Effect of 3,3ʹ-Diindolylmethane in Different Solvents on Pseudomonas Aeruginosa Biofilm

• Дата: 2025
• Автори: Malanchuk, S., Mishyna, M., Kot, Yu., Dzhoraieva, S., Mozgova, Yu., Malanchuk, B., Mishyn, Yu., Маланчук, С., Мішина, М., Кот, Ю., Джораєва, С., Мозгова, Ю., Маланчук, Б., Мішин, Ю.
• Формат: Стаття
• Мова: Англійська
• Опубліковано: PH "Akademperiodyka" of the NAS of Ukraine 2025
• Онлайн доступ: https://ojs.microbiolj.org.ua/index.php/mj/article/view/337
• Назва журналу: Microbiological Journal

Today, the most common pathogens causing nosocomial infections are Gram-negative bacteria, especially Pseudomonas aeruginosa. One of the leading factors in determining the resistance of bacteria is their ability to form biofilms. One promising class of agents capable of affecting biofilms is indoles (diindolylmethane). The aim of this work was to determine the effect of diindolylmethane in different solvents on the formation of P. aeruginosa biofilm. Methods. For microbiologic examination, biological material was collected from purulent-inflammatory complicated gunshot and shrapnel wounds. The antimicrobial activity of samples containing 0.5% solution of 3,3ʹ-diindolylmethane derivatives (Ts-D5-1 – sample  1, Ts-D8-1 – 2, VE-D67-1 –3, VE-D68-1–4, VE-D71-1–5, W-014-1–6, W-015-1–7, and W-016-1–8 in dimethylsulfoxide solvent and Ts-D5-2 –9, Ts-D8-2 – 10, VE-D67-2 – 11, VE-D68-2 –12, VE-D71-2 – 13, W-014-2 – 14, W-015-2 – 15,and W-016-2 – 16 in N-methylpyrrolidone solvent) was determined. The study was carried out by the method of diffusion in agar. The biofilm formation study was performed according to the method of O'Toole. Results. Samples 14, 15, and 16 showed a high antimicrobial activity, among which sample 14 was the most efficient: the diameter of the lysis zone was 10 mm after 24 hours, 11 mm after 48 hours, and 12 mm after 72 hours. It was demonstrated that all other samples had less marked antibacterial activity, which was slightly potentiated over time. Conclusions. Sample 14 showed the highest antimicrobial activity. Moreover, the effect of potentiation of the antimicrobial activity of the solution was observed. The test solution prevented the formation of a biofilm when it was applied to the well surface, and also led to the destruction of the already formed daily biofilm of P. aeruginosa.