ЗНАЧЕННЯ ЕКСПРЕСІЇ ПУХЛИНОАСОЦІЙОВАНИХ МІКРОРНК-21 ТА -375 ДЛЯ ПРОГНОЗУВАННЯ ПЕРЕБІГУ РАКУ ПОРОЖНИНИ РОТА

• Дата: 2023
• Автори: Kravets, О.V., Burtyn, O.V., Borikun, T.V., Zadvornyi, T.V., Yalovenko, T.M., Kolesnik, O.O.
• Формат: Стаття
• Мова: English
• Опубліковано: PH Akademperiodyka 2023
• Теми: рак ротової порожнини; мікроРНК-21; мікроРНК-375; виживаність; агресивність.
• Онлайн доступ: https://exp-oncology.com.ua/index.php/Exp/article/view/2021-1-10
• Назва журналу: Experimental Oncology

Aim: To investigate the features of expression of miRNA-21 and miRNA-375 in tumor cells of patients with cancer of oral cavity (COC) and to determine the possibility of their use to predict the aggressiveness of COC course. Materials and Methods: The work is based on the results of examination and treatment of 50 patients with stage II–IV COC. miRNA expression in tumor cells was analyzed by real time reverse transcription polymerase chain reaction. Results: High levels of miRNA-21 expression (> 0.26 a.u.) and miRNA-375 (> 0.36 a.u.) were determined in 72.0% and 63.0% of cases. We revealed a tendency to decreased miRNA-21 expression and increased miRNA-375 expression in tumors of patients with recurrence-free survival less than 12 months and the presence of metastatic lesions in regional lymph nodes. In patients with COC of low differentiation grade, the level of miRNA-21 was 2.0 times lower compared with tumors of moderate differentiation grade (p < 0.05), while the expression of miRNA-375, on the contrary, was higher in tumors of low differentiation grade. A decreased expression of miRNA-21 (< 0.26 a.u.) against the background of decreased levels of miRNA-375 (< 0.36 a.u.) in tumor cells was associated with worse recurrence-free survival. Conclusions: The obtained results indicate the relation between the main clinical and pathological characteristics of patients with COC and the levels of miRNA-21 and -375 expression in tumor tissue, which indicates the involvement of these miRNAs in the formation of COC malignancy evidencing on their potential usefulness as additional prognostic markers.