Екстракти листків деяких культурних рослин як потенційне джерело антиоксидантів
Objective — to evaluate the antioxidant potential of leaves extracts of four selected forage plants in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. In this study used dried raw of Brassica campestris L. f. annua DC., cv. Chanita (Brassicaceae Burn...
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| Дата: | 2019 |
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| Формат: | Стаття |
| Мова: | Англійська |
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M.M. Gryshko National Botanical Garden of the NAS of Ukraine
2019
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Репозитарії
Plant Introduction| _version_ | 1860145068044713984 |
|---|---|
| author | Vergun, O.M. Rakhmetov, D.B. Shymanska, O.V. Fishchenko, V.V. Ivanišová, E. Brindza, J. |
| author_facet | Vergun, O.M. Rakhmetov, D.B. Shymanska, O.V. Fishchenko, V.V. Ivanišová, E. Brindza, J. |
| author_sort | Vergun, O.M. |
| baseUrl_str | https://www.plantintroduction.org/index.php/pi/oai |
| collection | OJS |
| datestamp_date | 2019-12-14T17:56:03Z |
| description | Objective — to evaluate the antioxidant potential of leaves extracts of four selected forage plants in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine.
Material and methods. In this study used dried raw of Brassica campestris L. f. annua DC., cv. Chanita (Brassicaceae Burnett), Desmodium canadensis DC. (Fabaceae Lindl.), Rhaponticum carthamoides (Willd.) Iljin. (Asteraceae Bercht. & J. Presl.), Sinapis alba L. cv. Karolina (Brassicaceae Burnett) harvested from collections of the M.M. Gryshko National Botanical Garden of the NAS of Ukraine. 0.2 g of dried plant raw material was extracted with 20 mL of 80 % ethanol for 2 hours. The total polyphenol content of extracts was measured by the method described by Singleton and Rossi (1965) using Folin–Chiocalteu reagent and results were expressed in mg of gallic acid equivalent per one gram of dry matter (mg GAE/g). Determination of total flavonoids content was conducted using the modified method described in Shafii et al. (2017) and results expressed in mg quercetin equivalent per one gram of dry matter (mg QE/g). Detection of total phenolic acids content of extracts was carried out using the method described in Farmakopea Polska (1999) and results expressed in mg caffeic acid per one gram of dry matter (mg CAE/g). The antioxidant activity of samples was measured using 2.2-diphenyl-1-picrylhydrazyl (DPPH method) according to Sanchez-Moreno et al. (1998). Also, the antioxidant activity of extracts was determined by the phosphomolybdenum method described by Prieto et al. (1999) with slight modifications. Results of these parameters expressed in mg Trolox equivalent per one gram of dry matter (mg TE/g). Experimental data were evaluated by using Excel 2010.
Results. Total content of polyphenol compounds of investigated leaves extracts was from 32.43 to 73.58 mg GAE/g, total content of phenolic acids – from 4.87 to 9.15 mg CAE/g, total content of flavonoids – from 10.16 to 92.61 mg QE/g. Determination of antioxidant activity by DPPH method demonstrated results from 7.15 to 9.00 mg TE/g and by phosphomolybdenum method – from 77.87 to 190.64 mg TE/g.
Conclusions. Investigation of ethanol leaves extracts of four forage plants identified high content of polyphenol compounds and flavonoids what characterizes these plants as a valuable source of biologically active compounds. Obtained results can be of interest for deep pharmacological investigations. |
| doi_str_mv | 10.5281/zenodo.3566626 |
| first_indexed | 2025-07-17T12:53:29Z |
| format | Article |
| fulltext |
82 ISSN 16056574. Plant introduction, 2019, № 4: 82—88
https://doi.org/ 10.5281/zenodo.3566626
UDC 581.192
O.M. VERGUN 1, D.B. RAKHMETOV 1, O.V. SHYMANSKA 1,
V.V. FISHCHENKO 1, E. IVANI& OV' 2, J. BRINDZA 2
1 M.M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine
Ukraine, 01014 Kyiv, Timiryazevska str., 1
2 Slovak Agricultural University in Nitra
Slovak Republic, 94976 Nitra, Trieda Andreja Hlinku, 2
olenavergun8003@gmail.com
LEAVES EXTRACTS OF SELECTED CROPS
AS POTENTIAL SOURCE OF ANTIOXIDANTS
Objective — to evaluate the antioxidant potential of leaves extracts of four selected forage plants in conditions of M.M. Gryshko
National Botanical Garden of the NAS of Ukraine.
