DNA oxidation in patients with metastastic colorectal cancer: clinical significance of 8-hydroxy-deoxyguanosine as an independent prognostic factor
Summary. Prognosis of metastatic colorectal cancer (mCRC) patients nowadays is an important subject in the field of oncology. R0-resection of colon with primary tumor and liver metastasis remains the only treatment which significantly improves survival rate. However, recent experimental data show th...
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2023
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Experimental Oncology |
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2025-04-30T12:08:14Z |
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English |
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8-hydroxy-deoxyguanosine colorectal cancer. liver resection metastases superoxide radicals |
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8-hydroxy-deoxyguanosine colorectal cancer. liver resection metastases superoxide radicals Burlaka, A.A. Vovk, A.V. Burlaka, A.P. Kolesnik, O.O. DNA oxidation in patients with metastastic colorectal cancer: clinical significance of 8-hydroxy-deoxyguanosine as an independent prognostic factor |
| topic_facet |
8-hydroxy-deoxyguanosine colorectal cancer. liver resection metastases superoxide radicals 8-hydroxy-deoxyguanosine colorectal cancer. liver resection metastases superoxide radicals |
| format |
Article |
| author |
Burlaka, A.A. Vovk, A.V. Burlaka, A.P. Kolesnik, O.O. |
| author_facet |
Burlaka, A.A. Vovk, A.V. Burlaka, A.P. Kolesnik, O.O. |
| author_sort |
Burlaka, A.A. |
| title |
DNA oxidation in patients with metastastic colorectal cancer: clinical significance of 8-hydroxy-deoxyguanosine as an independent prognostic factor |
| title_short |
DNA oxidation in patients with metastastic colorectal cancer: clinical significance of 8-hydroxy-deoxyguanosine as an independent prognostic factor |
| title_full |
DNA oxidation in patients with metastastic colorectal cancer: clinical significance of 8-hydroxy-deoxyguanosine as an independent prognostic factor |
| title_fullStr |
DNA oxidation in patients with metastastic colorectal cancer: clinical significance of 8-hydroxy-deoxyguanosine as an independent prognostic factor |
| title_full_unstemmed |
DNA oxidation in patients with metastastic colorectal cancer: clinical significance of 8-hydroxy-deoxyguanosine as an independent prognostic factor |
| title_sort |
dna oxidation in patients with metastastic colorectal cancer: clinical significance of 8-hydroxy-deoxyguanosine as an independent prognostic factor |
| title_alt |
DNA oxidation in patients with metastastic colorectal cancer: clinical significance of 8-hydroxy-deoxyguanosine as an independent prognostic factor |
| description |
Summary. Prognosis of metastatic colorectal cancer (mCRC) patients nowadays is an important subject in the field of oncology. R0-resection of colon with primary tumor and liver metastasis remains the only treatment which significantly improves survival rate. However, recent experimental data show that surgical trauma can indirectly stimulate tumor growth due to mitochondrial dysfunction and unregulated superoxide radical (O2-) generation. Aim: To study the clinical significance of 8-oxo-2'-deoxyguanosine (8-oxodGu) marker, to assess the oncological effects of warm ischemia of liver parenchyma on disease prognosis in patients with mCRC. Material and Methods: 69 urine 24-hour volume tests of patients with mCRC and 17 healthy individuals were studied. Urine 8-oxodGu level was measured using spectrophotometric method with pre-solid phase DNA extraction. The energy system and hepatocyte detoxification system state, levels of O2- in tumor tissue were determined using the method of electron paramagnetic resonance (EPR) and SpinTraps technology at room temperature. Experiments were carried out on a computerized EPR spectrometer RE-1307. EPR spectra were recorded at temperature of liquid nitrogen (–196 °C) in paramagnetically pure quartz dewar on a computerized spectrometer PE-1307 with