Гіпоглікемічний ефект полісахаридів, виділених з пагонів Actinidia arguta
Diabetes mellitus can result in hyperglycemia caused by insufficient insulin secretion or insulin resistance. As such, plant extracts that exert a hypoglycemic effect with limited side effects are of interest to the medical and healthcare fields. The hypoglycemic effect of polysaccharides extracted...
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Plant Introduction| _version_ | 1860145125809717248 |
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| author | Niu, Qiang Shen, Jian Li, Lili Zhang, Huiming Zaimenko, Natalia Skrypchenko, Nadiia Tian, Lijuan Sun, Honglai Wang, Lihong Liu, Dejiang |
| author_facet | Niu, Qiang Shen, Jian Li, Lili Zhang, Huiming Zaimenko, Natalia Skrypchenko, Nadiia Tian, Lijuan Sun, Honglai Wang, Lihong Liu, Dejiang |
| author_sort | Niu, Qiang |
| baseUrl_str | https://www.plantintroduction.org/index.php/pi/oai |
| collection | OJS |
| datestamp_date | 2023-08-26T20:38:45Z |
| description | Diabetes mellitus can result in hyperglycemia caused by insufficient insulin secretion or insulin resistance. As such, plant extracts that exert a hypoglycemic effect with limited side effects are of interest to the medical and healthcare fields. The hypoglycemic effect of polysaccharides extracted from the branches of Actinidia arguta was explored in mice in this study. Sixty male Kunming mice were subsequently randomly assigned to one of six groups. The body weight, fasting blood glucose level, serum lipids, and oxidative stress parameters were assessed weekly during the 28-day study period. Pancreatic tissue from sacrificed mice was harvested at the end of the study and dissected for analysis. Polysaccharide AABP3 prevented body weight loss and decreased the fasting blood glucose level in diabetic mice compared with control mice. It also had a beneficial effect on serum dyslipidemia and oxidative stress parameters and was comparable in its protective effect to metformin. Histopathological examination of the pancreas revealed that AABP3 could protect and ameliorate pancreatic damage that may occur in diabetes mellitus in mice. AABP3 may be considered a potential candidate for developing a functional food or natural product for treating diabetes and its complications. |
| doi_str_mv | 10.46341/PI2022008 |
| first_indexed | 2025-07-17T12:54:05Z |
| format | Article |
| fulltext |
© The Authors. This content is provided under CC BY 4.0 license.
Plant Introduction, 95/96, 44–52 (2022)
RESEARCH ARTICLE
Hypoglycemic effect of polysaccharides isolated from shoots of Actinidia
arguta
Qiang Niu 1, 2, Jian Shen 2, Lili Li 2, Huiming Zhang 2, Natalia Zaimenko 2, 3, Nadiia Skrypchenko 2, 3,
Lijuan Tian 2, Honglai Sun 2, Lihong Wang 1, Dejiang Liu 2, *
1 College of Pharmacy, Jiamusi University, Xuefu str. 258, 154007 Jiamusi, China
2 China-Ukraine Agriculture & Forestry Technology Development and Application International Cooperation Joint Lab, Xuefu str. 258,
154007 Jiamusi, China; * Liudejiang2004@163.com
3 M.M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine, Tymiryazevska str. 1, 01014 Kyiv, Ukraine
Received: 14.03.2022 | Accepted: 13.07.2022 | Published online: 01.08.2022
Abstract
Diabetes mellitus can result in hyperglycemia caused by insufficient insulin secretion or insulin resistance.
As such, plant extracts that exert a hypoglycemic effect with limited side effects are of interest to the
medical and healthcare fields. The hypoglycemic effect of polysaccharides extracted from the branches of
Actinidia arguta was explored in mice in this study. Sixty male Kunming mice were subsequently randomly
assigned to one of six groups. The body weight, fasting blood glucose level, serum lipids, and oxidative
stress parameters were assessed weekly during the 28-day study period. Pancreatic tissue from sacrificed
mice was harvested at the end of the study and dissected for analysis. Polysaccharide AABP3 prevented
body weight loss and decreased the fasting blood glucose level in diabetic mice compared with control
mice. It also had a beneficial effect on serum dyslipidemia and oxidative stress parameters and was
comparable in its protective effect to metformin. Histopathological examination of the pancreas revealed
that AABP3 could protect and ameliorate pancreatic damage that may occur in diabetes mellitus in mice.
