Накопичення поживних речовин у підземній частині рослин видів роду Crambe L.
Objective – to study the accumulation of biochemical compounds in the underground part of the plants of the genus Crambe L. in the period of early spring in the conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. Plant material of this investigation – Cr...
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M.M. Gryshko National Botanical Garden of the NAS of Ukraine
2018
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Plant Introduction| _version_ | 1860120762955857920 |
|---|---|
| author | Vergun, O.M. Rakhmetov, D.B. Shymanska, O.V. Fishchenko, V.V. |
| author_facet | Vergun, O.M. Rakhmetov, D.B. Shymanska, O.V. Fishchenko, V.V. |
| author_sort | Vergun, O.M. |
| baseUrl_str | https://www.plantintroduction.org/index.php/pi/oai |
| collection | OJS |
| datestamp_date | 2019-11-11T08:15:12Z |
| description | Objective – to study the accumulation of biochemical compounds in the underground part of the plants of the genus Crambe L. in the period of early spring in the conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine.
Material and methods. Plant material of this investigation – Crambe species (Brassicaceae Burnett): C. cordifolia Steven (CCR), C. grandiflora DC. (CGR), C. juncea M.Bieb. (CJR), C. koktebelica (Junge) N. Busch (CKR), C. maritima L. (CMR), C. steveniana Rupr. (CSR), C. tataria Sebeok (CTR). As control were selected plants of Armoracia rusticana P. Gaerth., B. Mey et Scherb (ARR). All biochemical analyses were conducted using the underground part of plants in the period of early spring. The determination of absolutely dry matter was done according to A.I. Yermakov, total content of sugars, ascorbic acid, content of organic acids, tannins – according to V.P. Krishchenko, level of total ash – according to Z.M. Hrycajenko et al., concentration of calcium and phosphorus – according to H.N. Pochinok, antiradical activity of ethanol and water extracts – according to W. Brandt-Williams et al.
Results. Obtained data showed that content of dry matter ranged from 16.00 % (CMR) to 22.48 % (CCR), total content of sugar ranged from 11.03 % (CJR) to 46.48 % (CSR), content of ascorbic acid ranged from 192.85 mg% (CSR) to 483.45 mg% (CTR), level of tannins in range from 1.64 % (CGR) to 5.12 % (CJR), content of organic acids ranged from 2.28 % (CSR) to 3.64 % (CJR), content of ash in range from 9.24 % (CSR) to 14.67 % (CJR), level of calcium ranged from 0.76 % (CTR) to 1.37 % (CKR),content of phosphorus varied from 0.57 % (CCR) to 1.33 % (CTR). Antioxidant activity of ethanol extracts was in range from 6.84 % (CGR) to 11.65 % (CTR) and water extracts – from 0.76 % (CGR) to 2.52 % (CCR).
Conclusions. It can be concluded that underground part of plants of the genus Crambe is rich source of nutrients in the period of early spring. Some investigated species showed higher biological activity in comparison with ARR plants. Comparative analyze of phytochemical content of raw matter demonstrated that underground part of investigated plants can be competed with other food plants such as Armoracia rusticana by content of ascorbic acid, total content of sugars, organic acids, ash, macroelements etc. Total antioxidant activity of ethanol extracts of investigated plants demonstrated higher results than water extracts. |
| doi_str_mv | 10.5281/zenodo.2228725 |
| first_indexed | 2025-07-17T12:38:55Z |
| format | Article |
| fulltext |
3ISSN 16056574. Інтродукція рослин, 2018, № 2
UDC 582.683.2: 581.43: 581.192
O.M. VERGUN, D.B. RAKHMETOV, O.V. SHYMANSKA, V.V. FISHCHENKO
M.M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine
Ukraine, 01014 Kyiv, Timiryazevska str., 1
THE ACCUMULATION OF NUTRIENTS IN UNDERGROUND
PARTS OF PLANTS OF THE GENUS CRAMBE L. SPP.
Objective — to study the accumulation of biochemical compounds in the underground part of the plants of the genus Crambe L.
in the period of early spring in the conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine.
Material and methods. Plant material of this investigation — Crambe species (Brassicaceae Burnett): C. cordifolia Steven
(CCR), C. grandiflora DC. (CGR), C. juncea M.Bieb. (CJR), C. koktebelica (Junge) N. Busch (CKR), C. maritima L. (CMR),
C. steveniana Rupr. (CSR), C. tataria Sebeok (CTR). As control were selected plants of Armoracia rusticana P. Gaerth., B. Mey &
Scherb (ARR). All biochemical analyses were conducted using the underground part of plants in the period of early spring.
