Вміст фенольних сполук у рослинній сировині Cichorium intybus L., Lamium purpureum L. та Viscum album l.
Objective – to evaluate the antioxidant potential of ethanol extracts of wild selected plants in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine through the determination of phenolic compounds. Material and methods. In this study used dried raw of Cichorium intybus L., Lam...
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| Date: | 2019 |
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| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
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M.M. Gryshko National Botanical Garden of the NAS of Ukraine
2019
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| author | Vergun, O.M. Grygorieva, O.V. Brindza, J. Shymanska, O.V. Rakhmetov, D.B. Horčinová-Sedlačková, V. Korablova, O.A. Fishchenko, V.V. Ivanišová, E. |
| author_facet | Vergun, O.M. Grygorieva, O.V. Brindza, J. Shymanska, O.V. Rakhmetov, D.B. Horčinová-Sedlačková, V. Korablova, O.A. Fishchenko, V.V. Ivanišová, E. |
| author_sort | Vergun, O.M. |
| baseUrl_str | https://www.plantintroduction.org/index.php/pi/oai |
| collection | OJS |
| datestamp_date | 2019-11-11T08:16:08Z |
| description | Objective – to evaluate the antioxidant potential of ethanol extracts of wild selected plants in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine through the determination of phenolic compounds.
Material and methods. In this study used dried raw of Cichorium intybus L., Lamium purpureum L. and Viscum album L. Plants of C. intybus and L. purpureum harvested from natural flora of the M.M. Gryshko National Botanical Garden of the NAS of Ukraine. V. album collected from crown trees of Tilia cordata Mill. 0.2 g of dried plant raw material was extracted with 20 mL of 80 % ethanol for 2 hours. The total polyphenol content of extracts was measured by the method described by Singleton and Rossi (1965) using Folin-Chiocalteu reagent and results were expressed in mg of gallic acid equivalent per one gram of dry matter (mg GAE/g). Determination of total flavonoids content was conducted using the modified method described in Shafii et al. (2017) and results expressed in mg quercetin equivalent per one gram of dry matter (mg QE/g). Detection of total phenolic acids content of extracts was carried out using the method described in Farmakopea Polska (1999) and results expressed in mg caffeic acid per one gram of dry matter (mg CAE/g). The antioxidant activity of samples was measured using 2.2-diphenyl-1-picrylhydrazyl (DPPH method) according to Sanchez-Moreno et al. (1998). Also, the antioxidant activity of extracts was determined by the phospho-molybdenum method described by Prieto et al. (1999) with slight modifications. Results of these parameters expressed in mg Trolox equivalent per one gram of dry matter (mg TE/g). Experimental data were evaluated by using Excel 2010. Correlation analysis performed using Pearson’s criterion.
Results. The total content of polyphenol compounds for C. intybus, L. purpureum and V. album was 33.91, 34.61 and 31.28 mg GAE/g, respectively, the total content of flavonoids for C. intybus, L. purpureum and V. album – 26.29, 28.89 and 25.10 mg QE/g, the total content of phenolic acids – 4.56, 4.87 and 4.07 mg CAE/g, antioxidant activity of extracts by DPPH method was 8.35, 7.66 and 8.55 mg Trolox Equivalent/g, respectively, antioxidant activity by phosphomolybdenum method – 93.01, 142.62 and 9.31 mg Trolox Equivalent/g. Between the accumulation of polyphenol compounds and antioxidant activity of extracts found a strong positive correlation.
Conclusions. Wild plants of C. intybus, L. purpureum and V. album in M.M. Gryshko National Botanical Garden of the NAS of Ukraine accumulated polyphenol compounds with high antioxidant activity. Obtained data demonstrated that these plant species can be a potential source of natural antioxidants that can be used in the different pharmacological investigations. It is important to a branch of biological science to investigate biochemical properties of not cultivated plants only but wild plants also to identify new sources of biologically active compounds. |
| doi_str_mv | 10.5281/zenodo.3404149 |
| first_indexed | 2025-07-17T12:53:21Z |
| format | Article |
| fulltext |
87ISSN 16056574. Інтродукція рослин, 2019, № 3: 87—96
https://doi.org/ 10.5281/zenodo.3404149
UDC 581.192
O.M. VERGUN 1, O.V. GRYGORIEVA 1, J. BRINDZA 2,
O.V. SHYMANSKA 1, D.B. RAKHMETOV 1, V. HORA INOVB SEDLAA KOVB 2,
O.A. KORABLOVA 1, V.V. FISHCHENKO 1, E. IVANIC OVB 2
1 M.M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine
Ukraine, 01014 Kyiv, Timiryazevska str., 1
2 Slovak Agricultural University in Nitra
Slovak Republic, 94976 Nitra, Trieda Andreja Hlinku, 2
en_vergun@ukr.net
CONTENT OF PHENOLIC COMPOUNDS
IN PLANT RAW OF CICHORIUM INTYBUS L.,
LAMIUM PURPUREUM L. AND VISCUM ALBUM L.
Objective — to evaluate the antioxidant potential of ethanol extracts of wild selected plants in conditions of M.M. Gryshko Natio
nal Botanical Garden of the NAS of Ukraine through the determination of phenolic compounds.
Material and methods. In this study used dried raw of Cichorium intybus L., Lamium purpureum L. and Viscum album L.
