Накопичення золи та фотосинтетичних пігментів у рослинній сировині видів роду Astragalus
Five Astragalus species (A. cicer, A. falcatus, A. galegiformis, A. glycyphyllos, and A. ponticus) grown at the M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine were investigated. Plant raw material was collected at differen...
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M.M. Gryshko National Botanical Garden of the NAS of Ukraine
2020
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| author | Bondarchuk, Oleksandr Vergun, Olena Shymanska, Oksana Fishchenko, Valentyna Rakhmetov, Dzhamal |
| author_facet | Bondarchuk, Oleksandr Vergun, Olena Shymanska, Oksana Fishchenko, Valentyna Rakhmetov, Dzhamal |
| author_sort | Bondarchuk, Oleksandr |
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| datestamp_date | 2023-08-26T20:39:33Z |
| description | Five Astragalus species (A. cicer, A. falcatus, A. galegiformis, A. glycyphyllos, and A. ponticus) grown at the M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine were investigated. Plant raw material was collected at different ontogenetic stages, including budding, flowering, and fruiting. After that, the content of ash, calcium, phosphorus, nitrogen, and photosynthetic pigments was analyzed and statistically processed.The content of ash varied from 3.12 to 5.22 %, calcium – from 0.31 to 0.91 %, phosphorus – from 0.07 to 0.14 %, and nitrogen – from 1.93 to 2.48 % depending on the stage of growth. The content of chlorophyll a varied from 0.901 to 2.475 mg/g FW (fresh weight), chlorophyll b – from 0.190 to 0.676 mg/g FW, and carotenoids – from 0.496 to 1.129 mg/g FW.Thus, the accumulation of ash and its components in the raw material of the investigated species depended on the period of growth and certain species. Content of photosynthetic pigments correlated with the content of phosphorus (r = 0.887–0.999) and with plant increment (r = 0.756–0.998) for all investigated species. Obtained data can be applied to analyze productivity and during the cultivation of these plants. |
| doi_str_mv | 10.46341/PI2020031 |
| first_indexed | 2025-07-17T12:53:44Z |
| format | Article |
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© The Authors. This content is provided under CC BY 4.0 license.
Plant Introduction, 87/88, 76–86 (2020)
RESEARCH ARTICLE
Accumulation of ash and photosynthetic pigments in the raw material of
Astragalus
Introduction
The genus Astragalus L. (Fabaceae Lindl.) is
one of the largest genera of vascular plants
with approximately 2500–3000 species.
It is distributed mainly in arid and semiarid
mountainous regions of the northern
hemisphere and South America (Ilçim &
Behçet, 2016). Certain Astragalus species
are used in folk medicine as cardiovascular,
antihypertensive, diuretic, choleretic remedies
(Lysiuk & Darmohray, 2016). Among the most
used parts of the plants are gum and roots
(Amiri et al., 2020).
Different biological effects and active
compounds were recently investigated for
some Astragalus species (Wang et al., 2019;
Zheng et al., 2020). Adigüzel et al. (2009)
found that methanol and hexane extracts
did not express neither antimicrobial nor
antifungal effect. However, soon Alrumman
et al. (2012) reported the antimicrobial activity
of A. atropilosulus (Hochst.) Bunge subsp.
abyssinicus (Hochst.) Gillett against Candida
spp., Drechslera halides, Fusarium oxysporum,
and Pythium ultimum. Some Astragalus
species also demonstrate antiinflammatory,
analgesic (Shojaii et al., 2013), anticancer
Oleksandr Bondarchuk *, Olena Vergun, Oksana Shymanska, Valentyna Fishchenko,
Dzhamal Rakhmetov
M.M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine, Tymiryazevska str. 1, 01014 Kyiv,
Ukraine;* bondbiolog@gmail.com
Received: 08.10.2020 | Accepted: 20.12.2020 | Published: 30.12.2020
Abstract
Five Astragalus species (A. cicer, A. falcatus, A. galegiformis, A. glycyphyllos, and A. ponticus) grown at the
M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine were investigated.
Plant raw material was collected at different ontogenetic stages, including budding, flowering, and fruiting.
