Антирадикальна активність рослинних екстрактів Galega officinalis L. та G. orientalis Lam.
Objective – to estimate the antiradical scavenging ability of extracts of plants of Galega officinalis L. and G. orientalis Lam., depending on phase of growing in the conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG). Material and methods. Plant material of this invest...
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M.M. Gryshko National Botanical Garden of the NAS of Ukraine
2018
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| author | Shymanska, O.V. Vergun, O.M. Rakhmetov, D.B. Brindza, J. |
| author_facet | Shymanska, O.V. Vergun, O.M. Rakhmetov, D.B. Brindza, J. |
| author_sort | Shymanska, O.V. |
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| datestamp_date | 2019-11-11T08:15:12Z |
| description | Objective – to estimate the antiradical scavenging ability of extracts of plants of Galega officinalis L. and G. orientalis Lam., depending on phase of growing in the conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG).
Material and methods. Plant material of this investigation was two species of Galega L. (G. officinalis and G. orientalis) collected from experimental collection of Cultural Flora Department of NBG: GOFSV (G. officinalis, spring vegetation), GOFB (G. officinalis, budding stage), GOFF (G. officinalis, flowering stage), GOFSR (G. officinalis, seed ripening stage), GORSV (G. orientalis, spring vegetation), GORB (G. orientalis, budding stage), GORF (G. orientalis, flowering stage), GORSR (G. orientalis, seed ripening stage). The antiradical activity of methanol, ethanol and aqueous extracts, based on the discoloration reaction on the solution of DPPH (2,2-diphenyl-1-picrylhydrazyl free radical), was determined by spectrophotometric method according to Brandt-Williams et al. Biochemical preparation and analyze was carried out in the Institute of Biodiversity Conservation and Biosafety, the Slovak University of Agriculture in Nitra (Slovak Republic) and NBG. The total content of tannins was determined by titrimetric method according to Krischenko (water extracts with indigo carmine were titrated by permanganate solution).
Results. We determined that methanol extracts of Galega officinalis plants had antiradical activity in range from 19.39 % (GOFB) to 95.18 % (GOFSR), ethanol extracts – from 11.24 % (GOFB) to 92.87 % (GOFF), and water extracts – from 28.64 % (GOFSR) to 74.63 % (GOFF). Methanol extracts of Galega orientalis plants had antiradical activity from 20.20 % (GORSR) to 91.72 % (GORB), ethanol extracts – from 11.74 % (GORSR) to 84.74 % (GORF), and water extracts – from 22.90 % (GORSV) to 77.72 % (GORF). The total content of tannins for G. officinalis was in range of 1.22 to 4.17 % and for G. orientalis – from 1.55 to 4.42 % during vegetation.
Conclusions. Plant raw material of two Galega L. species is potential source of antioxidants. During vegetation antiradical activity of plant extracts of Galega officinalis exhibited 11.24–95.18 % and Galega orientalis – 11.74–91.72 % depending on extract and phase of growing in conditions of NBG. Generative organs such as flowers and fruits had less content of tannins than vegetative. |
| doi_str_mv | 10.5281/zenodo.2229075 |
| first_indexed | 2025-07-17T12:38:57Z |
| format | Article |
| fulltext |
12 ISSN 16056574. Інтродукція рослин, 2018, № 2
UDC 582.736.3: 633.37: 581.192
O.V. SHYMANSKA 1, O.M. VERGUN 1, D.B. RAKHMETOV 1, J. BRINDZA 2
1 M.M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine
Ukraine, 01014 Kyiv, Timiryazevska str., 1
2 Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources,
Institute of Biodiversity Conservation and Biosafety
Slovakia, 94976 Nitra, Trieda A. Hlinku str., 2
ANTIRADICAL ACTIVITY OF PLANT EXTRACTS
OF GALEGA OFFICINALIS L. AND G. ORIENTALIS LAM.
Objective — to estimate the antiradical scavenging ability of extracts of plants of Galega officinalis L. and G. orientalis Lam.,
depending on phase of growing in the conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG).