Plants of C. intybus and L. purpureum harvested from natural flora of the M.M. Gryshko National Botanical Garden of the NAS
of Ukraine. V. album collected from crown trees of Tilia cordata Mill. 0.2 g of dried plant raw material was extracted with 20 mL of
80 % ethanol for 2 hours. The total polyphenol content of extracts was measured by the method described by Singleton and
Rossi (1965) using FolinChiocalteu reagent and results were expressed in mg of gallic acid equivalent per one gram of dry mat
ter (mg GAE/g). Determination of total flavonoids content was conducted using the modified method described in Shafii et al.
(2017) and results expressed in mg quercetin equivalent per one gram of dry matter (mg QE/g). Detection of total phenolic acids
content of extracts was carried out using the method described in Farmakopea Polska (1999) and results expressed in mg caffeic
acid per one gram of dry matter (mg CAE/g). The antioxidant activity of samples was measured using 2.2diphenyl1picrylhy
drazyl (DPPH method) according to SanchezMoreno et al. (1998). Also, the antioxidant activity of extracts was determined by
the phosphomolybdenum method described by Prieto et al. (1999) with slight modifications. Results of these parameters ex
pressed in mg Trolox equivalent per one gram of dry matter (mg TE/g). Experimental data were evaluated by using Excel 2010.
Correlation analysis performed using Pearson’s criterion.
Results. The total content of polyphenol compounds for C. intybus, L. purpureum and V. album was 33.91, 34.61 and
31.28 mg GAE/g, respectively, the total content of flavonoids for C. intybus, L. purpureum and V. album — 26.29, 28.89 and
25.10 mg QE/g, the total content of phenolic acids — 4.56, 4.87 and 4.07 mg CAE/g, antioxidant activity of extracts by DPPH
method was 8.35, 7.66 and 8.55 mg Trolox Equivalent/g, respectively, antioxidant activity by phosphomolybdenum method —
93.01, 142.62 and 9.31 mg Trolox Equivalent/g. Between the accumulation of polyphenol compounds and antioxidant activity
of extracts found a strong positive correlation.
Conclusions. Wild plants of C. intybus, L. purpureum and V. album in M.M. Gryshko National Botanical Garden of the NAS
of Ukraine accumulated polyphenol compounds with high antioxidant activity. Obtained data demonstrated that these plant spe
cies can be a potential source of natural antioxidants that can be used in the different pharmacological investigations. It is im
portant to a branch of biological science to investigate biochemical properties of not cultivated plants only but wild plants also to
identify new sources of biologically active compounds.
Key words: Cichorium intybus, Lamium purpureum, Viscum album, polyphenols, flavonoids, phenolic acids, antioxidant
activity.
© O.M. VERGUN, O.V. GRYGORIEVA,
J. BRINDZA, O.V. SHYMANSKA, D.B. RAKHMETOV,
V. HOR D INOVE SEDLA D KOVE , O.A. KORABLOVA,
V.V. FISHCHENKO, E. IVANI F OVE , 2019
88 ISSN 16056574. Plant introduction, 2019, № 3
O.M. Vergun, O.V. Grygorieva, J. Brindza, O.V. Shymanska, D.B. Rakhmetov, V. HorG inovH SedlaG kovH , O.A. Korablova...
Natural compounds isolated from plant raw ma
terial possess multiple biological activities such as
antioxidant. Among biologically active compounds
that cause the antioxidant activity can be high
lighted polyphenol compounds with useful thera
peutical properties [31]. Natural polyphenols are
found in various plants including wild and weeds
and play an important role in human life. The most
investigated plant species of this direction related
to traditional medicinal, fruits, aromatic, and food
plants [29]. In present study considered results of
a study of polyphenol compounds accumulation
and antioxidant activity of three wild growing plant
species, which are interesting objects in the rela
tion of biochemical properties.
Cichorium intybus L. (chicory) belongs to Aster
aceae Bercht. & J. Presl family and widely distrib
uted in Asia, Europe, South Africa, etc. Plant raw
material of this plant is an important source of
biologically active compounds (Table 1). This
plant used in the traditional medicine of many
countries [17]. It is a perennial, a deeprooting
herb that usually described as wild plant [22]. In
addition, chicory is known as a vegetable, fresh or
cooked, while the ground and roasted roots are
widely used for blending with coffee powder. Some
varieties of this plant have cultivated in Italy [23].
Lamium purpureum L. (red deadnettle) is a
member of the Lamiaceae Martinov family and
grows in Europe, Asia, Africa, etc. The last study
showed that this plant can be used as control
agents of stored food products due to inhibition
action in relation to red flour beetle Tribolium
castaneum Herbst [3].
Viscum album L. (mistletoe) belonging to Lo
ranthaceae Juss. family and used for the treatment
of many diseases as folk medicine in Europe and
Northern Asian countries. This plant grows as a
semiparasitic on different trees and shrubs [38].
Biological activity and qualitative content of
biologically active compounds in the raw of se
lected plans represented below (Table 1 and 2).
Material and methods
Plant materials
In this study used dried raw of Cichorium intybus,
Lamium purpureum and Viscum album. Plants of
C. intybus and L. purpureum harvested from natural
flora of the M.M. Gryshko National Botanical Gar
den of the NAS of Ukraine in the flowering stage.
V. album collected from trees of Tilia cordata Mill.
Chemicals
All chemicals were analytical grade and were pur
chased from Reachem (Slovakia) and Sigma Ald
rich (USA).
