ДИНАМІЧНИЙ ІНДЕКС АНДРОГЕННОЇ ВІДПОВІДІ ТА ЙОГО АСОЦІАЦІЯ З БІОХІМІЧНИМ РЕЦИДИВОМ ПІСЛЯ РАДИКАЛЬНОЇ ПРОСТАТЕКТОМІЇ НА ТЛІ НЕОАД‘ЮВАНТНОЇ АНДРОГЕН-ДЕПРИВАЦІЙНОЇ ТЕРАПІЇ

Background. Neoadjuvant androgen deprivation therapy (NADT) before radical prostatectomy (RP) remains controversial in the management of localized and locally advanced prostate cancer. A major limitation of this strategy is the lack of biomarkers capable of identifying tumors biologically sensitive...

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Veröffentlicht in:Експериментальна онкологія
Datum:2026
Jahrgang:48
Heft:2
Сторінки:161-171
ISSN:2312-8852
Автори та афіліації:
  • Ye. Afanasiev — O.F. Vozianov Institute of Urology, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
  • S. Vozianov — O.F. Vozianov Institute of Urology, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
  • R. Danylets — O.F. Vozianov Institute of Urology, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
  • V. Grygorenko — O.F. Vozianov Institute of Urology, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
  • O. Shulyak — O.F. Vozianov Institute of Urology, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
  • M. Sosnin — O.F. Vozianov Institute of Urology, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
  • I. Kholokharenko — Department of Urology, Shupyk National Healthcare University of Ukraine, Kyiv, Ukraine
Hauptverfasser: Afanasiev, Ye., Vozianov, S., Danylets, R., Grygorenko, V., Shulyak, O., Sosnin, M., Kholokharenko, I.
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Experimental Oncology
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author Afanasiev, Ye.
Vozianov, S.
Danylets, R.
Grygorenko, V.
Shulyak, O.
Sosnin, M.
Kholokharenko, I.
author_facet Afanasiev, Ye.
Vozianov, S.
Danylets, R.
Grygorenko, V.
Shulyak, O.
Sosnin, M.
Kholokharenko, I.
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description Background. Neoadjuvant androgen deprivation therapy (NADT) before radical prostatectomy (RP) remains controversial in the management of localized and locally advanced prostate cancer. A major limitation of this strategy is the lack of biomarkers capable of identifying tumors biologically sensitive to androgen deprivation. This study aimed to evaluate the prognostic value of a dynamic androgen response index (IAR) and develop a predictive model for biochemical recurrence (BCR) after RP. Materials and Methods. The retrospective single-center study included 84 patients with prostate cancer treated with NADT followed by RP. The IAR was defined as the ratio between logarithmic slopes of PSA and testosterone decline during neoadjuvant therapy. Cox regression analysis was performed to identify predictors of BCR. A predictive model and nomogram were constructed based on independent predictors. Model performance was evaluated using ROC analysis, calibration plots, and decision curve analysis. Results. During a median follow-up of 56 months, BCR occurred in 62 patients. Clinical stage ≥ cT3a (HR 2.34; p = 0.003) and IAR (HR 1.48; p = 0.024) were independent predictors of BCR. The model demonstrated moderate discrimination (AUC 0.68) and satisfactory calibration. Conclusions. The IAR may reflect the biological responsiveness of prostate cancer to androgen deprivation. These findings should be considered hypothesis-generating and require further validation in the context of risk stratification for BCR following RP after NADT.
doi_str_mv 10.15407/exp-oncology.2026.02.161
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fulltext ISSN 1812-9269. Experimental Oncology 48 (2). 2026 161 ORIGINAL CONTRIBUTION C i t a t i o n: Afanasiev Ye, Vozianov S, Danylets R, Grygorenko V, Shulyak O, Sosnin M, Kholokharenko I. Dynamic andro- gen response index and its association with biochemical recurrence after radical prostatectomy following neoadjuvant an- drogen deprivation therapy. Exp Oncol. 2026; 48(2): 161-171. https://doi.org/10.15407/exp-oncology.2026.02.161 © PH “Akademperiodyka” of the NAS of Ukraine, 2026. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/) https://doi.org/10.15407/exp-oncology.2026.02.161 Ye. Afanasiev 1, 2, *, S. Vozianov 1, 2, R. Danylets 1, 2, V. Grygorenko 1, O. Shulyak 1, M. Sosnin 1, 2, I. Kholokharenko 2 1 O.F. Vozianov Institute of Urology, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine 2 Department of Urology, Shupyk National Healthcare University of Ukraine, Kyiv, Ukraine * Correspondence: E-mail: evhenii.afanasev@gmail.com DYNAMIC ANDROGEN RESPONSE INDEX AND ITS ASSOCIATION WITH BIOCHEMICAL RECURRENCE AFTER RADICAL PROSTATECTOMY FOLLOWING NEOADJUVANT ANDROGEN DEPRIVATION THERAPY Background. Neoadjuvant androgen deprivation therapy (NADT) before radical prostatectomy (RP) remains controver- sial in the management of localized and locally advanced prostate cancer. A major limitation of this strategy is the lack of biomarkers capable of identifying tumors biologically sensitive to androgen deprivation. This study aimed to evaluate the prognostic value of a dynamic androgen response index (IAR) and develop a predictive model for biochemical recurrence (BCR) after RP. Materials and Methods. The retrospective single-center study included 84 patients with prostate cancer treated with NADT followed by RP. The IAR was defined as the ratio between logarithmic slopes of PSA and testosterone decline during neoadjuvant therapy. Cox regression analysis was performed to identify predictors of BCR. A predictive model and nomogram were constructed based on independent predictors. Model performance was evaluated using ROC analysis, calibration plots, and decision curve analysis. Results. During a median follow-up of 56 months, BCR occurred in 62 patients. Clinical stage ≥ cT3a (HR 2.34; p = 0.003) and IAR (HR 1.48; p = 0.024) were independent predictors of BCR. The model demonstrated