AABP3 may be considered a potential candidate for developing a functional food or natural product for
treating diabetes and its complications.
Keywords: Actinidia arguta, polysaccharides, hypoglycemic effect, diabetes mellitus, streptozotocin
https://doi.org/10.46341/PI2022008
UDC 581.524.1
Authors’ contributions: Qiang Niu and Jian Shen for Co-author wrote the manuscript and performed the experiments. Lili Li, Huiming
Zhang and Nadiia Skrypchenko analyzed and interpreted data, created figure. Honglai Sun performed the animal experiments.
Dejiang Liu, Lihong Wang and Natalia Zaimenko designed the experiments, revised and proved the manuscript.
Funding: This work was supported by the central government supports the reform and development fund of local colleges and
universities for Outstanding Young Talents Project (Grant Nr 2020YQ09).
Competing Interests: The authors have no conflicts of interest to declare.
Statement of ethics: Sixty male Kunming mice (weighing 20 ± 2 g) were purchased from Yanbian University. The license number is
SCXK (Ji) 2017-0003.
Introduction
Diabetes mellitus (DM) is a group of metabolic
diseases characterized by hyperglycemia
caused by insufficient insulin secretion,
insulin resistance, or both (Wang et al., 2017).
Chronic hyperglycemia in diabetic patients
can result in damage to the eyes, kidneys,
https://creativecommons.org/licenses/by/4.0/
https://orcid.org/0000-0003-0889-5390
https://orcid.org/0000-0003-1378-5727
https://orcid.org/0000-0001-9738-2794
https://orcid.org/0000-0002-9561-8545
https://orcid.org/0000-0003-2379-1223
https://orcid.org/0000-0002-1233-9920
https://orcid.org/0000-0001-7288-2602
https://orcid.org/0000-0001-9925-4710
https://orcid.org/0000-0001-9915-8868
https://orcid.org/0000-0002-1419-2133
Plant Introduction • 95/96 45
Hypoglycemic effect of polysaccharides isolated from shoots of Actinidia arguta
heart, blood vessels, and other organs, leading
to dysfunction (Ye et al., 2019). According to
the different pathogeneses of diabetes, the
condition can be divided into four main types:
type I diabetes, type II diabetes, gestational
diabetes, and secondary diabetes. Among
them, type II diabetes accounts for about
90 % of diabetes cases (Stumvoll et al., 2005;
Kahn et al., 2006). Among patients suffering
with type II diabetes, insulin may be secreted
but the quantity is relatively insufficient due
to insulin resistance, meaning the secreted
insulin cannot play an effective role and an
increase in blood glucose results. Because
patients with this kind of diabetes can produce
insulin themselves, they can control their
blood sugar through diet or oral hypoglycemic
drugs without requiring insulin treatment
(Drucker & Nauck, 2006; Ridderstrale
& Groop, 2009). At present, insulin and
some oral hypoglycemic drugs are mainly
used in the clinical treatment of diabetes,
including biguanides, thiazolidinediones, and
sulfonylureas. However, these drugs have
some side effects, such as hypoglycemia
and gastrointestinal disorders (Chen et al.,
2019). Large number of natural drugs have
been studied for the treatment of diabetes
and reported in the literature with promised
results achieved (Fu et al., 2012; Cao, 2013;
Xu et al., 2014; Chen et al., 2016; Wang et al.,
2017; Liu et al., 2017). An increasing number
of plant polysaccharides have been studied
and found to have a good hypoglycemic
effect, as well as low toxicity and limited side
effects, in the search for effective and safe
treatment options for DM. As a result, these
polysaccharides have become a hotspot in
medicine and healthcare (Zhang et al., 2016).
Actinidia arguta (Siebold & Zucc.) Planch.
ex Miq. belongs to the Actinidiaceae Engl. &
Gilg family, and is a perennial and deciduous
liana (Li et al., 2019). It is called the “king of
fruits” because of its high content of amino
acids, minerals, antioxidants, vitamin C
and dietary fiber (Skrypchenko, 2017; Shin
et al., 2019). The hypoglycemic effect of
polysaccharides extracted from the branches
of A. arguta has not been reported in detail.
As such, in this study, we explored the impact
of A. arguta polysaccharides on the body
weight, fasting blood glucose (FBG) level,
blood lipid index, and serum biochemical
index of diabetic mice.