The determination of absolutely dry matter was done according to A.I. Yermakov, total content of sugars, ascorbic acid,
content of organic acids, tannins — according to V.P. Krishchenko, level of total ash — according to Z.M. Hrycajenko et al.,
concentration of calcium and phosphorus — according to H.N. Pochinok, antiradical activity of ethanol and water extracts —
according to W. BrandtWilliams et al.
Results. Obtained data showed that content of dry matter ranged from 16.00 % (CMR) to 22.48 % (CCR), total content of
sugar ranged from 11.03 % (CJR) to 46.48 % (CSR), content of ascorbic acid ranged from 192.85 mg% (CSR) to 483.45 mg%
(CTR), level of tannins in range from 1.64 % (CGR) to 5.12 % (CJR), content of organic acids ranged from 2.28 % (CSR)
to 3.64 % (CJR), content of ash in range from 9.24 % (CSR) to 14.67 % (CJR), level of calcium ranged from 0.76 % (CTR)
to 1.37 % (CKR),content of phosphorus varied from 0.57 % (CCR) to 1.33 % (CTR). Antioxidant activity of ethanol extracts
was in range from 6.84 % (CGR) to 11.65 % (CTR) and water extracts — from 0.76 % (CGR) to 2.52 % (CCR).
Conclusions. It can be concluded that underground part of plants of the genus Crambe is rich source of nutrients in the
period of early spring. Some investigated species showed higher biological activity in comparison with ARR plants. Comparative
analyze of phytochemical content of raw matter demonstrated that underground part of investigated plants can be competed with
other food plants such as Armoracia rusticana by content of ascorbic acid, total content of sugars, organic acids, ash, macroele
ments etc. Total antioxidant activity of ethanol extracts of investigated plants demonstrated higher results than water extracts.
Key words: Crambe, dry matter, ascorbic acid, tannins, ash, macroelements, antioxidant activity.
© O.M. VERGUN, D.B. RAKHMETOV,
O.V. SHYMANSKA, V.V. FISHCHENKO, 2018
10,000yearold traces of cultivation give evidence
that plants in the family Brassicaceae Burnett are
among the oldest cultivated plants known. These
plants grow under various climatic conditions and
accumulate different bioactive compounds that are
important for human health, food and animal feed
[10, 19, 25]. Cruciferous vegetables (e.g., Chinese
cabbage, broccoli, and mustard) are a major food
crop contributing to the diet of millions of people and
are of significant importance for agricultural eco no
mies worldwide. They have been independent ly do
mesticated for consumption, industrial products, and
medicine in Europe, the Middle East and Asia [16].
One of the most interesting plants of Brassica
ceae are the genus Crambe L. species, that indicate
the need for their widespread introduction and in
vestigation due to promising properties such as
food, decorative, medicinal etc. [21]. Nowadays
has carried out the study with Crambe seeds for
bio diesel production, mainly due to the high con
tent of oil in it [24]. As reported Wazilewski et al.
(2013) the crambe biodiesel is more stable than
the soybean biodiesel [26]. Raw material of these
plants has an antioxidant activity due to content of
phenolic compounds and flavonoids [15]. Plants
of the genus Crambe, as other species of Brassica
ceae, contain glucosinolates [20]. Also, Goncalves
et al. (2013) identified that the crambe (Crambe
abissinica Hochst) can be effective in the treat
4 ISSN 16056574. Інтродукція рослин, 2018, № 2
O.M. Vergun, D.B. Rakhmetov, O.V. Shymanska, V.V. Fishchenko
ment of wastewater containing toxic metals by be
ing a low cost option and a byproduct that requires
no previous treatment [29]. On the other hand,
some species of Crambe genus is threatened and
they require conservation measures [14, 16].
It was interesting to conduct biochemical com
parative analyze of these plants with wellknown
food and medical plants of Armoracia rusticana
P. Gaerth., B. Mey & Scherb (Horseradish) due to
similar pungent smell of roots. Previous data re
sulted, that horseradish plants contain compounds
that can act as natural antioxidants and antican
cer component [12, 23]. The main aim of this
study was to compare the accumulation of bio
chemical compounds in the underground part of
the genus Crambe L. species and plants of A. rusti
cana in the period of early spring in the conditions
of M.M. Gryshko National Botanical Garden of
the NAS of Ukraine.
