Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro
Summary. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a member of the epidermal growth factor family and has a variety of physiological and pathophysiological functions. Also, HB-EGF plays a pivotal role in progression of different tumors. So, HB-EGF seems to be a target mo...
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| Date: | 2023 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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PH Akademperiodyka
2023
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| Online Access: | https://exp-oncology.com.ua/index.php/Exp/article/view/2020-1-8 |
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| Journal Title: | Experimental Oncology |
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Experimental Oncology| _version_ | 1856543510635741184 |
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| author | Siromolot, A.A. Krynina, O.I. Kolybo, D.V. Komisarenko, S.V. |
| author_facet | Siromolot, A.A. Krynina, O.I. Kolybo, D.V. Komisarenko, S.V. |
| author_sort | Siromolot, A.A. |
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| collection | OJS |
| datestamp_date | 2023-10-11T16:42:32Z |
| description | Summary. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a member of the epidermal growth factor family and has a variety of physiological and pathophysiological functions. Also, HB-EGF plays a pivotal role in progression of different tumors. So, HB-EGF seems to be a target molecule for the treatment of some cancer types. Aim: To obtain HB-EGF neutralizing polyclonal antibodies and test their anti-proliferative properties in vitro. Materials and Methods: Lab rabbits and mice were used for immunization with recombinant HB-EGF. The effect of generated polyclonal antibodies on viability and apoptosis of human epidermoid carcinoma derived A431 cell line was assessed using MTT and Annexin V-propidium iodide assays. Results: Rabbit polyclonal anti-HB-EGF serum could block binding of soluble HB-EGF to epidermal growth factor receptor/human epidermal growth factor receptor. Also, anti-HB-EGF antibodies could bind to surface of A431 cells which express abnormally high levels of membrane bound proHB-EGF and its receptor. It has been shown that immune serum with polyclonal antibodies against HB-EGF was able to block the mitogenic activation of the cells with HB-EGF and cause apoptotic cell death. Conclusion: Inhibition of HB-EGF activity with neutralizing polyclonal antibodies can effectively inhibit mitogenic activation and cause apoptosis of cancer cells with significant epidermal growth factor receptor overexpression. |
| first_indexed | 2025-07-17T12:16:30Z |
| format | Article |
| id | oai:ojs2.ex.aqua-time.com.ua:article-204 |
| institution | Experimental Oncology |
| language | English |
| last_indexed | 2025-07-17T12:16:30Z |
| publishDate | 2023 |
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| spelling | oai:ojs2.ex.aqua-time.com.ua:article-2042023-10-11T16:42:32Z Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro Siromolot, A.A. Krynina, O.I. Kolybo, D.V. Komisarenko, S.V. antibodies., cancer treatment., HB-EGF, mitogenic activation, receptors antibodies., cancer treatment., HB-EGF, mitogenic activation, receptors Summary. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a member of the epidermal growth factor family and has a variety of physiological and pathophysiological functions. Also, HB-EGF plays a pivotal role in progression of different tumors. So, HB-EGF seems to be a target molecule for the treatment of some cancer types. Aim: To obtain HB-EGF neutralizing polyclonal antibodies and test their anti-proliferative properties in vitro. Materials and Methods: Lab rabbits and mice were used for immunization with recombinant HB-EGF. The effect of generated polyclonal antibodies on viability and apoptosis of human epidermoid carcinoma derived A431 cell line was assessed using MTT and Annexin V-propidium iodide assays. Results: Rabbit polyclonal anti-HB-EGF serum could block binding of soluble HB-EGF to epidermal growth factor receptor/human epidermal growth factor receptor. Also, anti-HB-EGF antibodies could bind to surface of A431 cells which express abnormally high levels of membrane bound proHB-EGF and its receptor. It has been shown that immune serum with polyclonal antibodies against HB-EGF was able to block the mitogenic activation of the cells with HB-EGF and cause apoptotic cell death. Conclusion: Inhibition of HB-EGF activity with neutralizing polyclonal antibodies can effectively inhibit mitogenic activation and cause apoptosis of cancer cells with significant epidermal growth factor receptor overexpression. Summary. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a member of the epidermal growth factor family and has a variety of physiological and pathophysiological functions. Also, HB-EGF plays a pivotal role in progression of different tumors. So, HB-EGF seems to be a target molecule for the treatment of some cancer types. Aim: To obtain HB-EGF neutralizing polyclonal antibodies and test their anti-proliferative properties in vitro. Materials and Methods: Lab rabbits and mice were used for immunization with recombinant HB-EGF. The effect of generated polyclonal antibodies on viability and apoptosis of human epidermoid carcinoma derived A431 cell line was assessed using MTT and Annexin V-propidium iodide assays. Results: Rabbit polyclonal anti-HB-EGF serum could block binding of soluble HB-EGF to epidermal growth factor receptor/human epidermal growth factor receptor. Also, anti-HB-EGF antibodies could bind to surface of A431 cells which express abnormally high levels of membrane bound proHB-EGF and its receptor. It has been shown that immune serum with polyclonal antibodies against HB-EGF was able to block the mitogenic activation of the cells with HB-EGF and cause apoptotic cell death. Conclusion: Inhibition of HB-EGF activity with neutralizing polyclonal antibodies can effectively inhibit mitogenic activation and cause apoptosis of cancer cells with significant epidermal growth factor receptor overexpression. PH Akademperiodyka 2023-06-01 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2020-1-8 10.32471/exp-oncology.2312-8852.vol-42-no-1.14145 Experimental Oncology; Vol. 42 No. 1 (2020): Experimental Oncology; 25-30 Експериментальна онкологія; Том 42 № 1 (2020): Експериментальна онкологія; 25-30 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-42-no-1 en https://exp-oncology.com.ua/index.php/Exp/article/view/2020-1-8/2020-1-8 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/ |
| spellingShingle | antibodies. cancer treatment. HB-EGF mitogenic activation receptors Siromolot, A.A. Krynina, O.I. Kolybo, D.V. Komisarenko, S.V. Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro |
| title | Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro |
| title_alt | Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro |
| title_full | Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro |
| title_fullStr | Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro |
| title_full_unstemmed | Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro |
| title_short | Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro |
| title_sort | antiproliferative and apoptotic effects of anti-human hb-egf neutralizing polyclonal antibodies in vitro |
| topic | antibodies. cancer treatment. HB-EGF mitogenic activation receptors |
| topic_facet | antibodies. cancer treatment. HB-EGF mitogenic activation receptors antibodies. cancer treatment. HB-EGF mitogenic activation receptors |
| url | https://exp-oncology.com.ua/index.php/Exp/article/view/2020-1-8 |
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