Material and methods
Materials and chemicals
Total cholesterol (TC), triglyceride (TG), low-
density lipoprotein-C (LDL-C), high-density
lipoprotein-C (HDL-C), malondialdehyde
(MDA) and superoxide dismutase(SOD) were
purchased from Nanjing Jiancheng Bio-
engineering Institute (Nanjing, Сhina). All
the other reagents and solvents used for the
extraction and isolation were of analytical
grade and purchased from local firms.
Preparation of polysaccharides
The branches of A. arguta were offered
by Agriculture and Forestry Experimental
Practice Base of Jiamusi University and were
authenticated by senior experimentalist De-
jiang Liu in Jiamusi University. The conditions
for polysaccharide extraction were as follows:
liquid–material ratio, 29 mL g-1; extraction
temperature, 80 °C; extraction time, 128 min.
After centrifugation at 5000 rpm for 10 min,
the supernatant was concentrated to a certain
volume under reduced pressure using a rotary
evaporator, and precipitation was achieved
with 80 % (v/v) ethanol/water overnight.
The resulting precipitates were collected
and freeze-dried. The crude polysaccharides
were deproteinized via the trichloroacetic
acid method and then decolorized by an
AB-8 macroporous adsorption resin. The
crude polysaccharides were then applied to a
diethylaminoethyl (DEAE-52) cellulose anion-
exchange column and eluted with distilled
water followed by gradient solutions (0.1, 0.2,
and 0.3 mol L-1 NaCl) at a flow rate of 1 mL min-1.
The eluents were collected in 5 mL fractions,
and the polysaccharides were determined by
the phenol-sulfuric acid method. An elution
curve was drawn with the tube number
(x-axis) and absorbance (y-axis). The resulting
four polysaccharides were named A. arguta
branch polysaccharide (AABP): AABP1, AABP2,
AABP3, and AABP4. AABP3 was the major
polysaccharide and was thus selected to study
its hypoglycemic effect in mice.
Experimental animals
Sixty male Kunming mice (weighing 20 ± 2 g)
were purchased from Yanbian University. The
license number is SCXK (Ji) 2017-0003. All
46 Plant Introduction • 95/96
Q. Niu, J. Shen, L. Li et al.
mice had free access to tap water and were
provided a standard laboratory diet prior to
the experiment. They were housed at 22 ± 3 °C
with a 12 h light-dark cycle.
Experimental design
The mice were randomly divided into six
groups (10 mice per group). Group 1: mice
were intragastrically administered distilled
water every day during the experimental
period (normal control group, NC). Group 2:
streptozotocin (STZ)-induced diabetic
mice were intragastrically administered
distilled water (model control group, MC).
Group 3: STZ-induced diabetic mice were
intragastrically administered 10 mg kg-1 body
weight AABP3 (low-dose group, AABP3-L).
Group 4: STZ-induced diabetic mice were
intragastrically administered 20 mg kg-1
body weight AABP3 (medium-dose group,
AABP3-M). Group 5: STZ-induced diabetic
mice were intragastrically administered 40 mg
kg-1 body weight AABP3 (high-dose group,
AABP3-H). Group 6: STZ-induced diabetic
mice were intragastrically administered
15 mg kg-1 body weight metformin (positive
control group, PC).
The NC mice were fed a normal diet for
four weeks after one week of acclimatization
prior to the onset of the study. Mice in groups
2 to 6 were fed a high-fat and high-sugar
diet for four weeks. After this time, fasted
mice were given an intraperitoneal injection
of STZ dissolved in cold citrate buffer once
at a dose of 80 mg kg-1 body weight. NC mice
received an equal volume of cold citrate buffer
injection. The blood glucose levels of the mice
were measured by the collection of a drop
of blood from the tip of the tail, which was
subsequently tested with a GA-3 blood glucose
meter. Mice with a blood glucose level greater
than 11.1 mmol L-1 were assumed to be diabetic
three days after intraperitoneal injection.
Body weight and FBG levels were monitored
weekly throughout the whole experimental
period (28 days). After 12 h of fasting following
the last intragastric gavage, blood samples
were collected from the eye vein by removal
of the eyeball. Serum was then harvested by
centrifuging the blood samples at 3500 rpm
for 10 min and stored at –20 °C for further
assay. The pancreas of mice were harvested
and dissected for analysis.
Determination of serum lipid levels
The serum triglyceride (TC), total cholesterol
(TG), low-density lipoprotein-cholesterol
(LDL-C) and high-density lipoprotein-
cholesterol (HDL-C) levels were tested
following the manufacturers’ instructions.
