Визначення летких речовин в плодах Diospyros virginiana L.
Objective – to investigate the qualitative and quantitative content of volatile organic compounds in American persimmons (Diospyros virginiana L.) fruits of the collection of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. The objects of study were fruits of 10 ye...
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M.M. Gryshko National Botanical Garden of the NAS of Ukraine
2018
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| author | Grygorieva, O.V. Klymenko, S.V. Ilyinska, A.P. Onyshchuk, L.M. |
| author_facet | Grygorieva, O.V. Klymenko, S.V. Ilyinska, A.P. Onyshchuk, L.M. |
| author_sort | Grygorieva, O.V. |
| baseUrl_str | https://www.plantintroduction.org/index.php/pi/oai |
| collection | OJS |
| datestamp_date | 2019-11-11T08:15:36Z |
| description | Objective – to investigate the qualitative and quantitative content of volatile organic compounds in American persimmons (Diospyros virginiana L.) fruits of the collection of M.M. Gryshko National Botanical Garden of the NAS of Ukraine.
Material and methods. The objects of study were fruits of 10 years old plants of 7 genotypes (DV01–DV07) of Diospyros virginiana which were collected in October 2015. The investigation of the volatiles was conducted by the method of Chernohorod and Vinohradov (2006) using chromatographymass spectrometry. Basic statistical analyses were performed using PAST 2.17; hierarchical cluster analyses of similarity between genotypes were computed on the basis of the Bray-Curtis similarity index.
Results. During the analysis of qualitative composition and quantitative content of volatiles of fruits from the 7 genotypes 129 compounds were detected, and 118 compounds among them were identified. Total content of volatile compounds was from 121.60 to 676.50 mg/kg. Identified compounds belong to hydrocarbons, aldehydes, carboxylic acids and their ethers, monoterpenes, sesquiterpenes, triterpenes. In the fruits of investigated genotypes prevailed fatty acids (mg/kg): myristic acid (from 18.5 to 234.6), palmitic acid (from 14.9 to 125.7), lauric acid (from 29.8 to 50.5), 7,10,13-hexadecatrienic acid (from 18.6 to 33.4), 11-hexadecenoic acid (from 12.3 to 22.1). For all investigated genotypes 14 components were typical: furfural, linalool, phenylacetaldehyde, α-terpineol, geraniol, lauric acid, ethylmyristate, myristic acid, pentadecanoic acid, ethyl palmitate, ethyl palmitoleate, palmitic acid, palmitoleic acid, and squalene.
Conclusions. Detected volatile compounds in the fruits of Diospyros virginiana belong to alcohols, saturated and unsaturated aldehydes, ketones, fatty acids, esters, and terpenoids. Shares of keeping them in total identified fruits volatile substances vary and depend on the genotype of plants. The fruits are rich in fatty acids. They are considered as precursors of many specific aroma compounds. Found aldehydes are thought to be responsible for the decreasing of astringency by persimmon fruits. Study of the quantitative and qualitative content of volatile compounds of Diospyros virginiana fruits suggest about making sense of conducting further pharmacognostic investigations. |
| doi_str_mv | 10.5281/zenodo.2576119 |
| first_indexed | 2025-07-17T12:42:01Z |
| format | Article |
| fulltext |
89ISSN 16056574. Інтродукція рослин, 2018, № 4
UDC 582.687.46:581.192
O.V. GRYGORIEVA, S.V. KLYMENKO, A.P. ILYINSKA, L.M. ONYSHCHUK
M.M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine
Ukraine, 01014 Kyiv, Timiryazevska str., 1
DETERMINATION OF VOLATILE COMPOUNDS
IN FRUITS OF DIOSPYROS VIRGINIANA L.
Objective — to investigate the qualitative and quantitative content of volatile organic compounds in American persimmons
(Diospyros virginiana L.) fruits of the collection of M.M. Gryshko National Botanical Garden of the NAS of Ukraine.
Material and methods. The objects of study were fruits of 10 years old plants of 7 genotypes (DV01 — DV07) of Diospyros
virginiana which were collected in October 2015. The investigation of the volatiles was conducted by the method of Chernohorod
and Vinohradov (2006) using chromatographymass spectrometry. Basic statistical analyses were performed using PAST 2.17;
hierarchical cluster analyses of similarity between genotypes were computed on the basis of the Bray—Curtis similarity index.