Material and methods. In this study used dried raw of Brassica campestris L. f. annua D.C., cv. Chanita (Brassicaceae Burnett),
Desmodium canadensis D.C. (Fabaceae Lindl.), Rhaponticum carthamoides (Willd.) Iljin. (Asteraceae Bercht. & J. Presl.), Sina
pis alba L., cv. Karolina (Brassicaceae Burnett) harvested from collections of the M.M. Gryshko National Botanical Garden of
the NAS of Ukraine. 0.2 g of dried plant raw material was extracted with 20 mL of 80 % ethanol for 2 hours. The total polyphe
nol content of extracts was measured by the method described by Singleton and Rossi (1965) using Folin—Chiocalteu reagent
and results were expressed in mg of gallic acid equivalent per one gram of dry matter (mg GAE/g). Determination of total flavo
noids content was conducted using the modified method described in Shafii et al. (2017) and results expressed in mg quercetin
equivalent per one gram of dry matter (mg QE/g). Detection of total phenolic acids content of extracts was carried out using the
method described in Farmakopea Polska (1999) and results expressed in mg caffeic acid per one gram of dry matter (mg CAE/g).
The antioxidant activity of samples was measured using 2.2diphenyl1picrylhydrazyl (DPPH method) according to Sanchez
Mo reno et al. (1998). Also, the antioxidant activity of extracts was determined by the phosphomolybdenum method described
by Prieto et al. (1999) with slight modifications. Results of these parameters expressed in mg Trolox equivalent per one gram of dry
matter (mg TE/g). Experimental data were evaluated by using Excel 2010.
Results. Total content of polyphenol compounds of investigated leaves extracts was from 32.43 to 73.58 mg GAE/g, total content
of phenolic acids — from 4.87 to 9.15 mg CAE/g, total content of flavonoids — from 10.16 to 92.61 mg QE/g. Determination
of antioxidant activity by DPPH method demonstrated results from 7.15 to 9.00 mg TE/g and by phosphomolybdenum method —
from 77.87 to 190.64 mg TE/g.
Conclusions. Investigation of ethanol leaves extracts of four forage plants identified high content of polyphenol compounds
and flavonoids what characterizes these plants as a valuable source of biologically active compounds. Obtained results can be
of interest for deep pharmacological investigations.
Key words: Brassica campestris, Desmodium canadensis, Rhaponticum carthamoides, Sinapis alba, polyphenols, flavonoids,
phe nolic acids, antioxidant activity.
© O.M. VERGUN, D.B. RAKHMETOV, O.V. SHYMANSKA,
V.V. FISHCHENKO, E. IVANI ( OV) , J. BRINDZA, 2019
Biological activity of plants caused by the presence
of biologically active compounds that related to
different groups. One of the most actual and de
manded directions in this area is identification of
antioxidant agents and antioxidant activity in plant
raw material. Plants are valuable source of natural
antioxidants due to content of different groups
of phenolic compounds [33]. The founding of
new na tural powerful but nontoxic compounds
from plants extracts have been searching [27].
Study of forage plants and their antioxidant activ
ity is of interest of last year’s investigations [34].
Among forage plants can be highlighted following
crops:
• Brassica campestris L. is representative of Bras
sicaceae Burnett family. It is the most widely known
group of useful plants that includes many of food
and forage crops. Numerous studies resulted in
the antioxidant potential of raw due to the content
83ISSN 16056574. Інтродукція рослин, 2019, № 4
Leaves extracts of selected crops as potential source of antioxidants
of polyphenol compounds and investigated their
antimicrobial activity [2; 11]. In addition, using
of Brassicaceae plants has reduced risk of cancer
illness [6; 12];
• Desmodium canadensis D.C. belongs to Faba
ceae Lindl. family. Plants of this species are a rich
source of phytochemicals such as phenolic com
pounds, saponins, triterpenic steroids, alkaloids,
complex biologically active compounds in the vol
atile oil. Plant raw of different Desmodium species
demonstrated antileishmanial, immuno mo du la
to ry, antioxidant, antiulcer, cardioprotec tive, an
tidiabetic, antiviral, anticonvulsant, hepatoprotec
tive activities [1; 3; 9; 10];
• Rhaponticum carthamoides (Willd.) Iljin. is
a perennial plant belonging to Asteraceae Bercht.