Material and methods. Plant material of this investigation was two species of Galega L. (G. officinalis and G. orientalis)
collected from experimental collection of Cultural Flora Department of NBG: GOFSV (G. officinalis, spring vegetation), GOFB
(G. officinalis, budding stage), GOFF (G. officinalis, flowering stage), GOFSR (G. officinalis, seed ripening stage), GORSV
(G. orientalis, spring vegetation), GORB (G. orientalis, budding stage), GORF (G. orientalis, flowering stage), GORSR
(G. orientalis, seed ripening stage). The antiradical activity of methanol, ethanol and aqueous extracts, based on the discoloration
reaction on the solution of DPPH (2,2diphenyl1picrylhydrazyl free radical), was determined by spectrophotometric method
according to BrandtWilliams et al. Biochemical preparation and analyze was carried out in the Institute of Biodiversity
Conservation and Biosafety, the Slovak University of Agriculture in Nitra (Slovak Republic) and NBG. The total content
of tannins was determined by titrimetric method according to Krischenko (water extracts with indigo carmine were titrated
by permanganate solution).
Results. We determined that methanol extracts of Galega officinalis plants had antiradical activity in range from 19.39 %
(GOFB) to 95.18 % (GOFSR), ethanol extracts — from 11.24 % (GOFB) to 92.87 % (GOFF), and water extracts — from
28.64 % (GOFSR) to 74.63 % (GOFF). Methanol extracts of Galega orientalis plants had antiradical activity from 20.20 %
(GORSR) to 91.72 % (GORB), ethanol extracts — from 11.74 % (GORSR) to 84.74 % (GORF), and water extracts — from
22.90 % (GORSV) to 77.72 % (GORF). The total content of tannins for G. officinalis was in range of 1.22 to 4.17 % and for
G. orientalis — from 1.55 to 4.42 % during vegetation.
Conclusions. Plant raw material of two Galega L. species is potential source of antioxidants. During vegetation antiradical activity
of plant extracts of Galega officinalis exhibited 11.24—95.18 % and Galega orientalis — 11.74—91.72 % depending on extract and
phase of growing in conditions of NBG. Generative organs such as flowers and fruits had less content of tannins than vegetative.
Key words: Galega, antioxidant activity, 2,2diphenyl1picrylhydrazyl (DPPH), tannins.
© O.V. SHYMANSKA, O.M. VERGUN,
D.B. RAKHMETOV, J. BRINDZA, 2018
Plants have played a major role in the introduc
tion of new antioxidant and therapeutic agents
[24]. Species of Galega L. now are widely recom
mended plants administered for production of
firstline antidiabetic drugs, which is safe and ef
ficient in the treatment of diabetes and usually
does not induce hypoglycemia such as Metformin
[1, 8, 10, 15, 27, 28]. As reported Shojaee et al.
(2015) G. officinalis L. extracts possess com
pounds with hypoglycemia and weightreducing
potential [12, 18]. Karakas et al. (2012) resulted
that this plant has been used for treatment of the
plague, malignant fevers, and parasitic infec
tion [17]. According to Leporatti and Ivancheva
(2003), aerial parts of G. officinalis use in tradi
tional medicine of Bulgaria and Italy for treat
ment of hypoglycemia and for increasing milk
secretion [19].
Also, G. officinalis is used as an ornamental
plant and occurs as a weed [29]. These plants con
tain vasicine and a poisonous alkaloid known as
galegin. Plant extracts were tested against gram
positive and gramnegative bacteria and the anti
bacterial effect was shown [17]. Also, an antimi
crobial activity of G. officinalis was described and
found that extracts against bacteria was more ef
fective than against fungus [21].
13ISSN 16056574. Інтродукція рослин, 2018, № 2
Antiradical activity of plant extracts of Galega officinalis L. and G. orientalis Lam.
Kiselova et al. (2006) reported, that G. officinalis
plant extracts have strong correlation between con
tent of polyphenols and antioxidant activity [11].
Galega orientalis Lam. is very persistent with a
high yielding ability that makes focus on study of
this plant as plant raw material for energy and fod
der production [7, 13].
The antioxidant properties of cultivated plants
are usually well recognized. There is, however,
little data about antioxidant activity of Galega
species. Therefore further studies must be car
ried out.
Material and methods
Plant material was collected from experimental collec
tion of Cultural Flora Department of M.M. Grysh
ko National Botanical Garden of the NAS of Uk
raine (NBG). It was selected plants of two species
of Galega — G. officinalis and G. orientalis. In this
report has used abbreviation: GOFSV (G. offi
cinalis, spring vegetation), GOFB (G. officinalis,
budding stage), GOFF (G. officinalis, flower
ing stage), GOFSR (G. officinalis, seed ripening
stage), GORSV (G. orientalis, spring vegetation),
GORB (G. orientalis, budding stage), GORF
(G. orientalis, flowering stage), GORSR (G. orien
talis, seed ripening stage).