Sample preparation
0.2 g of dried plant raw material was extracted with
20 ml of 80 % ethanol for 2 hours. After centrifu
gation at 4000 g (Rotofix 32 A, Hettich, Germany)
for 10 min, the supernatant was used for the next
measurements: antioxidant activity, polyphenols,
and flavonoids.
Total polyphenol content
Total polyphenol content (TPC) of extracts was
measured by the method of Singleton and Rossi
(1965) using FolinChiocalteu reagent [49]. 0.1 ml
Table 1. Biologically active compounds in plant raw material of Cichorium intybus L.,
Lamium purpureum L. and Viscum album L.
Species Biologically active compounds in raw References
Cichorium intybus Alkaloids, inulin, sesquiterpene lactones, coumarins,
vitamins, unsaturated sterols, flavonoids, tannins, cichoric
acid, glycosides, anthocyanins, etc.
[1]; [7]; [9]; [13]; [33—36]
Lamium purpureum Vitamins, phenylethanoid glycosides [18]; [24]
Viscum album Lectins, viscotoxins, alkaloids (doesn’t contain typical
alkaloids), amine alkaloids, triterpenes, flavanone glyco
sides, flavanones
[6]; [16]; [30]
89ISSN 16056574. Інтродукція рослин, 2019, № 3
Content of phenolic compounds in plant raw of Cichorium intybus L., Lamium purpureum L. and Viscum album L.
of each sample extract was mixed with 0.1 ml of
the FolinChiocalteu reagent, 1 ml of 20 % (w/v)
sodium carbonate and 8.8 ml of distilled water. Af
ter 30 min in darkness, the absorbance at 700 nm
was measured using the spectrophotometer Jen
way (6405 UV/Vis, England). Gallic acid (25—250
mg/L; R2 = 0.996) was used as the standard and
the results were expressed in mg/g gallic acid equi
valents.
Total flavonoid content
Determination of total flavonoids content (TFC)
was conducted using the modified method de
scribed in Shafii et al. (2017) [47]. 0.5 ml of sam
ple extract was mixed with 0.1 ml of 10 % (w/v)
ethanolic solution of aluminum chloride, 0.1 ml
of 1 M sodium acetate and 4.3 ml of distilled wa
ter. After 30 min. in darkness the absorbance at
415 nm was measured using the spectrophotome
ter Jenway (6405 UV/Vis, England). Quercetin
(0.01—0.5 mg/L; R2=0.997) was used as the stand
ard and the results were expressed in mg/g querce
tin equivalents.
Total phenolic acid content
Determination total phenolic acids content (TPA)
of extracts was carried out using the method de
scribed in Farmakopea Polska (1999) [19]. 0.5 ml
of sample extract was mixed with 0.5 ml of 0.5 M
hydrochloric acid, 0.5 ml Arnova reagent, 0.5 ml
of 1 M sodium hydroxide (w/v) and 0.5 ml of dis
tilled water. Absorbance at 490 nm was measured
using the spectrophotometer Jenway (6405 UV/
Vis, England). Caffeic acid (1—200 mg/L, R2 =
0.999) was used as a standard and the results were
expressed in mg/g caffeic acid equivalents.
Antioxidant activity by DPPH method
The radical scavenging activity of samples was meas
ured using 2.2diphenyl1picrylhydrazyl (DPPH)
according to SanchezMoreno et al., 1998 [46]. The
extracts (0.5 ml) were mixed with 3.6 ml of radical
solution (0.025 g of DPPH in 100 ml ethanol). The
absorbance of the sample extract was determined us
ing the spectrophotometer Jenway (6405 UV/Vis,
England) at 515 nm. Trolox (6hydroxy2,5,7,8tet ra
methylchroman2carboxylic acid) (10—100 mg/L;
R2 = 0.988) was used as the standard and the results
were expressed in mg/g Trolox equivalents.
Antioxidant activity by phosphomolybdenum
method
Reducing power of extracts was determined by the
phosphomolybdenum (PhMo) method of Prieto
et al. (1999) with slight modifications [44]. The mix
ture of sample (1 ml), monopotassium phosphate
Table 2. Biological activity of plant raw material of Cichorium intybus L., Lamium purpureum L. and Viscum album L.
Species Biological activity of raw
Use in traditional
and folk medicine
References
Cichorium intybus Antihepatotoxic, antidiabetic, an ti
bacterial, antiinflammatory, hyper
glycaemic, antiulcerogenic, antioxi
dant, antiallergic, anthelmintic
Fever, diarrhoea, jaundice,
gallstones, AIDS, cancer, in
somnia, splenitis, tachycar
dia, bruises
[1]; [2]; [4]; [8]; [14];
[26]; [28]; [36]; [41];
[42]; [45]
Lamium
purpureum
Insecticidal, diuretic, antiinflam ma
tory, antidiarrheal, astringent, ex pec
torant, antirheumatic, haemostatic,
antioxidant, antimicrobial
Disorders: trauma, fracture,
paralysis, hypertension, uter
ine hemorrhage
[3]; [11]; [12]; [51]; [53];
[54]
Viscum album Antioxidant, antitumor, anticancer,
cytotoxic, antimicrobial, cytostatic,
antihypertensive, antinociceptive, im
munomodulatory, sedative, anti psy
cho tic
Hypertension, diabetes, ar th
r osis, cancer, epilepsy, head
ache
[5]; [16]; [27]; [37—39];
[40]; [43]
90 ISSN 16056574. Plant introduction, 2019, № 3
O.M. Vergun, O.V. Grygorieva, J. Brindza, O.V. Shymanska, D.B. Rakhmetov, V. HorI inovJ SedlaI kovJ , O.A. Korablova...