moderate discrimination (AUC 0.68) and satisfactory calibration. Conclusions. The IAR may reflect the biological responsiveness of prostate cancer to androgen deprivation. These findings should be considered hypothesis-generating and require further validation in the context of risk stratification for BCR following RP after NADT. Keywords: prostate cancer, neoadjuvant androgen deprivation therapy, biochemical recurrence, predictive model, no- mogram, androgen response. Prostate cancer (PCa) remains one of the most commonly diagnosed malignancies among men worldwide [1]. While radical prostatectomy (RP) represents an established treatment option for pa- tients with low and intermediate risk [2], oncolog- ical outcomes remain suboptimal in men with high https://doi.org/10.15407/exp-oncology.2026.02.161 https://creativecommons.org/licenses/by-nc-nd/4.0/ https://doi.org/10.15407/exp-oncology.2026.02 mailto:evhenii.afanasev@gmail.com 162 ISSN 1812-9269. Experimental Oncology 48 (2). 2026 Ye. Afanasiev, S. Vozianov, R. Danylets, V. Grygorenko, O. Shulyak, M. Sosnin, I. Kholokharenko and very high risk features, frequently necessitating multimodal treatment strategies [3, 4]. Neoadjuvant androgen deprivation therapy (NADT) before RP has been investigated for se veral decades as a strategy to improve oncological outcomes in patients with localized and locally ad- vanced PCa. Randomized studies have demonstrat- ed that NADT may reduce tumor volume and the rate of positive surgical margins; however, its effect on long-term oncological outcomes such as bio- chemical recurrence (BCR) and overall survival (OS) remains uncertain [5—7]. One of the major limitations of this therapeutic approach is the lack of reliable biomarkers capable of identifying pa- tients who are biologically sensitive to androgen deprivation and therefore most likely to benefit from NADT. Traditionally, the response to androgen depri- vation therapy (ADT) has been evaluated using absolute prostate-specific antigen (PSA) values, including baseline PSA, post-treatment PSA, and PSA nadir [8, 9]. While these parameters are widely used in clinical practice, they represent static measurements that may be strongly influ- enced by initial tumor burden and inter-indivi dual variability. Consequently, absolute PSA val- ues alone may provide limited insight into the bio logical dynamics of tumor response to ADT and may not accurately reflect intrinsic sensitivity of PCa to androgen deprivation. To overcome this limitation, increasing atten- tion has been directed toward the evaluation of PSA kinetics during hormonal therapy. Parame- ters such as the rate of PSA decline, PSA half-life, or logarithmic slopes of PSA change have been proposed as more informative indicators of treat- ment response [10—12]. These dynamic measures reflect the rate of biomarker changes compared to static PSA measurements. Nevertheless, PSA ki- netics alone cannot fully distinguish whether PSA decline results from effective androgen suppres- sion or from intrinsic tumor sensitivity to andro- gen deprivation. Since the principal mechanism of NADT is the suppression of testosterone production, the rela- tionship between PSA dynamics and testosterone decline may more accurately reflect the biological responsiveness of PCa to androgen deprivation. In- tegrating the dynamics of these two biomarkers may therefore provide a more comprehensive as- sessment of treatment response. Based on this con- cept, we hypothesized that integrating the dynam- ics of PSA and testosterone decline may provide a more accurate representation of tumor sensitivity to androgen deprivation. Therefore, we set forward a dynamic androgen response index (IAR), defined as the ratio between the slopes of PSA and testos- terone decline during NADT. The aim of the present study was to evaluate the prognostic significance of IAR and to develop a predictive model for biochemical recurrence in pa- tients undergoing NADT before RP. Materials and Methods Study population. This retrospective single-center study was conducted in the O.F. Vozianov Institute of Urology, the National Academy of Medical Sci- ences of Ukraine (IU NAMS) and included patients with clinically localized and locally advanced PCa who underwent NADT followed by RP at a tertiary referral center. Clinical and pathological data were obtained from institutional medical records bet ween January 2015 and December 2021. Patients were eligible for inclusion if they had histologically confirmed PCa with a clinical stage ≤ cT4 and no evidence of pelvic wall or urethral sphincter invasion on pretreatment magnetic reso- nance imaging performed before the initiation of any systemic or local therapy. Availability of base- line clinical data, including measurements of PSA and serum testosterone levels both before initiation of NADT and completion of neoadjuvant treat- ment, biopsy ISUP, as well as long-term oncological outcomes (date of BCR), was required. Patients with oligometastatic disease at diagno- sis, prior intermittent ADT, previous radiation therapy, and/or systemic chemotherapy before RP, treatment with antiandrogens (AA) monotherapy, or insufficient clinical data precluding reliable on- cological assessment were excluded. A total of 84 patients met the inclusion criteria and were in- cluded in the final study cohort. The decision to administer NADT before surgery was made on an individual basis, primarily in patients with high- or very high-risk disease features. All patients subsequently underwent RP with standard pathological evaluation. Postoperative fol- low-up included serial PSA measurements per- formed according to the institutional protocols. ISSN 1812-9269. Experimental Oncology 48 (2). 