After that, the content of ash, calcium, phosphorus, nitrogen, and photosynthetic pigments was analyzed
and statistically processed.
The content of ash varied from 3.12 to 5.22 %, calcium – from 0.31 to 0.91 %, phosphorus – from 0.07 to
0.14 %, and nitrogen – from 1.93 to 2.48 % depending on the stage of growth. The content of chlorophyll a
varied from 0.901 to 2.475 mg/g FW (fresh weight), chlorophyll b – from 0.190 to 0.676 mg/g FW, and
carotenoids – from 0.496 to 1.129 mg/g FW.
Thus, the accumulation of ash and its components in the raw material of the investigated species
depended on the period of growth and certain species. Content of photosynthetic pigments correlated
with the content of phosphorus (r = 0.887–0.999) and with plant increment (r = 0.756–0.998) for all
investigated species. Obtained data can be applied to analyze productivity and during the cultivation of
these plants.
Keywords: Astragalus, raw material, ash content, photosynthetic pigments, correlation
https://doi.org/10.46341/PI2020031
UDC 582.736.3: 581.192
https://creativecommons.org/licenses/by/4.0/
https://orcid.org/0000-0001-6367-9063
https://orcid.org/0000-0003-2924-1580
https://orcid.org/0000-0001-8482-5883
https://orcid.org/0000-0002-7714-1739
https://orcid.org/0000-0001-7260-3263
Plant Introduction • 87/88 77
Accumulation of ash and photosynthetic pigments in the raw material of Astragalus
(Auyeung et al., 2016), antioxidant (Jaradat
et al., 2017; Chasemian-Yadegari et al., 2019),
and antidiabetic (Guo et al., 2020) effects.
Finally, it was recently reported that flavonoids
from different Astragalus species have high
antioxidant activity (Berezutskii et al., 2020).
In addition, Astragalus species
possess tonic, hepatoprotective, diuretic,
antihyperglycemic features (Fu et al., 2014). The
strong pharmacological effect and potential
immunomodulatory effect of these species
appear due to biologically active compounds
such as saponins, flavonoids, polysaccharides,
phenolic acids, tannins, etc. Astragalus
contains 20 trace elements, 20 types of amino
acids, and fatty acids (Keskin, & Kacar, 2013;
Zhang, 2013; Fu et al., 2014; Sarraj-Laabidi et
al., 2018; Babich et al., 2019; Guo et al., 2019; Li
et al., 2019b).
Being the representatives of legume crops,
Astragalus plants are an excellent crude
protein source, low in fiber. Hence, these
plants can compete with other better-known
legumes, such as Medicago sativa L. (Asharya
et al., 2006).
Many years of investigations at the
M.M. Gryshko National Botanical Garden
of the National Academy of Sciences of
Ukraine (NBG) showed that Astragalus has
potential as forage, medicinal, bioenergetics,
and ornamental plants. These plants are
characterized by high productivity, rich
biochemical content, ecological tolerance,
and other favorable properties (Bondarchuk, &
Rakhmetov, 2016, 2017; Rakhmetov et al., 2018).
This study aimed to identify peculiarities of
certain biochemical parameters of Astragalus
species and find the relationship between
these parameters during the vegetation
period, as well as to evaluate Astragalus
species as perspective crops for the Forest-
Steppe of Ukraine.
Material and methods
Investigations were conducted in 2012–2018.
Plants from the experimental collection of the
biochemical laboratory of the Department of
the Cultural Flora of the NBG were used. Five
Astragalus species (A. cicer L., A. falcatus Lam.,
A. galegiformis L., A. glycyphyllos L., and
A. ponticus Pall.) were investigated during the
budding, flowering, and fruiting ontogenetic
stages.
The content of ash was determined
using the method of combustion in the
muffle-oven (SNOL 7,2/1100, Termolab) at
250–500 °C, according to Hrytsajenko et al.
(2003). After the combustion procedure,
obtained ash was used to determine the
content of calcium and phosphorus. The
calcium content was determined by acid
extracts’ titration from obtained ash
with Trilon B and given as calcium oxide
(Kalnytskyi, 1997).