(2.8 ml, 0.1 M), sulfuric acid (6 ml, 1 M), ammoni
um heptamolyb date (0.4 ml, 0.1 M) and distilled
water (0.8 ml) incubated at 90 °C for 120 min, then
rapidly cooled and detected by monitoring absorb
ance at 700 nm using the spectrophotometer Jenway
(6405 UV/Vis, England). Trolox (10—1000 mg/L;
R2 = 0.998) was used as the standard and the results
were expressed in mg/g Trolox equivalents.
Experimental data were evaluated by using Ex
cel 2010. Data were analyzed with ANOVA test
and differences between means were compared
through the Tukey—Kramer test (K = 0.05); signi
ficance level at p < 0.05. Mean values of three rep
licates and standard deviation are given in Fig. 1, 2.
Correlation analysis performed using Pearson’s cri
terion.
Results and discussions
Study of antioxidant activity of plant raw and con
tent of polyphenol compounds is a very popular
branch of modern biology [29]. There are numer
ous investigations of antioxidant potential of dif
ferent groups of plants such as medicinal, food,
fruits, energetic, invasive, etc. [10; 15; 21; 48; 52].
Investigation of biological activity of wild plants
that related to the weeds or even parasitic plants
but can have useful properties can be an important
direction of biology to find new sources of biolog
ically active compounds.
In our study, we used ethanol extracts that are
mostly used in traditional medicine along with
water extracts [31]. The total content of polyphe
nol compounds for three investigated plants C. in
ty bus, L. purpureum, and V. album was 33.91, 34.61
and 31.28 mg GAE/g respectively (Fig. 1). As re
ported Abbas et al. (2015), phenolic content in the
C. intybus extracts was 85.0 mg GAE/g [1]. Study
of Jancic et al. (2017) showed that TPC in extracts
of wild and cultivated chicory were 1.05—3.73 and
0.65—0.82 mg GAE/g respectively [25]. Malik et
al. (2017) demonstrated that TPC was 21.01 mg
GAE/g for wild plants of chicory [32]. Innocenti
et al. (2005) studied polyphenol complex of alco
hol extracts of C. intybus and found that cichoric
acid the most abundant compound among the to
tal phenols [23].
In the study of GrujiL et al. (2017) represented
that the total content of polyphenols for ethanol
extracts of L. purpureum was 89.23 mg GAE/g [20].
Our result was 2.5 times less comparing with this
study. In the report of Kang (2016) indicated that
the content of polyphenols was 60.46 mg CAE/g
(catechin equivalents) [27]. TahiroviL and BaM iL
(2017) determined the TPC for leaves and stems of
C
on
te
n
t
of
p
ol
yp
h
en
ol
s
(m
g
G
A
E
/g
)
an
d
fl
av
on
oi
ds
(
m
g
Q
E
/g
)
35
30
25
20
15
10
5
0
C
on
te
n
t
of
p
h
en
ol
ic
a
ci
ds
,
m
g
C
A
E
/g
5
4
3
2
1
0
Cichorium intybus Lamium purpureum Viscum album
a
a
a
c
ab
a
b
b
b
Polyphenols Flavonoids Phenolic acids
Fig. 1. The total content of polyphenolic compounds, flavonoids and phenolic acids
in the aboveground part of investigated plants (means in columns followed by
different letters are different at p = 0.05)
91ISSN 16056574. Інтродукція рослин, 2019, № 3
Content of phenolic compounds in plant raw of Cichorium intybus L., Lamium purpureum L. and Viscum album L.
V. album that grew on Tilia cordata 10.34 and 11.32
mg GAE/g respectively [50]. In different extracts
of V. album, TPC was from 10.92 to 37.66 mg
GAE/g as reported Orhan et al. (2014) [38].
The total content of flavonoids (TFC) for C. in
ty bus, L. purpureum and V. album was 26.29, 28.89
and 25.10 mg QE/g respectively. According to Ab
bas et al. (2015), flavonoid content of extracts of
C. intybus was 6.82 mg/g of rutin equivalent [1].
Wild and cultivated chicory plants had TFC 2.29—
4.42 and 1.57—2.01 µM of caffeic acid equivalent
respectively [25]. GrujiN et al. (2017) found that
the concentration of flavonoids in ethanol extracts
of L. purpureum was 32.8 mg QE/g [20]. As de
scribed by Kang (2016), the content of total flavo
noids for V. album leaves extracts was 36.38 mg
QE/g [27]. TFC of V. album extracts was from
1.76 to 9.11 mg QE/g, as reported by Orhan et al.
(2014) [38].
The total content of phenolic acids (TPA) for
C. intybus, L. purpureum, and V. album was 4.56, 4.87
and 4.07 mg CAE/g respectively. As described in
TahiroviN and BaO iN (2017), TPA in stems and leaves
was 1.31 and 1.59 mg CAE/g respectively [50].
