Phylogenetic Analysis of Phytopathogenic Bacteria Affecting Walnuts (Juglans sp.) in Ukraine
Walnuts (Juglans sp.) are affected by a wide range of bacterial pathogens belonging to different taxa, and their accurate identification is necessary for understanding both the etiology of diseases and the development of effective control measures. Phylogenetic analysis based on the analysis of the...
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| Date: | 2026 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Published: |
PH "Akademperiodyka" of the NAS of Ukraine
2026
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| Online Access: | https://ojs.microbiolj.org.ua/index.php/mj/article/view/427 |
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| Journal Title: | Microbiological Journal |
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Microbiological Journal| Summary: | Walnuts (Juglans sp.) are affected by a wide range of bacterial pathogens belonging to different taxa, and their accurate identification is necessary for understanding both the etiology of diseases and the development of effective control measures. Phylogenetic analysis based on the analysis of the 16S rRNA gene is widely used in bacterial taxonomy. However, taxonomic studies of pathogens of bacterial diseases of walnuts in Ukraine are poorly known. The aim of this study was to determine the taxonomic position of phytopathogenic bacteria isolated from walnuts (Juglans sp.) in Ukraine using comparative analysis of nucleotide sequences of the 16S rRNA gene. Methods. The 16S rRNA gene was amplified by universal primers pA and pH. Sequence assembly, editing, and alignment were performed using Multalin and Sequence Manipulation Suite software. The rate of the 16S rRNA gene nucleotide sequences similarity of isolated strains with homology nucleotide sequences of typical strains located in GenBank was established using the BLASTN program. Phylogenetic relatedness was established and graphically displayed using the MEGA program. Results. Phylogenetic analysis of 16S rRNA gene sequences showed that isolated Agrobacterium spp. strains have the highest percentage of nucleotide sequence similarity (99.4–99.9%) with Agrobacterium tumefaciens LGM 196. Nucleotide sequences of the 16S rRNA gene of isolated Xanthomonas spp. strains are 98.51–99.65% related to the identical sequence of Xanthomonas arboricola pv. juglandis NCPPB 411. Pseudomonas spp. isolates are phylogenetically related (98.31–99.51%) to Pseudomonas syringae pv. syringae UCM B-1027ᵀ and P. syringae pv. syringae ATCC 19310. Conclusions. The high level of similarity of the 16S rRNA gene sequences isolated from the type strains confirms their taxonomic affiliation to these taxa and emphasizes the importance of phylogenetic analysis in the taxonomy of walnut pathogenic bacteria. Comparative analysis of the 16S rRNA gene sequences in combination with phenotypic characteristics confirms that the bacteria isolated from walnut belong to the species Agrobacterium tumefaciens, Xanthomonas arboricola, and Pseudomonas syringae. |
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