zahedpanah, M.
Osteopontin siRNA does not confer resistance to toxic effects of parthenolide in Jurkat cells
topic_facet Jurkat cells
osteopontin
parthenolide
Jurkat cells
osteopontin
parthenolide
format Article
author Mehri, S.
Mohammadi, S.
Nikbakht, M.
Sahmani, M.
Zahedpanah, M.
author_facet Mehri, S.
Mohammadi, S.
Nikbakht, M.
Sahmani, M.
Zahedpanah, M.
author_sort Mehri, S.
title Osteopontin siRNA does not confer resistance to toxic effects of parthenolide in Jurkat cells
title_short Osteopontin siRNA does not confer resistance to toxic effects of parthenolide in Jurkat cells
title_full Osteopontin siRNA does not confer resistance to toxic effects of parthenolide in Jurkat cells
title_fullStr Osteopontin siRNA does not confer resistance to toxic effects of parthenolide in Jurkat cells
title_full_unstemmed Osteopontin siRNA does not confer resistance to toxic effects of parthenolide in Jurkat cells
title_sort osteopontin sirna does not confer resistance to toxic effects of parthenolide in jurkat cells
title_alt Osteopontin siRNA does not confer resistance to toxic effects of parthenolide in Jurkat cells
description Summary. Background: Osteopontin (OPN) plays a critical role in cell proliferation and drug resistance in cancer treatment and hematological malignancies. In T cell acute lymphoblastic leukemia, most initial therapies can induce remission while some patients then relapse and do not respond well to chemotherapy. The sesquiterpene lactone parthenolide (PTL) can induce apoptosis in a variety of cancer cell lines via inhibition of pro-inflammatory transcription factor nuclear factor kappa B and has anti-tumor activity in acute lymphoblastic leukemia treatment. Aim: To study the role of OPN in conferring in vitro resistance to PTL in Jurkat cells. Methods: Jurkat cells were cultured with 8–20 μm PTL for 48 h. Transfection with OPN siRNA was provided. Apoptosis assays were performed with Annexin V-Alexa Fluor-488/PI. Quantitative real-time polymerase chain reaction was used to measure OPN gene expression using the 2-2-ΔΔCt method. Results: PTL has cytotoxic and apoptotic effect on Jurkat cells with IC50 values of 16.1 μm, and growth inhibition effect of PTL does not differ significantly in combination with OPN-siRNA. OPN gene expression is not affected by PTL. Conclusions: Parthenolide induces apoptosis in Jurkat cells, but inhibition of osteopontin gene expression with siRNA does not reduce apoptotic effect of parthenolide.
publisher PH Akademperiodyka
publishDate 2023
url https://exp-oncology.com.ua/index.php/Exp/article/view/2020-3-15
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last_indexed 2025-07-17T12:16:10Z
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