Antioxidant activity of extracts in this study de
termined by two methods: DPPHmethod and
phosphomolybdenum (Fig. 2).
Antioxidant activity (AA) of extracts by DPPH
method for C. intybus, L. purpureum and V. album
was 8.35, 7.66 and 8.55 mg Trolox Equivalent/g.
In some report, antioxidant activity by DPPHme
thod for C. intybus was 67.2 µg/ml [1].
AA by phosphomolybdenum method for C. inty
bus, L. purpureum and V. album was 93.01, 142.62
and 9.31 mg Trolox Equivalent per gram. GruiN
et al. (2017) indicated that reducing power of dif
ferent extracts of L. purpureum increased with in
creasing of concentration of extracts [20].
Conducted correlation analyze showed that found
a strong correlation between antioxidant activity
and polyphenol compounds of investigated plant
species. Study of C. intybus extracts identified strong
positive correlation between antioxidant activity
by DPPH method and content of polyphenols (r =
= 0.996), AA by DPPH method and flavonoids
(r = 0.967) and AA by DPPH method and phe
nolic acids (r = 0.971); strong positive correlation
between AA by PhMo and polyphenols (r = 0.971),
AA by PhMo and flavonoids (r = 0.997), AA by
PhMo and phenolic acids (r = 0.884). Study of
L. purpureum demonstrated strong positive correla
tion between antioxidant activity by DPPH meth
od and content of polyphenols (r = 0.977), AA
by DPPH method and flavonoids (r = 0.976) and
A
n
ti
ox
id
an
t
ac
ti
vi
ty
by
D
P
P
H
-m
et
h
od
,
m
g
T
E
/g
9
8
7
6
5
4
3
2
1
0
A
n
ti
ox
id
an
t
ac
ti
vi
ty
by
p
h
os
ph
om
ol
yb
de
n
um
m
et
h
od
,
m
g
T
E
/g
140
120
100
80
60
40
20
0
Cichorium intybus Lamium purpureum Viscum album
aaa
b b
b
DPPH-method Phospomolybdenum method
Fig. 2. Antioxidant activity of ethanol extracts of Cichorium intybus L., Lamium
purpureum L. and Viscum album L. (means in columns followed by different letters
are different at p = 0.05)
92 ISSN 16056574. Plant introduction, 2019, № 3
O.M. Vergun, O.V. Grygorieva, J. Brindza, O.V. Shymanska, D.B. Rakhmetov, V. HorP inovQ SedlaP kovQ , O.A. Korablova...
AA by DPPH method and phenolic acids (r =
= 0.929); strong positive correlation between AA
by PhMo and polyphenols (r = 0.882), AA by
PhMo and flavonoids (r = 0.881), AA by PhMo
and phenolic acids (r = 0.792). Between accumu
lations investigated compounds and AA of V. al
bum extracts found following relations: strong
positive correlation between antioxidant activity
by DPPH method and content of polyphenols
(r = 0.927) and AA by DPPH method and flavo
noids (r = 0.925); strong positive correlation be
tween AA by PhMo and polyphenols (r = 0.997)
and AA by PhMo and flavonoids (r = 0.998). Be
tween AA and phenolic acids, accumulation found
a negative correlation.
Conclusions
Thus, wild plants of Cichorium intybus, Lamium
purpureum and Viscum album in M.M. Gryshko
National Botanical Garden of the NAS of Ukraine
accumulated polyphenol compounds with high
antioxidant activity. Obtained data demonstrated
that these plant species can be a potential source
of natural antioxidants that can be used in the
pharmacological investigations. It is necessary to a
branch of biological science to investigate bio
chemical properties of not cultivated plants only
but wild plants also to identify new sources of bio
logically active compounds.
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logy, vol. 4, N 3, pp. 1—5.
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(2015), Pharmacological and molecular activity of me
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(2010), Antioxidant and antimutagenic activities of
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Recommended by E. Ma ahonb akovc
Received 17.05.2019
95ISSN 16056574. Інтродукція рослин, 2019, № 3
Content of phenolic compounds in plant raw of Cichorium intybus L., Lamium purpureum L. and Viscum album L.
О.М. Вергун 1, О.В. Григор’єва 1, Я. Бріндза 2, О.В. Шиманська 1,
Д.Б. Рахметов 1, В. Горчінова Седлачкова 2,
О.А. Корабльова 1, В.В. Фіщенко 1, Е. Іванішова 2
1 Національний ботанічний сад імені М.М. Гришка
НАН України, Україна, м. Київ
2 Словацький Аграрний університет у Нітрі,
Словакія, м. Нітра
ВМІСТ ФЕНОЛЬНИХ СПОЛУК У РОСЛИННІЙ
СИРОВИНІ CICHORIUM INTYBUS L., LAMIUM
PURPUREUM L. ТА VISCUM ALBUM L.
Мета — оцінити антиоксидантний потенціал ета
ноль них екстрактів відібраних в умовах Національного
бо танічного саду імені М.М. Гришка НАН України
дикорослих рослин шляхом визначення фенольних
сполук.