2026 163 Dynamic Androgen Response Index and its Association with Biochemical Recurrence after Radical Prostatectomy The study protocol was approved by the ethics committee of the IU NAMS. The study was con- ducted in accordance with the principles of the Declaration of Helsinki. The median follow-up du- ration was 56 months (IQR 38—82). Neoadjuvant androgen-deprivation therapy. NADT consisted of luteinizing hormone-releasing hormone (LHRH) agonists administered as monotherapy or in combination with nonsteroidal AA. A combined androgen blockade was prefer- entially used in patients with high-risk disease characteristics. The duration of NADT ranged from 1 to 12 months and was determined by clin- ical considerations, tumor burden, and treatment planning factors. Calculations of biomarker dynamics. To quan- tify the rate of biomarker change during NADT, logarithmic slopes of PSA and testosterone decline were calculated. For each patient, the slope of PSA decline (kPSA) was estimated using the following equation: � � � �� � ln lnbaseline NADTPSA PSA kPSA t where PSAbaseline represents the PSA level before ini- tiation of NADT, PSANADT is the PSA level mea- sured after therapy, and t is the duration of NADT in months. Similarly, the slope of testosterone decline (kT) was calculated as: � � � �ln lnbaseline NADTT T kT t � � where Tbaseline and TNADT correspond to the serum testosterone levels before and after NADT. Based on these parameters, a dynamic IAR was defined as the ratio between the slopes of PSA and testosterone decline:  kPSAIAR kT � This index reflects the relationship between tu- mor PSA response and the degree of androgen sup- pression. Study endpoint. The primary endpoint of the study was BCR after RP. BCR was defined as a post- operative PSA level ≥ 0.2 ng/mL confirmed by a second measurement. Biochemical recurrence-free survival (BCRFS) was calculated from the date of RP to the date of BCR or the last follow-up. Statistical analysis. Descriptive statistics were used to summarize baseline clinical and patho- logical characteristics of the study cohort. The continuous variables were reported as medians with interquartile ranges (IQRs), whereas the cat- egorical variables were reported as frequencies and percentages. Comparisons between patients with and without biochemical recurrence (BCR) were performed us- ing the Mann—Whitney U-test for continuous variables and the Chi-square test or Fisher’s exact test for categorical variables, as appropriate. The BCRFS was estimated using the Kaplan— Meier method, and differences between groups were assessed using the log-rank test. To identify factors associated with BCR, an ini- tial univariable Cox proportional hazards regres- sion analysis was performed. Variables demonstrat- ing potential prognostic significance in the univari- able Cox regression were included in an initial multivariable Cox regression model. Variables that retained statistical significance in the initial multi- variable analysis were subsequently included in the final reduced prognostic model used for nomo- gram construction. Therefore, the initial multivari- able Cox regression model and the final reduced predictive model were presented separately. Based on the independent predictors identified in the multivariable analysis, a predictive model for BCR was constructed and presented as a nomo- gram. The discriminatory performance of the model was evaluated using the receiver operating characteristic (ROC) curve analysis, with the area under the curve (AUC) and corresponding 95% confidence intervals reported. The model calibration was assessed using cali- bration plots comparing the predicted probabilities with the observed outcomes. An internal validation of the model was performed using bootstrap resa- mpling (1,000 iterations) to estimate model opti- mism and stability. The potential clinical utility of the predictive model was further evaluated using a decision curve analysis, which quantified the net benefit of the model across a range of threshold probabilities. All statistical analyses were performed using IBM SPSS Statistics version 22 (IBM Corp., Ar- monk, NY, USA), GraphPad Prism version 10.4.1 164 ISSN 1812-9269. Experimental Oncology 48 (2). 2026 Ye. Afanasiev, S. Vozianov, R. Danylets, V. Grygorenko, O. Shulyak, M. Sosnin, I. Kholokharenko (GraphPad Software, Boston, MA, USA), and R statistical software version 4.5.2. A two-sided p-va lue < 0.05 was considered statistically significant. Results Patient characteristics. A total of 84 patients were included in the study. Detailed baseline character- istics are summarized in Table 1. The baseline characteristics in patients with and without BCR are compared in Table 2. Patients who developed BCR had significantly higher baseline PSA levels compared to patients without recur- rence (26.1 vs 12.2 ng/mL, p = 0.007). Baseline tes- tosterone levels were significantly lower in patients who experienced BCR (11.1 vs 15.1 nmol/L, p < 0.001), while testosterone levels after neoadju- vant therapy were significantly higher (1.4 vs 0.6 nmol/L, p < 0.001). Clinical stage ≥ cT3a was significantly more frequent among patients with re- currence (p = 0.002). Similarly, patients classified as high-risk according to NCCN criteria demon- strated a higher likelihood of BCR (p = 0.01). The other clinical parameters, including body mass in- dex, prostate volume, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and Charlson comorbidity index, were not significantly associ- ated with recurrence. Cox regression analysis. Univariable Cox regres- sion analysis identified several predictors associ- ated with BCR, including clinical stage ≥ cT3a (HR 2.52, 95% CI: 1.49—4.28, p = 0.001), testosterone level after neoadjuvant therapy (HR 2.48, 95% CI: 1.42—4.35, p = 0.001), log-transformed PSA (HR 1.52, 95% CI: 1.13—2.06, p = 0.006), and the IAR (HR 1.60, 95% CI: 1.18—2.17, p = 0.003). In the multivariable model, clinical stage ≥ cT3a (HR 2.34, 95% CI: 1.34—4.07, p = 0.003) and IAR (HR 