& J. Presl., roots and leaves of which accumulated
biologically active compounds that used in folk
and traditional medicine. Plant extracts demon
strated antioxidant activity and no mutagenic ef
fect [4; 25];
• Extracts of Sinapis alba L. (Brassicaceae) de
monstrated the high antiproliferative, antioxidant
and good antimicrobial activities. This plant con
tains the glucosinolates (sinalbin) that well ac
cepted by the food industry. Finish compounds
obtained during sanalbin conversion by endoge
nous myrosinase are responsible for mustard fla
vor [6; 23; 34].
Objective — to evaluate the antioxidant poten
tial of leaves extracts of selected forage plants in
conditions of M.M. Gryshko National Botanical
Garden of the NAS of Ukraine.
Material and methods
Plant materials
In this work used dried leaves raw of selected crops:
Brassica campestris L. f. annua D.C., cv. Cha nita,
Desmodium canadensis D.C., Rhaponticum cart
hamoides (Willd.) Iljin., Sinapis alba L. from col
lections of the M.M. Gryshko National Botanical
Garden of the NAS of Ukraine.
Chemicals
All chemicals were analytical grade and were
purchased from Reachem (Slovakia) and Sigma
Ald rich (USA).
Sample preparation
0.2 g of dried plant raw material was extracted with
20 ml of 80 % ethanol for 2 hours. After centrifu
gation at 4000 g (Rotofix 32 A, Hettich, Germany)
for 10 min, the supernatant was used for the next
measurements: antioxidant activity, polyphenols,
and flavonoids.
Total polyphenol content
Total polyphenol content (TPC) of extracts was
measured by the method of Singleton and Rossi
(1965) using Folin—Chiocalteu reagent [24].
0.1 ml of each sample extract was mixed with
0.1 ml of the Folin—Chiocalteu reagent, 1 ml of
20 % (w/v) sodium carbonate and 8.8 ml of dis
tilled water. After 30 min in darkness, the absorb
ance at 700 nm was measured using the spectro
photometer Jenway (6405 UV/Vis, England). Gal
lic acid (25—250 mg/L; R2=0.996) was used as
the standard and the results were expressed in
mg/g gallic acid equivalents.
Total flavonoid content
Determination of total flavonoids content (TFC)
was conducted using the modified method de
scribed in Shafii et al. (2017) [21]. 0.5 ml of sam
ple extract was mixed with 0.1 ml of 10 % (w/v)
ethanolic solution of aluminum chloride, 0.1 ml
of 1 M sodium acetate and 4.3 ml of distilled wa
ter. After 30 min in darkness the absorbance at
415 nm was measured using the spectrophotome
ter Jenway (6405 UV/Vis, England). Quercetin
(0.01—0.5 mg/L; R2=0.997) was used as the stand
ard and the results were expressed in mg/g querce
tin equivalents.
Total phenolic acid content
Determination total phenolic acids content (TPA)
of extracts was carried out using the method de
scribed in Farmakopea Polska (1999) [8]. 0.5 ml
of sample extract was mixed with 0.5 ml of 0.5 M
hydrochloric acid, 0.5 ml Arnova reagent, 0.5 ml
of 1 M sodium hydroxide (w/v) and 0.5 ml of dis
tilled water. Absorbance at 490 nm was measured us
ing the spectrophotometer Jenway (6405 UV/Vis,
England). Caffeic acid (1—200 mg/L, R2=0.999)
was used as a standard and the results were ex
pressed in mg/g caffeic acid equivalents.
84 ISSN 16056574. Plant introduction, 2019, № 4
O.M. Vergun, D.B. Rakhmetov, O.V. Shymanska, V.V. Fishchenko, E. Ivani* ov+ , J. Brindza
Antioxidant activity by DPPH method
The radical scavenging activity of samples was meas
ured using 2.2diphenyl1picrylhydrazyl (DPPH)
according to SanchezMoreno et al., 1998 [20].
The extracts (0.5 ml) were mixed with 3.6 ml of
radical solution (0.025 g of DPPH in 100 ml etha
nol). The absorbance of the sample extract was
determined using the spectrophotometer Jenway
(6405 UV/Vis, England) at 515 nm. Trolox (6hyd
roxy2,5,7,8tetramethylchroman2carboxylic
acid) (10—100 mg/L; R2=0.988) was used as the
standard and the results were expressed in mg/g
Trolox equivalents.
