ОЦЕНКА БИОРАЗНООБРАЗИЯ ЛЕСНЫХ НАСАЖДЕНИЙ ЛИСТВЕННЫХ ДРЕВЕСНЫХ ВИДОВ НА ОСНОВЕ МОЛЕКУЛЯРНОГО МАРКИРОВАНИЯ
Introduction Conservation and maintenance of biodiversity is promoted as an important criterion for sustainable forest management. In this regard, the improvement of methods for assessment and monitoring of biological diversity is an urgent task. The most convenient and informative tool for assessin...
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| Date: | 2018 |
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| Language: | Russian |
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Ukrainian Research Institute of Forestry and Forest Melioration named after G. M. Vysotsky (URIFFM)
2018
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| Online Access: | https://forestry-forestmelioration.org.ua/index.php/journal/article/view/112 |
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| Journal Title: | Forestry and Forest Melioration |
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Forestry and Forest Melioration| _version_ | 1856543327526060032 |
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| author | Padutov, V. E. Kagan, D. I. Baranov, O. Yu. Ivanovskaya, S. I. Razumova, O. A. Shestibratov, K. A. |
| author_facet | Padutov, V. E. Kagan, D. I. Baranov, O. Yu. Ivanovskaya, S. I. Razumova, O. A. Shestibratov, K. A. |
| author_sort | Padutov, V. E. |
| baseUrl_str | |
| collection | OJS |
| datestamp_date | 2019-04-27T15:16:57Z |
| description | Introduction
Conservation and maintenance of biodiversity is promoted as an important criterion for sustainable forest management. In this regard, the improvement of methods for assessment and monitoring of biological diversity is an urgent task.
The most convenient and informative tool for assessing the genetic diversity and structure of populations of living organisms is the methods of molecular genetic analysis. The use of molecular markers based on isoenzymes and polymorphic DNA fragments allows describing and differentiating genotypes of individuals with a high degree of reliability.
The aim of the study is to assess the level of genetic variability, subdivision and differentiation of forest plantations of deciduous tree species.
Materials and Methods
The objects of the study were plantations of deciduous tree species (Populus tremula L., Betula pendula Roth., Quercus robur L.), growing on the territory of Belarus, the bordering eastern regions (Moscow and Leningrad regions of the Russian Federation), the Republic of Tatarstan.
Molecular genetic analysis was done using isoenzyme and DNA-methods. The experimental material was diploid tissues of axillary buds and leaves. Homogenization, isolation and histochemical staining of the enzymes were carried out in accordance with generally accepted procedures with some modifications.
DNA analysis was performed using RAPD and SSR markers. DNA isolation, polymerase chain reaction, electrophoretic fractionation, and interpretation of data were made in accordance with generally accepted procedures.
Results and Conclusions
To assess the level of genetic variability for each of the species studied, the parameters of genetic diversity were calculated. It was shown that the analyzed tree species are characterized by wide range of indicators of genetic diversity. According to isozyme analysis at the species level, the share of polymorphic loci (P99) ranges from 0.385 to 0.769, the average number of alleles (A) ranges from 1.923 to 3.231, observed heterozygosity (Ho) ranges from 0.114 to 0.227, expected heterozygosity (He) ranges from 0.115 to 0.237. The lowest values of P99 and A are revealed in forest stands of European aspen. The lowest values of Ho and He are revealed for silver birch stands. English oak stands are characterized by the highest level of genetic variation on all indicators. Based on the DNA analysis the lowest values of the genetic diversity are revealed in aspen stands. Analyzed tree species are characterized by low levels of subdivision and differentiation. |