Preparation of plant raw material and determi
nation of tannins content was done in the labora
tory of Cultural Flora Department of NBG. De
termination of antiradical activity of plant extracts
were conducted in the Institute of Biodiversity
Conservation and Biosafety, the Slovak University
of Agriculture in Nitra (Slovak Republic). To deter
mine antioxidant activity of extracts was investi
gated dried aboveground part of plants. Antiradical
activity of the methanolic, ethanolic and aqueous
extracts was carried out according to BrandWil
liams et al. (1995) against DPPH radical (2.2di
phenyl1picrylhydrazyl) [9]. This me thod based
on the reaction of radical discolouration (colour of
the radical solution is purple). The procedure of
determination of optical density measured with spec
trophotometer Genesis20 at wavelength 515 nm.
Dry mass (1 g) of investigated plants mixed with 25 ml
of solvent. Extraction was carried out with metha
nol and water during 12 hours with constant stir
ring on a shaker. 0.1 ml of antioxidant solution
was added to 3.9 ml of methanol DPPH·solution
(25 mg of radical per 100 ml of methanol with fur
ther dilution). The optical density of the solution
was measured after adding sample immediately
and after 10 min of incubation in the dark. Obtained
results were calculated in percentage by using
the formula
(A
0
—A
10
):A
0
·100
(A
0
— absorbance of the control solution (contain
ing only DPPH•); A
10
— absorbance in the pres
ence of the plant extract in DPPH• solution).
The total content of tannins was determined by
titrimetric method according to Krischenko (1983)
[3]. The samples (5 g of fresh plant raw material)
were mixed with distilled water (volume of flask
100 ml) and boiled 2 hours at the 70 °C. The su
pernatant was analyzed by adding 10 ml of the
sample and 25 ml of indigo carmine to a 1 liter
flask and then adding 750 ml of water with follow
ing titration against the permanganate solution
(0.1 N).
Data presented as the mean ± standard devia
tion for triplicate determinations and given in
Tab le 1—3. Experimental data were evaluated us
ing Excel 2010.
Results and discussions
Medicinal plants synthesize antioxidant com
pounds as secondary products [1, 4, 6, 22]. Plan
raw material of investigated plants is rich source of
biologically active compounds such as ascorbic
acid, lipids, carotene, tannins that makes above
ground part of Galega species potentially rich plant
raw material with high level of antioxidant activity.
Our previous biochemical investigations showed
high content of ascorbic acid in the aboveground
part of G. officinalis (595.12 mg%), G. orientalis
(436.70 mg%). Carotene content was 2.07 mg%
and 1.49 mg% respectively [2]. Also, Shymanska
et al. (2017) found that content of carotenoids for
G. officinalis was from 0.57 to 0.88 mg·g1 and for
G. orientalis — from 0.31 to 1.05 mg·g1 (per fresh
mass) [26].
There is great number of methods for determi
nation of antioxidant capacity of plant raw mate
rial based on different principles. One of the most
14 ISSN 16056574. Інтродукція рослин, 2018, № 2
O.V. Shymanska, O.M. Vergun, D.B. Rakhmetov, J. Brindza
widelyused procedures for measurements of anti
oxidant capacity is DPPH. This method is rapid,
simple, accurate and inexpensive assay for meas
uring the ability of different compounds to act as
free radical scavengers or hydrogen donor [20].