Матеріал та методи. Використовували суху сирови
ну Cychorium intybus L., Lamium purpureum L. та Viscum
album L. Рослини Cychorium intybus і Lamium purpu
reum зіб рано в Національному ботанічному саду імені
М.М. Гришка НАН України, рослини Viscum album — з
крони дерев Tilia cordata Mill. Протягом 2 год 0,2 г су
хої рослинної сировини екстрагували з 20 мл 80 %
етанолу. Загальний вміст поліфенолів в екстрактах
вимірювали методом, описаним Singleton і Rossi (1965)
з використанням реактиву Фоліна—Чокалтеу. Резуль
тати виражені у мг галової кислоти (еквівалент) на
грам сухої речовини (мг ГКЕ/г). Визначення загаль
ного вмісту флавоноїдів проведено за модифікованим
методом, описаним Shafii et al. (2017). Результати ви
ражено у мг кверцетинеквівалента на 1 г сухої речо
вини (КЕ/г). Визначення загального вмісту феноль
них кислот в екстрактах проведено методом, описа
ним у Farmakopea Polska (1999). Результати виражено
у мг кофейної кислоти (еквівалент) на 1 г сухої речо
вини (мг ККЕ/г). Антирадикальну активність зразків
вимірювали з 2,2дифеніл1пікрилгідразилом (ДФПГ)
за методом SanchezMoreno et al. (1998). Антиоксидант
ну активність визначали фосфомолібденовим мето
дом, описаним Р. Prieto et al. (1999) з незначною мо
дифікацією. Результати виражені у мг тро локсек ві
ва лента на 1 г сухої речовини (мг ТЕ/г). Експеримен
тальні дані опрацьовано в Excel 2010. Кореляційний
аналіз проведено з використанням критерію Пірсона.
Результати. Загальний вміст поліфенолів для рос
лин C. intybus, L. purpureum та V. album становив 33,91,
34,61 та 31,28 мг ГКЕ/г відповідно, флавоноїдів —
26,29, 28,89 та 25,10 мг КЕ/г, фенольних кислот —
4,56, 4,87 та 4,07 мг ККE/г відповідно, антиоксидантна
активність екстрактів, визначена методом ДФПГ, —
8,35, 7,66 та 8,55 мг ТЕ/г, антиоксидантна активність,
визначена фосфомолібденовим методом, — 93,01,
142,62 та 9,31 мг ТЕ/г. Установлено стійку прямо про
порційну кореляцію між накопиченням поліфеноль
них сполук і антиоксидантною активністю екстрактів.
Висновки. Дикорослі рослини C. intybus, L. purpu
reum та V. album у Національному ботанічному саду
імені M.M. Гришка НАН України накопичували по
ліфенольні сполуки з високою антиоксидантною ак
тивністю. Отримані дані свідчать, що ці види можуть
бути потенційним джерелом природних антиоксидан
тів, що можна використовувати у фармакологічних
дослідженнях. Визначення біохімічних особливостей
не лише культурних, а і дикорослих рослин є важли
вим напрямом сучасної біологічної науки для вияв
лення нових джерел біологічно активних речовин.
Ключові слова: Cichorium intybus, Lamium purpureum,
Viscum album, поліфеноли, флавоноїди, фенольні кис
лоти, антиоксидантна активність.
Е.Н. Вергун 1, О.В. Григорьева 1, Я. Бриндза 2,
О.В. Шиманская 1, Д.Б. Рахметов 1,
В. Горчинова Седлачкова 2, О.А. Кораблева 1,
В.В. Фищенко 1, Е. Іванишова 2
1 Национальный ботанический сад
имени Н.Н. Гришко НАН Украины,
Украина, г. Киев
2 Словацкий Аграрный университет в Нитре,
Словакия, г. Нитра
СОДЕРЖАНИЕ ФЕНОЛЬНЫХ СОЕДИНЕНИЙ
В РАСТИТЕЛЬНОМ СЫРЬЕ CICHORIUM
INTYBUS L., LAMIUM PURPUREUM L.
И VISCUM ALBUM L.
Цель — оценить антиоксидантный потенциал этаноль
ных экстрактов отобранных в условиях Националь
ного ботанического сада имени Н.Н. Гришко НАН
Украины дикорастущих растений путем определения
фенольних соединений.
Материалы и методы. Использовали сухое сырье
Cychorium intybus L., Lamium purpureum L. и Viscum al
bum L. Растения Cychorium intybus и Lamium purpureum
собраны в Национальном ботаническом саду имени
Н.Н. Гришко НАН України, растения Viscum album —
с кроны деревьев Tilia cordata Mill. На протяжении 2 ч
0,2 г сухого растительного сырья экстрагировали в 20 мл
80 % этанола. Общее содержание полифенолов в экс
трактах измеряли методом, описанным Singleton и
Rossi (1965) с использованием реактива Фолина—Чо
калтеу. Результаты виражены в мг галовой кислоты
(эквивалент) на 1 г сухого вещества (мг ГКЭ/г). Опре
деление общего содержания флавоноидов проводили
модифицированным методом, описанным Shafii et al.
(2017). Результаты выражены в мг кверцетинэкви ва
лента на 1 г сухого вещества (КЭ/г). Определение
общего содержания фенольных кислот экстрактов
проводили методом, описанным в Farmakopea Polska
(1999). Результаты выражены в мг кофейной кислоты
(эквивалент) на 1 г сухого вещества (мг ККЭ/г).
96 ISSN 16056574. Plant introduction, 2019, № 3
O.M. Vergun, O.V. Grygorieva, J. Brindza, O.V. Shymanska, D.B. Rakhmetov, V. Hord inove Sedlad kove , O.A. Korablova...
