ТЕПЛО- ТА МАСООБМІН В АПАРАТАХ ОТРИМАННЯ БІЛКА НА ОСНОВІ ВОДЕНЬОКИСЛЮЮЧИХ БАКТЕРІЙ

• Date: 2026
• Main Authors: Troshen'kin, B.A., Kravchenko, O.V., Troshen'kin, V.B.
• Format: Article
• Language: Ukrainian
• Published: Institute of Engineering Thermophysics of NAS of Ukraine 2026
• Online Access: https://ihe.nas.gov.ua/index.php/journal/article/view/661
• Journal Title: Thermophysics and Thermal Power Engineering

Global economic development trends urgently require the transition of agricultural food production to industrial scale. Numerous patents recently obtained confirm that many countries are interested in protein production processes, particularly those based on hydrogen-oxidizing bacteria. Research into this process is currently in the laboratory experimentation and preliminary design stages for pilot plants. This paper examines the current state of development in protein production using hydrogen-oxidizing bacteria. To identify deficiencies in the biomass cultivation process, a pilot plant was created and a series of studies were conducted. The results were compared with existing data. Based on the experimental results, the key principles of the biomass production process were clarified, and the reliability of individual components of the laboratory setup was verified. A comprehensive analysis of the resulting biomass, conducted in I. Mechnikov Institute of Microbiology and Immunology (Kharkiv, Ukraine) established that, during continuous cultivation, protein synthesis by the Alkaligenes eutropha Z1 bacterium is stable, accounting for 72-74 % of the resulting biomass, consistent with published data. The content of lipid, carbohydrate components, and nucleic acids is within normal limits. The presence of other species in the biomass does not exceed permissible levels. Thus, it has been proven that the individual components and the entire system operate reliably. Based on the results of experimental studies of the process, it was recommended to change the equipment design of the stand – to dissolve hydrogen in the cultural liquid before feeding it to the fermenter, and to use film devices as fermenters with stirrers. A calculation sequence for heat and mass transfer in a film fermenter was developed. It has been shown that the conversion of protein production from agricultural methods to industrial ones will yield significant economic benefits and improve the environment by reducing harmful emissions into the atmosphere.