Antioxidant activity by phosphomolybdenum method
Antioxidant activity of extracts was determined by
the phosphomolybdenum method of Prieto et al.
(1999) with slight modifications [16]. The mixture of
sample (1 ml), monopotassium phosphate (2.8 ml,
0.1 M), sulfuric acid (6 ml, 1 M), ammonium
heptamolybdate (0.4 ml, 0.1 M) and distilled wa
ter (0.8 ml) incubated at 90 °C for 120 min, then
rapidly cooled and detected by monitoring ab
sorbance at 700 nm using the spectrophotometer
Jenway (6405 UV/Vis, England). Trolox (10—
1000 mg/L; R2=0.998) was used as the standard
and the results were expressed in mg/g Trolox
equivalents.
Experimental data were evaluated by using Excel
2010. Data were analyzed with ANOVA test and
differences between means were compared through
the TukeyKramer test (, = 0.05); significance le
vel at p < 0.05. Mean values of three replicates and
standard deviation are given in Tables 1, 2.
Results and discussions
Last time study of antioxidant potential of plant
raw due to the content of polyphenol compounds
is a very discussed branch of modern biology [13].
In M.M. Gryshko National Botanical Garden has
carried out complex investigations including the
biochemical study of different groups of plants,
among which forage, medicinal, energetic, aro
matic, wild, etc. [17; 18; 28—30; 32]. Our previ
ous data showed that plants from different families
characterized by different values of DPPH scav
Table 1. The total content of polyphenol compounds, phenolic acids, and flavonoids in leaves extracts of selected crops
Samples
Polyphenol compounds, mg
GAE/g (DW)
Phenolic acids, mg
CAE/g (DW)
Flavonoids, mg
QE/g (DW)
Brassica campestris, cv. Chanita 41.69 ± 0.62b 4.87 ± 0.27c 28.37 ± 1.10b
Desmodium canadensis 71.43 ± 1.50a 8.70 ± 0.07ab 61.05 ± 1.80a
Rhaponticum carthamoides 32.43 ± 0.28c 6.75 ± 0.50c 10.16 ± 0.19c
Sinapis alba 73.58 ± 5.83a 9.15 ± 0.19a 62.91 ± 0.44a
N o t e: GAE — gallic acid equivalent; CAE — caffeic acid equivalent; QE — quercetin equivalent; DW — dry weight;
means in columns followed by different letters are different at p = 0.05.
Table 2. Antioxidant activity of leaves extracts of selected crops
Samples
AA by phosphomolybdenum method, mg
TE/g (DW)
AA by DPPHmethod, mg TE/g (DW)
Brassica campestris, cv. Chanita 91.94 ± 3.12b 8.17 ± 0.06a
Desmodium canadensis 190.64 ± 4.03a 8.29 ± 0.16a
Rhaponticum carthamoides 77.87 ± 6.43c 9.00 ± 0.05a
Sinapis alba 148.43 ± 2.03ab 7.15 ± 0.10ab
N o t e: AA — antioxidant activity; TE — Trolox equivalent; DW — dry weight; means in columns followed by different
letters are different at p = 0.05.
85ISSN 16056574. Інтродукція рослин, 2019, № 4
Leaves extracts of selected crops as potential source of antioxidants
enging activity depending on raw (leaves, stems,
flowers, inflorescences, seeds, roots, etc.) [31].
Leaves, as well as flowers, had the most antioxi
dant capacity among other organs of plants as
showed some studies [22].
Phenolic compounds it’s a very large group of
phytochemicals that correlated often with anti
oxidant activity of plant extracts [7]. As demon
strated in Table 1, the content of polyphenol com
pounds was of 32.43—73.58 mg GAE/g. According
to Ayoola et al. (2018), the content of po ly phenols
in stems and leaves of Desmodium abscendens was
10 and 24 mg GAE/g, respectively [1]. Study of
another species D. gyrans showed that phenolic
compounds determined in the acetone, methanol
and water extracts whereas in petroleum ether,
toluene, chloroform, ethyl acetate extracts didn’t
determine it [33].