Determination of serum biochemical indexes
The levels of malondialdehyde (MDA) and
superoxide dismutase (SOD) in serum
supernatant were tested following the
manufacturers’ instructions.
Histopathological analysis
Pancreatic tissue fixed in a 10 % formalin
solution was embedded in paraffin using
a standard tissue-embedding procedure.
Paraffin-embedded sections were stained with
hematoxylin and eosin (H&E) for histological
examination. All slices were visualized and
captured using an inverted optical microscope
XDS-18 (Beijing Rongxing Guangheng
technology Co., LTD).
Statistical analysis
All the experimental data were expressed
as mean ± standard deviation (SD) in each
group. Comparisons between the groups were
performed by one-way analysis of variance
(ANOVA) and the least significant difference
(LSD) test. Data with p < 0.05 were considered
statistically significant.
Results
Effect of AABP3 on body weight
Reduction of body weight is a symptom
associated with DM; therefore, the body
weight of mice in each group was monitored
every week during the 28-day study period.
The experimental results are shown in Table 1.
There was no significant difference in body
weight between the mice in all test groups
(20 ± 2 g; p > 0.05) before the study. However,
all STZ-induced diabetic groups presented a
significant loss of body weight compared to
the NC group prior to gavage (p < 0.01). The
AABP3-L, AABP3-M, and AABP3-H groups
showed a significant increase in body weight
(p < 0.01) compared with the MC group. The
effect of AABP3-H on the body weight of
Plant Introduction • 95/96 47
Hypoglycemic effect of polysaccharides isolated from shoots of Actinidia arguta
diabetic mice was similar to that observed in
the PC group (p > 0.05). The data indicate that
AABP3 possesses the ability to protect and
ameliorate body weight loss in diabetic mice.
Effect of AABP3 on FBG levels
Hyperglycemia is characteristic of DM and
the FBG level in serum is usually monitored
to reflect changes in blood glucose. The FBG
levels recorded in this study are shown in
Table 2. There was no significant difference
in the FBG levels of mice in all test groups
(p > 0.05) before embarking on the experiment.
However, compared with the NC group, all
STZ-induced diabetic groups presented a
significant increase in FBG prior to gavage
(p < 0.01). Compared with the MC group, the
AABP3-L, AABP3-M, and AABP3-H groups
showed a significant decrease in FBG (p < 0.01).
The effect of AABP3-H on the FBG levels of
diabetic mice was similar to that of the PC
group (p > 0.05). The data indicate that AABP3
possesses the ability to lower FBG in diabetic
mice.
Effect of AABP3 on blood lipids
Abnormal lipid metabolism leads to an increase
in TC, TG and LDL-C, and a decrease in HDL-C
in the diabetic condition (Rangika et al., 2015;
Akindele et al., 2015; Emordi et al., 2016). The
results of the blood lipid analysis performed
in this study are shown in Table 3. TC, TG
and LDL-C levels were significantly increased
in the MC group compared to the NC group
(MC vs NC: 7.29 ± 0.09 vs 3.14 ± 0.10 mmol L-1,
2.42 ± 0.12 vs 0.92 ± 0.08 mmol L-1 and 0.84 ± 0.03
vs 0.39 ± 0.03 mmol L-1, respectively; p < 0.01),
while HDL-C was significantly decreased
from 1.13 ± 0.06 mmol L-1 in the NC group to
0.42 ± 0.08 mmol L-1 in the MC group (p < 0.01).
Test groups 7 days (g) 14 days (g) 21 days (g) 28 days (g)
NC 44.25 ± 1.27 48.00 ± 1.33 49.90 ± 0.97 52.00 ± 0.88
MC 41.00 ± 1.58 a 37.60 ± 3.27 a 32.75 ± 2.87 a 30.80 ± 2.50 a
AABP3-L 40.85 ± 1.27 42.65 ± 1.53 43.95 ± 1.70 45.90 ± 2.22 b
AABP3-M 41.00 ± 1.63 43.40 ± 1.54 45.05 ± 1.59 47.35 ± 1.53 b
AABP3-H 41.65 ± 1.47 44.50 ± 2.04 46.30 ± 1.70 49.40 ± 1.33 bc
PC 40.80 ± 2.78 45.10 ± 2.28 47.35 ± 1.76 49.90 ± 1.29
Table 1. The effect of AABP3 on the body weight of diabetic mice (values are expressed as mean ± SD;
n = 10 per group).
