Inhibition of USP1, a new partner of Bcr-Abl, results in decrease of Bcr-Abl level in K562 cells
Summary. Aim: To analyze interaction of ubiquitin specific peptidase 1 (USP1) with Bcr-Abl and to assess the relation between USP1 functional activity and Bcr-Abl expression in K562 chronic myeloid leukemia cells. Materials and Methods: The interaction between USP1 and Bcr-Abl in K562&n...
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| Datum: | 2023 |
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| Sprache: | Englisch |
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PH Akademperiodyka
2023
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| Назва журналу: | Experimental Oncology |
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Experimental Oncology| _version_ | 1856543504925196288 |
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| author | Antonenko, S.V. Telegeev, G.D. |
| author_facet | Antonenko, S.V. Telegeev, G.D. |
| author_sort | Antonenko, S.V. |
| baseUrl_str | |
| collection | OJS |
| datestamp_date | 2023-10-11T16:43:03Z |
| description | Summary. Aim: To analyze interaction of ubiquitin specific peptidase 1 (USP1) with Bcr-Abl and to assess the relation between USP1 functional activity and Bcr-Abl expression in K562 chronic myeloid leukemia cells. Materials and Methods: The interaction between USP1 and Bcr-Abl in K562 cells was analyzed by co-immunoprecipitation, Western blot analysis, and confocal microscopy. Results: A direct interaction between Bcr-Abl oncoprotein and USP1 protein in K562 cells was established by co-immunoprecipitation. Immunofluorescence analysis and confocal microscopy revealed that Bcr-Abl/USP1 protein complex is formed in the cell nucleus. The inhibition of USP1 protein activity by ML323 reduced the level of Bcr-Abl oncoprotein in K562 cells. Conclusions: USP1 protein has been identified as a new protein partner of Bcr-Abl oncoprotein in chronic myeloid leukemia. The relationship between the functional activity of USP1 protein and the level of Bcr-Abl oncoprotein has been demonstrated, suggesting that the targeted inhibition of USP1 activity could be a challenging approach for reducing Bcr-Abl expression. |
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| format | Article |
| id | oai:ojs2.ex.aqua-time.com.ua:article-193 |
| institution | Experimental Oncology |
| language | English |
| last_indexed | 2025-07-17T12:16:23Z |
| publishDate | 2023 |
| publisher | PH Akademperiodyka |
| record_format | ojs |
| spelling | oai:ojs2.ex.aqua-time.com.ua:article-1932023-10-11T16:43:03Z Inhibition of USP1, a new partner of Bcr-Abl, results in decrease of Bcr-Abl level in K562 cells Inhibition of USP1, a new partner of Bcr-Abl, results in decrease of Bcr-Abl level in K562 cells Antonenko, S.V. Telegeev, G.D. Bcr-Abl oncoprotein, chronic myeloid leukemia, K562 cells, ML323 inhibitor, USP1 protein Bcr-Abl oncoprotein, chronic myeloid leukemia, K562 cells, ML323 inhibitor, USP1 protein Summary. Aim: To analyze interaction of ubiquitin specific peptidase 1 (USP1) with Bcr-Abl and to assess the relation between USP1 functional activity and Bcr-Abl expression in K562 chronic myeloid leukemia cells. Materials and Methods: The interaction between USP1 and Bcr-Abl in K562 cells was analyzed by co-immunoprecipitation, Western blot analysis, and confocal microscopy. Results: A direct interaction between Bcr-Abl oncoprotein and USP1 protein in K562 cells was established by co-immunoprecipitation. Immunofluorescence analysis and confocal microscopy revealed that Bcr-Abl/USP1 protein complex is formed in the cell nucleus. The inhibition of USP1 protein activity by ML323 reduced the level of Bcr-Abl oncoprotein in K562 cells. Conclusions: USP1 protein has been identified as a new protein partner of Bcr-Abl oncoprotein in chronic myeloid leukemia. The relationship between the functional activity of USP1 protein and the level of Bcr-Abl oncoprotein has been demonstrated, suggesting that the targeted inhibition of USP1 activity could be a challenging approach for reducing Bcr-Abl expression. Summary. Aim: To analyze interaction of ubiquitin specific peptidase 1 (USP1) with Bcr-Abl and to assess the relation between USP1 functional activity and Bcr-Abl expression in K562 chronic myeloid leukemia cells. Materials and Methods: The interaction between USP1 and Bcr-Abl in K562 cells was analyzed by co-immunoprecipitation, Western blot analysis, and confocal microscopy. Results: A direct interaction between Bcr-Abl oncoprotein and USP1 protein in K562 cells was established by co-immunoprecipitation. Immunofluorescence analysis and confocal microscopy revealed that Bcr-Abl/USP1 protein complex is formed in the cell nucleus. The inhibition of USP1 protein activity by ML323 reduced the level of Bcr-Abl oncoprotein in K562 cells. Conclusions: USP1 protein has been identified as a new protein partner of Bcr-Abl oncoprotein in chronic myeloid leukemia. The relationship between the functional activity of USP1 protein and the level of Bcr-Abl oncoprotein has been demonstrated, suggesting that the targeted inhibition of USP1 activity could be a challenging approach for reducing Bcr-Abl expression. PH Akademperiodyka 2023-06-01 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2020-2-10 10.32471/exp-oncology.2312-8852.vol-42-no-2.14533 Experimental Oncology; Vol. 42 No. 2 (2020): Experimental Oncology; 109-114 Експериментальна онкологія; Том 42 № 2 (2020): Експериментальна онкологія; 109-114 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-42-no-2 en https://exp-oncology.com.ua/index.php/Exp/article/view/2020-2-10/2020-2-10 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/ |
| spellingShingle | Bcr-Abl oncoprotein chronic myeloid leukemia K562 cells ML323 inhibitor USP1 protein Antonenko, S.V. Telegeev, G.D. Inhibition of USP1, a new partner of Bcr-Abl, results in decrease of Bcr-Abl level in K562 cells |
| title | Inhibition of USP1, a new partner of Bcr-Abl, results in decrease of Bcr-Abl level in K562 cells |
| title_alt | Inhibition of USP1, a new partner of Bcr-Abl, results in decrease of Bcr-Abl level in K562 cells |
| title_full | Inhibition of USP1, a new partner of Bcr-Abl, results in decrease of Bcr-Abl level in K562 cells |
| title_fullStr | Inhibition of USP1, a new partner of Bcr-Abl, results in decrease of Bcr-Abl level in K562 cells |
| title_full_unstemmed | Inhibition of USP1, a new partner of Bcr-Abl, results in decrease of Bcr-Abl level in K562 cells |
| title_short | Inhibition of USP1, a new partner of Bcr-Abl, results in decrease of Bcr-Abl level in K562 cells |
| title_sort | inhibition of usp1, a new partner of bcr-abl, results in decrease of bcr-abl level in k562 cells |
| topic | Bcr-Abl oncoprotein chronic myeloid leukemia K562 cells ML323 inhibitor USP1 protein |
| topic_facet | Bcr-Abl oncoprotein chronic myeloid leukemia K562 cells ML323 inhibitor USP1 protein Bcr-Abl oncoprotein chronic myeloid leukemia K562 cells ML323 inhibitor USP1 protein |
| url | https://exp-oncology.com.ua/index.php/Exp/article/view/2020-2-10 |
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