In this study, the highest content of polyphenol
compounds found for Sinapis alba ethanol ex
tracts. Investigations of Thangi et al. (2016) re
sulted that seed extracts of S. alba demonstrated
content of polyphenols of 8 mg GAE/g [26]. Con
tent of polyphenols in Rhaponticum carthamoides
extracts obtained by Miliauskas et al. (2004) was
2.4 times less (13.3 mg GAE/g) comparing with
our results. Content of flavonoids in this study was
10.16 mg Rutin equivalent/g [15].
Content of phenolic acids of investigated plants
determined from 4.87 to 9.15 mg CAE/g. Phe
nolic acids are widespread phenolic compounds
in plant organism that perform different impor
tant functions (as defense molecules, chemoat
tractants, concentrationdependent root growth
inhibition, a stimulator of indole acetic acid, al
ternative source of carbon for some diazotrophs,
etc.) depending on plant species [14].
Also, we found that the content of flavonoids
was from 28.37 to 62.91 mg QE/g among investi
gated leaves extracts.
We determined the antioxidant capacity of eth
anol leaves extracts by both phosphomolybdenum
and DPPH methods (Table 2). We found that
antioxidant activity by phosphomolybdenum
method was from 77.87 to 190.64 mg TE/g and by
DPPH method from — 7.15 to 9.00 mg TE/g of
DW.
Antioxidant activity determination by different
methods in extracts of Rh. carthamoides, as re
ported Biskup and Lojkowska (2009), using of
phosphomolybdenum method identified more ac
tivity than others but the most interesting were
chloroform extracts [4]. Also, the antioxidant ac
tivity of investigated extracts depended on plants
origin [5].
Thangi et al. (2016) found a positive correlation
between the content of phenolic compounds and
antioxidant activity in Sinapis alba extracts [26].
In addition, leaves of representatives of Brassica
ceae characterized not antioxidant potential only
but have anticancer activity also [19].
Conclusions
Summarizing obtained data, it should be noticed
that leaves extracts of forage plants Brassica cam
pestris L. f. annua D.C., cv. Chanita, Desmodium
canadensis D.C., Rhaponticum carthamoides (Wil
ld.) Iljin., Sinapis alba L. from the M.M. Gryshko
National Botanical Garden of the NAS of Ukraine
had high values of antioxidant activity by two dif
ferent methods. The highest content of phenolic
compounds had extracts of plants D. canadensis
and S. alba. Evidently, that leaves of investigated
plants can be a valuable source of antioxidants and
obtained data can be used in further pharmaco
logical investigations.
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Recommended by O. Grygorieva
Received 29.07.2019
О.М. Вергун 1, Д.Б. Рахметов 1, О.В. Шиманська 1,
В.В. Фіщенко 1, Е. Іванішова 2, Я. Бріндза 2
1 Національний ботанічний сад імені М.М. Гришка
НАН України, Україна, м. Київ
2 Словацький аграрний університет у Нітрі,
Словацька Республіка, м. Нітра
ЕКСТРАКТИ ЛИСТКІВ ДЕЯКИХ
КУЛЬТУРНИХ РОСЛИН ЯК ПОТЕНЦІЙНЕ
ДЖЕРЕЛО АНТИОКСИДАНТІВ
Мета — оцінити антиоксидантний потенціал лист
кових екстрактів чотирьох кормових рослин в умовах
Національного ботанічного саду імені М.М. Гришка
НАН України.
Матеріал та методи. Використовували суху рослин
ну сировину Brassica campestris L. f. annua D.C., cv. Cha
nita (Brassicaceae Burnett), Desmodium canadensis D.C.
(Fabaceae Lindl.), Rhaponticum carthamoides (Willd.) Iljin.
(Asteraceae Bercht. & J. Presl.), Sinapis alba L., cv. Karo
lina (Brassicaceae Burnett), зібрану в колекціях Націо
нального ботанічного саду імені М.М. Гришка НАН
України. 0,2 г сухої рослинної сировини екстрагували
в 20 мл 80 % етанолу протягом 2 год. Загальний вміст
поліфенолів в екстрактах визначали методом, описа
ним у Singleton та Rossi (1965), використовуючи реагент
Фоліна—Чокалтеу. Результати представлено в мілігра
мах на 1 г еквівалента галової кислоти (мг ГКЕ/г).