Note. a – compared with the NC group: p < 0.01; b – compared with the MC group: p < 0.01; c – compared
with the PC group: p > 0.05.
Test groups 7 days (g) 14 days (g) 21 days (g) 28 days (g)
NC 4.56 ± 0.52 5.17 ± 0.46 5.30 ± 0.48 5.43 ± 0.55
MC 25.88 ± 3.79 a 27.82 ± 2.55 a 28.58 ± 2.88 a 29.23 ± 2.33 a
AABP3-L 23.88 ± 3.95 21.17 ± 3.34 19.95 ± 3.43 18.43 ± 2.55 b
AABP3-M 25.21 ± 3.88 21.58 ± 3.81 19.40 ± 2.78 17.91 ± 3.01 b
AABP3-H 22.84 ± 4.12 18.82 ± 4.78 15.56 ± 4.19 10.71 ± 2.99 bc
PC 22.85 ± 2.79 17.48 ± 3.20 14.51 ± 3.83 10.42 ± 2.22
Table 2. The effect of AABP3 on the FBG levels of diabetic mice (values are expressed as mean ± SD; n = 10
per group).
Note. a – compared with the NC group: p < 0.01; b – compared with the MC group: p < 0.01; c – compared
with the PC group: p > 0.05.
48 Plant Introduction • 95/96
Q. Niu, J. Shen, L. Li et al.
After four weeks of gavage, the TC, TG, and
LDL-C levels in mice treated with AABP3-H
were significantly decreased to 4.37 ± 0.12,
1.23 ± 0.08, and 0.54 ± 0.04 mmol L-1 (p < 0.01),
respectively, while HDL-C was significantly
increased to 0.98 ± 0.06 mmol L-1 compared
with the MC group (p < 0.01). The effect of
AABP3-H on blood lipid changes associated
with DM in mice was similar to that of the PC
(p > 0.05). The data indicate that AABP3 had a
beneficial effect on dyslipidemia.
Effect of AABP3 on oxidative stress
parameters in the serum
Recently, many studies have demonstrated
that DM is typically associated with the
increased generation of free radicals or an
impaired antioxidant defense mechanism,
resulting in diabetes-induced pathological
consequences (Liu et al., 2014; Pan et al., 2014;
Adefegha et al., 2014). As such, we investigated
the changes in MDA and SOD in this study,
and the results are shown in Fig. 1. The
level of MDA was significantly increased
from 6.07 ± 0.25 nmol L-1 in the NC group to
11.90 ± 0.82 mmol L-1 in the MC group (p < 0.01),
while the level of SOD was significantly
decreased from 22.78 ± 0.26 U mL-1 in the
NC group to 20.71 ± 0.16 U mL-1 in the MC
group (p < 0.01). The level of MDA in mice
administered AABP3-H was significantly
decreased to 6.49 ± 0.56 nmol L-1 (p < 0.01)
after four weeks of gavage, while the level
of SOD was increased considerably to
22.43 ± 0.14 U mL-1 compared with the MC
group (p < 0.01). The effect of AABP3-H on the
oxidative stress changes observed in diabetic
mice was better than that of the PC. The data
indicate that AABP3 had a beneficial impact
on the serum oxidative stress parameters of
diabetic mice.
Test groups TC (mmol L-1) TG (mmol L-1) HDL-C (mmol L-1) LDL-C (mmol L-1)
NC 3.14 ± 0.10 0.92 ± 0.08 1.13 ± 0.06 0.39 ± 0.03
MC 7.29 ± 0.09 a 2.42 ± 0.12 a 0.42 ± 0.08 a 0.84 ± 0.03 a
AABP3-L 6.01 ± 0.19 b 1.75 ± 0.11 b 0.67 ± 0.08 b 0.72 ± 0.05 b
AABP3-M 5.27 ± 0.23 b 1.45 ± 0.12 b 0.77 ± 0.65 b 0.63 ± 0.05 b
AABP3-H 4.37 ± 0.12 bc 1.23 ± 0.08 bc 0.98 ± 0.06 b 0.54 ± 0.04 b
PC 3.67 ± 0.15 1.13 ± 0.09 1.00 ± 0.10 0.48 ± 0.04
Table 3. The effect of AABP3 on the blood lipids of diabetic mice (values are expressed as mean ± SD; n = 5
per group).