| first_indexed | 2026-02-08T08:01:28Z |
| format | Article |
| id | oai:ojs2.forestry-forestmelioration.org.ua:article-112 |
| institution | Forestry and Forest Melioration |
| language | Russian |
| last_indexed | 2026-02-08T08:01:28Z |
| publishDate | 2018 |
| publisher | Ukrainian Research Institute of Forestry and Forest Melioration named after G. M. Vysotsky (URIFFM) |
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| spelling | oai:ojs2.forestry-forestmelioration.org.ua:article-1122019-04-27T15:16:57Z BIODIVERSITY MONITORING OF FOREST STANDS OF DECIDUOUS SPECIES BASED ON MOLECULAR MARKING ОЦЕНКА БИОРАЗНООБРАЗИЯ ЛЕСНЫХ НАСАЖДЕНИЙ ЛИСТВЕННЫХ ДРЕВЕСНЫХ ВИДОВ НА ОСНОВЕ МОЛЕКУЛЯРНОГО МАРКИРОВАНИЯ Padutov, V. E. Kagan, D. I. Baranov, O. Yu. Ivanovskaya, S. I. Razumova, O. A. Shestibratov, K. A. European aspen, silver birch, English oak, monitoring, isoenzyme analysis, DNA analysis, genetic diversity, subdivision, differentiation осика, береза повисла, дуб звичайний, моніторинг, ізоферментний аналіз, ДНК-аналіз, генетична різноманітність, підрозділеність, диференціація Introduction Conservation and maintenance of biodiversity is promoted as an important criterion for sustainable forest management. In this regard, the improvement of methods for assessment and monitoring of biological diversity is an urgent task. The most convenient and informative tool for assessing the genetic diversity and structure of populations of living organisms is the methods of molecular genetic analysis. The use of molecular markers based on isoenzymes and polymorphic DNA fragments allows describing and differentiating genotypes of individuals with a high degree of reliability. The aim of the study is to assess the level of genetic variability, subdivision and differentiation of forest plantations of deciduous tree species. Materials and Methods The objects of the study were plantations of deciduous tree species (Populus tremula L., Betula pendula Roth., Quercus robur L.), growing on the territory of Belarus, the bordering eastern regions (Moscow and Leningrad regions of the Russian Federation), the Republic of Tatarstan. Molecular genetic analysis was done using isoenzyme and DNA-methods. The experimental material was diploid tissues of axillary buds and leaves. Homogenization, isolation and histochemical staining of the enzymes were carried out in accordance with generally accepted procedures with some modifications. DNA analysis was performed using RAPD and SSR markers. DNA isolation, polymerase chain reaction, electrophoretic fractionation, and interpretation of data were made in accordance with generally accepted procedures. Results and Conclusions To assess the level of genetic variability for each of the species studied, the parameters of genetic diversity were calculated. It was shown that the analyzed tree species are characterized by wide range of indicators of genetic diversity. According to isozyme analysis at the species level, the share of polymorphic loci (P99) ranges from 0.385 to 0.769, the average number of alleles (A) ranges from 1.923 to 3.231, observed heterozygosity (Ho) ranges from 0.114 to 0.227, expected heterozygosity (He) ranges from 0.115 to 0.237. The lowest values of P99 and A are revealed in forest stands of European aspen. The lowest values of Ho and He are