Визначення вмісту флавоноїдів проводили методом,
описаним у Shafii et al. (2017). Результати представле
но в міліграмах на 1 г квер це тинеквівалента сухої
маси (мг КЕ/г). Визначення загального вмісту фе
нольних кислот проводили за методом, описаним у
Farmakopea Polska (1999). Результати представлено у
міліграмах еквівалента кофей ної кислоти на 1 г сухої
маси (мг ККЕ /г). Антиоксидантну активність зразків
вимірювали з 2,2ди феніл1пікрилгідразилом (ДФПГ
метод) згідно із San chezMore no et al. (1998). Також ви
значали антиоксидантну активність екстрактів фос
фомолібденовим методом, описаним у Prieto et al. (1999)
з незначною модифікацією. Результати цих досліджень
представлено в міліграмах тролоксеквівален та на 1 г
сухої речовини (мг TЕ/г). Експериментальні дані опра
цьовано в Excel 2010.
Результати. Загальний вміст поліфенольних речо
вин досліджуваних листкових екстрактів становив від
32,43 до 73,58 мг ГКЕ/г, загальний вміст фенольних
кислот — від 4,87 до 9,15 мг ККЕ/г, флавоноїдів — від
10,16 до 92,61 мг КЕ/г. Антиоксидантна активність,
визначена ДФПГметодом, становила від 7,15 до
9,00 мг TE/г, а визначена фосфомолібденовим мето
дом — від 77,87 до 190,64 мг TE/г.
88 ISSN 16056574. Plant introduction, 2019, № 4
O.M. Vergun, D.B. Rakhmetov, O.V. Shymanska, V.V. Fishchenko, E. Ivani7 ov8 , J. Brindza
Висновки. Дослідження етанольних екстрактів лист
ків чотирьох кормових рослин показало високий
вміст поліфенольних речовин та флавоноїдів, що ха
рактеризує ці рослини як цінне джерело біологічно
активних речовин. Отримані результати можуть ста
новити інтерес для поглиблених фармакологічних
досліджень.
Ключові слова: Brassica campestris, Desmodium canaden
sis, Rhaponticum carthamoides, Sinapis alba, поліфеноли,
флавоноїди, фенольні кислоти, антиоксидантна ак
тивність.
Е.Н. Вергун 1, Д.Б. Рахметов 1, О.В. Шиманская 1,
В.В. Фищенко 1, Э. Иванишова 2, Я. Бриндза 2
1 Национальный ботанический сад
имени Н.Н. Гришко НАН Украины,
Украина, г. Киев
2 Словацкий аграрный университет в Нитре,
Словацкая Республика, г. Нитра
ЭКСТРАКТЫ ЛИСТЬЕВ
НЕКОТОРЫХ КУЛЬТУРНЫХ РАСТЕНИЙ
КАК ПОТЕНЦИАЛЬНИЙ ИСТОЧНИК
АНТИОКСИДАНТОВ
Цель — оценить антиоксидантный потенциал листовых
экстрактов четырех кормовых растений в условиях На
ционального ботанического сада имени Н.Н. Гришко
НАН Украины.
Материал и методы. Использовали сухое раститель
ное сырье Brassica campestris L. f. annua D.C., cv. Cha nita
(Brassicaceae Burnett), Desmodium canadensis D.C. (Faba
ceae Lindl.), Rhaponticum carthamoides (Willd.) Iljin. (As
teraceae Bercht. & J. Presl.), Sinapis alba L., cv. Karolina
(Brassicaceae Burnett), собранное в коллекциях На
ционального ботанического сада имени Н.Н. Гришко
НАН Украины. 0,2 г сухого растительного сырья экс
трагировали в 20 мл 80 % этанола в течение 2 ч. Общее
содержание полифенолов в экстрактах определяли
методом, описанным у Singleton и Rossi (1965), с реа
гентом Фолина—Чокалтеу. Результаты представлены
в миллиграммах на 1 г эквивалента галловой кислоты
(мг ГКЭ/г). Определение содержания флавоноидов
проводили методом, описанным у Shafii et al. (2017).
Результаты представлены в миллиграммах на 1 г квер
цетинэквивалента сухой массы (мг КЭ/г). Опреде
ление общего содержания фенольных кислот про
водили по методу, описанному в Farmakopea Polska
(1999). Результаты представлены в миллиграммах эк
вивалента кофейной кислоты на 1 г сухой массы (мг
ККЭ/г). Антиоксидантную активность образцов из
меряли с 2,2дифенил1пикрил гид разилом (ДФПГ
метод) по SanchezMoreno et al. (1998). Также опреде
ляли антиоксидантную активность экстрактов фос
фомолибденовым методом, описанным Prieto et al.