Антирадикальную активность образцов измеряли с
2,2дифенил1пикрилгидразилом (ДФПГ) по методу
SanchezMoreno et al. (1998). Антиоксидантную ак
тивность определяли фосфомолибденовым методом,
описанным Prieto et al. (1999) с незначительной мо
дификацией. Результаты выражены в мг тролокс
эквивалента на 1 г сухого вещества (мг ТЭ/г). Експе
риментальные данные обработаны в Excel 2010.
Кор реляционный анализ проводили с использовани
ем критерия Пирсона.
Результаты. Общее содержание полифенолов для
растений C. intybus, L. purpureum и V. album составило
33,91, 34,61 и 31,28 мг ГКЭ/г соответственно, флаво
ноидов — 26,29, 28,89 и 25,10 мг КЭ/г, фенольных
кислот — 4,56, 4,87 и 4,07 мг ККЭ/г, антиоксидант
ная активность экстрактов, определенная методом
ДФПГ, — 8,35, 7,66 и 8,55 мг ТЭ/г, антиоксидантная
активность, определенная фосфомолибденовым ме
тодом, — 93,01, 142,62 и 9,31 мг ТЭ/г. Установлена
стойкая прямо пропорциональная корреляция между
накоплением полифенольных соединений и антиок
сидантной активностью экстрактов.
Выводы. Дикорастущие растения C. intybus, L. pur
pu reum и V. album в Национальном ботаническом
саду имени Н.Н. Гришко НАН Украины накаплива
ли по лифенольные соединения с высокой антиок
сидантной активностью. Полученные данные сви де
тельст вуют, что данные виды растений могут быть
по тенциальным источником природных ан ти ок си
дан тов, что можно использовать в фармакологиче
ских исследованиях. Определение биохимических
осо бен ностей не только культурных, но и дикорасту
щих растений является важным направлением со
временной биологической науки для для выявления
новых источников биологически активных веществ.
Ключевые слова: Cichorium intybus, Lamium purpureum,
Viscum album, полифенолы, флавоноиды, фенольные
кислоты, антиоксидантная активность.
|
| id | oai:ojs2.plantintroduction.org:article-1520 |
| institution | Plant Introduction |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2025-07-17T12:53:21Z |
| publishDate | 2019 |
| publisher | M.M. Gryshko National Botanical Garden of the NAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | wwwplantintroductionorg/6e/8b8de58a24dbe2ef18a86b07e9df716e.pdf |
| spelling | oai:ojs2.plantintroduction.org:article-15202019-11-11T08:16:08Z Content of phenolic compounds in plant raw of Cichorium intubus L., Lamium purpureum L. and Viscum album L. Вміст фенольних сполук у рослинній сировині Cichorium intybus L., Lamium purpureum L. та Viscum album l. Vergun, O.M. Grygorieva, O.V. Brindza, J. Shymanska, O.V. Rakhmetov, D.B. Horčinová-Sedlačková, V. Korablova, O.A. Fishchenko, V.V. Ivanišová, E. Objective – to evaluate the antioxidant potential of ethanol extracts of wild selected plants in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine through the determination of phenolic compounds. Material and methods. In this study used dried raw of Cichorium intybus L., Lamium purpureum L. and Viscum album L. Plants of C. intybus and L. purpureum harvested from natural flora of the M.M. Gryshko National Botanical Garden of the NAS of Ukraine. V. album collected from crown trees of Tilia cordata Mill. 0.2 g of dried plant raw material was extracted with 20 mL of 80 % ethanol for 2 hours. The total polyphenol content of extracts was measured by the method described by Singleton and Rossi (1965) using Folin-Chiocalteu reagent and results were expressed in mg of gallic acid equivalent per one gram of dry matter (mg GAE/g). Determination of total flavonoids content was conducted using the modified method described in Shafii et al. (2017) and results expressed in mg quercetin equivalent per one gram of dry matter (mg QE/g). Detection of total phenolic acids content of extracts was carried out using the method described in Farmakopea Polska (1999) and results expressed in mg caffeic acid per one gram of dry matter (mg CAE/g). The antioxidant activity of samples was measured using 2.2-diphenyl-1-picrylhydrazyl (DPPH method) according to Sanchez-Moreno et al. (1998). Also, the antioxidant activity of extracts was determined by the phospho-molybdenum method described by Prieto et al. (1999) with slight modifications. Results of these parameters expressed in mg Trolox equivalent per one gram of dry matter (mg TE/g). Experimental data were evaluated by using Excel 2010. Correlation analysis performed using Pearson’s criterion. Results. The total content of polyphenol compounds for C. intybus, L. purpureum and V. album was 33.91, 34.61 and 31.28 mg GAE/g, respectively, the total content of flavonoids for C. intybus, L. purpureum and V. album – 26.29, 28.89 and 25.10 mg QE/g, the total content of phenolic acids – 4.56, 4.87 and 4.07 mg CAE/g, antioxidant activity of extracts by DPPH method was 8.35, 7.66 and 8.55 mg Trolox Equivalent/g, respectively, antioxidant activity by phosphomolybdenum method – 93.01, 142.62 and 9.31 mg Trolox Equivalent/g. Between the accumulation of polyphenol compounds and antioxidant activity of extracts found a strong positive correlation. Conclusions. Wild plants of C. intybus, L. purpureum and V. album in M.M. Gryshko National Botanical Garden of the NAS of Ukraine accumulated polyphenol compounds with high antioxidant activity. Obtained data demonstrated that these plant species can be a potential source of natural antioxidants that can be used in the different pharmacological investigations. It is important to a branch of biological science to investigate biochemical properties of not cultivated plants only but wild plants also to identify new sources of biologically active compounds. Мета – оцінити антиоксидантний потенціал етанольних екстрактів відібраних в умовах Національного ботанічного саду імені М.