Antitumor effects and hematotoxicity of C60-Cis-Pt nanocomplex in mice with Lewis lung carcinoma

Summary. Background: Cisplatin (Cis-Pt) is a widely used anticancer drug but its therapeutic efficiency is limited by hemato-, cardio-, hepato-, nephro- and neurotoxicity. Complexation of Cis-Pt with C60 fullerene nanoparticle will allow to enhance the antitumor activity of the drug and to...

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Datum:2023
Hauptverfasser: Prylutska, S.V., Lynchak, O.V., Kostjukov, V.V., Evstigneev, M.P., Remeniak, O.V., Rybalchenko, V.K., Prylutskyy, Yu.I., Ritter, U., Scharff, P.
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Sprache:English
Veröffentlicht: PH Akademperiodyka 2023
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Online Zugang:https://exp-oncology.com.ua/index.php/Exp/article/view/2019-2-4
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Experimental Oncology
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institution Experimental Oncology
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datestamp_date 2023-10-11T16:41:24Z
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language English
topic C60 fullerene
cisplatin
hematological indices
Lewis lung carcinoma
tumor-bearing mice survival
С60-Cis-Pt nanocomplex
spellingShingle C60 fullerene
cisplatin
hematological indices
Lewis lung carcinoma
tumor-bearing mice survival
С60-Cis-Pt nanocomplex
Prylutska, S.V.
Lynchak, O.V.
Kostjukov, V.V.
Evstigneev, M.P.
Remeniak, O.V.
Rybalchenko, V.K.
Prylutskyy, Yu.I.
Ritter, U.
Scharff, P.
Antitumor effects and hematotoxicity of C60-Cis-Pt nanocomplex in mice with Lewis lung carcinoma
topic_facet C60 fullerene
cisplatin
hematological indices
Lewis lung carcinoma
tumor-bearing mice survival
С60-Cis-Pt nanocomplex
C60 fullerene
cisplatin
hematological indices
Lewis lung carcinoma
tumor-bearing mice survival
С60-Cis-Pt nanocomplex
format Article
author Prylutska, S.V.
Lynchak, O.V.
Kostjukov, V.V.
Evstigneev, M.P.
Remeniak, O.V.
Rybalchenko, V.K.
Prylutskyy, Yu.I.
Ritter, U.
Scharff, P.
author_facet Prylutska, S.V.
Lynchak, O.V.
Kostjukov, V.V.
Evstigneev, M.P.
Remeniak, O.V.
Rybalchenko, V.K.
Prylutskyy, Yu.I.
Ritter, U.
Scharff, P.
author_sort Prylutska, S.V.
title Antitumor effects and hematotoxicity of C60-Cis-Pt nanocomplex in mice with Lewis lung carcinoma
title_short Antitumor effects and hematotoxicity of C60-Cis-Pt nanocomplex in mice with Lewis lung carcinoma
title_full Antitumor effects and hematotoxicity of C60-Cis-Pt nanocomplex in mice with Lewis lung carcinoma
title_fullStr Antitumor effects and hematotoxicity of C60-Cis-Pt nanocomplex in mice with Lewis lung carcinoma
title_full_unstemmed Antitumor effects and hematotoxicity of C60-Cis-Pt nanocomplex in mice with Lewis lung carcinoma
title_sort antitumor effects and hematotoxicity of c60-cis-pt nanocomplex in mice with lewis lung carcinoma
title_alt Antitumor effects and hematotoxicity of C60-Cis-Pt nanocomplex in mice with Lewis lung carcinoma
description Summary. Background: Cisplatin (Cis-Pt) is a widely used anticancer drug but its therapeutic efficiency is limited by hemato-, cardio-, hepato-, nephro- and neurotoxicity. Complexation of Cis-Pt with C60 fullerene nanoparticle will allow to enhance the antitumor activity of the drug and to reduce its side toxic effects. Aim: To estimate the antitumor effects of С60-Cis-Pt nanocomplex in Lewis lung carcinoma (LLC) and analyze hematological toxicity in tumor-bearing mice. Materials and Methods: Complexation of C60 fullerene and Cis-Pt molecule was studied by computer simulation. С60-Cis-Pt nanocomplex was i.p. injected to LLC-bearing mice in a total dose of 7.5 mg/kg (C60:Cis-Pt as 3.75:3.75 mg/kg). The survival of tumor-bearing mice and the relative reduction of tumor weight was recorded. Blood indices were determined using the Particle Counter PCE­210 automatic hematology analyzer. Results: Computer simulation demonstrated the formation of С60-Cis-Pt nanocomplex in physiological medium and its stability due to the hydrophobic interactions. Treatment with C60-Cis-Pt nanocomplex increased survival time of LLC-bearing mice by 32%, normalized hemoglobin content (up to 100 g/l), erythrocyte and platelet count as compared to the untreated LLC-bearing mice. Tumor weight decreased by 35.5%; the mitotic index of tumor cells decreased by 78%, and apoptotic index increased by 75%. The revealed effects of the C60-Cis-Pt nanocomplex were more pronounced than the effects of Cis-Pt or C60 fullerene alone in equivalent dose. Conclusion: Treatment with C60-Cis-Pt nanocomplex prolonged the survival of LLC-bearing mice and reduced anemia in LLC-bearing mice.