(1999) с незначительной модификацией. Результаты
этих исследований представлены в миллиграммах тро
локсэквивалента на 1 г сухого вещества (мг TЭ/г).
Экспериментальные даные обработаны в Excel 2010.
Результаты. Общее содержание полифенольных
веществ исследованных листовых экстрактов состав
ляло от 32,43 до 73,58 мг ГКЭ/г, общее содержание
фенольных кислот — от 4,87 до 9,15 мг ККЭ/г, общее
содержание флавоноидов — от 10,16 до 92,61 мг КЭ/г.
Антиоксидантная активность, определенная ДФПГ
методом, составляла от 7,15 до 9,00 мг TЭ/г, а опреде
ленная фосфомолибденовым методом — от 77,87 до
190,64 мг TЭ/г.
Выводы. Исследования этанольных экстрактов лис
тьев четырех кормовых растений показало высокое
содержание полифенольных веществ и флавоноидов,
что характеризует данные растения как ценный ис
точник биологически активных веществ. Полученные
результаты могут представлять интерес для углублен
ных фармакологических исследований.
Ключевые слова: Brassica campestris, Desmodium cana
densis, Rhaponticum carthamoides, Sinapis alba, поли фе
нолы, флавоноиды, фенольные кислоты, анти ок си
дант ная активность.
|
| id | oai:ojs2.plantintroduction.org:article-1533 |
| institution | Plant Introduction |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2025-07-17T12:53:29Z |
| publishDate | 2019 |
| publisher | M.M. Gryshko National Botanical Garden of the NAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | wwwplantintroductionorg/b7/b912f1408294e7f8ac0b0ed430d59fb7.pdf |
| spelling | oai:ojs2.plantintroduction.org:article-15332019-12-14T17:56:03Z Leaves extracts of selected crops as potential source of antioxidants Екстракти листків деяких культурних рослин як потенційне джерело антиоксидантів Vergun, O.M. Rakhmetov, D.B. Shymanska, O.V. Fishchenko, V.V. Ivanišová, E. Brindza, J. Objective — to evaluate the antioxidant potential of leaves extracts of four selected forage plants in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. In this study used dried raw of Brassica campestris L. f. annua DC., cv. Chanita (Brassicaceae Burnett), Desmodium canadensis DC. (Fabaceae Lindl.), Rhaponticum carthamoides (Willd.) Iljin. (Asteraceae Bercht. &amp; J. Presl.), Sinapis alba L. cv. Karolina (Brassicaceae Burnett) harvested from collections of the M.M. Gryshko National Botanical Garden of the NAS of Ukraine. 0.2 g of dried plant raw material was extracted with 20 mL of 80 % ethanol for 2 hours. The total polyphenol content of extracts was measured by the method described by Singleton and Rossi (1965) using Folin–Chiocalteu reagent and results were expressed in mg of gallic acid equivalent per one gram of dry matter (mg GAE/g). Determination of total flavonoids content was conducted using the modified method described in Shafii et al. (2017) and results expressed in mg quercetin equivalent per one gram of dry matter (mg QE/g). Detection of total phenolic acids content of extracts was carried out using the method described in Farmakopea Polska (1999) and results expressed in mg caffeic acid per one gram of dry matter (mg CAE/g). The antioxidant activity of samples was measured using 2.2-diphenyl-1-picrylhydrazyl (DPPH method) according to Sanchez-Moreno et al. (1998). Also, the antioxidant activity of extracts was determined by the phosphomolybdenum method described by Prieto et al. (1999) with slight modifications. Results of these parameters expressed in mg Trolox equivalent per one gram of dry matter (mg TE/g). Experimental data were evaluated by using Excel 2010. Results. Total content of polyphenol compounds of investigated leaves extracts was from 32.43 to 73.58 mg GAE/g, total content of phenolic acids – from 4.87 to 9.15 mg CAE/g, total content of flavonoids – from 10.16 to 92.61 mg QE/g. Determination of antioxidant activity by DPPH method demonstrated results from 7.15 to 9.00 mg TE/g and by phosphomolybdenum method – from 77.87 to 190.64 mg TE/g. Conclusions. Investigation of ethanol leaves extracts of four forage plants identified high content of polyphenol compounds and flavonoids what characterizes these plants as a valuable source of biologically active compounds. Obtained results can be of interest for deep pharmacological investigations. Мета — оцінити антиоксидантний потенціал листкових екстрактів чотирьох кормових рослин в умовах Національного ботанічного саду імені М.