ПРОТИПУХЛИННА АКТИВНІСТЬ НАНОЧАСТИНОК ДЕКСТРАН-ПОЛІАКРИЛАМІДу/ZnO щодО ЛІНІЙ КЛІТИН РАКУ ПЕРЕДМІХУРОВОЇ ЗАЛОЗИ in vitro

• Datum: 2023
• Hauptverfasser: Virych, P.A., Zadvornyi, T.V., Borikun, T.V., Lykhova, O.O., Chumachenko, V.A., Pavlenko, V.A., Kutsevol, N.V., Lukianova, N.Yu.
• Format: Artikel
• Sprache: English
• Veröffentlicht: PH Akademperiodyka 2023
• Schlagworte: апоптоз; наночастинки; оксид цинку; рак передміхурової залози
• Online Zugang: https://exp-oncology.com.ua/index.php/Exp/article/view/2022-3-8
• Назва журналу: Experimental Oncology

Background: The combination of zinc oxide (ZnO) nanoparticles (NPs) with carriers enhances the anticancer effect of nanocomposites. Aim: To explore the mechanisms of cytotoxic action of dextran-graft-polyacrylamide (D-g-PAA/ZnO) NPs against prostate cancers cell lines in vitro. Materials and Methods: Dextran-polyacrylamide was used as a matrix for the synthesis of ZnO NPs. Prostate cancer cells LNCaP, DU-145 and PC-3 were treated with D-g-PAA/ZnO NPs. The expression of Bax, Bcl-2, p53 and Ki-67 was studied using immunocytochemical analysis. Cytomorphological changes in cells were detected after their incubation with nanocomposites for 24 h. Results: The treatment with D-g-PAA/ZnO NPs caused the increase in the Bax and p53 and the decrease in Ki-67 and Bcl-2 expression. Morphological changes associated with apoptosis were registered: decrease in cell size, appearance of cytoplasmic vacuolation, condensation of chromatin, blebbing. Conclusions: Treatment with D-g-PAA/ZnO nanocomposite led to the initiation of apoptotic cell death in prostate cancer cells in vitro.