1.48, 95% CI: 1.05—2.09, p = 0.024) remained independent predictors of BCR. Although log-trans- formed PSA was significant in the univariable anal- ysis, its significance was lost in the multivariable model. The results of the Cox regression analysis are summarized in Table 3. In the initial multivariable model including the clinical stage ≥ cT3a, IAR, and log-transformed PSA, clinical stage ≥ cT3a and IAR remained independently associated with BCR, whereas log-transformed PSA was not statistically significant. Therefore, log-transformed PSA was not retained in the final reduced predictive model. Table 1. Baseline clinical and laboratory characteristics of the study population Parameter Overall (n = 84) Clinical parameters Age, years, median (IQR) 64.5 (60.0—68.7) BMI, kg/m2, median (IQR) 27.8 (25.3—30.0) Baseline PSA (PSA0), ng/mL, median (IQR) 20.9 (11.5—38.3) Post-NADT PSA (PSA1), ng/mL, median (IQR) 1.1 (0.4—3.9) Baseline testosterone (T0), nmol/L, median (IQR) 14.3 (11.5—16.7) Post-NADT testosterone (T1), nmol/L, median (IQR) 0.7 (0.5—1.1) Initial prostate volume, cm³, median (IQR) 55.0 (42.7—75.8) NADT prostate volume, cm³, median (IQR) 36.1 (24.7—53.1) NLR, median (IQR) 3.0 (2.3—4.2) PLR, median (IQR) 156.8 (121.7—216.3) CCI, median (IQR) 2.0 (2—3) Biopsy ISUP grade ISUP <4 ISUP ≥4 64 (76.2%) 20 (23.8%) Clinical stage ≤ T2c ≥ T3a 54 (64.3%) 30 (35.7%) NCCN Risk Group ≤ Intermediate risk ≥ High risk 27 (32.1%) 57 (67.9%) Duration of NADT 1—2 months 3 months >3 months 28 (33.3%) 29 (34.5%) 27 (32.1%) Type of NADT LHRH agonists LHRH agonists + AA 54 (64.3%) 30 (35.7%) Notes: IQR — interquartile range; NADT — neoadjuvant androgen deprivation therapy; BMI — body mass index; PSA0  — initial PSA level; PSA1  — post-NADT PSA level; T0  — initial testosterone level; T1  — post-NADT testosterone level; NLR — neutrophil-to-lymphocyte ratio; PLR  — platelet-to-lymphocyte ratio; CCI  — Charlson’s comorbidity index; ISUP  — International Society of Urological Pathology; NCCN — National Comprehensive Cancer Network; LHRH — luteinizing hormone-releasing hormone; AA — antiandrogens. ISSN 1812-9269. Experimental Oncology 48 (2). 2026 165 Dynamic Androgen Response Index and its Association with Biochemical Recurrence after Radical Prostatectomy Development of the predictive model. Based on the independent predictors identified in the mul- tivariable analysis, a predictive model incorporat- ing clinical stage ≥ cT3a and the IAR was devel- oped (Table 4). A nomogram was plotted to esti- mate the probability of BCR within 36 months following RP (Fig. 1). The discriminative ability of the model was eva luated using Harrell’s concordance index. The model demonstrated moderate discrimination with a C-index of 0.64. Time-dependent ROC analysis at 36 months yielded an AUC of 0.68 (95% CI 0.56—0.80) (Fig. 2). The Brier score was 0.221 (95% CI: 0.181—0.260), indicating acceptable pre- dictive accuracy. An internal validation using boot- strap resampling (1000 iterations) yielded a Somer’s Dxy of 0.27, corresponding to a C-index of 0.64, indicating stable predictive performance. Table 2. Comparison of baseline characteristics between patients with and without BCR Parameter BCR (n = 62) No BCR (n = 22) p Clinical parameters Age, years, median (IQR) 64.0 (60.0—68.0) 66.5 (60.7—69.2) 0.384* BMI, kg/m2, median (IQR) 27.6 (24.8—29.9) 28.1 (26.0—30.6) 0.368* Baseline PSA (PSA0), ng/mL, median (IQR) 26.1 (13.9—46.5) 12.2 (8.8—27.1) 0.007* Post-NADT PSA (PSA1), ng/mL, median (IQR) 1.25 (0.4—5.0) 0.65 (0.2—3.1) 0.136* Baseline testosterone (T0), nmol/L, median (IQR) 11.1 (9.3—13.9) 15.1 (13.5—16.9) <0.001* Post-NADT testosterone (T1), nmol/L, median (IQR) 1.4 (0.7—1.6) 0.6 (0.4—0.9) <0.001* Initial prostate volume, cm³, median (IQR) 54.5 (42.5—73.6) 56.7 (43.6—81.9) 0.522* NADT prostate volume, cm³, median (IQR) 36.1 (23.4—47.7) 35.4 (29.2—70.1) 0.242* NLR, median (IQR) 2.9 (2.3—4.0) 3.0 (2.0—4.5) 0.737* PLR, median (IQR) 155.9 (123.4—220.7) 165.1 (109.9—201.2) 0.919* CCI, median, (IQR) 2 (2—3) 2 (2—3) 0.776χ² Biopsy ISUP grade ISUP <4 ISUP ≥4 45 (72.6%) 17 (27.4%) 19 (86.4%) 3 (13.6%) 0.156χ² Clinical stage ≤ T2c ≥ T3a 34 (54.8%) 28 (45.2%) 20 (90.9%) 2 (9.1%) 0.002χ² NCCN Risk Group ≤ Intermediate-risk ≥ High-risk 15 (24.2%) 47 (75.8%) 12 (54.5%) 10 (45.5%) 0.01χ² Duration of NADT Duration 1—2 months Duration 3 months Duration > 3 months 21 (33.9%) 19 (30.6%) 22 (35.5%) 7 (31.8%) 10 (45.5%) 5 (22.7%) 0.392χ² Type of NADT LHRH agonists LHRH agonists + AA 39 (62.9%) 23 (37.1%) 15 (68.2%) 7 (31.8%) 0.431χ² Notes: * Comparison of groups by Mann-Whitney test; χ² Comparison of categorical variables by chi-square test; BCR — biochemical recurrence; IQR — interquartile range; NADT — neoadjuvant androgen deprivation therapy; BMI — body mass index; PSA0 — initial PSA level; PSA1 — post-NADT PSA level; T0 — initial testosterone level; T1 — post-NADT testosterone level; NLR  — neutrophil-to-lymphocyte ratio; PLR  — platelet-to-lymphocyte ratio; CCI  — Charlson’s comorbidity index; ISUP — International Society of Urological Pathology; NCCN — National Comprehensive Cancer Network; LHRH — luteinizing hormone-releasing hormone; AA — antiandrogens. 166 ISSN 1812-9269. Experimental Oncology 48 (2). 