revealed for silver birch stands. English oak stands are characterized by the highest level of genetic variation on all indicators. Based on the DNA analysis the lowest values of the genetic diversity are revealed in aspen stands. Analyzed tree species are characterized by low levels of subdivision and differentiation. У статті подані результати оцінки рівня генетичної мінливості, підрозділеності і диференціації лісових насаджень осики (Populus tremula L.), берези повислої (Betula pendula Roth.) і дуба звичайного (Quercus robur L.) Білорусі, прикордонних східних регіонів, Республіки Татарстан з використанням ізоферментних, RAPD- і SSR-маркерів. Показано, що проаналізовані деревні види характеризуються широким спектром показників генетичного різноманіття. За даними ізоферментного аналізу на видовому рівні в цілому частка поліморфних локусів (P99) варіює від 0,385 до 0,769, середнє число алелей (A) – від 1,923 до 3,231, наявна гетерозиготність (Ho), – від 0,114 до 0,227, очікувана гетерозиготність (He) – від 0,115 до 0,237. Найнижчі значення P99 і A виявлено в лісових насадженнях осики, Ho і He – берези повислої. Для насаджень дуба черешчатого встановлено найвищий рівень генетичної мінливості за всіма показниками. На підставі ДНК-аналізу найнижчі значення генетичної різноманітності виявлено в осикових насадженнях. Проаналізовані деревні види характеризуються низьким рівнем підрозділеності і диференціації. Ukrainian Research Institute of Forestry and Forest Melioration named after G. M. Vysotsky (URIFFM) 2018-02-08 Article Article application/pdf https://forestry-forestmelioration.org.ua/index.php/journal/article/view/112 Forestry and Forest Melioration; No. 129 (2016): Forestry and Forest Melioration; 69-75 Лісівництво і Агролісомеліорація; № 129 (2016): Лісівництво і Агролісомеліорація; 69-75 2663-4147 1026-3365 ru https://forestry-forestmelioration.org.ua/index.php/journal/article/view/112/100 |
| spellingShingle | осика береза повисла дуб звичайний моніторинг ізоферментний аналіз ДНК-аналіз генетична різноманітність підрозділеність диференціація Padutov, V. E. Kagan, D. I. Baranov, O. Yu. Ivanovskaya, S. I. Razumova, O. A. Shestibratov, K. A. ОЦЕНКА БИОРАЗНООБРАЗИЯ ЛЕСНЫХ НАСАЖДЕНИЙ ЛИСТВЕННЫХ ДРЕВЕСНЫХ ВИДОВ НА ОСНОВЕ МОЛЕКУЛЯРНОГО МАРКИРОВАНИЯ |
| title | ОЦЕНКА БИОРАЗНООБРАЗИЯ ЛЕСНЫХ НАСАЖДЕНИЙ ЛИСТВЕННЫХ ДРЕВЕСНЫХ ВИДОВ НА ОСНОВЕ МОЛЕКУЛЯРНОГО МАРКИРОВАНИЯ |
| title_alt | BIODIVERSITY MONITORING OF FOREST STANDS OF DECIDUOUS SPECIES BASED ON MOLECULAR MARKING |
| title_full | ОЦЕНКА БИОРАЗНООБРАЗИЯ ЛЕСНЫХ НАСАЖДЕНИЙ ЛИСТВЕННЫХ ДРЕВЕСНЫХ ВИДОВ НА ОСНОВЕ МОЛЕКУЛЯРНОГО МАРКИРОВАНИЯ |
| title_fullStr | ОЦЕНКА БИОРАЗНООБРАЗИЯ ЛЕСНЫХ НАСАЖДЕНИЙ ЛИСТВЕННЫХ ДРЕВЕСНЫХ ВИДОВ НА ОСНОВЕ МОЛЕКУЛЯРНОГО МАРКИРОВАНИЯ |
| title_full_unstemmed | ОЦЕНКА БИОРАЗНООБРАЗИЯ ЛЕСНЫХ НАСАЖДЕНИЙ ЛИСТВЕННЫХ ДРЕВЕСНЫХ ВИДОВ НА ОСНОВЕ МОЛЕКУЛЯРНОГО МАРКИРОВАНИЯ |
| title_short | ОЦЕНКА БИОРАЗНООБРАЗИЯ ЛЕСНЫХ НАСАЖДЕНИЙ ЛИСТВЕННЫХ ДРЕВЕСНЫХ ВИДОВ НА ОСНОВЕ МОЛЕКУЛЯРНОГО МАРКИРОВАНИЯ |
| title_sort | оценка биоразнообразия лесных насаждений лиственных древесных видов на основе молекулярного маркирования |
| topic | осика береза повисла дуб звичайний моніторинг ізоферментний аналіз ДНК-аналіз генетична різноманітність підрозділеність диференціація |
| topic_facet | European aspen silver birch English oak monitoring isoenzyme analysis DNA analysis genetic diversity subdivision differentiation осика береза повисла дуб звичайний моніторинг ізоферментний аналіз ДНК-аналіз генетична різноманітність підрозділеність диференціація |
| url | https://forestry-forestmelioration.org.ua/index.php/journal/article/view/112 |
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