Detection of polyamines using immunobiosensor based on zinc oxide nanoparticles
Summary. Aim: To analyze the performance of biosensor based on nanoparticles of zinc oxide for the detection of spermine and spermidine in solution and in cell culture. Materials and Methods: Zinc oxide nanoparticles were used for preparing biosensor containing antibodies to spermine and spermidine....
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PH Akademperiodyka
2023
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oai:ojs2.ex.aqua-time.com.ua:article-2262023-10-11T16:42:06Z Detection of polyamines using immunobiosensor based on zinc oxide nanoparticles Detection of polyamines using immunobiosensor based on zinc oxide nanoparticles Prylutskyi, M.P. Starodub, M.F. biosensors, MCF-7 cells, nanostructures, photoluminescence, spermidine, spermine biosensors, MCF-7 cells, nanostructures, photoluminescence, spermidine, spermine Summary. Aim: To analyze the performance of biosensor based on nanoparticles of zinc oxide for the detection of spermine and spermidine in solution and in cell culture. Materials and Methods: Zinc oxide nanoparticles were used for preparing biosensor containing antibodies to spermine and spermidine. Polyamine concentration is solutions of spermine and spermidine as well as in lyophilisate of MCF-7 cells was measured by luminescence of the samples excited by laser beam at 380 nm. Results: The minimum concentration for the detection of polyamines in model solutions is 10 ng/ml, and maximum one is 100 ng/ml. A higher level of luminescence intensity of nanoparticles was found during analysis the polyamines in MCF-7 lyophilisate allowing for detecting polyamines at concentrations from 100 cells/ml to 100,000 cells/ml. Conclusions: The proposed biosensor system for determining the level of biogenic polyamines in cell lyophilisate using the optical properties of zinc oxide nanoparticles is promising for further improvement of the methodology and its implementation for detection and measurement of polyamines in biological systems. Summary. Aim: To analyze the performance of biosensor based on nanoparticles of zinc oxide for the detection of spermine and spermidine in solution and in cell culture. Materials and Methods: Zinc oxide nanoparticles were used for preparing biosensor containing antibodies to spermine and spermidine. Polyamine concentration is solutions of spermine and spermidine as well as in lyophilisate of MCF-7 cells was measured by luminescence of the samples excited by laser beam at 380 nm. Results: The minimum concentration for the detection of polyamines in model solutions is 10 ng/ml, and maximum one is 100 ng/ml. A higher level of luminescence intensity of nanoparticles was found during analysis the polyamines in MCF-7 lyophilisate allowing for detecting polyamines at concentrations from 100 cells/ml to 100,000 cells/ml. Conclusions: The proposed biosensor system for determining the level of biogenic polyamines in cell lyophilisate using the optical properties of zinc oxide nanoparticles is promising for further improvement of the methodology and its implementation for detection and measurement of polyamines in biological systems. PH Akademperiodyka 2023-06-02 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2019-4-16 10.32471/exp-oncology.2312-8852.vol-41-no-4.13920 Experimental Oncology; Vol. 41 No. 4 (2019): Experimental Oncology; 363-365 Експериментальна онкологія; Том 41 № 4 (2019): Експериментальна онкологія; 363-365 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-41-no-4 en https://exp-oncology.com.ua/index.php/Exp/article/view/2019-4-16/2019-4-16 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/ |
| institution |
Experimental Oncology |
| baseUrl_str |
|
| datestamp_date |
2023-10-11T16:42:06Z |
| collection |
OJS |
| language |
English |
| topic |
biosensors MCF-7 cells nanostructures photoluminescence spermidine spermine |
| spellingShingle |
biosensors MCF-7 cells nanostructures photoluminescence spermidine spermine Prylutskyi, M.P. Starodub, M.F. Detection of polyamines using immunobiosensor based on zinc oxide nanoparticles |
| topic_facet |
biosensors MCF-7 cells nanostructures photoluminescence spermidine spermine biosensors MCF-7 cells nanostructures photoluminescence spermidine spermine |
| format |
Article |
| author |
Prylutskyi, M.P. Starodub, M.F. |
| author_facet |
Prylutskyi, M.P. Starodub, M.F. |
| author_sort |
Prylutskyi, M.P. |
| title |
Detection of polyamines using immunobiosensor based on zinc oxide nanoparticles |
| title_short |
Detection of polyamines using immunobiosensor based on zinc oxide nanoparticles |
| title_full |
Detection of polyamines using immunobiosensor based on zinc oxide nanoparticles |
| title_fullStr |
Detection of polyamines using immunobiosensor based on zinc oxide nanoparticles |
| title_full_unstemmed |
Detection of polyamines using immunobiosensor based on zinc oxide nanoparticles |
| title_sort |
detection of polyamines using immunobiosensor based on zinc oxide nanoparticles |
| title_alt |
Detection of polyamines using immunobiosensor based on zinc oxide nanoparticles |
| description |
Summary. Aim: To analyze the performance of biosensor based on nanoparticles of zinc oxide for the detection of spermine and spermidine in solution and in cell culture. Materials and Methods: Zinc oxide nanoparticles were used for preparing biosensor containing antibodies to spermine and spermidine. Polyamine concentration is solutions of spermine and spermidine as well as in lyophilisate of MCF-7 cells was measured by luminescence of the samples excited by laser beam at 380 nm. Results: The minimum concentration for the detection of polyamines in model solutions is 10 ng/ml, and maximum one is 100 ng/ml. A higher level of luminescence intensity of nanoparticles was found during analysis the polyamines in MCF-7 lyophilisate allowing for detecting polyamines at concentrations from 100 cells/ml to 100,000 cells/ml. Conclusions: The proposed biosensor system for determining the level of biogenic polyamines in cell lyophilisate using the optical properties of zinc oxide nanoparticles is promising for further improvement of the methodology and its implementation for detection and measurement of polyamines in biological systems. |
| publisher |
PH Akademperiodyka |
| publishDate |
2023 |
| url |
https://exp-oncology.com.ua/index.php/Exp/article/view/2019-4-16 |
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AT prylutskyimp detectionofpolyaminesusingimmunobiosensorbasedonzincoxidenanoparticles AT starodubmf detectionofpolyaminesusingimmunobiosensorbasedonzincoxidenanoparticles |
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2025-07-17T12:16:46Z |
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2025-07-17T12:16:46Z |
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