Figure 1. Сontent of ash and some macroelements in the raw material of Astragalus species: 1 – A. cicer,
2 – A. falcatus, 3 – A. galegiformis, 4 – A. glycyphyllos, 5 – A. ponticus.
78 Plant Introduction • 87/88
O. Bondarchuk, O. Vergun, O. Shymanska, V. Fishchenko, D. Rakhmetov
The phosphorus content in plants was
determined in acid extracts from obtained
ash with molybdenum solution, and given as
phosphorus oxide (V) (Kalnytskyi, 1997).
The content of nitrogen was determined
by the chloramine method (Kalnytskyi, 1997)
after the crude ashing procedure (Yermakov
et al., 1972).
Photosynthetic pigments were determined
spectrophotometrically in the leaves following
Musijenko et al. (2001). Briefly, 100 mg of
fresh raw material was extracted with a fixed
volume of 80 % acetone in the presence of
magnesium carbonate. After filtration, the
supernatant was used to measure chlorophyll
a (Chl a), b (Chl b), and carotenoids content,
which was determined at the wavelengths of
662, 644, and 440 nm, respectively. Results
were calculated by the Lichtenthaler equation
and given in the mg/g of fresh weight (FW).
Also, obtained data were used to calculate the
ratio between photosynthetic pigments.
Three replicates were applied in each
treatment. The statistical processing of
experimental data was done using the main
descriptors: the arithmetic mean, standard
deviation, and correlation coefficient.
Correlation analysis was conducted using
Pearson’s criterion.
Results and discussion
In the previous study, we identified that sugars,
protein, ascorbic acid, and cellulose content
in five Astragalus species was the highest
at the flowering stage (Rakhmetov et al.,
2018). The current investigation represent
other biochemical parameters determined in
Astragalus raw material during the vegetation
period, i.e., the content of ash, calcium,
phosphorus, nitrogen, and photosynthetic
pigments.
The study of ash content in the plants is
an important parameter for evaluating the
raw material’s nutritive quality (Godočiková
et al., 2019). The assessment of this parameter
also applies to the investigation of energetic
crops, wherein found correlation between the
content of ash and heating value (Prochnow
et al., 2009). In studied Astragalus species,
the content of ash varied from 3.12 to 5.22 %,
depending on species and stage of growth
(Fig. 1). The content of calcium, phosphorus,
and nitrogen was 0.31–0.91 %, 0.07–0.14 %,
and 1.93–2.48 %, respectively. The content of
phosphorus decreased during vegetation in
raw material of all species.
Kumleh et al. (2016) reported that the
content of ash in the seed of A. podolobus
Boiss. & Hohen. was 10.42 %, which is two-
three times higher than our results for raw
material from the above-ground parts. A study
of another legume, Galega L., showed that ash,
phosphorus, and calcium in the raw material
is accumulated unevenly, and the content of
phosphorus is maximal at the beginning of
vegetation (Vergun et al., 2012). In particular,
stems of such energetic plants as Miscanthus
Andersson had an ash content of 1.38–1.60 %,
which is a few times less than those reported
by Vergun et al. (2019).
The content of photosynthetic pigments
is an essential parameter of plant growth
and an indicator of general photosynthetic
activity (Lichtenthaler, 1987). It is important
to combine investigations of photosynthetic
pigments during vegetation with analysis
of ecological factors and parameters of
growth (Zlatev & Lidon, 2012). The content
of chlorophylls changes with the change of
the environmental characteristics, and it is a
significant diagnostic indicator of plant growth
(Li et al., 2019a).
In this study, we investigated the
accumulation of photosynthetic pigments
in the leaves of Astragalus species during
vegetation. The content of chlorophyll a at the
budding stage varied from 2.117 to 2.475 mg/g
FW, chlorophyll b – from 0.445 to 0.676 mg/g
FW, and carotenoids – from 0.995 to 1.129
mg/g FW, depending on species (Fig. 2).
At the flowering stage, the content of
chlorophyll a varied from 1.329 to 2.111 mg/g
FW, chlorophyll b – from 0.354 to 0.567 mg/g
FW, and carotenoids – from 0.694 to 1.003
mg/g FW (Fig. 3).