Table 1. Antiradical activity of plant extracts of Galega officinalis L. depending on phase of growing and parts of plants, %
Sample Part of plant Methanol extract Ethanol extract Water extract
GOFSV All aboveground part 52.04 ± 2.19 23.62 ± 1.44 38.57 ± 1.65
GOFB Buds 85.50 ± 0.52 40.05 ± 1.18 71.06 ± 3.46
GOFB Leaves 70.43 ± 3.36 42.55 ± 0.98 66.79 ± 0.72
GOFB Stems 19.39 ± 1.13 11.24 ± 0.80 29.31 ± 2.94
GOFF Flowers 92.26 ± 0.68 92.87 ± 1.03 74.63 ± 0.83
GOFF Leaves 88.25 ± 2.83 77.29 ± 1.36 55.33 ± 3.48
GOFF Stems 19.54 ± 1.12 20.83 ± 0.50 30.76 ± 0.73
GOFSR Fruits 95.18 ± 1.54 53.08 ± 2.82 43.80 ± 2.26
GOFSR Leaves 79.72 ± 3.59 62.03 ± 3.01 54.63 ± 0.67
GOFSR Stems 26.03 ± 1.05 18.80 ± 1.33 28.61 ± 1.30
Antiradical activity of extracts exhibit the accu
mulation of a group of compounds that react with
DPPH radical and change the color of radical so
lution (from purple to yellow or green depending
on investigated extracts ). Results of the antiradical
Table 2. Antiradical activity of plant extracts of Galega orientalis Lam. depending on phase of growing and parts of plants, %
Sample Part of plant Methanol extract Ethanol extract Water extract
GORSV All aboveground part 53.96 ± 1.66 19.07 ± 2.14 22.90 ± 2.44
GORB Buds 91.72 ± 0.21 58.44 ± 7.26 50.38 ± 1.37
GORB Leaves 85.41 ± 2.06 43.33 ± 1.55 50.22 ± 2.87
GORB Stems 23.69 ± 1.92 12.59 ± 1.25 31.00 ± 1.52
GORF Flowers 90.88 ± 0.56 84.78 ± 2.66 77.72 ± 4.58
GORF Leaves 87.72 ± 2.08 37.74 ± 3.15 48.33 ± 3.06
GORF Stems 23.77 ± 1.81 15.59 ± 0.82 28.40 ± 1.81
GORSR Fruits 90.60 ± 2.63 20.66 ± 1.43 59.72 ± 2.43
GORSR Leaves 41.78 ± 2.95 22.93 ± 2.42 44.65 ± 0.86
GORSR Stems 20.20 ± 0.90 11.74 ± 0.62 24.33 ± 1.56
Table 3. Total content of tannins in water extracts of Galega L. species depending on phase of growing
and parts of plants, %
Sample Part of plant Tannins Sample Part of plant Tannins
GOFSV All aboveground part 2.56 ± 0.29 GORSV All aboveground part 1.77 ± 0.21
GOFB Buds 2.89 ± 0.04 GORB Buds 2.44 ± 0.13
GOFB Leaves 3.23 ± 0.15 GORB Leaves 3.94 ± 0.22
GOFB Stems 1.54 ± 0.14 GORB Stems 1.87 ± 0.11
GOFF Flowers 2.06 ± 0.11 GORF Flowers 1.55 ± 0.06
GOFF Leaves 4.17 ± 0.23 GORF Leaves 3.56 ± 0.18
GOFF Stems 1.78 ± 0.13 GORF Stems 2.23 ± 0.16
GOFSR Fruits 1.22 ± 0.09 GORSR Fruits 1.94 ± 0.07
GOFSR Leaves 4.89 ± 0.32 GORSR Leaves 4.42 ± 0.15
GOFSR Stems 2.23 ± 0.11 GORSR Stems 1.73 ± 0.05
15ISSN 16056574. Інтродукція рослин, 2018, № 2
Antiradical activity of plant extracts of Galega officinalis L. and G. orientalis Lam.
activity of plant extracts of two species of Gale ga
are reported in Table 1 and 2.
Previous data, obtained by Tusevski et al.
(2014), showed a high antioxidant potential of
G. officinale plant extracts due to content of phe
nolic compounds [22].
Methanol extracts of G. officinale plants exhib
ited antiradical scavenging from 19.39 % (GOFB,
stems) to 95.18 % (GOFSR, fruits) during veg
etation (Table 1). Ethanol extracts showed anti
radical activity from 11.24 % (GOFB, stems) to
92.87 % (GOFF, flowers). Antiradical activity of
water extracts was of 28.61 % (GOFSR, stems)
to 74.63 % (GOFF, flowers).
Methanol extracts of G. orientalis plants exhib
ited antiradical scavenging from 20.20 % (GOR
SR, stems) to 91.72 % (GORB, buds) during veg
etation (Table 2). Ethanol extracts showed anti
radical activity from 11.74 % (GORSR, stems) to
84.78 % (GORF, flowers). Antiradical activity of
water extracts was from 22.90 % (GORSV, all
plant) to 77.72 % (GORF, flowers).
Also, we determined the content of total tan
nins in the water extracts of investigated plants
(Table 3). Tannins are naturally occurring poly
phenols produced by plants via secondary meta
bolic processes. Their ability to bind proteins, pig
ments, and complex metallic ions, together with
their flavouring effect are the basis for their exten
sive use as additives in the food industry [25]. Tan
nins don’t function solely as primary antioxidants
but as secondary antioxidants [5]. According to
last study in this branch, it was found correlation
between antioxidant activity and compounds of
phenolic nature [23].
