Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression
Summary. Aim: It has been reported that spiroaminopyrimidine derivatives inhibited the growth and proliferation of various cancer cell lines. In the present study, we evaluated cytotoxic and apoptosis-inducing effects of 2,4-diamino-1,3-diazaspiro[5.5]-9-tert-butyl-2, 4-diene-5-carbonitril (9-tBAP)...
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2023
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Experimental Oncology| _version_ | 1856543517002694656 |
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| author | Dehghan Nayeri, M.J. Mahdavi, M. Fazeli, H. Hosseinpour Feizi, M.A. |
| author_facet | Dehghan Nayeri, M.J. Mahdavi, M. Fazeli, H. Hosseinpour Feizi, M.A. |
| author_sort | Dehghan Nayeri, M.J. |
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| datestamp_date | 2023-10-11T16:42:06Z |
| description | Summary. Aim: It has been reported that spiroaminopyrimidine derivatives inhibited the growth and proliferation of various cancer cell lines. In the present study, we evaluated cytotoxic and apoptosis-inducing effects of 2,4-diamino-1,3-diazaspiro[5.5]-9-tert-butyl-2, 4-diene-5-carbonitril (9-tBAP) on NB4 acute promyelocytic leukemia (APL) cells. Materials and Methods: The cells were treated with 10–100 µM of 9-tBAP. Cytotoxic activity of the compound was measured using the MTT assay. Apoptosis was investigated by Hoechst 33258 staining as well as by Annexin V/PI double staining. Results: The compound under study was found to be highly active cell growth inhibitor with IC50 of 30.0 ± 3.5 µM inducing apoptosis in NB4 cells. Cell cycle analysis by flow cytometry showed a time-dependent increase in sub-G1 cell population. Real-time polymerase chain reaction analysis revealed that the treatment with the compound down-regulated the BIRC5 expression in a time-dependent manner. Conclusion: 9-tBAP displayed potent anti-leukemic activity in vitro thus warranting further investigation. |
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| institution | Experimental Oncology |
| language | English |
| last_indexed | 2025-07-17T12:16:38Z |
| publishDate | 2023 |
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| spelling | oai:ojs2.ex.aqua-time.com.ua:article-2152023-10-11T16:42:06Z Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression Dehghan Nayeri, M.J. Mahdavi, M. Fazeli, H. Hosseinpour Feizi, M.A. apoptosis, cell cycle, NB4 cell line, spiroaminopyrimidine apoptosis, cell cycle, NB4 cell line, spiroaminopyrimidine Summary. Aim: It has been reported that spiroaminopyrimidine derivatives inhibited the growth and proliferation of various cancer cell lines. In the present study, we evaluated cytotoxic and apoptosis-inducing effects of 2,4-diamino-1,3-diazaspiro[5.5]-9-tert-butyl-2, 4-diene-5-carbonitril (9-tBAP) on NB4 acute promyelocytic leukemia (APL) cells. Materials and Methods: The cells were treated with 10–100 µM of 9-tBAP. Cytotoxic activity of the compound was measured using the MTT assay. Apoptosis was investigated by Hoechst 33258 staining as well as by Annexin V/PI double staining. Results: The compound under study was found to be highly active cell growth inhibitor with IC50 of 30.0 ± 3.5 µM inducing apoptosis in NB4 cells. Cell cycle analysis by flow cytometry showed a time-dependent increase in sub-G1 cell population. Real-time polymerase chain reaction analysis revealed that the treatment with the compound down-regulated the BIRC5 expression in a time-dependent manner. Conclusion: 9-tBAP displayed potent anti-leukemic activity in vitro thus warranting further investigation. Summary. Aim: It has been reported that spiroaminopyrimidine derivatives inhibited the growth and proliferation of various cancer cell lines. In the present study, we evaluated cytotoxic and apoptosis-inducing effects of 2,4-diamino-1,3-diazaspiro[5.5]-9-tert-butyl-2, 4-diene-5-carbonitril (9-tBAP) on NB4 acute promyelocytic leukemia (APL) cells. Materials and Methods: The cells were treated with 10–100 µM of 9-tBAP. Cytotoxic activity of the compound was measured using the MTT assay. Apoptosis was investigated by Hoechst 33258 staining as well as by Annexin V/PI double staining. Results: The compound under study was found to be highly active cell growth inhibitor with IC50 of 30.0 ± 3.5 µM inducing apoptosis in NB4 cells. Cell cycle analysis by flow cytometry showed a time-dependent increase in sub-G1 cell population. Real-time polymerase chain reaction analysis revealed that the treatment with the compound down-regulated the BIRC5 expression in a time-dependent manner. Conclusion: 9-tBAP displayed potent anti-leukemic activity in vitro thus warranting further investigation. PH Akademperiodyka 2023-06-02 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2019-4-5 10.32471/exp-oncology.2312-8852.vol-41-no-4.13749 Experimental Oncology; Vol. 41 No. 4 (2019): Experimental Oncology; 312-317 Експериментальна онкологія; Том 41 № 4 (2019): Експериментальна онкологія; 312-317 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-41-no-4 en https://exp-oncology.com.ua/index.php/Exp/article/view/2019-4-5/2019-4-5 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/ |
| spellingShingle | apoptosis cell cycle NB4 cell line spiroaminopyrimidine Dehghan Nayeri, M.J. Mahdavi, M. Fazeli, H. Hosseinpour Feizi, M.A. Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression |
| title | Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression |
| title_alt | Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression |
| title_full | Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression |
| title_fullStr | Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression |
| title_full_unstemmed | Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression |
| title_short | Apoptosis-inducing effect of spiroaminopyrimidine analogue in NB4 leukemia cells via down-regulation of BIRC5 expression |
| title_sort | apoptosis-inducing effect of spiroaminopyrimidine analogue in nb4 leukemia cells via down-regulation of birc5 expression |
| topic | apoptosis cell cycle NB4 cell line spiroaminopyrimidine |
| topic_facet | apoptosis cell cycle NB4 cell line spiroaminopyrimidine apoptosis cell cycle NB4 cell line spiroaminopyrimidine |
| url | https://exp-oncology.com.ua/index.php/Exp/article/view/2019-4-5 |
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