Results. During the analysis of qualitative composition and quantitative content of volatiles of fruits from the 7 genotypes 129
compounds were detected, and 118 compounds among them were identified. Total content of volatile compounds was from 121.60
to 676.50 mg/kg. Identified compounds belong to hydrocarbons, aldehydes, carboxylic acids and their ethers, monoterpenes,
sesquiterpenes, triterpenes. In the fruits of investigated genotypes prevailed fatty acids (mg/kg): myristic acid (from 18.5 to
234.6), palmitic acid (from 14.9 to 125.7), lauric acid (from 29.8 to 50.5), 7,10,13hexadecatrienic acid (from 18.6 to 33.4),
11hexadecenoic acid (from 12.3 to 22.1). For all investigated genotypes 14 components were typical: furfural, linalool,
phenylacetaldehyde, Q terpineol, geraniol, lauric acid, ethylmyristate, myristic acid, pentadecanoic acid, ethyl palmitate, ethyl
palmitoleate, palmitic acid, palmitoleic acid, and squalene.
Conclusions. Detected volatile compounds in the fruits of Diospyros virginiana belong to alcohols, saturated and unsaturated
aldehydes, ketones, fatty acids, esters, and terpenoids. Shares of keeping them in total identified fruits volatile substances vary
and depend on the genotype of plants. The fruits are rich in fatty acids. They are considered as precursors of many specific aroma
compounds. Found aldehydes are thought to be responsible for the decreasing of astringency by persimmon fruits. Study of the
quantitative and qualitative content of volatile compounds of Diospyros virginiana fruits suggest about making sense of conducting
further pharmacognostic investigations.
Key words: Diospyros virginiana, ForestSteppe of Ukraine, fruits, volatile compounds.
© O.V. GRYGORIEVA, S.V. KLYMENKO,
A.P. ILYINSKA, L.M. ONYSHCHUK, 2018
Introduction
The deterioration of ecological situation in the
world caused the need for finding of new plant
species which is valuable source of biologically ac
tive compounds. It is source of prevention and
treatment for human health by natural products of
organic origin, the socalled nontraditional plant
species: Aronia Mitschurinii A.K. Skvortsov & Mai
tul., Cornus mas L., Cydonia oblonga Mill., Diospy
ros spp., Elaeagnus multiflora Thunb., Morus nigra L.,
Pseudocydonia sinensis Schneid., Ziziphus jujuba
Mill. [6; 12; 15; 16; 23; 25; 26; 28; 33; 44].
American persimmons (Diospyros virginiana L.)
are known as a widespread culture in a traditional use
by Native Americans as food product [5; 10; 18; 31; 39].
The natural range of Diospyros virginiana includes
the eastern part of North America from Connecticut
to Iowa and from Kansas to Florida [9; 41]. Today
more than 200 cultivars of Diospyros virginiana exist
and their fruits have differences in fruits shape, size,
color and ripening [11; 13; 14; 17; 42; 46].
The Diospyros virginiana is of great practical in
terest for fruit growing. The fruits of American per
simmon are an excellent dietary product, they are
used in fresh condition and from them are pre
pared pastes, jams, syrups, marinades. The fruits
were also used to make wine, brandy, white wine
vinegar and beer [3; 5]. In addition, the American
persimmon is a valuable decorative and medicinal
plant. Diospyros virginiana since ancient times is
used in the folk medicine [5; 10; 18; 31]. The fruit
has been used medicinally as antiseptic and for the
treatment of burns, diphtheria, dropsy, diarrhoea,
90 ISSN 16056574. Інтродукція рослин, 2018, № 4
O.V. Grygorieva, S.V. Klymenko, A.P. Ilyinska, L.M. Onyshchuk
Fig. 1. Chromatogram of volatiles com
pounds of fruits of Diospyros virginiana L.
genotypes
91ISSN 16056574. Інтродукція рослин, 2018, № 4
Determination of volatile compounds in fruits of Diospyros virginiana L.
0
10
20
30
40
50
60
70
DV-01 DV-02 DV-03 DV-04 DV-05 DV-06 DV-07
Total volatiles compounds
Identified volatiles
compounds
Fig. 2. The number of volatiles compounds (total and
identified) of Diospyros virginiana L. genotypes
gonorrhoea, candidiasis, dysentery, fevers, thrush,
fungal and bacterial infections, gastrointestinal
bleeding, sore throats [5]. Fruits exhibit the anti
microbial, antifungal [38] and antioxidant activi
ties [16]. Fruits and leaves have an antitumor [40],
antimicrobial [20; 22], and antifungal effects [45].
The bark has an antiseptic [5], hepatoprotective
and antipyretic [36; 37] action.
Objective — to investigate the qualitative and
quantitative content of volatile organic compounds
in American persimmons (Diospyros virginiana L.)
fruits of the collection of M.M. Gryshko National
Botanical Garden of the NAS of Uk raine.