At the fruiting stage, chlorophyll a was
accumulated in the leaves in the range from
0.901 to 1.913 mg/g FW, chlorophyll b – in
the range from 0.190 to 0.523 mg/g FW, and
carotenoids – from 0.496 to 0.912 mg/g FW
(Fig. 4).
Stress influences regulatory processes in
plants and photosynthetic ability that can
be noticed concerning the ratio between
carotenoids and the sum of chlorophylls.
In this case, the ratio Car / (Chl a + Chl b)
Plant Introduction • 87/88 79
Accumulation of ash and photosynthetic pigments in the raw material of Astragalus
Figure 2. Content of photosynthetic pigments in the leaves of Astragalus species at the budding stage.
Figure 4. Content of photosynthetic pigments in the leaves of Astragalus species at the fruiting stage.
Figure 3. Content of photosynthetic pigments in the leaves of Astragalus species at the flowering stage.
80 Plant Introduction • 87/88
O. Bondarchuk, O. Vergun, O. Shymanska, V. Fishchenko, D. Rakhmetov
is increasing (Filella et al., 2009). Gogoi &
Basumatary (2018) reported that ratio Chl a /
Chl b indicates the photosynthetic apparatus’s
development state and correlates with growth
parameters. According to Anderson (1986), the
regular ratios Chl a / Chl b for shade and light
plants are 1.6–2.2 and 2.6–3.4, respectively.
The ratio Chl a / Chl b in the investigated
Astragalus species at the budding stage was
from 3.46 to 4.82, the ratio of the sum of
chlorophylls to carotenoids – from 2.51 to 2.96,
and the ration of carotenoids to the sum of
chlorophylls – from 0.34 to 0.40 (Fig. 5).
At the flowering stage, the sum of
chlorophylls decreased comparing with
budding stage (Fig. 6). The ratio Chl a / Chl b
was 3.72–4.43, between the sum of
chlorophylls and carotenoids – 1.92–3.08, and
between carotenoids and sum of chlorophylls
– 0.32–0.52.
At the fruiting stage, the sum of chlorophylls
was the lowest and varied from 1.039 to 2.436
(Fig. 7). The ratio of Chl a / Chl b was also
lower and varied from 3.66 to 6.53. The ratio of
the sum of chlorophylls to carotenoids varied
from 2.06 to 2.67, and the ratio of carotenoids
to the sum of chlorophylls – from 0.31 to 0.48.
The increment during budding, flowering
and fruiting stage for A. cicer was 64.5 cm
(84.14 %), 21.5 cm (21.98 %), and 1.2 cm (1.2 %),
respectively. For A. falcatus the increment was
64.4 cm (28.12 %), 22.5 cm (21.69 %), and 6.3 cm
(5.73 %), respectively. For A. galegiformis it was
145.1 cm (91.02 %), 20.7 cm (11.49 %), and 12.6 cm
(6.54 %), respectively. For A. glycyphyllos it was
85.2 cm (87.21 %), 8.6 cm (8.09 %), and 3.5 cm
(3.18 %), respectively. And for A. ponticus it was
77.1 cm (81.85 %), 9.2 cm (8.89 %) and 3.3 cm
(3.09 %), respectively.
A very strong correlation between
chlorophylls and carotenoid content in A. cicer
has been identified (Table 1). Also, the content
of phosphorus, ash, and increment of plants
strongly correlated with photosynthetic
pigments’ content. Accumulation of calcium
and phosphorus showed a negative correlation
with pigments.
A similar strong correlation was also
identified between pigments’ content,
accumulation of phosphorus, and increment
of plants of A. falcatus (Table 2). A strong
correlation was also found between calcium
content and the ratio Chl a / Chl b. The same
relation was determined between ash content
and Car / (Chl a + Chl b). A negative correlation
was found between calcium content and the
accumulation of pigments.
A strong correlation was found between the
content of pigments, phosphorus, nitrogen,
ash, and increment of A. galegiformis (Table 3).
Similar strong correlation was also found
between (Chl a + Chl b) / Car and content of
phosphorus and nitrogen.
