Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro

Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro

Summary. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a member of the epidermal growth factor family and has a variety of physiological and pathophysiological functions. Also, HB-EGF plays a pivotal role in progression of different tumors. So, HB-EGF seems to be a target mo...

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Bibliographic Details
Date:2023
Main Authors: Siromolot, A.A., Krynina, O.I., Kolybo, D.V., Komisarenko, S.V.
Format: Article
Language:English
Published: PH Akademperiodyka 2023
Subjects:
antibodies.
cancer treatment.
HB-EGF
mitogenic activation
receptors
Online Access:https://exp-oncology.com.ua/index.php/Exp/article/view/2020-1-8
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Journal Title:Experimental Oncology

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Experimental Oncology
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spelling oai:ojs2.ex.aqua-time.com.ua:article-2042023-10-11T16:42:32Z Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro Siromolot, A.A. Krynina, O.I. Kolybo, D.V. Komisarenko, S.V. antibodies., cancer treatment., HB-EGF, mitogenic activation, receptors antibodies., cancer treatment., HB-EGF, mitogenic activation, receptors Summary. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a member of the epidermal growth factor family and has a variety of physiological and pathophysiological functions. Also, HB-EGF plays a pivotal role in progression of different tumors. So, HB-EGF seems to be a target molecule for the treatment of some cancer types. Aim: To obtain HB-EGF neutralizing polyclonal antibodies and test their anti-proliferative properties in vitro. Materials and Methods: Lab rabbits and mice were used for immunization with recombinant HB-EGF. The effect of generated polyclonal antibodies on viability and apoptosis of human epidermoid carcinoma derived A431 cell line was assessed using MTT and Annexin V-propidium iodide assays. Results: Rabbit polyclonal anti-HB-EGF serum could block binding of soluble HB-EGF to epidermal growth factor receptor/human epidermal growth factor receptor. Also, anti-HB-EGF antibodies could bind to surface of A431 cells which express abnormally high levels of membrane bound proHB-EGF and its receptor. It has been shown that immune serum with polyclonal antibodies against HB-EGF was able to block the mitogenic activation of the cells with HB-EGF and cause apoptotic cell death. Conclusion: Inhibition of HB-EGF activity with neutralizing polyclonal antibodies can effectively inhibit mitogenic activation and cause apoptosis of cancer cells with significant epidermal growth factor receptor overexpression. Summary. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a member of the epidermal growth factor family and has a variety of physiological and pathophysiological functions. Also, HB-EGF plays a pivotal role in progression of different tumors. So, HB-EGF seems to be a target molecule for the treatment of some cancer types. Aim: To obtain HB-EGF neutralizing polyclonal antibodies and test their anti-proliferative properties in vitro. Materials and Methods: Lab rabbits and mice were used for immunization with recombinant HB-EGF. The effect of generated polyclonal antibodies on viability and apoptosis of human epidermoid carcinoma derived A431 cell line was assessed using MTT and Annexin V-propidium iodide assays. Results: Rabbit polyclonal anti-HB-EGF serum could block binding of soluble HB-EGF to epidermal growth factor receptor/human epidermal growth factor receptor. Also, anti-HB-EGF antibodies could bind to surface of A431 cells which express abnormally high levels of membrane bound proHB-EGF and its receptor. It has been shown that immune serum with polyclonal antibodies against HB-EGF was able to block the mitogenic activation of the cells with HB-EGF and cause apoptotic cell death. Conclusion: Inhibition of HB-EGF activity with neutralizing polyclonal antibodies can effectively inhibit mitogenic activation and cause apoptosis of cancer cells with significant epidermal growth factor receptor overexpression. PH Akademperiodyka 2023-06-01 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2020-1-8 10.32471/exp-oncology.2312-8852.vol-42-no-1.14145 Experimental Oncology; Vol. 42 No. 1 (2020): Experimental Oncology; 25-30 Експериментальна онкологія; Том 42 № 1 (2020): Експериментальна онкологія; 25-30 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-42-no-1 en https://exp-oncology.com.ua/index.php/Exp/article/view/2020-1-8/2020-1-8 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/
institution Experimental Oncology
baseUrl_str
datestamp_date 2023-10-11T16:42:32Z
collection OJS
language English
topic antibodies.
cancer treatment.
HB-EGF
mitogenic activation
receptors
spellingShingle antibodies.
cancer treatment.
HB-EGF
mitogenic activation
receptors
Siromolot, A.A.
Krynina, O.I.
Kolybo, D.V.
Komisarenko, S.V.
Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro
topic_facet antibodies.
cancer treatment.
HB-EGF
mitogenic activation
receptors
antibodies.
cancer treatment.
HB-EGF
mitogenic activation
receptors
format Article
author Siromolot, A.A.
Krynina, O.I.
Kolybo, D.V.
Komisarenko, S.V.
author_facet Siromolot, A.A.
Krynina, O.I.
Kolybo, D.V.
Komisarenko, S.V.
author_sort Siromolot, A.A.
title Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro
title_short Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro
title_full Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro
title_fullStr Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro
title_full_unstemmed Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro
title_sort antiproliferative and apoptotic effects of anti-human hb-egf neutralizing polyclonal antibodies in vitro
title_alt Antiproliferative and apoptotic effects of anti-human HB-EGF neutralizing polyclonal antibodies in vitro
description Summary. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a member of the epidermal growth factor family and has a variety of physiological and pathophysiological functions. Also, HB-EGF plays a pivotal role in progression of different tumors. So, HB-EGF seems to be a target molecule for the treatment of some cancer types. Aim: To obtain HB-EGF neutralizing polyclonal antibodies and test their anti-proliferative properties in vitro. Materials and Methods: Lab rabbits and mice were used for immunization with recombinant HB-EGF. The effect of generated polyclonal antibodies on viability and apoptosis of human epidermoid carcinoma derived A431 cell line was assessed using MTT and Annexin V-propidium iodide assays. Results: Rabbit polyclonal anti-HB-EGF serum could block binding of soluble HB-EGF to epidermal growth factor receptor/human epidermal growth factor receptor. Also, anti-HB-EGF antibodies could bind to surface of A431 cells which express abnormally high levels of membrane bound proHB-EGF and its receptor. It has been shown that immune serum with polyclonal antibodies against HB-EGF was able to block the mitogenic activation of the cells with HB-EGF and cause apoptotic cell death. Conclusion: Inhibition of HB-EGF activity with neutralizing polyclonal antibodies can effectively inhibit mitogenic activation and cause apoptosis of cancer cells with significant epidermal growth factor receptor overexpression.
publisher PH Akademperiodyka
publishDate 2023
url https://exp-oncology.com.ua/index.php/Exp/article/view/2020-1-8
work_keys_str_mv AT siromolotaa antiproliferativeandapoptoticeffectsofantihumanhbegfneutralizingpolyclonalantibodiesinvitro
AT kryninaoi antiproliferativeandapoptoticeffectsofantihumanhbegfneutralizingpolyclonalantibodiesinvitro
AT kolybodv antiproliferativeandapoptoticeffectsofantihumanhbegfneutralizingpolyclonalantibodiesinvitro
AT komisarenkosv antiproliferativeandapoptoticeffectsofantihumanhbegfneutralizingpolyclonalantibodiesinvitro
first_indexed 2025-07-17T12:16:30Z
last_indexed 2025-07-17T12:16:30Z
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