ПРОТИПУХЛИННА АКТИВНІСТЬ НАНОЧАСТИНОК ДЕКСТРАН-ПОЛІАКРИЛАМІДу/ZnO щодО ЛІНІЙ КЛІТИН РАКУ ПЕРЕДМІХУРОВОЇ ЗАЛОЗИ in vitro
Background: The combination of zinc oxide (ZnO) nanoparticles (NPs) with carriers enhances the anticancer effect of nanocomposites. Aim: To explore the mechanisms of cytotoxic action of dextran-graft-polyacrylamide (D-g-PAA/ZnO) NPs against prostate cancers cell lines in v...
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| Date: | 2023 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
PH Akademperiodyka
2023
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| Subjects: | |
| Online Access: | https://exp-oncology.com.ua/index.php/Exp/article/view/2022-3-8 |
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| Journal Title: | Experimental Oncology |
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Experimental Oncology| Summary: | Background: The combination of zinc oxide (ZnO) nanoparticles (NPs) with carriers enhances the anticancer effect of nanocomposites. Aim: To explore the mechanisms of cytotoxic action of dextran-graft-polyacrylamide (D-g-PAA/ZnO) NPs against prostate cancers cell lines in vitro. Materials and Methods: Dextran-polyacrylamide was used as a matrix for the synthesis of ZnO NPs. Prostate cancer cells LNCaP, DU-145 and PC-3 were treated with D-g-PAA/ZnO NPs. The expression of Bax, Bcl-2, p53 and Ki-67 was studied using immunocytochemical analysis. Cytomorphological changes in cells were detected after their incubation with nanocomposites for 24 h. Results: The treatment with D-g-PAA/ZnO NPs caused the increase in the Bax and p53 and the decrease in Ki-67 and Bcl-2 expression. Morphological changes associated with apoptosis were registered: decrease in cell size, appearance of cytoplasmic vacuolation, condensation of chromatin, blebbing. Conclusions: Treatment with D-g-PAA/ZnO nanocomposite led to the initiation of apoptotic cell death in prostate cancer cells in vitro. |
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