As presented in Table 3, the total content of
tannins noticed in the leaves depending on phase
of plant growing. Generative organs such as flow
ers and fruits had less content of tannins than
vege tative: G. officinalis — from 1.22 to 2.89 %
(generative), from 1.54 to 4.89 (vegetative); G. ori
entalis — from 1.55 to 2.44 % (generative), from
1.73 to 4.42 (vegetative).
Conclusions
Thus, this study was shown that plant raw material
of the genus Galega is potential source of antioxi
dants. The antiradical activity of plant extracts
of G. officinalis was minimal in stems in the period
of budding (11.24 %) and maximal — in fruits in
the fruitage period (95.18 %) depending on extract.
The same sign for G. orientalis plants was minimal
in stem in the seed ripening period (11.74 %) and
maximal — in buds in the budding period (91.72 %)
depending on extract. The high antiradical activity
identified in methanol and ethanol extracts (up to
90 %). The content of tannins for G. officinalis was
in range of 1.22 to 4.17 % and for G. orientalis —
from 1.55 to 4.42 % during vegetation.
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logy. — 2001. — Vol. 49 (3). — P. 240—247. [Електрон
ний ресурс]. — Режим доступу: https://doi.org/10.3
109/13880209.2010.201083
24. RafieianKopaei M. Plants antioxidants: from labo
ratory to clinic / M. RafieianKopaei, A. Baradaran,
M. Ra fieian // Journal of Nephropathology. — 2013. —
Vol. 2, N 2. — P. 152—153. [Електронний ресурс]. —
Режим доступу: http://dx.doi.org/10.12860/JNP.
2013.26
25. Rapid screening method to assess tannin antioxidant
activity in foodgrade botanical extract / A. Schwert
ner Palma, A. Ricci, G.P. Parpinello, A. Versari // Ital
ian Journal of Food Sciences. — 2017. — Vol. 29. —
P. 559—564.
26. The content of photosynthetic pigments in the leaves of
Galega officinalis L. and Galega orientalis Lam. culti
vars / O. Shymanska, O. Vergun, D. Rakhmetov, V. Fi sh
chenko // Agrobiodiversity for improving nutrition,
health and life quality. — 2017. — Vol. 1. — P. 398—403.
[Електронний ресурс]. — Режим доступу: http://dx.
doi.org/10.15414/agrobiodiversity.2017.25858246.
398403
27. Traditional medicinal plants curing diabetes: a promise
for today and tomorrow / S. Kumar, M. Saini, V. Kumar
[et al.] // Asian Journal of Traditional Medicines. —
2012. — Vol. 7, N 4. — P. 178—188.
28. Umashanker M. Traditional Indian herbal medicine
used as antipyretic, antiulcer, antidiabetic and anti
cancer: a review / M. Umashanker, S. Shruti // Inter
national Journal of Research in Pharmacy and Chem
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29. Varis E. Goat’s rue (Galega orientalis Lam.) a potential
pasture legume for temperate conditions / E. Varis //
Journal of Agricultural Sciences of Finland. — 1986. —
Vol. 58. — P. 83—101.
Recommended by N.A. Pavliuchenko
Received 01.02.2018
17ISSN 16056574. Інтродукція рослин, 2018, № 2
Antiradical activity of plant extracts of Galega officinalis L. and G. orientalis Lam.
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18 ISSN 16056574. Інтродукція рослин, 2018, № 2
O.V. Shymanska, O.M. Vergun, D.B. Rakhmetov, J. Brindza
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Recommended by N.A. Pavliuchenko
Received 01.02.2018
О.В. Шиманська 1, Д.Б. Рахметов 1,
О.М. Вергун 1, Я. Бріндза 2
1 Національний ботанічний сад імені М.М. Гришка
НАН України, Україна, м. Київ
2 Словацький аграрний університет в Нітрі,
факультет Агробіології та харчових ресурсів,
Інститут збереження біорізноманіття
та біологічної безпеки, Словацька Республіка, Нітра
АНТИРАДИКАЛЬНА АКТИВНІСТЬ
РОСЛИННИХ ЕКСТРАКТІВ GALEGA
OFFICINALIS L. ТА G. ORIENTALIS LAM.
Мета — дослідити антирадикальну дію екстрактів
рослин Galega officinalis L. та G. orientalis Lam. залежно
від фази розвитку в умовах Національного ботанічно
го саду імені М.М. Гришка НАН України (НБС).