Like in a previous species, a strong
correlation was found between photosynthetic
pigments, phosphorus content, and increment
of A. glycyphyllos (Table 4).
For A. ponticus it was found a strong
correlation between phosphorus content and
pigments, increment, and (Chl a + Chl b) / Car
ratio (Table 5). Ratio Car / (Chl a + Chl b)
correlated with nitrogen and ash. Calcium,
nitrogen, and ash content demonstrated a
negative correlation with pigments.
Conclusions
Thus, the accumulation of ash in the raw
material of Astragalus species at the conditions
of the NBG varied and depends on period of
growth and certain species. Generally, this
parameter for all investigated species was in
the range of 3.12–5.22 %. The highest content
of calcium has been noticed at the fruitage for
all five species, whereas phosphorus content
was maximal at the budding stage for four
species excepting A. ponticus. Accumulation
of nitrogen was different, and its maximal
content for A. cicer, A. falcatus, and A. ponticus
was fixed at the fruiting stage. Accumulation
of photosynthetic pigments correlated with
phosphorus content (a very strong correlation,
r = 0.887–0.999) and increment (strong and
very strong correlations, r = 0.756–0.998) for
all investigated species. Also, a very strong
correlation was found between phosphorus
content and chlorophylls ratio (Chl a / Chl b).
Considering the limited number of reviews
about Astragalus photosynthetic content,
obtained data could be beneficial, especially
for studying the productivity of these plants.
Obtained data demonstrated peculiarities
of accumulation of ash and some of its
components during vegetation that can be
also useful at the cultivation of these plants in
the Forest-Steppe of Ukraine.
Plant Introduction • 87/88 81
Accumulation of ash and photosynthetic pigments in the raw material of Astragalus
Figure 5. Relationship between photosynthetic pigments content at the budding stage.
Figure 6. Relationship between photosynthetic pigments content at the flowering stage.
Figure 7. Relationship between photosynthetic pigments content at the fruiting stage.
82 Plant Introduction • 87/88
O. Bondarchuk, O. Vergun, O. Shymanska, V. Fishchenko, D. Rakhmetov
Parameter Chl a Chl b Car Chl a /
Chl b
Chl (a+b) /
Car
Car /
Chl (a+b) Ca P N Ash
Chl b 0.999 1
Car 0.982 0.983 1
Chl a / Chl b 0.977 0.976 0.920 1
Chl (a+b) / Car 0.702 0.701 0.557 0.838 1
Car / Chl (a+b) 0.785 0.785 0.656 0.900 0.992 1
Ca -0.913 -0.913 -0.821 -0.979 -0.932 -0.970 1
P 0.998 0.998 0.991 0.963 0.661 0.750 -0.889 1
N -0.945 -0.944 -0.867 -0.993 -0.897 -0.945 0.996 -0.925 1
Ash 0.979 0.979 0.924 0.999 0.833 0.895 -0.977 0.966 -0.992 1
Increment 0.998 0.998 0.992 0.962 0.658 0.747 -0.887 0.999 -0.923 0.965
Parameter Chl a Chl b Car Chl a /
Chl b
Chl (a+b) /
Car
Car /
Chl (a+b) Ca P N Ash
Chl b 0.998 1
Car 0.934 0.909 1
Chl a / Chl b -0.917 -0.940 -0.714 1
Chl (a+b) / Car 0.798 0.834 0.529 -0.972 1
Car / Chl (a+b) -0.793 -0.830 -0.523 0.970 -0.999 1
Ca -0.997 -0.991 -0.956 0.887 -0.753 0.749 1
P 0.995 0.999 0.892 -0.953 0.855 -0.852 -0.985 1
N 0.963 0.978 0.803 -0.990 0.931 -0.928 -0.942 0.986 1
Ash 0.999 0.997 0.940 -0.910 0.786 -0.782 -0.998 0.992 0.958 1
Increment 0.899 0.870 0.996 -0.651 0.453 -0.447 -0.928 0.849 0.748 0.907
Parameter Chl a Chl b Car Chl a /
Chl b
Chl (a+b) /
Car
Car /
Chl (a+b) Ca P N Ash
Chl b 0.929 1
Car 0.914 0.999 1
Chl a / Chl b -0.524 -0.801 -0.825 1
Chl (a+b) / Car 0.803 0.527 0.492 0.087 1
Car / Chl (a+b) -0.773 -0.484 -0.448 -0.136 -0.999 1
Ca -0.840 -0.981 -0.988 0.902 -0.352 0.304 1
P 0.998 0.999 0.991 0.963 0.661 0.750 -0.889 1
N -0.991 -0.971 -0.960 0.634 -0.716 0.679 0.905 -0.953 1
Ash -0.915 -0.702 -0.673 0.136 -0.975 0.963 0.550 -0.655 0.853 1
Increment 0.997 0.896 0.878 -0.453 0.849 -0.821 -0.793 0.866 -0.977 -0.945
Table 2. Correlation of investigated parameters of Astragalus falcatus.
