ПОРІВНЯННЯ 2DІ 3D ПЕРВИННОЇ КУЛЬТУРИ КЛІТИН, ОТРИМАНОЇ З ЕКСПЛАНТАТА НИЗЬКОДИФЕРЕНЦІЙОВАНОГО УРОТЕЛІАЛЬНОГО РАКУ СЕЧОВОГО МІХУРА

• Datum: 2023
• Hauptverfasser: Garmanchuk, L.V., Yakovlev, P.G., Ostrovska, G.V., Stupak, Yu.A., Skachkova, O.V., Gorbach, O.I., Stepanov, Yu.V.
• Format: Artikel
• Sprache: English
• Veröffentlicht: PH Akademperiodyka 2023
• Schlagworte: рак сечового міхура; первинна культура клітин; сфероїди; лактатдегідрогеназа; апоптоз
• Online Zugang: https://exp-oncology.com.ua/index.php/Exp/article/view/2023-1-14
• Назва журналу: Experimental Oncology

Studying the biological characteristics of bladder cancer in primary culture can be an effective way for diagnostic and prognostic purposes, as well as choosing a scheme for personalized therapy. Aim: To characterize and compare 2D and 3D primary cell cultures obtained from the same tumor sample resected from a patient with high-grade bladder cancer. Materials and Methods: 2D and 3D primary cell cultures were obtained from explants of resected bladder cancer. Glucose metabolism, lactate dehydrogenase (LDH) activity, and level of apoptosis were studied. Results: Multicellular tumor spheroids (3D) differ from planar culture (2D) by more pronounced consumption of glucose from the culture medium (1.7 times higher than 2D on Day 3 of culture), increased lactate dehydrogenase activity (2.5 times higher on Day 3 vs. Day 1 of cultivation, while in 2D culture LDH activity is constant), stronger acidification of the extracellular environment (pH dropped by 1 in 3D and by 0.5 in 2D). Spheroids demonstrate enhanced resistance to apoptosis (1.4 times higher). Conclusion: This methodological technique can be used both for tumor characterization and for selection of optimal postoperative chemotherapeutic schemes.