Матеріал та методи. Рослинний матеріал досліджен
ня — два види роду Galega L. (G. officinalis та G. orientalis),
зібрані з експериментальної колекції відділу культурної
флори НБС: GOFSV (G. officinalis, весняне відростання),
GOFB (G. officinalis, бутонізація), GOFF (G. officinalis,
цвітіння), GOFSR (G. officinalis, дозрівання насіння),
GORSV (G. orientalis, весняне відростання), GORB
(G. orientalis, бутонізація), GORF (G. orientalis, цвітіння),
GORSR (G. orientalis, дозрівання насіння). Антиради
кальну активність метанольних, етанольних та водних
екстрактів, яка ґрунтується на реакції знебарвлення
розчину ДФПГ (2,2дифеніл1пікрилгідразил вільний
радикал), визначали спектрофотометричним методом
за W. Brandt Williams et al. Біохімічний аналіз прове
дено в Інституті збереження біорізноманіття та біоло
гічної безпеки Словацького аграрного університету в
Нітрі (Слова цька Республіка) та НБС. Загальний вміст
дубильних речовин визначено методом титрування за
В.П. Крищенком (водні екстракти з індигокарміном
титрували розчином перманганату).
Результати. Визначено, що метанольні екстракти
рос лин Galega officinalis виявляли антирадикальну ак
тивність від 19,39 % (GOFB) до 95,18 % (GOFSR), ета
нольні екстракти — від 11,24 % (GOFB) до 92,87 %
(GOFF), водні екстракти — від 28,64 % (GOFSR) до
74,63 % (GOFF), метанольні екстракти рослин Galega
orientalis — від 20,20 % (GORSR) до 91,72 % (GORB),
етанольні екстракти — від 11,74 % (GORSR) до 84,74 %
(GORF), водні екстракти — від 22,90 % (GORSV) до
77,72 % (GORF). Загальний вміст дубильних речовин
для G. officinalis становив від 1,22 до 4,17 %, для G. orien
talis — від 1,55 до 4,42 % протягом вегетації.
Висновки. Рослинна сировина двох видів роду Ga
lega — потенційне джерело антиоксидантів. Протягом
вегетації рослинні екстракти Galega officinalis виявля
ли антирадикальну активність від 11,24 до 95,18 %,
Galega orientalis — від 11,74 до 91,72 % залежно від екс
тракту та фази розвитку в умовах НБС. Генеративні
органи характеризувалися нижчим вмістом дубиль
них речовин, ніж вегетативні.
Ключові слова: Galega, антиоксидантна активність, 2,2ди
феніл1пікрилгідразил (ДФПГ), дубильні речовини.
О.В. Шиманская1, Д.Б. Рахметов 1,
Е.Н. Вергун 1, Я. Бриндза 2
1 Национальный ботанический сад имени Н.Н. Гришко
НАН Украины, Украина, г. Киев
2 Словацкий аграрный университет в Нитре,
факультет Агробиологии и пищевых ресурсов,
Институт сохранения биоразнообразия
и биологической безопасности,
Словацкая Республика, Нитра
АНТИРАДИКАЛЬНАЯ АКТИВНОСТЬ
РАСТИТЕЛЬНЫХ ЭКСТРАКТОВ GALEGA
OFFICINALIS L. И G. ORIENTALIS LAM.
Цель — исследовать антирадикальное действие экс
трактов растений Galega officinalis L. и G. orientalis Lam.
в зависимости от фазы развития в условиях Нацио
19ISSN 16056574. Інтродукція рослин, 2018, № 2
Antiradical activity of plant extracts of Galega officinalis L. and G. orientalis Lam.
нального ботанического сада имени Н.Н. Гришко
НАН Украины (НБС).
Материал и методы. Растительный материал ис
следования — два вида рода Galega L. (G. officinalis и
G. orientalis), собранные из экспериментальной кол
лекции отдела культурной флоры НБС: GOFSV
(G. officinalis, весеннее отрастание), GOFB (G. offici
nalis, бутонизация), GOFF (G. officinalis, цветение),
GOFSR (G. officinalis, созревание семян), GORSV
(G. orientalis, весеннее отрастание), GORB (G. orien
talis, бутонизация), GORF (G. orientalis, цветение),
GORSR (G. orientalis, созревание семян). Антиради
кальную активность метанольных, этанольных и вод
ных экстрактов, основанную на реакции обесцве
чивания раствора ДФПГ (2,2дифенил1пик рил
гид разил свободный радикал), определяли спектро
фо тометрическим методом по W. BrandtWilliams
et al. Биохимический анализ проведен в Институте
сохранения биоразнообразия и биологической бе
зопасности Словацкого аграрного университета в
Нитре (Словацкая Республика) и НБС. Общее со
держание дубильных веществ определяли методом
титрования по В.П. Крищенко (водные экстракты
с индигокармином титровали раствором перман
ганата).