Material and methods
Plant material was collected in M.M. Gryshko
National Botanical Garden of the NAS of Ukraine.
It was used to investigate plants of Crambe cordi
folia Steven (CCR), C. grandiflora DC. (CGR),
C. juncea M.Bieb. (CJR), C. koktebelica (Junge)
N. Busch (CKR), C. maritima L. (CMR), C. ste
veniana Rupr. (CSR), C. tataria Sebeok (CTR)
[3], as control — plants of Armoracia rusticana
P. Gaerth., B. Mey & Scherb (ARR).
All biochemical analyses were conducted using
the underground part of plants in the period of
early spring (the end of March) to screen accumu
lation of some nutrients.
The determination of absolutely dry matter was
done by drying to constant weight at 100—105 °С
according to A.I. Yermakov [5]. The total content
of sugars was investigated by Bertrand method in
water extracts. The concentration of ascorbic acid
(AA) of the acid extracts was determined by a
2.6dichlorophenolindophenol method that bas
ed on the reduction properties of AA. Total con
tent of organic acids was identified by titrimetric
method with phenolphthalein. Content of tannins
was determined by titrimetric method with reac
tion of indigo carmine discoloration. All these
analyses carried out according to V.P. Krischenko
[4]. The level of total ash was determined using the
method of combustion in muffleoven (SNOL
7.21100, Termolab) at 300—800 °С until the sam
ples turned into white ash to constant weight ac
cording to Z.M. Hrycajenko et al. [2]. The con
centration of calcium was determined by titration
method of acid extracts with Trilon B. Phosphorus
content in plants was identified in acid extracts us
ing molybdenum solution. Both these analyses
were done according to H.N. Pochinok [6].
Antioxidant capacity of the ethanolic and aque
ous extracts was determined according to W. Brand
Williams et al. (1995) against DPPH radical
(2,2di phenyl1picrylhydrazyl) [13]. This method
bas ed on reaction of radical discoloration. The pro
cedure of determination of optical density mea sured
with 2800 UV/VIS Spectrophotometer, UNICO
at wavelength 515 nm. Optical density of the solu
tion was measured after adding sample imme
diately and after 10 min of incubation in the dark.
Obtained results were calculated in percentage by
using formula: ((A
0
—A
10
)/A
0
)•100
(A
0
— absorbance of the control solution (con
taining only DPPH•); A
10
— absorbance in the
presence of the plant extract in DPPH• solution.
Mean values of three replicates and standard
deviations are given in Table 1, 2 and Fig. 1.
Experimental data were evaluated by using Ex
cel 2010.
Results and discussions
In the department of Cultural Flora of National
Botanical Garden of the NAS of Ukraine the bio
chemical research of different Brassicaceae repre
sentatives have conducted [1, 2, 28]. In this work
we compare the biochemical properties of Armo
racia rusticana (horseradish) and Crambe species.
Horseradish has been known since ancient
times as a folk medicinal herb and as a plant of
nutritional value and culinary interest. The tradi
tions to use horseradish plant for medicinal pur
pose are still applied in many countries. A. rusti
cana is a rich source of a number of bioactive
compounds such as glucosinolates, their break
down products, phenolic compounds [8, 30]. Al so,
some studies demonstrated antimicrobial, anti
fungal, an tiinflammatory and antioxidant ac tivity
5ISSN 16056574. Інтродукція рослин, 2018, № 2
The accumulation of nutrients in underground parts of plants of the genus Crambe L. spp.
of horseradish extracts [8, 9, 31]. Likewise, Cirim
bei et al. (2013) reported that horseradish root is
rich in vitamin C, B
1
, minerals (iron, potassium,
calcium, magnesium) [27]. According to Ciska et al.
(2017), glucoraphanin, glucoraphenin and napo
leiferin were noted for the first time in the tissues
of horseradish [18].
As shown in Table 1 dry matter of investigated
plants of the genus Crambe was in range from
16.00 % (CMR) to 22.48 % (CCR). Control sample
of ARR showed significant difference in the con
tent of dry matter if compare with the genus Cram
be species by 14.49—20.97 %. Content of dry mat
ter in underground part increased in the follow
ing order: CMR > CSR > CKR > CJR > CGR >
CTR > CCR > ARR. Total content of sugar was in
range from 11.03 % (CJR) to 46.48 % (CSR) and
plants of ARR showed accumulation of sugars
20.29 %. Underground part of ARR has accumu
lated total content of sugar on 26.19, 24.12, 13.47
and 1.72 % less than samples CSR, CMR, CTR
Table 1. The content of dry matter, vitamins, tannins and total content of sugar
in underground parts of plants of the genus Crambe L.
