Зміни в грибних угрупованнях, пов’язаних з кореневою системою дерев Pinus sylvestris, уражених кореневими гнилями
Introduction Scots pine (Pinus sylvestris) covers large areas in European regions with significant economic importance to Ukrainian forest industry. Root rot caused by the wood-decay fungus Heterobasidion annosum damages both below- and above-ground parts of Scots pines. The disease progress is like...
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| Datum: | 2023 |
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| Sprache: | Englisch |
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Ukrainian Research Institute of Forestry and Forest Melioration named after G. M. Vysotsky (URIFFM)
2023
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| Online Zugang: | https://forestry-forestmelioration.org.ua/index.php/journal/article/view/377 |
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Forestry and Forest Melioration| _version_ | 1856543473364107264 |
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| author | Davydenko , K. V. Ustsky, I. M. |
| author_facet | Davydenko , K. V. Ustsky, I. M. |
| author_sort | Davydenko , K. V. |
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| collection | OJS |
| datestamp_date | 2023-09-04T11:54:54Z |
| description | Introduction
Scots pine (Pinus sylvestris) covers large areas in European regions with significant economic importance to Ukrainian forest industry. Root rot caused by the wood-decay fungus Heterobasidion annosum damages both below- and above-ground parts of Scots pines. The disease progress is likely to be affected by reshaping in the forest such as soil properties, vegetation composition, and tree age. These changes are apparently followed up by paralleled shifts in fungal community composition on forest soil with potential feedback on ecosystem functioning.
The objective of the study was to screen fungal groups associated with the root system of P. sylvestris in stands affected by H.annosum s.s. to better understand the pathogenesis and development of root rot infection, as well as to recognize whether root size and disease severity affect diversity of fungi of the root system in the forest-steppe conditions of Ukraine. The additional object was to study other resident microflora of P. sylvestris root infested by H.annosum s.s. to find out whether the H. annosum s.s. impacts the overall diversity of other fungi.
Materials and Methods
The field study was carried out in 2018–2020. Field study sites were pure pine forest stands located in Kharkiv region (compartment 126, subcompartment 7, tract Bugri, Kharkiv Forest Research Station). Wood core and root samples from P. sylvestris were collected from the five infected (50–100 m apart from each other) and five non-infected trees (up to 500 m apart from the infested area and 50–100 m apart from each other). Wood and root samples were used for fungal culturing and direct sequencing using ITS1F and ITS4 primers.
Results
In the present study, we tried to evaluate fungal communities across diseased Pinus sylvestris stands and investigated correlations between taxonomic composition and forest health. Not surprisingly, root rot infestation had a significant effect on root-associated fungal abundance and diversity. During disease development, the root-associated fungal community shifted in composition from dominance by saprotrophic fungi to ectomycorrhizal and pathogenic fungal species. Our results suggested that maintenance of functional diversity in the root-associated fungal community may sustain long-term forest health or even root rot resistance to some extent by retaining a capacity for symbiosis-driven recycling of organic nutrients; however, this hypothesis is necessary to carefully examine and prove further.
Conclusions
Fungal culturing from 10 surface-sterilized wood cores resulted in 21 fungal cultures, 2.1 per wood segment. Direct sequencing from 40 surface-sterilized segments of lateral roots resulted in 247 fungal sequences or 6.2 per root segment on average. The most dominant fungi from the infested trees of Pinus sylvestris were Dactylonectria macrodidyma (4.98%), Acremonium sp (4.52%), Cladosporium cladosporioides (4.07%) from Ascomycota and Heterobasidion annosum s.s. (4.07%) from Basidiomycota, while for non-infested group Unidentified Ascomycota175244 (13.19%), Penicillium spinulosum (9.89%), Acremonium sp. (8.79%), Bionectriaceae sp. (8.79%) were the most common.
2 Figs., 2 Tables, 32 Refs. |
| first_indexed | 2026-02-08T08:03:47Z |
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| id | oai:ojs2.forestry-forestmelioration.org.ua:article-377 |
| institution | Forestry and Forest Melioration |
| language | English |
| last_indexed | 2026-02-08T08:03:47Z |
| publishDate | 2023 |
| publisher | Ukrainian Research Institute of Forestry and Forest Melioration named after G. M. Vysotsky (URIFFM) |
| record_format | ojs |
| spelling | oai:ojs2.forestry-forestmelioration.org.ua:article-3772023-09-04T11:54:54Z Shift in fungal communities associated with Pinus sylvestris stands affected by root rot Зміни в грибних угрупованнях, пов’язаних з кореневою системою дерев Pinus sylvestris, уражених кореневими гнилями Davydenko , K. V. Ustsky, I. M. Scots pine Heterobasidion annosum s.l. сосна звичайна Heterobasidion annosum s.l. Introduction Scots pine (Pinus sylvestris) covers large areas in European regions with significant economic importance to Ukrainian forest industry. Root rot caused by the wood-decay fungus Heterobasidion annosum damages both below- and above-ground parts of Scots pines. The disease progress is likely to be affected by reshaping in the forest such as soil properties, vegetation composition, and tree age. These changes are apparently followed up by paralleled shifts in fungal community composition on forest soil with potential feedback on ecosystem functioning. The objective