Результаты. Установлено, что метанольные экс
тракты растений Galega officinalis проявляли антира
дикальную активность от 19,39 % (GOFB) до 95,18 %
(GOFSR), этанольные экстракты — от 11,24 % (GOFB)
до 92,87 % (GOFF), водные экстракты — от 28,64 %
(GOFSR) до 74,63 % (GOFF), метанольные экст рак
ты растений Galega orientalis — от 20,20 % (GORSR) до
91,72 % (GORB), этанольные экстракты — от 11,74 %
(GORSR) до 84,74 % (GORF), водные экстракты —
от 22,90 % (GORSV) до 77,72 % (GORF). Общее со
держание дубильных веществ для G. officinalis составля
ло от 1,22 до 4,17 %, для G. orientalis — от 1,55 до 4,42 %
в течение вегетации.
Выводы. Растительное сырье двух видов рода Gale
ga — потенциальный источник антиоксидантов. В
период вегетации растительные экстракты Galega offi
cinalis проявляли антирадикальную активность от
11,24 до 95,18 %, Galega orientalis — от 11,74 до 91,72 % в
зависимости от экстракта и фазы развития в усло виях
НБС. Генеративные органы характеризовались более
низким содержанием дубильных веществ, чем вегета
тивные.
Ключевые слова: Galega, антиоксидантная активность,
2,2дифенил1пикрилгидразил (ДФПГ), дубильные
вещества.
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| id | oai:ojs2.plantintroduction.org:article-20 |
| institution | Plant Introduction |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2025-07-17T12:38:57Z |
| publishDate | 2018 |
| publisher | M.M. Gryshko National Botanical Garden of the NAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | wwwplantintroductionorg/e8/f1cafc4285bee9c76dcb3ba69082d9e8.pdf |
| spelling | oai:ojs2.plantintroduction.org:article-202019-11-11T08:15:12Z Antiradical activity of plant extracts of Galega officinalis L. and G. orientalis Lam. Антирадикальна активність рослинних екстрактів Galega officinalis L. та G. orientalis Lam. Shymanska, O.V. Vergun, O.M. Rakhmetov, D.B. Brindza, J. Objective – to estimate the antiradical scavenging ability of extracts of plants of Galega officinalis L. and G. orientalis Lam., depending on phase of growing in the conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG). Material and methods. Plant material of this investigation was two species of Galega L. (G. officinalis and G. orientalis) collected from experimental collection of Cultural Flora Department of NBG: GOFSV (G. officinalis, spring vegetation), GOFB (G. officinalis, budding stage), GOFF (G. officinalis, flowering stage), GOFSR (G. officinalis, seed ripening stage), GORSV (G. orientalis, spring vegetation), GORB (G. orientalis, budding stage), GORF (G. orientalis, flowering stage), GORSR (G. orientalis, seed ripening stage). The antiradical activity of methanol, ethanol and aqueous extracts, based on the discoloration reaction on the solution of DPPH (2,2-diphenyl-1-picrylhydrazyl free radical), was determined by spectrophotometric method according to Brandt-Williams et al. Biochemical preparation and analyze was carried out in the Institute of Biodiversity Conservation and Biosafety, the Slovak University of Agriculture in Nitra (Slovak Republic) and NBG. The total content of tannins was determined by titrimetric method according to Krischenko (water extracts with indigo carmine were titrated by permanganate solution). Results. We determined that methanol extracts of Galega officinalis plants had antiradical activity in range from 19.39 % (GOFB) to 95.18 % (GOFSR), ethanol extracts – from 11.24 % (GOFB) to 92.87 % (GOFF), and water extracts – from 28.64 % (GOFSR) to 74.63 % (GOFF). Methanol extracts of Galega orientalis plants had antiradical activity from 20.20 % (GORSR) to 91.72 % (GORB), ethanol extracts – from 11.74 % (GORSR) to 84.74 % (GORF), and water extracts – from 22.90 % (GORSV) to 77.72 % (GORF). The total content of tannins for G. officinalis was in range of 1.22 to 4.17 % and for G. orientalis – from 1.55 to 4.42 % during vegetation. Conclusions. Plant raw material of two Galega L. species is potential source of antioxidants. During vegetation antiradical activity of plant extracts of Galega officinalis exhibited 11.24–95.18 % and Galega orientalis – 11.74–91.72 % depending on extract and phase of growing in conditions of NBG. Generative organs such as flowers and fruits had less content of tannins than vegetative. Мета – дослідити антирадикальну дію екстрактів рослин Galega officinalis L. та G. orientalis Lam. залежно від фази розвитку в умовах Національного ботанічного саду імені М.