2026 Ye. Afanasiev, S. Vozianov, R. Danylets, V. Grygorenko, O. Shulyak, M. Sosnin, I. Kholokharenko Calibration analysis demonstrated good agreement between predicted and observed probabilities of BCR at 36 months (Fig. 3). The calibration curve indicated satisfactory agreement between predicted and ob- served event rate across the range of predicted risks. Decision curve analysis demonstrated that the predictive model provided a higher net benefit compared to both the “treat-all” and “treat-none” strategies across a wide range of threshold proba- bilities (Fig. 4). These findings suggest that the model may provide clinically useful risk stratifica- tion for patients undergoing RP after NADT. Risk stratification analysis. To evaluate the cli nical applicability of the proposed predictive mo del, the patients were stratified into three risk groups (low, intermediate, and high) according to the calculated risk score derived from the multi- variable Cox regression model. Kaplan—Meier analysis demonstrated a clear sep- aration of BCRFS among the three groups (Fig. 5). The median BCRFS was 69 months in the low-risk group, 57 months in the intermediate-risk group, and 26 months in the high-risk group. Similarly, the estimated mean BCRFS was 62.4 months in the low-risk group, 53.9 months in the intermediate- risk group, and 29.6 months in the high-risk group, indicating a progressive reduction in survival with increasing risk. The differences in survival distri- butions between the groups were significant ac- cording to the log-rank test (χ2 = 18.95, p < 0.001). These findings support the proposed model’s abili ty to effectively stratify patients by risk of BCR fol- lowing RP. Discussion The present study evaluated the prognostic sig- nificance of a novel IAR and developed a predic- tive model for BCR in patients undergoing NADT before RP. The main findings of this study are threefold. First, the IAR integrating PSA and tes- tosterone kinetics during NADT was indepen- dently associated with BCR. Second, the integra- tion of IAR with the clinical stage enabled the de- velopment of a simple predictive model capable of stratifying patients into distinct recurrence-risk groups. Third, the proposed model demonstrated acceptable discrimination, satisfactory calibra- tion, and potential clinical benefit in decision curve analysis. Neoadjuvant systemic therapy before RP has been investigated for several decades. Early ran- domized trials demonstrated that NADT could reduce prostate volume, tumor burden, and the rate of positive surgical margins. However, these studies failed to consistently demonstrate im- provements in long-term oncologic outcomes such as BCR or OS [6, 7]. For instance, Soloway et al. [6] reported improved pathological findings Table 3. Cox proportional hazard regression analysis for predictors of biochemical recurrence Parameter HR 95% CI p Univariable analysis cT ≥ T3a 2.52 1.49—4.28 0.001 ISUP Grade after biopsy ≥ ISUP 4 1.29 0.74—2.25 0.378 Baseline testosterone (T0) 0.84 0.77—0.91 < 0.001 Post-NADT testosterone (T1) 2.48 1.42—4.35 0.001 kPSA 0.94 0.69—1.29 0.716 kT 0.69 0.49—0.96 0.027 Log (PSA) 1.52 1.13—2.06 0.006 IAR 1.60 1.18—2.17 0.003 Multivariable analysis cT ≥ T3a 2.34 1.34—4.07 0.003 IAR 1.48 1.05—2.09 0.024 Log (PSA) 1.17 0.84—1.62 0.367 Notes: HR — hazard ratio; CI — confidence interval; cT — clinical stage; ISUP — International Society of Urological Pathology; T0  — initial testosterone level; T1  — post- NADT testosterone level; kPSA  — logarithmic slope of PSA level decline; kT — logarithmic slope of testosterone level decline; IAR — dynamic androgen response index. Fig. 1. Nomogram for prediction of biochemical recur- rence at 36 months: IAR  — androgen response index; BCR — biochemical recurrence ISSN 1812-9269. Experimental Oncology 48 (2). 2026 167 Dynamic Androgen Response Index and its Association with Biochemical Recurrence after Radical Prostatectomy the degree of achieved androgen suppression may provide an incomplete representation of biological treatment response. The present study addresses this limitation by integrating PSA and testosterone kinetics into a composite biomarker — the IAR. Since the prima- ry mechanism of ADT action involves suppression of circulating testosterone levels, evaluating PSA decline relative to testosterone suppression may following neoadjuvant androgen ablation but no significant reduction in BCR after surgery. Simi- larly, Schulman et al. [7] observed favorable path- ological outcomes without a clear survival advan- tage in patients receiving neoadjuvant hormonal therapy before RP. In recent years, interest in neoadjuvant therapy has re-emerged due to the development of intensi- fied androgen receptor signaling inhibitors (ARSI) and combination systemic approaches. Current studies suggest that neoadjuvant treatment with AR-targeted agents such as abiraterone, enzalu- tamide, or apalutamide can induce significant pathological responses in patients with high-risk localized PCa, including the rates of minimal re- sidual disease and complete pathological response [13—16]. Despite these promising results, patient selection remains a major challenge, as tumors ex- hibit heterogeneous biological sensitivity to andro- gen deprivation. Several recent reviews emphasize that reliable predictive biomarkers capable of selecting patients most likely to benefit from neoadjuvant therapy represent a major unmet clinical need in localized high-risk and locally advanced PCa [17]. In this context, dynamic biomarkers reflecting biological response during treatment may provide clinically valuable information about tumor endocrine sen- sitivity and treatment responsiveness. PSA kinetics have long been investigated as a marker of treatment response in PCa. Several stu dies have demonstrated that dynamic PSA chan ges during hormonal therapy may provide prog- nostic information beyond baseline PSA measure- ments. Foo et al. [10] reported that PSA kinetics during neoadjuvant hormonal treatment was as- sociated with BCR and PCa-mortality. Similarly, Kang et al. [11] demonstrated that PSA half-life during neoadjuvant hormone therapy predicted the risk of developing castration-resistant PCa fol- lowing RP. McDonald et al. [12] further con- firmed that PSA response to NADT may serve as an independent prognostic indicator of oncologic outcomes. Despite these findings, PSA dynamics alone may not fully reflect tumor sensitivity to an- drogen deprivation. PSA reduction during ADT may reflect both suppression of androgen-de- pendent PSA production and intrinsic tumor susceptibility to endocrine therapy. Therefore, PSA kinetics evaluated without consideration of Fig. 3. Calibration plot demonstrating agreement between predicted and observed probabilities of biochemical re- currence at 36 months. AUC and Brier score values shown in the figure are expressed as percentages Se ns iti vi ty , % Fig. 2. ROC curve for prediction of biochemical recur- rence at 36 months based on the final Cox regression model: AUC — area under the curve 168 ISSN 1812-9269. Experimental Oncology 48 (2). 