of the study was to screen fungal groups associated with the root system of P. sylvestris in stands affected by H.annosum s.s. to better understand the pathogenesis and development of root rot infection, as well as to recognize whether root size and disease severity affect diversity of fungi of the root system in the forest-steppe conditions of Ukraine. The additional object was to study other resident microflora of P. sylvestris root infested by H.annosum s.s. to find out whether the H. annosum s.s. impacts the overall diversity of other fungi. Materials and Methods The field study was carried out in 2018–2020. Field study sites were pure pine forest stands located in Kharkiv region (compartment 126, subcompartment 7, tract Bugri, Kharkiv Forest Research Station). Wood core and root samples from P. sylvestris were collected from the five infected (50–100 m apart from each other) and five non-infected trees (up to 500 m apart from the infested area and 50–100 m apart from each other). Wood and root samples were used for fungal culturing and direct sequencing using ITS1F and ITS4 primers. Results In the present study, we tried to evaluate fungal communities across diseased Pinus sylvestris stands and investigated correlations between taxonomic composition and forest health. Not surprisingly, root rot infestation had a significant effect on root-associated fungal abundance and diversity. During disease development, the root-associated fungal community shifted in composition from dominance by saprotrophic fungi to ectomycorrhizal and pathogenic fungal species. Our results suggested that maintenance of functional diversity in the root-associated fungal community may sustain long-term forest health or even root rot resistance to some extent by retaining a capacity for symbiosis-driven recycling of organic nutrients; however, this hypothesis is necessary to carefully examine and prove further. Conclusions Fungal culturing from 10 surface-sterilized wood cores resulted in 21 fungal cultures, 2.1 per wood segment. Direct sequencing from 40 surface-sterilized segments of lateral roots resulted in 247 fungal sequences or 6.2 per root segment on average. The most dominant fungi from the infested trees of Pinus sylvestris were Dactylonectria macrodidyma (4.98%), Acremonium sp (4.52%), Cladosporium cladosporioides (4.07%) from Ascomycota and Heterobasidion annosum s.s. (4.07%) from Basidiomycota, while for non-infested group Unidentified Ascomycota175244 (13.19%), Penicillium spinulosum (9.89%), Acremonium sp. (8.79%), Bionectriaceae sp. (8.79%) were the most common. 2 Figs., 2 Tables, 32 Refs. Кореневі гнилі, викликані грибами Heterobasidion annosum s.l. та іншими дереворуйнівними грибами, уражують підземну й надземну частини сосни звичайної (Pinus sylvestris L.). Властивості ґрунту та рослинного покриву змінюються в часі, і ці зміни, ймовірно, також впливають на розвиток хвороби й викликають зміни грибних угруповань лісового ґрунту та асоційованих із кореневою системою грибів. У цьому дослідженні ми спробували оцінити видовий і функціональний склад грибних угруповань у насадженнях P. sylvestris, уражених кореневими гнилями, й дослідили кореляції між таксономічним складом грибів кореневої системи і фізіологічним станом дерев. Ураження кореневими гнилями значною мірою впливало на чисельність і різноманітність грибів. Під час розвитку захворювання в складі грибних угруповань виникли зміни від домінування сапротрофних грибів до панування ектомікоризних і патогенних видів грибів. Наші результати дають змогу зробити припущення, що збереження біологічного різноманіття в грибному угрупованні кореневої системи може певною мірою впливати на підтримання нормального фізіологічного стану дерев або навіть на стійкість до кореневої гнилі, причому зберігається здатність грибів до переробки органічних поживних речовин, однак для підтвердження цієї гіпотези необхідні подальші дослідження. Ukrainian Research Institute of Forestry and Forest Melioration named after G. M. Vysotsky (URIFFM) 2023-06-29 Article Article application/pdf https://forestry-forestmelioration.org.ua/index.php/journal/article/view/377 10.33220/1026-3365.142.2023.136 Forestry and Forest Melioration; No. 142 (2023): Forestry and Forest Melioration; 136-146 Лісівництво і Агролісомеліорація; № 142 (2023): Лісівництво і Агролісомеліорація; 136-146 2663-4147 1026-3365 10.33220/1026-3365.142.2023 en https://forestry-forestmelioration.org.ua/index.php/journal/article/view/377/345 http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | сосна звичайна Heterobasidion annosum s.l. Davydenko , K. V. Ustsky, I. M. Зміни в грибних угрупованнях, пов’язаних з кореневою системою дерев Pinus sylvestris, уражених кореневими гнилями |
| title | Зміни в грибних угрупованнях, пов’язаних з кореневою системою дерев Pinus sylvestris, уражених кореневими гнилями |
| title_alt | Shift in fungal communities associated with Pinus sylvestris stands affected by root rot |
| title_full | Зміни в грибних угрупованнях, пов’язаних з кореневою системою дерев Pinus sylvestris, уражених кореневими гнилями |
| title_fullStr | Зміни в грибних угрупованнях, пов’язаних з кореневою системою дерев Pinus sylvestris, уражених кореневими гнилями |
| title_full_unstemmed | Зміни в грибних угрупованнях, пов’язаних з кореневою системою дерев Pinus sylvestris, уражених кореневими гнилями |
| title_short | Зміни в грибних угрупованнях, пов’язаних з кореневою системою дерев Pinus sylvestris, уражених кореневими гнилями |
| title_sort | зміни в грибних угрупованнях, пов’язаних з кореневою системою дерев pinus sylvestris, уражених кореневими гнилями |
| topic | сосна звичайна Heterobasidion annosum s.l. |
| topic_facet | Scots pine Heterobasidion annosum s.l. сосна звичайна Heterobasidion annosum s.l. |
| url | https://forestry-forestmelioration.org.ua/index.php/journal/article/view/377 |
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