resonator H011. Error of the method of spectrum integration and spread of spectrum reproduction of one sample was not more than 3%. Results: The average level of marker in healthy individuals was 0.244 ± 0.063 nM/kg ∙ day, whereas before the resection and on day 3 after the R0-resection of liver in mCRC patients was 3.42 ± 0.18 nM/kg ∙ day and 2.12 ± 0.08 nM/kg ∙ day (p < 0.05), respectively. On day 3 after the liver resection due to its metastatic lesions with a total duration of warm ischemia period < 30 min and > 30 min have had marker at level 2.108 ± 0.13 nM/kg ∙ day and 2.9883 ± 0.159 nM/kg ∙ day (p < 0.0001), respectively. The volume of metastatic tissue significantly and directly correlated with the level of urine 8-oxodGu (R2 = 0.54, 95% CI 0.037–0.0991, p < 0.0001), also duration of surgical intervention (≥ 300 min) and duration of worm liver ischemia (> 30 min) during the surgery significantly increased urine level of 8-oxodGu (R2 = 0.54, 95% CI 0.001–0.004, p < 0.001). Conclusions: Warm liver ischemia (> 30 min), long-term surgical intervention (≥ 300 min) and metastatic tissue volume (≥ 12 cm3) in liver parenchyma in mCRC patients significantly increase urine 8-oxodGu levels. R0-resection of liver metastases in mCRC patients decreases urine 8-oxodGu levels already on day 3 after the surgery. 8-oxodGu is a new factor of oncological prognosis in patients with mCRC. |
| publisher |
PH Akademperiodyka |
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2023 |
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https://exp-oncology.com.ua/index.php/Exp/article/view/2019-1-10 |
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oai:ojs2.ex.aqua-time.com.ua:article-2682025-04-30T12:08:14Z DNA oxidation in patients with metastastic colorectal cancer: clinical significance of 8-hydroxy-deoxyguanosine as an independent prognostic factor DNA oxidation in patients with metastastic colorectal cancer: clinical significance of 8-hydroxy-deoxyguanosine as an independent prognostic factor Burlaka, A.A. Vovk, A.V. Burlaka, A.P. Kolesnik, O.O. 8-hydroxy-deoxyguanosine, colorectal cancer., liver resection, metastases, superoxide radicals 8-hydroxy-deoxyguanosine, colorectal cancer., liver resection, metastases, superoxide radicals Summary. Prognosis of metastatic colorectal cancer (mCRC) patients nowadays is an important subject in the field of oncology. R0-resection of colon with primary tumor and liver metastasis remains the only treatment which significantly improves survival rate. However, recent experimental data show that surgical trauma can indirectly stimulate tumor growth due to mitochondrial dysfunction and unregulated superoxide radical (O2-) generation. Aim: To study the clinical significance of 8-oxo-2'-deoxyguanosine (8-oxodGu) marker, to assess the oncological effects of warm ischemia of liver parenchyma on disease prognosis in patients with mCRC. Material and Methods: 69 urine 24-hour volume tests of patients with mCRC and 17 healthy individuals were studied. Urine 8-oxodGu level was measured using spectrophotometric method with pre-solid phase DNA extraction. The energy system and hepatocyte detoxification system state, levels of O2- in tumor tissue were determined using the method of electron paramagnetic resonance (EPR) and SpinTraps technology at room temperature. Experiments were carried out on a computerized EPR spectrometer RE-1307. EPR spectra were recorded at temperature of liquid nitrogen (–196 °C) in paramagnetically pure quartz dewar on a computerized spectrometer PE-1307 with resonator H011. Error of the method of spectrum integration and spread of spectrum reproduction of one sample was not more than 3%. Results: The average level of marker in healthy individuals was 0.244 ± 0.063 nM/kg ∙ day, whereas before the resection and on day 3 after the R0-resection of liver in mCRC patients was 3.42 ± 0.18 nM/kg ∙ day and 2.12 ± 0.08 nM/kg ∙ day (p < 0.05), respectively. On day 3 after the liver resection due to its metastatic lesions with a total duration of warm ischemia period < 30 min and > 30 min have