Table 1. Correlation of investigated parameters of Astragalus cicer.
Table 3. Correlation of investigated parameters of Astragalus galegiformis.
Plant Introduction • 87/88 83
Accumulation of ash and photosynthetic pigments in the raw material of Astragalus
References
Adigüzel, A., Sökmen, M., Özkan, H., Ağar, G.,
Güllüce, M., & Şahin, F. (2009). In vitro
antimicrobial and antioxidant activities of
methanol and hexane extract of Astragalus
species growing in the Eastern Anatolia region
of Turkey. Turkish journal of Biology, 33, 65–71.
https://doi.org/10.3906/biy-0805-1
Alrumman, S. A., Moustafa, M. F. M., & Alamri, S. A.
(2012). Antibacterial and anti-fungal investigation
of Astragalus atropilosulus subsp. abyssinicus. African
Journal of Microbiology Research, 6(34), 6365–6369.
Amiri, M. S., Joharchi, M. R., Nadaf, M., & Nasseh, Y.
(2020). Ethnobotanical knowledge of Astragalus
spp.: The world’s largest genus of vascular plants.
Avicenna Journal of Phytomedicine, 10(2), 128–142.
Anderson, J. M. (1986). Photoregulation of the
composition function and structure of thylakoid
membranes. Annual Review of Plant Physiology,
37, 93–136. https://doi.org/10.1146/annurev.
pp.37.060186.000521
Asharya, S. N., Kastelic, J. P., Beauchemin,
K. A., & Messenger, D. F. (2006). A review
of research progress on cicer milkvetch
(Astragalus cicer L.). Canadian Journal of Plant
Science, 86, 49–62. https://doi.org/10.4141/
P04-174
Auyeung, K. K., Han, Q.-B., & Ko, K. J. (2016).
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protection against inflammation and
gastrointestinal cancers. The American Journal
of Chinese Medicine, 44(1), 1–22. https://doi.
org/10.1142/S0192415X16500014
Parameter Chl a Chl b Car Chl a /
Chl b
Chl (a+b) /
Car
Car /
Chl (a+b) Ca P N Ash
Chl b 0.997 1
Car 0.997 0.989 1
Chl a / Chl b -0.672 -0.723 -0.614 1
Chl (a+b) / Car 0.937 0.960 0.909 -0.887 1
Car / Chl (a+b) -0.956 -0.971 -0.996 0.537 -0.866 1
Ca -0.976 -0.989 -0.956 0.818 -0.991 0.925 1
P 0.989 0.997 0.976 -0.772 0.978 -0.951 -0.997 1
N 0.136 0.064 0.211 0.642 -0.217 -0.300 0.085 -0.009 1
Ash -0.127 -0.056 -0.202 -0.648 0.225 0.292 -0.093 0.017 -0.999 1
Increment 0.955 0.974 0.930 -0.860 0.998 -0.892 -0.997 0.988 -0.162 0.170
Parameter Chl a Chl b Car Chl a /
Chl b
Chl (a+b) /
Car
Car /
Chl (a+b) Ca P N Ash
Chl b 0.974 1
Car 0.990 0.996 1
Chl a / Chl b -0.575 -0.744 -0.683 1
Chl (a+b) / Car 0.847 0.707 0.766 -0.057 1
Car / Chl (a+b) -0.848 -0.707 -0.766 0.053 -1 1
Ca -0.991 -0.996 -0.999 0.679 -0.769 0.769 1
P 0.968 0.887 0.924 -0.352 0.953 -0.953 -0.926 1
N -0.996 -0.953 -0.976 0.507 -0.888 0.888 0.977 -0.985 1
Ash -0.999 -0.976 -0.991 0.581 -0.844 0.844 0.992 -0.966 0.996 1
Increment 0.883 0.756 0.810 -0.125 0.997 -0.997 -0.813 0.972 -0.918 -0.880
Table 5. Correlation of investigated parameters of Astragalus ponticus.