Sample Dry matter, % Total content of sugar, % Ascorbic acid, mg% Total content of tannins, %
CCR 22.48 ± 1.72 17.92 ± 1.80 352.98 ± 6.93 2.48 ± 0.29
CGR 21.34 ± 1.11 22.01 ± 1.14 220.87 ± 7.61 1.64 ± 0.31
CJR 19.71 ± 0.51 11.03 ± 0.54 305.08 ± 8.25 5.12 ± 0.52
CKR 19.54 ± 0.63 20.50 ± 1.84 207.85 ± 8.32 4.76 ± 0.34
CMR 16.00 ± 0.72 44.41 ± 2.04 416.34 ± 10.16 5.09 ± 0.42
CSR 16.52 ± 0.19 46.48 ± 0.45 192.85 ± 3.94 4.93 ± 0.41
CTR 22.05 ± 0.43 33.76 ± 0.48 483.45 ± 2.95 3.52 ± 0.31
ARR 36.97 ± 0.18 20.29 ± 0.67 316.51 ± 8.79 0.42 ± 0.18
and CGR respectively. Total content of sugars in
underground part increased in following order:
CJR > CCR > ARR > CKR > CGR > CTR >
CMR > CSR.
It should be noted that the content of ascorbic
acid was ranged from 192.85 mg% (CSR) to
483.45 mg% (CTR). Obtained result for ARR
plants was 316.51 mg%. Level of ascorbic acid in
samples CTR, CMR, CCR was more than in con
trol sample and difference was 166.94, 99.83 and
36.47 mg% respectively. Accumulation of ascorbic
acid was increased in following order: CSR > CKR
> CGR > CJR > ARR > CCR > CMR > CTR.
It was observed that level of tannins was in
range from 1.64 % (CGR) to 5.12 % (CJR) while
underground parts of ARR plants accumulated
the smallest amount of tannins (0.42 %) among
investigated plants. Level of tannins was identi
fied in following order from minimal to maxi
mum: ARR > CGR > CCR > CTR > CKR >
> CSR > CMR > CJR.
Table 2. The content of ash, macroelements and total content of organic acids
in underground parts of plants of the genus Crambe L., %
Sample Total content of organic acids Ash Calcium Phosphorus
CCR 3.32 ± 0.27 10.53 ± 0.54 1.09 ± 0.11 0.56 ± 0.01
CGR 3.61 ± 0.12 11.52 ± 1.10 0.89 ± 0.04 1.63 ± 0.02
CJR 3.64 ± 0.15 14.67 ± 1.31 1.18 ± 0.07 1.61 ± 0.07
CKR 3.19 ± 0.27 14.44 ± 1.68 1.37 ± 0.08 0.64 ± 0.03
CMR 4.00 ± 0.13 11.47 ± 0.83 1.36 ± 0.06 0.63 ± 0.04
CSR 2.28 ± 0.28 9.24 ± 0.59 0.92 ± 0.07 0.92 ± 0.02
CTR 3.59 ± 0.05 9.64 ± 0.03 0.76 ± 0.04 1.33 ± 0.04
ARR 2.50 ± 0.07 5.86 ± 0.37 0.74 ± 0.05 0.98 ± 0.02
6 ISSN 16056574. Інтродукція рослин, 2018, № 2
O.M. Vergun, D.B. Rakhmetov, O.V. Shymanska, V.V. Fishchenko
Total content of organic acids was ranged from
2.28 % (CSR) to 3.64 % (CJR) while ARR plants
had similar sign to CSR plants (2.50 %) (Table 2).
Samples of CTR, CMR, CKR, CJR, CGR, CCR
contained more organic acids than control sam
ple. Concentration of total organic acids increased
in investigated plants in following order: CSR >
ARR > CKR > CCR > CTR > CGR > CJR >
> CMR.
Content of ash was in the range from 9.24 %
(CSR) to 14.67 % (CJR) and increased in follow
ing order: ARR > CSR > CTR > CCR > CMR >
CGR > CKR > CJR. Level of calcium was ranged
from 0.76 % (CTR) to 1.37 % (CKR) and increased
in following order: ARR > CTR > CGR > CSR >
> CCR > CJR > CMR > CKR. Content of ash and
calcium in control plants was less relatively investi
gated plants of Crambe. Content of phosphorus va
ried from 0.56 % (CCR) to 1.33 % (CTR). Plants
of CGR, CJR and CTR contained more phospho
rus than plants of ARR. Concentration of phos
phorus in investigated plants increased in following
order: CCR > CMR > CKR > CSR > ARR > CTR >
> CJR > CGR.