had marker at level 2.108 ± 0.13 nM/kg ∙ day and 2.9883 ± 0.159 nM/kg ∙ day (p < 0.0001), respectively. The volume of metastatic tissue significantly and directly correlated with the level of urine 8-oxodGu (R2 = 0.54, 95% CI 0.037–0.0991, p < 0.0001), also duration of surgical intervention (≥ 300 min) and duration of worm liver ischemia (> 30 min) during the surgery significantly increased urine level of 8-oxodGu (R2 = 0.54, 95% CI 0.001–0.004, p < 0.001). Conclusions: Warm liver ischemia (> 30 min), long-term surgical intervention (≥ 300 min) and metastatic tissue volume (≥ 12 cm3) in liver parenchyma in mCRC patients significantly increase urine 8-oxodGu levels. R0-resection of liver metastases in mCRC patients decreases urine 8-oxodGu levels already on day 3 after the surgery. 8-oxodGu is a new factor of oncological prognosis in patients with mCRC. Summary. Prognosis of metastatic colorectal cancer (mCRC) patients nowadays is an important subject in the field of oncology. R0-resection of colon with primary tumor and liver metastasis remains the only treatment which significantly improves survival rate. However, recent experimental data show that surgical trauma can indirectly stimulate tumor growth due to mitochondrial dysfunction and unregulated superoxide radical (O2-) generation. Aim: To study the clinical significance of 8-oxo-2'-deoxyguanosine (8-oxodGu) marker, to assess the oncological effects of warm ischemia of liver parenchyma on disease prognosis in patients with mCRC. Material and Methods: 69 urine 24-hour volume tests of patients with mCRC and 17 healthy individuals were studied. Urine 8-oxodGu level was measured using spectrophotometric method with pre-solid phase DNA extraction. The energy system and hepatocyte detoxification system state, levels of O2- in tumor tissue were determined using the method of electron paramagnetic resonance (EPR) and SpinTraps technology at room temperature. Experiments were carried out on a computerized EPR spectrometer RE-1307. EPR spectra were recorded at temperature of liquid nitrogen (–196 °C) in paramagnetically pure quartz dewar on a computerized spectrometer PE-1307 with resonator H011. Error of the method of spectrum integration and spread of spectrum reproduction of one sample was not more than 3%. Results: The average level of marker in healthy individuals was 0.244 ± 0.063 nM/kg ∙ day, whereas before the resection and on day 3 after the R0-resection of liver in mCRC patients was 3.42 ± 0.18 nM/kg ∙ day and 2.12 ± 0.08 nM/kg ∙ day (p < 0.05), respectively. On day 3 after the liver resection due to its metastatic lesions with a total duration of warm ischemia period < 30 min and > 30 min have had marker at level 2.108 ± 0.13 nM/kg ∙ day and 2.9883 ± 0.159 nM/kg ∙ day (p < 0.0001), respectively. The volume of metastatic tissue significantly and directly correlated with the level of urine 8-oxodGu (R2 = 0.54, 95% CI 0.037–0.0991, p < 0.0001), also duration of surgical intervention (≥ 300 min) and duration of worm liver ischemia (> 30 min) during the surgery significantly increased urine level of 8-oxodGu (R2 = 0.54, 95% CI 0.001–0.004, p < 0.001). Conclusions: Warm liver ischemia (> 30 min), long-term surgical intervention (≥ 300 min) and metastatic tissue volume (≥ 12 cm3) in liver parenchyma in mCRC patients significantly increase urine 8-oxodGu levels. R0-resection of liver metastases in mCRC patients decreases urine 8-oxodGu levels already on day 3 after the surgery. 8-oxodGu is a new factor of oncological prognosis in patients with mCRC. PH Akademperiodyka 2023-06-08 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/2019-1-10 10.32471/exp-oncology.2312-8852.vol-41-no-1.12554 Experimental Oncology; Vol. 41 No. 1 (2019): Experimental Oncology; 26-31 Експериментальна онкологія; Том 41 № 1 (2019): Експериментальна онкологія; 26-31 2312-8852 1812-9269 10.32471/exp-oncology.2312-8852.vol-41-no-1 en https://exp-oncology.com.ua/index.php/Exp/article/view/2019-1-10/2019-1-10 Copyright (c) 2023 Experimental Oncology https://creativecommons.org/licenses/by-nc/4.0/ |