Note. a – compared with the NC group: p < 0.01; b – compared with the MC group: p < 0.01; c – compared
with the PC group: p > 0.05.
Figure 1. The effect of AABP3 on serum biochemical indexes in diabetic mice after treatment with FPLP for
four weeks (values are presented as mean ± SD (n = 5): a – p < 0.05 compared with NC group; b – p < 0.05
compared with MC group; c – p > 0.05 compared with PC group).
a
ab
b ababc
ab ab abc ab
a
Plant Introduction • 95/96 49
Hypoglycemic effect of polysaccharides isolated from shoots of Actinidia arguta
Histopathological observation of the
pancreas
Pancreas slices observed via histopathology
are shown in Fig. 2. The architecture of
the pancreatic islets in the NC group
was characterized by clear, round or oval
boundaries and the islet cells had normal
morphology and were arranged closely.
However, the pancreatic islets in the MC
group exhibited irregular shapes and the islet
cells were extensively destroyed. AABP3-H
improved the detrimental changes observed
in the pancreas tissue of diabetic mice
compared with the MC group. The islet cells
had normal morphology and the empty areas
were reduced in size. This result suggests that
AABP3 may protect and ameliorate pancreatic
damage in DM.
Discussion
Diabetes is a serious health issue worldwide,
producing significant morbidity and mortality,
and there is no route to cure diabetes
completely. Patients with type II diabetes
can control their blood sugar level by oral
administration of some hypoglycemic drugs,
but currently, some hypoglycemic drugs can
cause toxic and other side effects such as
hypoglycemia and gastrointestinal diseases.
Therefore, developing new drugs with good
curative effects and little side effects is of
great significance. At present, STZ and alloxan
are the main chemical drugs for inducing type
II diabetes. After injecting alloxan, blood sugar
may recover, while when injecting STZ, mice
will die if the dose is too high. Therefore, this
experiment can simulate diabetes caused by
environmental factors by feeding a high-fat
and high-sugar feed combined with low-dose
intraperitoneal injection of STZ to induce a type
II diabetes mouse model, artificially destroying
islet beta cells to damage islets, and the
induction method is suitable for establishing
a type II diabetes model. In this experiment,
after 28 days of gastric administration of
AABP3 in mice with type II diabetes, the
symptoms of “three more and one less”
diabetes in mice in the administration group
were recovered, and the fasting blood glucose
level was significantly reduced. The contents of
TC, TG, LDL-C, and HDL-C in the four indexes
of blood lipid of mice in the administration
group were significantly reduced, indicating
that the abnormal phenomenon of blood lipid
in diabetic mice was improved. The content
of MDA in serum decreased obviously, and
the content of SOD increased obviously,
which indicated that AABP3 could reduce
blood sugar by inhibiting peroxidation. It
was found that AABP3 high dose group has
a certain protective effect on the pancreas
of STZ-induced diabetic mice through
histological observation. It is concluded
that AABP3 regulates the blood sugar level
and lipid metabolism of diabetic mice and
alleviates insulin resistance, thus playing
a certain protective role on the pancreas
of mice. AABP3-H had the most significant
therapeutic effect in DM and showed similar
results to the PC, suggesting AABP3-H acts
in a dose-dependent manner. It can thus be
concluded that AABP3 may be considered a
potential candidate for developing a functional
food or natural product for treating DM
and its complications. Polysaccharides and
other hypoglycemic drugs obtained from
natural plants have good effects, low toxic
and side effects, and complex mechanisms of
action. Subsequent experiments can further
explore the hypoglycemic mechanism of
this polysaccharide on diabetic mice and
explore some toxic and side effects of the
polysaccharide. In the pruning process of
A. arguta, a large number of branches will be
discarded, and the discarded branches will be
recycled for utilization. The polysaccharide in
the branches will be extracted and purified,
and its hypoglycemic mechanism will be
explored. The added value of A. arguta can be
increased, which can not only avoid waste but
also reduce pollution and achieve the purpose
of waste utilization.
