АСОЦІАЦІЯ ЕКСПРЕСІЇ МІКРОРНК ІЗ ВПЛ СТАТУСОМ У ЗРАЗКАХ ДИСПЛАЗІЇ ШИЙКИ МАТКИ: ПІЛОТНЕ ДОСЛІДЖЕННЯ

• Дата: 2024
• Автори: SVINTSITSKA, A., LYGYRDA, N., Svintsitskyi, V., Borikun, T., Lukianova, N.
• Формат: Стаття
• Мова: English
• Опубліковано: PH Akademperiodyka 2024
• Теми: рак шийки матки; дисплазія шийки матки; ВПЛ; мікроРНК
• Онлайн доступ: https://exp-oncology.com.ua/index.php/Exp/article/view/437
• Назва журналу: Experimental Oncology

Background. Cervical cancer is a major health concern, with human papillomaviruses (HPV) infection being a key risk factor. However, not all HPV-infected individuals develop cancer, suggesting the additional factors may be involved. This study aims to evaluate the differences in the miR-155 and -205 expression in cervical tissue with dysplasia depending on the presence of HPV and confirmed cancer diagnosis. Materials and Methods. The expression of miR-155 and -205 in 30 formalin-fixed paraffin-embedded primary cervical tissue biopsy samples was evaluated using RT-PCR. Results. The expression levels of miRNA-155 and -205 in cervical dysplasia samples without malignant transformation was lower than these in carcinoma in situ tissues (0.74 ± 0.21 and 1.65 ± 0.42 vs. 1.37 ± 0.18 and 2.35 ± 0.32, respectively). In carcinoma in situ cases, we found higher levels of miRNA-155 and -205 (1.6 and 1.38 times, respectively) in CIN-3/ HSIL samples compared to CIN-2/HSIL samples. The expression of both miRNAs tended to increase in HPV-positive cases and in the presence of malignant transformation compared to HPV-negative dysplasia and dysplasia without signs of malignant transformation, respectively. Conclusions. The obtained data indicate a potential relationship between the presence of HPV infection and the expression profile of miRNA-155 and -205.