IOON ONE MED Device Generates Singlet Hydrogen and Active Chlorine to Exhibit Antimicrobial Activity – an Experimental Study

• Дата: 2024
• Автори: Manuilov, M.B., Martynov, A.V., Sklyar, N.I., Minukhin, V.V., Biryukov, M.S., Manuilov, A.M., Мануйлов, М.Б., Мартинов, А.В., Скляр, Н.І., Мінухін, В.В., Бірюков, М.С., Мануйлов, А.М.
• Формат: Стаття
• Мова: English
• Опубліковано: PH "Akademperiodyka" of the NAS of Ukraine 2024
• Онлайн доступ: https://ojs.microbiolj.org.ua/index.php/mj/article/view/171
• Назва журналу: Microbiological Journal

The aim of the study was to investigate the in vitro antimicrobial activity against museum and circulating strains of microorganisms and fungi of an aqueous solution generated by the IOON ONE MED device containing atomic hydrogen and atomic (active) chlorine at a concentration of 1 mg/L as active substances and in vivo on a standardized model of purulent peritonitis in two variants – prophylactic (neutralization of bacteria) and therapeutic (effect on advanced infection). Methods. Microbiological methods were used to study the antimicrobial activity of new substances in vitro on museum and circulating strains of microorganisms, as well as fungi. The ability of the generated solution to exhibit antibiofilm effects on intubation tubes infected with resistant microorganisms was studied separately. In vivo study of the efficacy of the generated solution on a model of purulent peritonitis in mice was also conducted in two ways – by using the solution as a prophylactic agent (mixing live bacteria with the solution and then immediately testing the ability of bacteria to induce peritonitis) and by therapeutic effect by injecting the solution 3 hours after infection of mice and determining the number of surviving mice. Results. The results showed that the solution produced by the device worked as well as 0.05 % chlorhexidine and 3 % hydrogen peroxide. From the in vivo studies, it can be concluded that the drug under investigation does not cause acute toxicity: the injection of 1 mL of the generated solution intraperitoneally did not lead to the death of any mice. It was found that mixing 1:1 of the solution generated by the IOON ONE MED device with a suspension of P. aeruginosa #2261 and subsequent intraperitoneal injection into mice ensured 100 % survival of animals compared to 100 % mortality in the control group. When the mice were injected with the intraperitoneal solution three hours after infection with a lethal dose of the resistant hospital strain of P. aeruginosa #2261, a 40 % survival rate was observed compared to 100% mortality in the control group. The difference between the control and experimental groups was statistically significant. Conclusions. The solution generated by the IOON ONE MED device, containing atomic hydrogen and atomic chlorine, when used in combination, has strong antiseptic properties and has promising application for surface disinfection, despite its short residence time in solution.