Conclusions
In summary, according to the present findings,
our study demonstrated that AABP3 is the main
fraction of polysaccharides extracted from the
branches of A. arguta. In in vivo assays, AABP3
revealed a potent hypoglycemic effect on STZ-
induced diabetic rats for its ability to reduce
glucose levels and ameliorate lipid metabolism
and oxidative stress. Further studies in animal
models and human volunteers need to be
done to substantiate these findings. AABP3
50 Plant Introduction • 95/96
Q. Niu, J. Shen, L. Li et al.
BA
DC
FE
Figure 2. Histopathological study of pancreas tissue from mice (H&E staining, ×400 magnification): A – NC
group; B – MC group; C – AABP3-L group; D – AABP3-M group; E – AABP3-H group; F – PC group.
may be considered a potential candidate for
developing functional food or natural products
for treating diabetes and its complications.
Therefore, an important stage of further
research is the study of the qualitative and
quantitative characteristics of polysaccharides
extracted from the shoots of A. arguta, for the
development of methods of quality control of
raw materials at the creation of drugs with a
hypoglycemic effect.
Plant Introduction • 95/96 51
Hypoglycemic effect of polysaccharides isolated from shoots of Actinidia arguta
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Гіпоглікемічний ефект полісахаридів, виділених з пагонів Actinidia arguta
Цян Ніу 1, 2, Цзянь Шень 2, Лілі Лі 2, Хуймін Чжан 2, Наталія Заіменко 2, 3, Надія Скрипченко 2, 3, Ліцзюань
Тянь 2, Хунлай Сун 2, Ліхон Ван 1, Децзян Лю 2, *
1 Фармацевтичний коледж, Університет Цзямусі, вул. Сюефу, 258, Цзямусі, Хейлунцзян, 154007, Китай
2 Китайсько-українська спільна лабораторія розвитку та застосування технологій сільського та
лісового господарства, Університет Цзямусі, вул. Сюефу, 258, Цзямусі, Хейлунцзян, 154007, Китай;
* Liudejiang2004@163.com
3 Національний ботанічний сад імені М.М. Гришка НАН України, вул. Тімірязєвська, 1, Київ, 01014,
Україна
Цукровий діабет може призвести до гіперглікемії, спричиненої недостатньою секрецією
інсуліну або інсулінорезистентністю. Тому рослинні екстракти, які мають гіпоглікемічний ефект
з обмеженими побічними ефектами, представляють інтерес для медицини та охорони здоров’я.
У цьому дослідженні на мишах досліджували гіпоглікемічний ефект полісахаридів, виділених з
пагонів Actinidia arguta. Шістдесят самців мишей Куньмін були випадковим чином розподілені в
шість груп по десять особин. Протягом 28-денного періоду дослідження щотижня оцінювали масу
тіла, рівень глюкози в крові натщесерце, ліпіди сироватки та параметри окисного стресу. В кінці
дослідження були відібрані зразки тканини підшлункової залози дослідних мишей для подальшого
аналізу. Встановлено, що полісахарид AABP3 запобігав втраті маси тіла мишей та знижував рівень
глюкози в крові натщесерце у мишей з діабетом порівняно з контролем. Він також мав сприятливий
вплив на сироваткову дисліпідемію та параметри окисного стресу та за своїм захисним ефектом
був подібним до метформіну. Гістопатологічне дослідження підшлункової залози показало, що
полісахарид AABP3 може захищати та зменшувати пошкодження підшлункової залози, які можуть
виникати при цукровому діабеті у мишей. AABP3 можна розглядати як потенційного кандидата для
розробки функціонального харчового або натурального продукту для лікування діабету та його
ускладнень.