2026 Ye. Afanasiev, S. Vozianov, R. Danylets, V. Grygorenko, O. Shulyak, M. Sosnin, I. Kholokharenko better reflect the biological responsiveness of PCa to endocrine treatment. However, this interpreta- tion should be considered hypothesis-generating. The present study did not directly evaluate mole cular, genetic, or pathological markers of androgen sensitivity. Therefore, IAR should be interpreted as a potential surrogate dynamic marker of response to androgen deprivation rather than as a definitive marker of biological sensitivity. In our analysis, IAR remained independently associated with BCR in multivariable Cox regression analysis, while PSA- related parameters lost statistical significance after adjustment. These findings suggest that composite biomarker dynamics may capture clinically rele- vant biological information beyond conventional PSA measures alone. Based on the results, a predictive model incor- porating clinical stage and IAR was developed and presented as a nomogram. The model demonstra ted moderate discrimination with a concordance index of approximately 0.64 and a time-dependent AUC of approximately 0.67 for prediction of BCR in 36 months. Although these values indicate mo derate predictive accuracy, similar levels of dis- crimination have been reported in several widely used clinical prediction models for PCa. Classic predictive tools such as the Partin tables, CAPRA score, and Kattan nomograms rely primarily on baseline clinicopathological parameters, including PSA level, biopsy Gleason grade, and clinical stage [18—20]. While these models remain useful in clinical practice, they primarily reflect tumor bur- den and disease extent rather than biological re- sponse to therapy. In contrast, the model pro- posed in the present study incorporates dynamic treatment-response biomarkers obtained during neoadjuvant therapy. By combining tumor bio- marker kinetics with endocrine suppression pa- rameters, the IAR may represent a more biologi- cally informative measure of treatment sensitivity. Another important feature of the present model is its simplicity. The final model includes only two variables — clinical stage ≥cT3a and the IAR, which facilitates straightforward clinical imple- mentation in routine practice. Compared with more complex models, requiring multiple clinico- pathological variables of postoperative pathologi- cal data, such parsimony may enhance reprodu cibility and applicability in routine clinical prac- tice. The integration of biomarker kinetics and endocrine suppression parameters may therefore represent a promising strategy for identifying pa- tients with biologically sensitive tumors, who are more likely to benefit from NADT. Importantly, the proposed model demonstrated the ability to stratify patients into low-, interme diate-, and high-risk groups with significantly dif- ferent BCRFS. This risk stratification may have po- Fig. 4. Decision curve analysis evaluating the clinical util- ity of the predictive model Fig. 5. Kaplan–Meier curves for biochemical recurrence- free survival according to the risk groups derived from the predictive model. BCR — biochemical recurrence; BCRFS — biochemical recurrence-free survival ISSN 1812-9269. Experimental Oncology 48 (2). 2026 169 Dynamic Androgen Response Index and its Association with Biochemical Recurrence after Radical Prostatectomy tential clinical implications, particularly in the con- text of neoadjuvant therapy. Patients demonstrating poor biological response to NADT may represent a subgroup at increased risk of early recurrence de- spite surgery and may therefore benefit from closer postoperative monitoring or consideration of mul- timodal treatment strategies. From a clinical per- spective, early identification of patients demon- strating poor biological response to NADT may be particularly relevant. Such patients could potential- ly benefit from intensified postoperative surveil- lance, earlier initiation of adjuvant therapies, or en- rollment in clinical trials evaluating multimodal treatment strategies. The findings of this study should be interpret- ed in light of several limitations. First, the retro- spective and single-center design may introduce potential selection bias. Second, the relatively modest sample size may limit statistical power and the generalizability of the results. Third, al- though internal validation was performed, exter- nal validation in independent cohorts is required before the proposed model can be implemented in routine clinical practice. In addition, the in- cremental prognostic value of IAR compared with models based solely on conventional static biomarkers or individual components of the in- dex was not directly assessed. Future studies should evaluate whether the addition of IAR im- proves the model discrimination, calibration, and clinical utility beyond established clinical and laboratory predictors. Another limitation relates to the treatment regimens used in this cohort. The study population primarily received conven- tional ADT with LHRH agonists and AA rather than modern intensified androgen receptor sig- naling inhibitors. Therefore, the predictive per- formance of the proposed model in patients treated with contemporary intensified neoadju- vant regimens remains to be determined. Future