We were interested to measure the antiradical
capacity of different extracts of investigated plants
(Fig. 1). It was chosen ethanol and aqueous ex
tracts for experiment only. In classic investigation
of antioxidant activity, it is customary to deter
mine inhibition of DPPH radical in the methanol
extracts also [13]. We proceeded from the fact that
plants A. rusticana are used as food plants with nu
merous properties for human healthy [12]. Pre
vious data on antioxidant capacity of extracts of
leaves and underground parts of horseradish show
ed strong activity [22]. The purpose of this study
was to compare biochemical capacity of plants of
Crambe and A. rusticana to recommend it for pos
sible use.
Figure 1 demonstrates antioxidant activity of
plant extracts of Crambe species and ARR. Gener
ally, ethanol extracts of investigated plants showed
inhibition of DPPH radical solution more than
water extracts. Antioxidant activity of ethanol ex
tracts was ranged of 6.84 % (CGR) to 11.65 %
(CTR) whereas ethanol extracts of ARR plants
showed inhibition by 13.75 %. Antioxidant capac
ity of water extracts for plants of the genus Crambe
was ranged from 0.76 % (CGR) to 2.52 % (CCR).
This result was less than for ARR control plants.
Minimal results were obtained for CGR plant ex
tracts, and inhibition index of ethanol extract was
Fig. 1. Antioxidant activity of ethanol and water extracts of plants of the genus Crambe L. (%):
Et — etanol extracts; W — water extracts
In
h
ib
it
io
n
,
%
14
12
10
8
6
4
2
0
CCR CGR CJR CKR CMR CSR CTR ARR
Et
W
7ISSN 16056574. Інтродукція рослин, 2018, № 2
The accumulation of nutrients in underground parts of plants of the genus Crambe L. spp.
9 times more than water extracts. The highest in
dex was marked for CTR plants (ethanol extracts)
and CCR plants (water extracts). Difference be
tween maximal indexes of different extracts was
in 4.6 times more for ethanol solutions. Accord
ing to Tomsone et al. (2012) the scavenging activ
ity of DPPH radical of root extracts of A. rusticana
ranged from 1.16 to 20.56 % depending from vari
ety [23]. Analyzed water extract of AAR showed
DPPH free radical scavenging activity of 46.22 %
as reported Istrati et al. (2013) [17].
Conclusions
This is the study providing results on biochemical
properties of underground parts of plants of the
genus Crambe L. Study showed that plant raw ma
terial of investigated plants are valuable source of
nutrients such as dry matter, vitamin C, tannins,
macroelements, ash, organic acid in early spring.
Comparative analysis between investigated plants
and plants of Armoracia rusticana demonstrated
that in underground part of A. rusticana content
of dry matter was the highest. Plants of Crambe
prevailed in content of ash and calcium. Summa
rizing obtained data, it can be noted that investi
gated plants even after winter period are able to
accumulate nutrients such as vitamins, macroele
ments, soluble sugars, tannins etc.
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8 ISSN 16056574. Інтродукція рослин, 2018, № 2
O.M. Vergun, D.B. Rakhmetov, O.V. Shymanska, V.V. Fishchenko
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�
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31. Total phenols and flavonoids content, antioxidant ca
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dx.doi.org/10.4236/fns.2015.61008
Recommended by R.V. Ivannikov
Received 29.01.2018
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http://dx.doi.org/10.4236/fns.2015.61008
Recommended by R.V. Ivannikov
Received 29.01.2018
О.М. Вергун, Д.Б. Рахметов,
О.В. Шиманська, В.В. Фіщенко
Національний ботанічний сад
імені М.М. Гришка НАН України,
Україна, м. Київ
НАКОПИЧЕННЯ ПОЖИВНИХ РЕЧОВИН
У ПІДЗЕМНІЙ ЧАСТИНІ РОСЛИН ВИДІВ
РОДУ CRAMBE L.
Мета — дослідити накопичення біохімічних речовин
у підземній частині видів роду Crambe L. у ранньовес
няний період в умовах Національного ботанічного
саду імені М.М. Гришка НАН України.
Матеріал та методи. Рослинний матеріал цього до
слідження — види роду Crambe L. (Brassicaceae Bur
nett.): C. cordifolia Steven (CCR), C. grandiflora DC.