М. Гришка НАН України дикорослих рослин шляхом визначення фенольних сполук. Матеріал та методи. Використовували суху сировину Cychorium intybus L., Lamium purpureum L. та Viscum album L. Рослини Cychorium intybus і Lamium purpureum зібрано в Національному ботанічному саду імені М.М. Гришка НАН України, рослини Viscum album – з крони дерев Tilia cordata Mill. Протягом 2 год 0,2 г сухої рослинної сировини екстрагували з 20 мл 80 % етанолу. Загальний вміст поліфенолів в екстрактах вимірювали методом, описаним Singleton &amp; Rossi (1965) з використанням реактиву Фоліна–Чокалтеу. Результати виражені у мг галової кислоти (еквівалент) на грам сухої речовини (мг ГКЕ/г). Визначення загального вмісту флавоноїдів проведено за модифікованим методом, описаним Shafii et al. (2017). Результати виражено у мг кверцетин-еквівалента на 1 г сухої речовини (КЕ/г). Визначення загального вмісту фенольних кислот в екстрактах проведено методом, описаним у Farmakopea Polska (1999). Результати виражено у мг кофейної кислоти (еквівалент) на 1 г сухої речовини (мг ККЕ/г). Антирадикальну активність зразків вимірювали з 2,2-дифеніл-1-пікрилгідразилом (ДФПГ) за методом Sanchez-Moreno et al. (1998). Антиоксидантну активність визначали фосфомолібденовим методом, описаним Р. Prieto et al. (1999) з незначною модифікацією. Результати виражені у мг тролокс-еквівалента на 1 г сухої речовини (мг ТЕ/г). Експериментальні дані опрацьовано в Excel 2010. Кореляційний аналіз проведено з використанням критерію Пірсона. Результати. Загальний вміст поліфенолів для рослин C. intybus, L. purpureum та V. album становив 33,91, 34,61 та 31,28 мг ГКЕ/г відповідно, флавоноїдів – 26,29, 28,89 та 25,10 мг КЕ/г, фенольних кислот – 4,56, 4,87 та 4,07 мг ККE/г відповідно, антиоксидантна активність екстрактів, визначена методом ДФПГ, – 8,35, 7,66 та 8,55 мг ТЕ/г, антиоксидантна активність, визначена фосфомолібденовим методом, – 93,01, 142,62 та 9,31 мг ТЕ/г. Установлено стійку прямо пропорційну кореляцію між накопиченням поліфенольних сполук і антиоксидантною активністю екстрактів. Висновки. Дикорослі рослини C. intybus, L. purpureum та V. album у Національному ботанічному саду імені M.M. Гришка НАН України накопичували поліфенольні сполуки з високою антиоксидантною активністю. Отримані дані свідчать, що ці види можуть бути потенційним джерелом природних антиоксидантів, що можна використовувати у фармакологічних дослідженнях. Визначення біохімічних особливостей не лише культурних, а і дикорослих рослин є важливим напрямом сучасної біологічної науки для виявлення нових джерел біологічно активних речовин. M.M. Gryshko National Botanical Garden of the NAS of Ukraine 2019-09-01 Article Article application/pdf https://www.plantintroduction.org/index.php/pi/article/view/1520 10.5281/zenodo.3404149 Plant Introduction; Vol 83 (2019); 87-96 Інтродукція Рослин; Том 83 (2019); 87-96 2663-290X 1605-6574 10.5281/zenodo.3466025 en https://www.plantintroduction.org/index.php/pi/article/view/1520/1466 http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Vergun, O.M. Grygorieva, O.V. Brindza, J. Shymanska, O.V. Rakhmetov, D.B. Horčinová-Sedlačková, V. Korablova, O.A. Fishchenko, V.V. Ivanišová, E. Вміст фенольних сполук у рослинній сировині Cichorium intybus L., Lamium purpureum L. та Viscum album l. |
| title | Вміст фенольних сполук у рослинній сировині Cichorium intybus L., Lamium purpureum L. та Viscum album l. |
| title_alt | Content of phenolic compounds in plant raw of Cichorium intubus L., Lamium purpureum L. and Viscum album L. |
| title_full | Вміст фенольних сполук у рослинній сировині Cichorium intybus L., Lamium purpureum L. та Viscum album l. |
| title_fullStr | Вміст фенольних сполук у рослинній сировині Cichorium intybus L., Lamium purpureum L. та Viscum album l. |
| title_full_unstemmed | Вміст фенольних сполук у рослинній сировині Cichorium intybus L., Lamium purpureum L. та Viscum album l. |
| title_short | Вміст фенольних сполук у рослинній сировині Cichorium intybus L., Lamium purpureum L. та Viscum album l. |
| title_sort | вміст фенольних сполук у рослинній сировині cichorium intybus l., lamium purpureum l. та viscum album l. |
| url | https://www.plantintroduction.org/index.php/pi/article/view/1520 |
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