Table 4. Correlation of investigated parameters of Astragalus glycyphyllos.
https://doi.org/10.3906/biy-0805-1
https://doi.org/10.1146/annurev.pp.37.060186.000521
https://doi.org/10.1146/annurev.pp.37.060186.000521
https://doi.org/10.4141/P04-174
https://doi.org/10.4141/P04-174
https://doi.org/10.1142/S0192415X16500014
https://doi.org/10.1142/S0192415X16500014
84 Plant Introduction • 87/88
O. Bondarchuk, O. Vergun, O. Shymanska, V. Fishchenko, D. Rakhmetov
Babich, O., Prosekov, A., Zaushintsena, A.,
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Plant Introduction • 87/88 85
Accumulation of ash and photosynthetic pigments in the raw material of Astragalus
Накопичення золи та фотосинтетичних пігментів у рослинній сировині видів
роду Astragalus
Олександр Бондарчук *, Олена Вергун, Оксана Шиманська, Валентина Фіщенко, Джамал Рахметов
Національний ботанічний сад імені М.М. Гришка НАН України, вул. Тимірязєвська, 1, м. Київ, 01014,
Україна; * bondbiolog@gmail.com
Досліджено п’ять видів роду Astragalus (A. cicer, A. falcatus, A. galegiformis, A. glycyphyllos та A. ponticus) з
відділу культурної флори Національного ботанічного саду імені М.М. Гришка НАН України. Рослинну
сировину для досліджень відбирали у період бутонізації, квітування та плодоношення. Після чого,
визначали у ній вміст золи, кальцію, фосфору, азоту та фотосинтетичних пігментів.
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до 0,14 %, азоту – від 1,93 до 2,48 % залежно від фази розвитку. Вміст хлорофілу a становив від 0,901
до 2,475 мг/г, хлорофілу b – від 0,190 до 0,676 мг/г, а каротиноїдів – від 0,496 до 1,129 мг/г.
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86 Plant Introduction • 87/88
O. Bondarchuk, O. Vergun, O. Shymanska, V. Fishchenko, D. Rakhmetov
Таким чином, накопичення золи та її компонентів у фітосировині досліджених видів варіювало
і залежало від періоду розвитку та видових особливостей. Вміст фотосинтетичних пігментів
корелював із вмістом фосфору (r = 0,887–0,999) та з приростом рослин (r = 0,756–0,998) для усіх
досліджених видів. Отримані відомості можуть бути використані для дослідження продуктивності
цих рослин та під час їх культивування.