М. Гришка НАН України. Матеріал та методи. Використовували суху рослинну сировину Brassica campestris L. f. annua D.C., cv. Chanita (Brassicaceae Burnett), Desmodium canadensis D.C. (Fabaceae Lindl.), Rhaponticum carthamoides (Willd.) Iljin. (Asteraceae Bercht. &amp; J. Presl.), Sinapis alba L., cv. Karolina (Brassicaceae Burnett), зібрану в колекціях Національного ботанічного саду імені М.М. Гришка НАН України. 0,2 г сухої рослинної сировини екстрагували в 20 мл 80 % етанолу протягом 2 год. Загальний вміст поліфенолів в екстрактах визначали методом, описаним у Singleton та Rossi (1965), використовуючи реагент Фоліна–Чокалтеу. Результати представлено в міліграмах на 1 г еквівалента галової кислоти (мг ГКЕ/г). Визначення вмісту флавоноїдів проводили методом, описаним у Shafii et al. (2017). Результати представлено в міліграмах на 1 г кверцетин-еквівалента сухої маси (мг КЕ/г). Визначення загального вмісту фенольних кислот проводили за методом, описаним у Farmakopea Polska (1999). Результати представлено у міліграмах еквівалента кофейної кислоти на 1 г сухої маси (мг ККЕ /г). Антиоксидантну активність зразків вимірювали з 2,2-дифеніл-1-пікрилгідразилом (ДФПГ-метод) згідно із Sanchez-Moreno et al. (1998). Також визначали антиоксидантну активність екстрактів фосфомолібденовим методом, описаним у Prieto et al. (1999) з незначною модифікацією. Результати цих досліджень представлено в міліграмах тролокс-еквівален та на 1 г сухої речовини (мг TЕ/г). Експериментальні дані опрацьовано в Excel 2010. Результати. Загальний вміст поліфенольних речовин досліджуваних листкових екстрактів становив від 32,43 до 73,58 мг ГКЕ/г, загальний вміст фенольних кислот – від 4,87 до 9,15 мг ККЕ/г, флавоноїдів – від 10,16 до 92,61 мг КЕ/г. Антиоксидантна активність, визначена ДФПГ-методом, становила від 7,15 до 9,00 мг TE/г, а визначена фосфомолібденовим методом – від 77,87 до 190,64 мг TE/г. Висновки. Дослідження етанольних екстрактів листків чотирьох кормових рослин показало високий вміст поліфенольних речовин та флавоноїдів, що характеризує ці рослини як цінне джерело біологічно активних речовин. Отримані результати можуть становити інтерес для поглиблених фармакологічних досліджень. M.M. Gryshko National Botanical Garden of the NAS of Ukraine 2019-12-01 Article Article application/pdf https://www.plantintroduction.org/index.php/pi/article/view/1533 10.5281/zenodo.3566626 Plant Introduction; Vol 84 (2019); 82-88 Інтродукція Рослин; Том 84 (2019); 82-88 2663-290X 1605-6574 10.5281/zenodo.3572674 en https://www.plantintroduction.org/index.php/pi/article/view/1533/1476 http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Vergun, O.M. Rakhmetov, D.B. Shymanska, O.V. Fishchenko, V.V. Ivanišová, E. Brindza, J. Екстракти листків деяких культурних рослин як потенційне джерело антиоксидантів |
| title | Екстракти листків деяких культурних рослин як потенційне джерело антиоксидантів |
| title_alt | Leaves extracts of selected crops as potential source of antioxidants |
| title_full | Екстракти листків деяких культурних рослин як потенційне джерело антиоксидантів |
| title_fullStr | Екстракти листків деяких культурних рослин як потенційне джерело антиоксидантів |
| title_full_unstemmed | Екстракти листків деяких культурних рослин як потенційне джерело антиоксидантів |
| title_short | Екстракти листків деяких культурних рослин як потенційне джерело антиоксидантів |
| title_sort | екстракти листків деяких культурних рослин як потенційне джерело антиоксидантів |
| url | https://www.plantintroduction.org/index.php/pi/article/view/1533 |
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