publisher PH Akademperiodyka
publishDate 2023
url https://exp-oncology.com.ua/index.php/Exp/article/view/2019-2-4
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AT kostjukovvv antitumoreffectsandhematotoxicityofc60cisptnanocomplexinmicewithlewislungcarcinoma
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spelling oai:ojs2.ex.aqua-time.com.ua:article-2452023-10-11T16:41:24Z Antitumor effects and hematotoxicity of C60-Cis-Pt nanocomplex in mice with Lewis lung carcinoma Antitumor effects and hematotoxicity of C60-Cis-Pt nanocomplex in mice with Lewis lung carcinoma Prylutska, S.V. Lynchak, O.V. Kostjukov, V.V. Evstigneev, M.P. Remeniak, O.V. Rybalchenko, V.K. Prylutskyy, Yu.I. Ritter, U. Scharff, P. C60 fullerene, cisplatin, hematological indices, Lewis lung carcinoma, tumor-bearing mice survival, С60-Cis-Pt nanocomplex C60 fullerene, cisplatin, hematological indices, Lewis lung carcinoma, tumor-bearing mice survival, С60-Cis-Pt nanocomplex Summary. Background: Cisplatin (Cis-Pt) is a widely used anticancer drug but its therapeutic efficiency is limited by hemato-, cardio-, hepato-, nephro- and neurotoxicity. Complexation of Cis-Pt with C60 fullerene nanoparticle will allow to enhance the antitumor activity of the drug and to reduce its side toxic effects. Aim: To estimate the antitumor effects of С60-Cis-Pt nanocomplex in Lewis lung carcinoma (LLC) and analyze hematological toxicity in tumor-bearing mice. Materials and Methods: Complexation of C60 fullerene and Cis-Pt molecule was studied by computer simulation. С60-Cis-Pt nanocomplex was i.p. injected to LLC-bearing mice in a total dose of 7.5 mg/kg (C60:Cis-Pt as 3.75:3.75 mg/kg). The survival of tumor-bearing mice and the relative reduction of tumor weight was recorded. Blood indices were determined using the Particle Counter PCE­210 automatic hematology analyzer. Results: Computer simulation demonstrated the formation of С60-Cis-Pt nanocomplex in physiological medium and its stability due to the hydrophobic interactions. Treatment with C60-Cis-Pt nanocomplex increased survival time of LLC-bearing mice by 32%, normalized hemoglobin content (up to 100 g/l), erythrocyte and platelet count as compared to the untreated LLC-bearing mice. Tumor weight decreased by 35.5%; the mitotic index of tumor cells decreased by 78%, and apoptotic index increased by 75%. The revealed effects of the C60-Cis-Pt nanocomplex were more pronounced than the effects of Cis-Pt or C60 fullerene alone in equivalent dose. Conclusion: Treatment with C60-Cis-Pt nanocomplex prolonged the survival of LLC-bearing mice and reduced anemia in LLC-bearing mice. Summary. Background: Cisplatin (Cis-Pt) is a widely used anticancer drug but its therapeutic efficiency is limited by hemato-, cardio-, hepato-, nephro- and neurotoxicity. Complexation of Cis-Pt with C60 fullerene nanoparticle will allow to enhance the antitumor activity of the drug and to reduce its side toxic effects. Aim: To estimate the antitumor effects of С60-Cis-Pt nanocomplex in Lewis lung carcinoma (LLC) and analyze hematological toxicity in tumor-bearing mice. Materials and Methods: Complexation of C60 fullerene and Cis-Pt molecule was studied by computer simulation. С60-Cis-Pt nanocomplex was i.p. injected to LLC-bearing mice in a total dose of 7.5 mg/kg (C60:Cis-Pt as 3.75:3.75 mg/kg). The survival of tumor-bearing mice and the relative reduction of tumor weight was recorded. Blood indices were determined using the Particle Counter PCE­210 automatic hematology analyzer. Results: Computer simulation demonstrated the formation of С60-Cis-Pt nanocomplex in physiological medium and its stability due to the hydrophobic interactions. Treatment with C60-Cis-Pt nanocomplex increased survival time of LLC-bearing mice by 32%, normalized hemoglobin content (up to 100 g/l), erythrocyte and platelet count as compared to the untreated LLC-bearing mice. Tumor weight decreased by 35.5%; the mitotic index of tumor cells decreased by 78%, and apoptotic index increased by 75%. The revealed effects of the C60-Cis-Pt nanocomplex were more pronounced than the effects of Cis-Pt or C60 fullerene alone in equivalent dose. Conclusion: Treatment with C60-Cis-Pt nanocomplex prolonged the survival of LLC-bearing mice and reduced anemia in LLC-bearing mice. PH Akademperiodyka 2023-06-05 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2019-2-4 10.32471/exp-oncology.2312-8852.vol-41-no-2.13030 Experimental Oncology; Vol. 41 No. 2 (2019): Experimental Oncology; 106-111 Експериментальна онкологія; Том 41 № 2 (2019): Експериментальна онкологія; 106-111 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-41-no-2 en https://exp-oncology.com.ua/index.php/Exp/article/view/2019-2-4/2019-2-4 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/