Ключові слова: Actinidia arguta, полісахариди, гіпоглікемічний ефект, цукровий діабет, стрептозотоцин
https://doi.org/10.1016/j.bioorg.2019.102942
https://doi.org/10.1016/j.bioorg.2019.102942
https://doi.org/10.3390/ijms17111810
|
| id | oai:ojs2.plantintroduction.org:article-1608 |
| institution | Plant Introduction |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2025-07-17T12:54:05Z |
| publishDate | 2022 |
| publisher | M.M. Gryshko National Botanical Garden of the NAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | wwwplantintroductionorg/80/4a7015339dc0b0cdd6f44d9028520380.pdf |
| spelling | oai:ojs2.plantintroduction.org:article-16082023-08-26T20:38:45Z Hypoglycemic effect of polysaccharides isolated from shoots of Actinidia arguta Гіпоглікемічний ефект полісахаридів, виділених з пагонів Actinidia arguta Niu, Qiang Shen, Jian Li, Lili Zhang, Huiming Zaimenko, Natalia Skrypchenko, Nadiia Tian, Lijuan Sun, Honglai Wang, Lihong Liu, Dejiang Diabetes mellitus can result in hyperglycemia caused by insufficient insulin secretion or insulin resistance. As such, plant extracts that exert a hypoglycemic effect with limited side effects are of interest to the medical and healthcare fields. The hypoglycemic effect of polysaccharides extracted from the branches of Actinidia arguta was explored in mice in this study. Sixty male Kunming mice were subsequently randomly assigned to one of six groups. The body weight, fasting blood glucose level, serum lipids, and oxidative stress parameters were assessed weekly during the 28-day study period. Pancreatic tissue from sacrificed mice was harvested at the end of the study and dissected for analysis. Polysaccharide AABP3 prevented body weight loss and decreased the fasting blood glucose level in diabetic mice compared with control mice. It also had a beneficial effect on serum dyslipidemia and oxidative stress parameters and was comparable in its protective effect to metformin. Histopathological examination of the pancreas revealed that AABP3 could protect and ameliorate pancreatic damage that may occur in diabetes mellitus in mice. AABP3 may be considered a potential candidate for developing a functional food or natural product for treating diabetes and its complications. Цукровий діабет може призвести до гіперглікемії, спричиненої недостатньою секрецією інсуліну або інсулінорезистентністю. Тому рослинні екстракти, які мають гіпоглікемічний ефект з обмеженими побічними ефектами, представляють інтерес для медицини та охорони здоров’я. У цьому дослідженні на мишах досліджували гіпоглікемічний ефект полісахаридів, виділених з пагонів Actinidia arguta. Шістдесят самців мишей Куньмін були випадковим чином розподілені в шість груп по десять особин. Протягом 28-денного періоду дослідження щотижня оцінювали масу тіла, рівень глюкози в крові натщесерце, ліпіди сироватки та параметри окисного стресу. В кінці дослідження були відібрані зразки тканини підшлункової залози дослідних мишей для подальшого аналізу. Встановлено, що полісахарид AABP3 запобігав втраті маси тіла мишей та знижував рівень глюкози в крові натщесерце у мишей з діабетом порівняно з контролем. Він також мав сприятливий вплив на сироваткову дисліпідемію та параметри окисного стресу та за своїм захисним ефектом був подібним до метформіну. Гістопатологічне дослідження підшлункової залози показало, що полісахарид AABP3 може захищати та зменшувати пошкодження підшлункової залози, які можуть виникати при цукровому діабеті у мишей. AABP3 можна розглядати як потенційного кандидата для розробки функціонального харчового або натурального продукту для лікування діабету та його ускладнень. M.M. Gryshko National Botanical Garden of the NAS of Ukraine 2022-08-01 Article Article application/pdf https://www.plantintroduction.org/index.php/pi/article/view/1608 10.46341/PI2022008 Plant Introduction; No 95/96 (2022); 44-52 Інтродукція Рослин; № 95/96 (2022); 44-52 2663-290X 1605-6574 10.46341/PI95-96 en https://www.plantintroduction.org/index.php/pi/article/view/1608/1531 Copyright (c) 2022 Qiang Niu, Jian Shen, Lili Li, Huiming Zhang, Natalia Zaimenko, Nadiia Skrypchenko, Lijuan Tian, Honglai Sun, Lihong Wang, Dejiang Liu http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Niu, Qiang Shen, Jian Li, Lili Zhang, Huiming Zaimenko, Natalia Skrypchenko, Nadiia Tian, Lijuan Sun, Honglai Wang, Lihong Liu, Dejiang Гіпоглікемічний ефект полісахаридів, виділених з пагонів Actinidia arguta |
| title | Гіпоглікемічний ефект полісахаридів, виділених з пагонів Actinidia arguta |
| title_alt | Hypoglycemic effect of polysaccharides isolated from shoots of Actinidia arguta |
| title_full | Гіпоглікемічний ефект полісахаридів, виділених з пагонів Actinidia arguta |
| title_fullStr | Гіпоглікемічний ефект полісахаридів, виділених з пагонів Actinidia arguta |
| title_full_unstemmed | Гіпоглікемічний ефект полісахаридів, виділених з пагонів Actinidia arguta |
| title_short | Гіпоглікемічний ефект полісахаридів, виділених з пагонів Actinidia arguta |
| title_sort | гіпоглікемічний ефект полісахаридів, виділених з пагонів actinidia arguta |
| url | https://www.plantintroduction.org/index.php/pi/article/view/1608 |
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