(CGR), C. juncea M.Bieb. (CJR), C. koktebelica (Junge)
N. Busch (CKR), C. maritima L. (CMR), C. steveniana
Rupr. (CSR), C. tataria Sebeok (CTR). Рослини Armora
cia rusticana P. Gaerth., B. Mey & Scherb (ARR) слугува
ли контролем. Для всіх біохімічних аналізів викорис
товували підземну частину рослин у ранньовесняний
період. Абсолютно суху масу визначали за А.І. Єрма
ковим, загальний вміст цукрів, рівень аскорбінової
кислоти, органічних кислот, дубильних речовин — за
В.П. Крищенком, загальний вміст золи — за З.М. Гри
цаєнко, вміст кальцію та фосфору — за Х.Н. Почин
ком, антирадикальну активність етанольних та вод
них екстрактів — за W. BrandtWilliams et al.
Результати. Встановлено, що вміст сухої речовини
становив від 16,00 % (CMR) до 22,48 % (CCR), загаль
ний вміст цукрів — від 11,03 % (CJR) до 46,48 % (CSR),
вміст аскорбінової кислоти — від 192,85 мг% (CSR) до
483,45 мг% (CTR), дубильних речовин — від 1,64 %
(CGR) до 5,12 % (CJR), органічних кислот — від 2,28 %
(CSR) до 3,64 % (CJR), золи — від 9,24 % (CSR) до
14,67 % (CJR), кальцію — від 0,76 % (CTR) до 1,37 %
(CKR), фосфору — від 0,57 % (CCR) до 1,33 % (CTR).
Антиоксиданта активність етанольних екстрактів ста
новила від 6,84 % (CGR) до 11,65 % (CTR), водних —
від 0,76 % (CGR) до 2,52 % (CCR).
Висновки. Підземна частина рослин видів роду Cram
be L. — цінне джерело поживних речовин у ранньовес
няний період. Деякі з досліджуваних рослин виявили
вищу біологічну активність порівняно з рослинами
Armoracia rusticana. Порівняльний аналіз фі тохімічного
складу сировини показав, що досліджувані рослини
можуть конкурувати з іншими харчовими рослинами,
наприклад, з Armoracia rusticana, за загальним вмістом
аскорбінової кислоти, цукрів, ор га ніч них кислот, зо
ли, мікроелементів тощо. Загальна антиоксидантна
активність етанольних екстрактів досліджуваних рос
лин була вищою, ніж водних.
Ключові слова: Crambe, суха речовина, аскорбінова кис
лота, дубильні речовини, зола, макроелементи, анти
ок сидантна активність.
Е.Н. Вергун, Д.Б. Рахметов,
О.В. Шиманская, В.В. Фищенко
Национальный ботанический сад
имени Н.Н. Гришко НАН Украины,
Украина, г. Киев
НАКОПЛЕНИЕ ПИТАТЕЛЬНЫХ ВЕЩЕСТВ
В ПОДЗЕМНОЙ ЧАСТИ РАСТЕНИЙ ВИДОВ
РОДА CRAMBE L.
Цель — исследовать накопление биохимических ве
ществ в подземной части видов рода Crambe L. в ран
невесенний период в условиях Национального бота
нического сада имени Н.Н. Гришко НАН Украины.
Материал и методы. Растительный материал дан
ного исследования — виды рода Crambe (Brassicaceae
Burnett.): C. cordifolia Steven (CCR), C. grandiflora DC.
(CGR), C. juncea M.Bieb. (CJR), C. koktebelica (Junge)
N. Busch (CKR), C. maritima L. (CMR), C. steveniana
Rupr. (CSR), C. tataria Sebeok (CTR). Растения Armo
racia rusticana P. Gaerth., B. Mey & Scherb (ARR) слу
жили контролем. Для всех биохимических анализов
использовали подземную часть растений в ранневе
сенний период. Абсолютно сухую массу определяли
по А.И. Ермакову, общее содержание сахаров, уро
вень аскорбиновой кислоты, органических кислот,
дубильных веществ — по В.П. Крищенко, общее со
держание золы — по З.М. Грицаенко, содержание
кальция и фосфора — по Х.Н. Починку, антиради
11ISSN 16056574. Інтродукція рослин, 2018, № 2
The accumulation of nutrients in underground parts of plants of the genus Crambe L. spp.
кальную активность этанольных и водных экстрак
тов — по W. BrandtWilliams et al.