М. Гришка НАН України (НБС). Матеріал та методи. Рослинний матеріал дослідження – два види роду Galega L. (G. officinalis та G. orientalis), зібрані з експериментальної колекції відділу культурної флори НБС: GOF-SV (G. officinalis, весняне відростання), GOF-B (G. officinalis, бутонізація), GOF-F (G. officinalis, цвітіння), GOF-SR (G. officinalis, дозрівання насіння), GOR-SV (G. orientalis, весняне відростання), GOR-B (G. orientalis, бутонізація), GOR-F (G. orientalis, цвітіння), GOR-SR (G. orientalis, дозрівання насіння). Антирадикальну активність метанольних, етанольних та водних екстрактів, яка ґрунтується на реакції знебарвлення розчину ДФПГ (2,2-дифеніл-1-пікрилгідразил вільний радикал), визначали спектрофотометричним методом за W. Brandt-Williams et al. Біохімічний аналіз проведено в Інституті збереження біорізноманіття та біологічної безпеки Словацького аграрного університету в Нітрі (Словацька Республіка) та НБС. Загальний вміст дубильних речовин визначено методом титрування за В.П. Крищенком (водні екстракти з індигокарміном титрували розчином перманганату). Результати. Визначено, що метанольні екстракти рослин Galega officinalis виявляли антирадикальну активність від 19,39 % (GOF-B) до 95,18 % (GOF-SR), етанольні екстракти – від 11,24 % (GOF-B) до 92,87 % (GOF-F), водні екстракти – від 28,64 % (GOF-SR) до 74,63 % (GOF-F), метанольні екстракти рослин Galega orientalis – від 20,20 % (GOR-SR) до 91,72 % (GOR-B), етанольні екстракти – від 11,74 % (GOR-SR) до 84,74 % (GOR-F), водні екстракти – від 22,90 % (GOR-SV) до 77,72 % (GOR-F). Загальний вміст дубильних речовин для G. officinalis становив від 1,22 до 4,17 %, для G. orientalis – від 1,55 до 4,42 % протягом вегетації. Висновки. Рослинна сировина двох видів роду Galega – потенційне джерело антиоксидантів. Протягом вегетації рослинні екстракти Galega officinalis виявляли антирадикальну активність від 11,24 до 95,18 %, Galega orientalis – від 11,74 до 91,72 % залежно від екстракту та фази розвитку в умовах НБС. Генеративні органи характеризувалися нижчим вмістом дубильних речовин, ніж вегетативні. M.M. Gryshko National Botanical Garden of the NAS of Ukraine 2018-05-01 Article Article application/pdf https://www.plantintroduction.org/index.php/pi/article/view/20 10.5281/zenodo.2229075 Plant Introduction; Vol 78 (2018); 12-19 Інтродукція Рослин; Том 78 (2018); 12-19 2663-290X 1605-6574 10.5281/zenodo.3377684 en https://www.plantintroduction.org/index.php/pi/article/view/20/15 Copyright (c) 2018 The Author(s) http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Shymanska, O.V. Vergun, O.M. Rakhmetov, D.B. Brindza, J. Антирадикальна активність рослинних екстрактів Galega officinalis L. та G. orientalis Lam. |
| title | Антирадикальна активність рослинних екстрактів Galega officinalis L. та G. orientalis Lam. |
| title_alt | Antiradical activity of plant extracts of Galega officinalis L. and G. orientalis Lam. |
| title_full | Антирадикальна активність рослинних екстрактів Galega officinalis L. та G. orientalis Lam. |
| title_fullStr | Антирадикальна активність рослинних екстрактів Galega officinalis L. та G. orientalis Lam. |
| title_full_unstemmed | Антирадикальна активність рослинних екстрактів Galega officinalis L. та G. orientalis Lam. |
| title_short | Антирадикальна активність рослинних екстрактів Galega officinalis L. та G. orientalis Lam. |
| title_sort | антирадикальна активність рослинних екстрактів galega officinalis l. та g. orientalis lam. |
| url | https://www.plantintroduction.org/index.php/pi/article/view/20 |
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