studies, including larger multicenter cohorts and patients treated with modern intensified ARSI, will be necessary to validate and further refine the proposed model. Despite these limitations, the study has several strengths. It proposes a biologically plausible com- posite biomarker reflecting endocrine treatment re- sponse, applies a comprehensive predictive mode ling workflow including Cox regression, nomogram development, calibration analysis, and decision curve analysis, and demonstrates clinically meaning- ful survival-based risk stratification. In a field where the main challenge is identifying patients who may truly benefit from neoadjuvant therapy, biomarker- oriented approaches such as the one proposed in this study may contribute to more individualized treat- ment strategies. Importantly, the present findings should be interpreted in an exploratory context. The proposed index may reflect biological tumor respon- siveness to androgen deprivation; however, it should not be considered a definitive predictive tool without external validation. In sum, the present study introduces a novel dy- namic androgen response index integrating PSA and testosterone kinetics during NADT. The pro- posed index was independently associated with BCR and enabled the development of a simple pre- dictive model capable of stratifying patients ac- cording to their risk of recurrence following RP. IAR may serve as a surrogate dynamic marker of response to androgen deprivation. However, this interpretation remains hypothesis-generating and requires further validation. Further prospective studies with external validation are warranted to confirm the clinical applicability of this approach and to explore its potential role in guiding perso nalized neoadjuvant treatment strategies in PCa. Conflict of interests The authors declare no conflict of interest. Funding This research received no external funding. Ethics approval and informed consent The study was conducted in accordance with the Declaration of Helsinki and approved by the Ethics Committee of the IU NAMS (Protocol #6, 14 De- cember 2023). Given the retrospective design of the study, the requirement for informed consent was waived by the ethics committee. 170 ISSN 1812-9269. Experimental Oncology 48 (2). 2026 Ye. Afanasiev, S. Vozianov, R. Danylets, V. Grygorenko, O. Shulyak, M. Sosnin, I. Kholokharenko REFERENCES 1. Sung H, Ferlay J, Siegel RL, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mor- tality worldwide for 36 cancers in 185 countries. 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Experimental Oncology 48 (2). 2026 171 Dynamic Androgen Response Index and its Association with Biochemical Recurrence after Radical Prostatectomy Є. Афанас’єв 1, 2, С. Возіанов 1, 2, Р. Данилець 1, 2, В. Григоренко 1, О. Шуляк 2, М. Соснін 1, 2, І. Холохаренко 2 1 ДУ «Інститут урології ім. акад. О.Ф.Возіанова НАМН України», Київ, Україна 2 Кафедра урології, Національного університету охорони здоров’я України імені П.Л. Шупика, Київ, Україна ДИНАМІЧНИЙ ІНДЕКС АНДРОГЕННОЇ ВІДПОВІДІ ТА ЙОГО АСОЦІАЦІЯ З БІОХІМІЧНИМ РЕЦИДИВОМ ПІСЛЯ РАДИКАЛЬНОЇ ПРОСТАТЕКТОМІЇ НА ТЛІ НЕОАД‘ЮВАНТНОЇ АНДРОГЕН-ДЕПРИВАЦІЙНОЇ ТЕРАПІЇ Стан питання. Неоад’ювантна андроген-деприваційна терапія (НАДТ) перед радикальною простатектомі- єю (РПЕ) залишається дискусійним підходом у лікуванні локалізованого та місцево розповсюдженого раку передміхурової залози (РПЗ). Одним з основних обмежень цієї стратегії є відсутність біомаркерів, які могли б ідентифікувати пухлини, біологічно чутливі до андрогенної депривації. Метою цього дослідження було оцінити прогностичне значення динамічного індексу андрогенної відповіді (ІАВ) та розробити прогностич- ну модель біохімічного рецидиву (БР) після РПЕ. Матеріали та методи. У ретроспективне одноцентрове дослідження було включено 84 пацієнти з РПЗ, які отримували НАДТ із подальшим виконанням РПЕ. ІАВ визначали як співвідношення логарифмічних нахилів зниження концентрацій простат-специфічного анти- гену (ПСА) та тестостерону під час неоад’ювантної терапії. Для визначення предикторів БР проведено ре- гресійний аналіз Кокса. На основі незалежних предикторів побудовано прогностичну модель та номограму. Ефективність цієї моделі оцінювали за допомогою ROC-аналізу, калібрувальних графіків та аналізу кривих клінічної доцільності. Результати. Протягом спостереження (медіана 56 місяців) БР виник у 62 пацієнтів. Клінічна стадія ≥ cT3a (ВР 2,34; p = 0,003) та ІАВ (ВР 1,48; p = 0,024) були незалежними предикторами БР. Мо- дель продемонструвала помірну дискримінаційну здатність (AUC 0,68) та задовільне калібрування. Висно- вки. ІАВ може відображати біологічну відповідь РПЗ на андрогенну депривацію. Отримані результати слід розглядати як такі, що генерують гіпотезу, і вони потребують подальшої валідації в контексті стратифікації ризику БР після РПЕ на тлі НАДТ. Ключові слова: рак передміхурової залози, неоад’ювантна андроген-деприваційна терапія, біохімічний реци- див, прогностична модель, номограма, андрогенна відповідь.