Результаты. Установлено, что содержание сухого ве
щества составило от 16,00 % (CMR) до 22,48 % (CCR),
общее содержание сахаров — от 11,03 % (CJR) до
46,48 % (CSR), содержание аскорбиновой кислоты —
от 192,85 мг% (CSR) до 483,45 мг% (CTR), дубильных
веществ — от 1,64 % (CGR) до 5,12 % (CJR), органиче
ских кислот — от 2,28 % (CSR) до 3,64 % (CJR), золы —
от 9,24 % (CSR) до 14,67 % (CJR), кальция — от 0,76 %
(CTR) до 1,37 % (CKR), фосфора — от 0,57 % (CCR) до
1,33 % (CTR). Антиоксидантая активность этанольных
екстрактов составляла от 6,84 % (CGR) до 11,65 %
(CTR), водных — от 0,76 % (CGR) до 2,52 % (CCR).
Выводы. Подземная часть растений видов рода
Crambe — ценный источник питательных веществ в
ран невесенний период. Некоторые из исследован
ных растений продемонстрировали более высокую
био логическую активность по сравнению с рас
те ниями Armoracia rusticana. Сравнительный анализ
фитохимического состава сырья показал, что иссле
дованные растения могут конкурировать с другими
пищевыми растениями, например, с Armoracia rusti
cana, по общему содержанию сахара, аскорбиновой
кислоты, органических кислот, золы, макроелемен
тов и т.д. Общая антиоксидантная активность эта
нольных экстрактов исследуемых растений была
вы ше, чем водных.
Ключевые слова: Crambe, сухое вещество, аскорби
новая кислота, дубильные вещества, зола, макроеле
менты, антиоксидантная активность.
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| id | oai:ojs2.plantintroduction.org:article-19 |
| institution | Plant Introduction |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2025-07-17T12:38:55Z |
| publishDate | 2018 |
| publisher | M.M. Gryshko National Botanical Garden of the NAS of Ukraine |
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| resource_txt_mv | wwwplantintroductionorg/4d/e31d82317ff003d319e2d0b43b818c4d.pdf |
| spelling | oai:ojs2.plantintroduction.org:article-192019-11-11T08:15:12Z The accumulation of nutrients in underground parts of plants of the genus Crambe L. spp. Накопичення поживних речовин у підземній частині рослин видів роду Crambe L. Vergun, O.M. Rakhmetov, D.B. Shymanska, O.V. Fishchenko, V.V. Objective – to study the accumulation of biochemical compounds in the underground part of the plants of the genus Crambe L. in the period of early spring in the conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. Plant material of this investigation – Crambe species (Brassicaceae Burnett): C. cordifolia Steven (CCR), C. grandiflora DC. (CGR), C. juncea M.Bieb. (CJR), C. koktebelica (Junge) N. Busch (CKR), C. maritima L. (CMR), C. steveniana Rupr. (CSR), C. tataria Sebeok (CTR). As control were selected plants of Armoracia rusticana P. Gaerth., B. Mey et Scherb (ARR). All biochemical analyses were conducted using the underground part of plants in the period of early spring. The determination of absolutely dry matter was done according to A.I. Yermakov, total content of sugars, ascorbic acid, content of organic acids, tannins – according to V.P. Krishchenko, level of total ash – according to Z.M. Hrycajenko et al., concentration of calcium and phosphorus – according to H.N. Pochinok, antiradical activity of ethanol and water extracts – according to W. Brandt-Williams et al. Results. Obtained data showed that content of dry matter ranged from 16.00 % (CMR) to 22.48 % (CCR), total content of sugar ranged from 11.03 % (CJR) to 46.48 % (CSR), content of ascorbic acid ranged from 192.85 mg% (CSR) to 483.45 mg% (CTR), level of tannins in range from 1.64 % (CGR) to 5.12 % (CJR), content of organic acids ranged from 2.28 % (CSR) to 3.64 % (CJR), content of ash in range from 9.24 % (CSR) to 14.67 % (CJR), level of calcium ranged from 0.76 % (CTR) to 1.37 % (CKR),content of phosphorus varied from 0.57 % (CCR) to 1.33 % (CTR). Antioxidant activity of ethanol extracts was in range from 6.84 % (CGR) to 11.65 % (CTR) and water extracts – from 0.76 % (CGR) to 2.52 % (CCR). Conclusions. It can be concluded that underground part of plants of the genus Crambe is rich source of nutrients in the period of early spring. Some