Expression profiling of plac1 in murine cancer cell lines
Summary. Aim: Placenta-specific 1 (PLAC1) is among recently-discovered placental antigens which exerts fundamental role in placental function and development. Increasing body of literature shows that PLAC1 is frequently activated and expressed in a wide variety of human cancers and promote cancer pr...
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PH Akademperiodyka
2023
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Experimental Oncology| _version_ | 1856543541535178752 |
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| author | Mahmoudian, J. Ghods, R. Nazari, M. Jeddi-Tehrani, M. Ghahremani, M.H. Ostad, S.N. Zarnani, A.H. |
| author_facet | Mahmoudian, J. Ghods, R. Nazari, M. Jeddi-Tehrani, M. Ghahremani, M.H. Ostad, S.N. Zarnani, A.H. |
| author_sort | Mahmoudian, J. |
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| datestamp_date | 2025-04-30T12:08:14Z |
| description | Summary. Aim: Placenta-specific 1 (PLAC1) is among recently-discovered placental antigens which exerts fundamental role in placental function and development. Increasing body of literature shows that PLAC1 is frequently activated and expressed in a wide variety of human cancers and promote cancer progression. However, no data is available regarding the expression of mouse orthologue, plac1, in murine cancer cell lines. Materials and Methods: We investigated the expression of plac1 in a series of murine cell lines from different histological origins, mammary carcinoma (4T1), melanoma (B16F10), colorectal carcinoma (CT26), renal carcinoma (Renca), glioma (GL26), B-cell lymphoma (A20 and BCL1) and also two fibroblast cell lines (NIH3T3 and L929), using RT-PCR, Western blotting and flow cytometry. Results: Our data demonstrated that plac1 transcript and plac1 protein were expressed in all examined cell lines, as judged by RT-PCR and Western blot, respectively. The molecular weight of mouse plac1 was experimentally observed to be approximately 24 kD. Flow cytometric analysis showed surface expression of plac1 in aforesaid cell lines ranging from 2% to 42.5%. Conclusion: Based on the ubiquitous expression of plac1, the investigated cancer cell lines or immortalized cell lines can be used to examine the role of plac1 in the process of immortalization. |
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| institution | Experimental Oncology |
| language | English |
| last_indexed | 2025-07-17T12:17:12Z |
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| spelling | oai:ojs2.ex.aqua-time.com.ua:article-2642025-04-30T12:08:14Z Expression profiling of plac1 in murine cancer cell lines Expression profiling of plac1 in murine cancer cell lines Mahmoudian, J. Ghods, R. Nazari, M. Jeddi-Tehrani, M. Ghahremani, M.H. Ostad, S.N. Zarnani, A.H. cancer, cell line, expression, immortalization, plac1 cancer, cell line, expression, immortalization, plac1 Summary. Aim: Placenta-specific 1 (PLAC1) is among recently-discovered placental antigens which exerts fundamental role in placental function and development. Increasing body of literature shows that PLAC1 is frequently activated and expressed in a wide variety of human cancers and promote cancer progression. However, no data is available regarding the expression of mouse orthologue, plac1, in murine cancer cell lines. Materials and Methods: We investigated the expression of plac1 in a series of murine cell lines from different histological origins, mammary carcinoma (4T1), melanoma (B16F10), colorectal carcinoma (CT26), renal carcinoma (Renca), glioma (GL26), B-cell lymphoma (A20 and BCL1) and also two fibroblast cell lines (NIH3T3 and L929), using RT-PCR, Western blotting and flow cytometry. Results: Our data demonstrated that plac1 transcript and plac1 protein were expressed in all examined cell lines, as judged by RT-PCR and Western blot, respectively. The molecular weight of mouse plac1 was experimentally observed to be approximately 24 kD. Flow cytometric analysis showed surface expression of plac1 in aforesaid cell lines ranging from 2% to 42.5%. Conclusion: Based on the ubiquitous expression of plac1, the investigated cancer cell lines or immortalized cell lines can be used to examine the role of plac1 in the process of immortalization. Summary. Aim: Placenta-specific 1 (PLAC1) is among recently-discovered placental antigens which exerts fundamental role in placental function and development. Increasing body of literature shows that PLAC1 is frequently activated and expressed in a wide variety of human cancers and promote cancer progression. However, no data is available regarding the expression of mouse orthologue, plac1, in murine cancer cell lines. Materials and Methods: We investigated the expression of plac1 in a series of murine cell lines from different histological origins, mammary carcinoma (4T1), melanoma (B16F10), colorectal carcinoma (CT26), renal carcinoma (Renca), glioma (GL26), B-cell lymphoma (A20 and BCL1) and also two fibroblast cell lines (NIH3T3 and L929), using RT-PCR, Western blotting and flow cytometry. Results: Our data demonstrated that plac1 transcript and plac1 protein were expressed in all examined cell lines, as judged by RT-PCR and Western blot, respectively. The molecular weight of mouse plac1 was experimentally observed to be approximately 24 kD. Flow cytometric analysis showed surface expression of plac1 in aforesaid cell lines ranging from 2% to 42.5%. Conclusion: Based on the ubiquitous expression of plac1, the investigated cancer cell lines or immortalized cell lines can be used to examine the role of plac1 in the process of immortalization. PH Akademperiodyka 2023-06-08 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2019-1-6 10.32471/exp-oncology.2312-8852.vol-41-no-1.12743 Experimental Oncology; Vol. 41 No. 1 (2019): Experimental Oncology; 7-13 Експериментальна онкологія; Том 41 № 1 (2019): Експериментальна онкологія; 7-13 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-41-no-1 en https://exp-oncology.com.ua/index.php/Exp/article/view/2019-1-6/2019-1-6 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/ |
| spellingShingle | cancer cell line expression immortalization plac1 Mahmoudian, J. Ghods, R. Nazari, M. Jeddi-Tehrani, M. Ghahremani, M.H. Ostad, S.N. Zarnani, A.H. Expression profiling of plac1 in murine cancer cell lines |
| title | Expression profiling of plac1 in murine cancer cell lines |
| title_alt | Expression profiling of plac1 in murine cancer cell lines |
| title_full | Expression profiling of plac1 in murine cancer cell lines |
| title_fullStr | Expression profiling of plac1 in murine cancer cell lines |
| title_full_unstemmed | Expression profiling of plac1 in murine cancer cell lines |
| title_short | Expression profiling of plac1 in murine cancer cell lines |
| title_sort | expression profiling of plac1 in murine cancer cell lines |
| topic | cancer cell line expression immortalization plac1 |
| topic_facet | cancer cell line expression immortalization plac1 cancer cell line expression immortalization plac1 |
| url | https://exp-oncology.com.ua/index.php/Exp/article/view/2019-1-6 |
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