Ключові слова: Astragalus, рослинна сировина, вміст золи, фотосинтетичні пігменти, кореляція
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| id | oai:ojs2.plantintroduction.org:article-1567 |
| institution | Plant Introduction |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2025-07-17T12:53:44Z |
| publishDate | 2020 |
| publisher | M.M. Gryshko National Botanical Garden of the NAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | wwwplantintroductionorg/0d/67c4e2ab2d58fe6054ea8e7b9773a40d.pdf |
| spelling | oai:ojs2.plantintroduction.org:article-15672023-08-26T20:39:33Z Accumulation of ash and photosynthetic pigments in the raw material of Astragalus Накопичення золи та фотосинтетичних пігментів у рослинній сировині видів роду Astragalus Bondarchuk, Oleksandr Vergun, Olena Shymanska, Oksana Fishchenko, Valentyna Rakhmetov, Dzhamal Five Astragalus species (A. cicer, A. falcatus, A. galegiformis, A. glycyphyllos, and A. ponticus) grown at the M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine were investigated. Plant raw material was collected at different ontogenetic stages, including budding, flowering, and fruiting. After that, the content of ash, calcium, phosphorus, nitrogen, and photosynthetic pigments was analyzed and statistically processed.The content of ash varied from 3.12 to 5.22 %, calcium – from 0.31 to 0.91 %, phosphorus – from 0.07 to 0.14 %, and nitrogen – from 1.93 to 2.48 % depending on the stage of growth. The content of chlorophyll a varied from 0.901 to 2.475 mg/g FW (fresh weight), chlorophyll b – from 0.190 to 0.676 mg/g FW, and carotenoids – from 0.496 to 1.129 mg/g FW.Thus, the accumulation of ash and its components in the raw material of the investigated species depended on the period of growth and certain species. Content of photosynthetic pigments correlated with the content of phosphorus (r = 0.887–0.999) and with plant increment (r = 0.756–0.998) for all investigated species. Obtained data can be applied to analyze productivity and during the cultivation of these plants. Досліджено п’ять видів роду Astragalus (A. cicer, A. falcatus, A. galegiformis, A. glycyphyllos та A. ponticus) з відділу культурної флори Національного ботанічного саду імені М.М. Гришка НАН України. Рослинну сировину для досліджень відбирали у період бутонізації, квітування та плодоношення. Після чого, визначали у ній вміст золи, кальцію, фосфору, азоту та фотосинтетичних пігментів.Вміст золи у фітосировині варіював від 3,12 до 5,22 %, кальцію – від 0,31 до 0,91 %, фосфору – від 0,07 до 0,14 %, азоту – від 1,93 до 2,48 % залежно від фази розвитку. Вміст хлорофілу a становив від 0,901 до 2,475 мг/г, хлорофілу b – від 0,190 до 0,676 мг/г, а каротиноїдів – від 0,496 до 1,129 мг/г.Таким чином, накопичення золи та її компонентів у фітосировині досліджених видів варіювало і залежало від періоду розвитку та видових особливостей. Вміст фотосинтетичних пігментів корелював із вмістом фосфору (r = 0,887–0,999) та з приростом рослин (r = 0,756–0,998) для усіх досліджених видів. Отримані відомості можуть бути використані для дослідження продуктивності цих рослин та під час їх культивування. M.M. Gryshko National Botanical Garden of the NAS of Ukraine 2020-12-30 Article Article application/pdf https://www.plantintroduction.org/index.php/pi/article/view/1567 10.46341/PI2020031 Plant Introduction; No 87/88 (2020); 76-86 Інтродукція Рослин; № 87/88 (2020); 76-86 2663-290X 1605-6574 10.46341/PI87-88 en https://www.plantintroduction.org/index.php/pi/article/view/1567/1502 Copyright (c) 2020 Oleksandr Bondarchuk, Olena Vergun, Oksana Shymanska, Valentyna Fishchenko, Dzhamal Rakhmetov http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Bondarchuk, Oleksandr Vergun, Olena Shymanska, Oksana Fishchenko, Valentyna Rakhmetov, Dzhamal Накопичення золи та фотосинтетичних пігментів у рослинній сировині видів роду Astragalus |
| title | Накопичення золи та фотосинтетичних пігментів у рослинній сировині видів роду Astragalus |
| title_alt | Accumulation of ash and photosynthetic pigments in the raw material of Astragalus |
| title_full | Накопичення золи та фотосинтетичних пігментів у рослинній сировині видів роду Astragalus |
| title_fullStr | Накопичення золи та фотосинтетичних пігментів у рослинній сировині видів роду Astragalus |
| title_full_unstemmed | Накопичення золи та фотосинтетичних пігментів у рослинній сировині видів роду Astragalus |
| title_short | Накопичення золи та фотосинтетичних пігментів у рослинній сировині видів роду Astragalus |
| title_sort | накопичення золи та фотосинтетичних пігментів у рослинній сировині видів роду astragalus |
| url | https://www.plantintroduction.org/index.php/pi/article/view/1567 |
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