investigated species showed higher biological activity in comparison with ARR plants. Comparative analyze of phytochemical content of raw matter demonstrated that underground part of investigated plants can be competed with other food plants such as Armoracia rusticana by content of ascorbic acid, total content of sugars, organic acids, ash, macroelements etc. Total antioxidant activity of ethanol extracts of investigated plants demonstrated higher results than water extracts. Мета – дослідити накопичення біохімічних речовин у підземній частині видів роду Crambe L. у ранньовесняний період в умовах Національного ботанічного саду імені М.М. Гришка НАН України. Матеріал та методи. Рослинний матеріал цього дослідження – види роду Crambe L. (Brassicaceae Burnett.): C. cordifolia Steven (CCR), C. grandiflora DC. (CGR), C. juncea M.Bieb. (CJR), C. koktebelica (Junge) N. Busch (CKR), C. maritima L. (CMR), C. steveniana Rupr. (CSR), C. tataria Sebeok (CTR). Рослини Armoracia rusticana P. Gaerth., B. Mey & Scherb (ARR) слугували контролем. Для всіх біохімічних аналізів використовували підземну частину рослин у ранньовесняний період. Абсолютно суху масу визначали за А.І. Єрмаковим, загальний вміст цукрів, рівень аскорбінової кислоти, органічних кислот, дубильних речовин – за В.П. Крищенком, загальний вміст золи – за З.М. Грицаєнко, вміст кальцію та фосфору – за Х.Н. Починком, антирадикальну активність етанольних та водних екстрактів – за W Brandt-Williams et al. Результати. Встановлено, що вміст сухої речовини становив від 16,00 % (CMR) до 22,48 % (CCR), загальний вміст цукрів – від 11,03 % (CJR) до 46,48 % (CSR), вміст аскорбінової кислоти – від 192,85 мг% (CSR) до 483,45 мг% (CTR), дубильних речовин – від 1,64 % (CGR) до 5,12 % (CJR), органічних кислот – від 2,28 % (CSR) до 3,64 % (CJR), золи – від 9,24 % (CSR) до 14,67 % (CJR), кальцію – від 0,76 % (CTR) до 1,37 % (CKR), фосфору – від 0,57 % (CCR) до 1,33 % (CTR). Антиоксиданта активність етанольних екстрактів становила від 6,84 % (CGR) до 11,65 % (CTR), водних – від 0,76 % (CGR) до 2,52 % (CCR). Висновки. Підземна частина рослин видів роду Crambe L. – цінне джерело поживних речовин у ранньовесняний період. Деякі з досліджуваних рослин виявили вищу біологічну активність порівняно з рослинами Armoracia rusticana. Порівняльний аналіз фітохімічного складу сировини показав, що досліджувані рослини можуть конкурувати з іншими харчовими рослинами, наприклад, з Armoracia rusticana, за загальним вмістом аскорбінової кислоти, цукрів, органічних кислот, золи, мікроелементів тощо. Загальна антиоксидантна активність етанольних екстрактів досліджуваних рослин була вищою, ніж водних. M.M. Gryshko National Botanical Garden of the NAS of Ukraine 2018-05-01 Article Article application/pdf https://www.plantintroduction.org/index.php/pi/article/view/19 10.5281/zenodo.2228725 Plant Introduction; Vol 78 (2018); 3-11 Інтродукція Рослин; Том 78 (2018); 3-11 2663-290X 1605-6574 10.5281/zenodo.3377684 en https://www.plantintroduction.org/index.php/pi/article/view/19/14 Copyright (c) 2018 The Author(s) http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Vergun, O.M. Rakhmetov, D.B. Shymanska, O.V. Fishchenko, V.V. Накопичення поживних речовин у підземній частині рослин видів роду Crambe L. |
| title | Накопичення поживних речовин у підземній частині рослин видів роду Crambe L. |
| title_alt | The accumulation of nutrients in underground parts of plants of the genus Crambe L. spp. |
| title_full | Накопичення поживних речовин у підземній частині рослин видів роду Crambe L. |
| title_fullStr | Накопичення поживних речовин у підземній частині рослин видів роду Crambe L. |
| title_full_unstemmed | Накопичення поживних речовин у підземній частині рослин видів роду Crambe L. |
| title_short | Накопичення поживних речовин у підземній частині рослин видів роду Crambe L. |
| title_sort | накопичення поживних речовин у підземній частині рослин видів роду crambe l. |
| url | https://www.plantintroduction.org/index.php/pi/article/view/19 |
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