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spelling oai:ojs2.ex.aqua-time.com.ua:article-6512026-08-21T12:36:14Z DYNAMIC ANDROGEN RESPONSE INDEX AND ITS ASSOCIATION WITH BIOCHEMICAL RECURRENCE AFTER RADICAL PROSTATECTOMY FOLLOWING NEOADJUVANT ANDROGEN DEPRIVATION THERAPY ДИНАМІЧНИЙ ІНДЕКС АНДРОГЕННОЇ ВІДПОВІДІ ТА ЙОГО АСОЦІАЦІЯ З БІОХІМІЧНИМ РЕЦИДИВОМ ПІСЛЯ РАДИКАЛЬНОЇ ПРОСТАТЕКТОМІЇ НА ТЛІ НЕОАД‘ЮВАНТНОЇ АНДРОГЕН-ДЕПРИВАЦІЙНОЇ ТЕРАПІЇ Afanasiev, Ye. Vozianov, S. Danylets, R. Grygorenko, V. Shulyak, O. Sosnin, M. Kholokharenko, I. рак передміхурової залози, неоад’ювантна андроген-деприваційна терапія, біохімічний рецидив, прогностична модель, номограма, андрогенна відповідь prostate cancer, neoadjuvant androgen deprivation therapy, biochemical recurrence, predictive model, nomogram, androgen response Background. Neoadjuvant androgen deprivation therapy (NADT) before radical prostatectomy (RP) remains controversial in the management of localized and locally advanced prostate cancer. A major limitation of this strategy is the lack of biomarkers capable of identifying tumors biologically sensitive to androgen deprivation. This study aimed to evaluate the prognostic value of a dynamic androgen response index (IAR) and develop a predictive model for biochemical recurrence (BCR) after RP. Materials and Methods. The retrospective single-center study included 84 patients with prostate cancer treated with NADT followed by RP. The IAR was defined as the ratio between logarithmic slopes of PSA and testosterone decline during neoadjuvant therapy. Cox regression analysis was performed to identify predictors of BCR. A predictive model and nomogram were constructed based on independent predictors. Model performance was evaluated using ROC analysis, calibration plots, and decision curve analysis. Results. During a median follow-up of 56 months, BCR occurred in 62 patients. Clinical stage ≥ cT3a (HR 2.34; p = 0.003) and IAR (HR 1.48; p = 0.024) were independent predictors of BCR. The model demonstrated moderate discrimination (AUC 0.68) and satisfactory calibration. Conclusions. The IAR may reflect the biological responsiveness of prostate cancer to androgen deprivation. These findings should be considered hypothesis-generating and require further validation in the context of risk stratification for BCR following RP after NADT. Стан питання. Неоад’ювантна андроген-деприваційна терапія (НАДТ) перед радикальною простатектомією (РПЕ) залишається дискусійним підходом у лікуванні локалізованого та місцево розповсюдженого раку передміхурової залози (РПЗ). Одним з основних обмежень цієї стратегії є відсутність біомаркерів, які могли б ідентифікувати пухлини, біологічно чутливі до андрогенної депривації. Метою цього дослідження було оцінити прогностичне значення динамічного індексу андрогенної відповіді (ІАВ) та розробити прогностичну модель біохімічного рецидиву (БР) після РПЕ. Матеріали та методи. У ретроспективне одноцентрове дослідження було включено 84 пацієнти з РПЗ, які отримували НАДТ із подальшим виконанням РПЕ. ІАВ визначали як співвідношення логарифмічних нахилів зниження концентрацій простат-специфічного антигену (ПСА) та тестостерону під час неоад’ювантної терапії. Для визначення предикторів БР проведено регресійний аналіз Кокса. На основі незалежних предикторів побудовано прогностичну модель та номограму. Ефективність цієї моделі оцінювали за допомогою ROC-аналізу, калібрувальних графіків та аналізу кривих клінічної доцільності. Результати. Протягом спостереження (медіана 56 місяців) БР виник у 62 пацієнтів. Клінічна стадія ≥ cT3a (ВР 2,34; p = 0,003) та ІАВ (ВР 1,48; p = 0,024) були незалежними предикторами БР. Модель продемонструвала помірну дискримінаційну здатність (AUC 0,68) та задовільне калібрування. Висновки. ІАВ може відображати біологічну відповідь РПЗ на андрогенну депривацію. Отримані результати слід розглядати як такі, що генерують гіпотезу, і вони потребують подальшої валідації в контексті стратифікації ризику БР після РПЕ на тлі НАДТ. PH Akademperiodyka 2026-08-21 Article Article application/pdf https://exp-oncology.com.ua/index.php/Exp/article/view/651 10.15407/exp-oncology.2026.02.161 Experimental Oncology; Vol. 48 No. 2 (2026): Experimental Oncology; 161-171 Експериментальна онкологія; Том 48 № 2 (2026): Експериментальна онкологія; 161-171 2312-8852 1812-9269 10.15407/exp-oncology.2026.02 en https://exp-oncology.com.ua/index.php/Exp/article/view/651/477 Copyright (c) 2026 Experimental Oncology https://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle рак передміхурової залози
неоад’ювантна андроген-деприваційна терапія
біохімічний рецидив
прогностична модель
номограма
андрогенна відповідь
Afanasiev, Ye.
Vozianov, S.
Danylets, R.
Grygorenko, V.
Shulyak, O.
Sosnin, M.
Kholokharenko, I.
ДИНАМІЧНИЙ ІНДЕКС АНДРОГЕННОЇ ВІДПОВІДІ ТА ЙОГО АСОЦІАЦІЯ З БІОХІМІЧНИМ РЕЦИДИВОМ ПІСЛЯ РАДИКАЛЬНОЇ ПРОСТАТЕКТОМІЇ НА ТЛІ НЕОАД‘ЮВАНТНОЇ АНДРОГЕН-ДЕПРИВАЦІЙНОЇ ТЕРАПІЇ
title ДИНАМІЧНИЙ ІНДЕКС АНДРОГЕННОЇ ВІДПОВІДІ ТА ЙОГО АСОЦІАЦІЯ З БІОХІМІЧНИМ РЕЦИДИВОМ ПІСЛЯ РАДИКАЛЬНОЇ ПРОСТАТЕКТОМІЇ НА ТЛІ НЕОАД‘ЮВАНТНОЇ АНДРОГЕН-ДЕПРИВАЦІЙНОЇ ТЕРАПІЇ
title_alt DYNAMIC ANDROGEN RESPONSE INDEX AND ITS ASSOCIATION WITH BIOCHEMICAL RECURRENCE AFTER RADICAL PROSTATECTOMY FOLLOWING NEOADJUVANT ANDROGEN DEPRIVATION THERAPY
title_full ДИНАМІЧНИЙ ІНДЕКС АНДРОГЕННОЇ ВІДПОВІДІ ТА ЙОГО АСОЦІАЦІЯ З БІОХІМІЧНИМ РЕЦИДИВОМ ПІСЛЯ РАДИКАЛЬНОЇ ПРОСТАТЕКТОМІЇ НА ТЛІ НЕОАД‘ЮВАНТНОЇ АНДРОГЕН-ДЕПРИВАЦІЙНОЇ ТЕРАПІЇ
title_fullStr ДИНАМІЧНИЙ ІНДЕКС АНДРОГЕННОЇ ВІДПОВІДІ ТА ЙОГО АСОЦІАЦІЯ З БІОХІМІЧНИМ РЕЦИДИВОМ ПІСЛЯ РАДИКАЛЬНОЇ ПРОСТАТЕКТОМІЇ НА ТЛІ НЕОАД‘ЮВАНТНОЇ АНДРОГЕН-ДЕПРИВАЦІЙНОЇ ТЕРАПІЇ
title_full_unstemmed ДИНАМІЧНИЙ ІНДЕКС АНДРОГЕННОЇ ВІДПОВІДІ ТА ЙОГО АСОЦІАЦІЯ З БІОХІМІЧНИМ РЕЦИДИВОМ ПІСЛЯ РАДИКАЛЬНОЇ ПРОСТАТЕКТОМІЇ НА ТЛІ НЕОАД‘ЮВАНТНОЇ АНДРОГЕН-ДЕПРИВАЦІЙНОЇ ТЕРАПІЇ
title_short ДИНАМІЧНИЙ ІНДЕКС АНДРОГЕННОЇ ВІДПОВІДІ ТА ЙОГО АСОЦІАЦІЯ З БІОХІМІЧНИМ РЕЦИДИВОМ ПІСЛЯ РАДИКАЛЬНОЇ ПРОСТАТЕКТОМІЇ НА ТЛІ НЕОАД‘ЮВАНТНОЇ АНДРОГЕН-ДЕПРИВАЦІЙНОЇ ТЕРАПІЇ
title_sort динамічний індекс андрогенної відповіді та його асоціація з біохімічним рецидивом після радикальної простатектомії на тлі неоад‘ювантної андроген-деприваційної терапії
topic рак передміхурової залози
неоад’ювантна андроген-деприваційна терапія
біохімічний рецидив
прогностична модель
номограма
андрогенна відповідь
topic_facet рак передміхурової залози
неоад’ювантна андроген-деприваційна терапія
біохімічний рецидив
прогностична модель
номограма
андрогенна відповідь
prostate cancer
neoadjuvant androgen deprivation therapy
biochemical recurrence
predictive model
nomogram
androgen response
url https://exp-oncology.com.ua/index.php/Exp/article/view/651
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