Phytoprotective Potential of Soybean Endophytic Microsymbionts

• Дата: 2026
• Автори: Shevchuk, N.V., Tytova, L.V., Sergiienko, V.G., Iutynska , G.O., Шевчук, Н.В., Титова, Л.В., Сергієнко, В.Г., Іутинська, Г.О.
• Формат: Стаття
• Мова: Англійська
• Опубліковано: PH "Akademperiodyka" of the NAS of Ukraine 2026
• Онлайн доступ: https://ojs.microbiolj.org.ua/index.php/mj/article/view/382
• Назва журналу: Microbiological Journal

The phytosphere microbiome plays a key role in maintaining plant health under stressful environmental conditions. Endophytic microorganisms are particularly important due to their different probiotic functions. The aim of the work was to study the effect of endophytic bacteria on phytopathogenic microorganisms, soybean resistance to biotic stresses and its productivity. Methods. Microbiological (cultivation of microorganisms to obtain microbial bioformulations, study of the germination of phytopathogenic micromycetes spores by light microscopy, study of the antagonistic activity of endophytes against phytopathogenic bacteria by the agar block method), biochemical (determination of siderophore synthesizing activity), laboratory-vegetation (research of the effect of endophytes on soybean resistance to phytopathogens under artificial infection), field (soybean plant cultivation, determination of their resistance to diseases under natural infectious background and productivity), and statistical (the significance of the differences determination). The following microorganisms were studied in the work: endophytic bacteria, soybean nodule bacteria, phytopathogenic bacteria, and fungi. Results. Endophytic bacteria isolated from soybean nodules showed antagonism to pathogens of legume and vegetable crops (growth retardation zone diameter from 13.4 to 20 mm) and inhibited the germination of the phytopathogenic fungi spores Fusarium oxysporum 080721 and Alternaria alternata 150721. The ability to synthesize siderophores was detected in all strains, among them Paenibacillus sp. 1, Bacillus velezensis IMV B-8134, and Pseudomonas sp. 6 were the most active, with 75.9%, 57.7%, and 78.3%, respectively. Under soybean seeds inoculation with RyzobinK + B. velezensis IMV B-8134, followed by spraying the leaves with the metabolites of this strain, the protective effect was observed against F. oxysporum 080721 (70.1%), A. alternata 150721 (50.9%), and P. savastanoi pv. glycinea UCM B-1150 (61.9%). Under field experiments on a natural infectious background, inoculation of soybean seeds of different varieties with the composition of RyzobinK together with endophytic strains resulted in protection against alternariosis ranging from 47.5 to 92%. Soybean productivity was also increased. Conclusions. Selected strains of endophytic bacteria are promising for the development of multifunctional microbial preparations for new adaptive biotechnologies capable for maintaining plant health and helping to prevent crop losses under biotic stresses. The use of endophytic bacteria in soybean cultivation technologies will increase plant resistance to pathogens and yield under adverse phytosanitary environmental conditions.