Material and methods
Locating trees and data collection
The fruits of 7 genotypes of Diospyros virginiana
(DV01—DV07) collected in the M.M. Gryshko
National Botanical Garden of NAS of Ukraine
(NBG) were the objects of these investigations.
The raw material was collected in the period (Oc
tober) of full ripeness.
Volatile compounds analysis
The investigation of the volatiles was conducted at
the National Institute of Viticulture and Wine “Maga
rach” by the method of Chernogorod and Vino
hradov (2006) [21]. Volatiles were investigated by
the method of chromatographymass spectrometry
using the chromatograph Agilent Technologies 6890 N
with the mass spectrometric detector 5973 N (USA)
and a capillary column DB5 length is 30 mm and an
internal diameter is 0.25 mm. The carrier gas velocity
(Helium) was 1.2 ml/min. The injector heater tem
perature was 250 °C. The temperature of termostate
was programmed from 50 °C to 320 °C at the speed
4 °C. The mass spectra library NIST 05 WILEY
2007 with 470 000 spectra and AMDIS, NIST pro
grams were used to identify the investigated com
pounds. The identification was conducted by com
paring obtained mass spectra to mass spectra of
standards. The method of internal standad used
to determine the quantitative content of compounds.
Statistical analyses
Basic statistical analyses were performed using
PAST 2.17; hierarchical cluster analyses of simi
larity between phenotypes were computed on the
basis of the BrayCurtis similarity index.
Results and discussion
Plants emit a great diversity of volatile compounds
from leaves, bark, roots, flowers, and fruits [7; 8;
24; 27; 29; 30; 35].
The volatile compounds have remained an over
looked trait in plant phenotyping [30] that enables
the identification of specific chemotypic profiles
among and within species [34].
In this study, 129 volatiles compounds in the
fruits of Diospyros virginiana were detected. Among
them, 118 compounds were identified.
The chromatogram of volatiles compounds of
fruits of selected genotypes of Diospyros virginiana
is represented in Fig. 1.
The identified components belong to different
chemical classes, including hydrocarbons, alco
hols, aldehydes and phenylaldehydes, terpenes,
esters and fatty acids.
The widest spectrum of volatiles compounds
was characterized by the fruits of genotype DV02
(74 compounds), among which were identified 64
substances (Fig. 2).
Nonidentified compounds have peak with square
no more 0.4 % and respectively extremely low inten
sity of massspectrums, which not allows achiev
ing the required reliability of results in the librarian
searching.
Fruits of DV04 genotype had the higher con
tent of total volitile compounds (676.5 mg/kg),
and fruits of DV02 genotype — the least one
(121.6 mg/kg) (Fig. 3).
Obtained results of a content of total volatile
compounds were confirmed by data of cluster
92 ISSN 16056574. Інтродукція рослин, 2018, № 4
O.V. Grygorieva, S.V. Klymenko, A.P. Ilyinska, L.M. Onyshchuk
DV-01
DV-02
DV-03
DV-04
DV-05
DV-06
DV-07
0 200 400 600
Total volatile compounds, mg/kg
Fig. 3. The total volatile compounds of fruits of Diospyros
virginiana L. genotypes
DV-05
DV-01
DV-06
DV-02
DV-04
DV-03
DV-07
Genotypes
30 60 90 120 150 180 210 240 270
Total volatile compounds, mg/kg
Fig. 4. Cluster diagram of volatile compounds of fruits of
Diospyros virginiana L. genotypes
analysis (Fig. 4). The cluster analysis (CA) was
performed according to the hierarchical cluster
analysis (HCA) method using the mean value to
distinguish similar groups among the various vola
tiles compounds. In this study, seven genotypes
were grouped into the two main clusters based on
highest similarities. In Group 1 concluded geno
types (DV03, DV04 and DV07) with the biggest
content of volatile compounds. Rest genotypes
concluded in the Group 2.
Common for all investigated genotypes was the
presence of 14 components: furfural, linalool,
phenylacetaldehyde, R terpineol, geraniol, lauric
acid, ethylmyristate, myristic acid, pentadecanoic
acid, ethyl palmitate, ethyl palmitoleate, palmitic
acid, palmitoleic acid, and squalene.
Among the fatty acids of genotypes fruits were
found myristic acid from 15.21 (DV02) to 43.45
(DV06) %, palmitic acid from 6.34 (DV06) to
29.09 (DV05) %, lauric acid from 3.45 (DV02)
to 12.27 (DV01) %, palmitoleic acid from 3.28
(DV06) to 11.03 (DV0) %, and 7,10,13hexade
catrienic acid from 3.29 (DV02) to 6.57 (DV03) %
(Fig. 5). The last component wasn’t identified in
the genotypes of DV05 and DV06, but presence
11hexadecenoic acid in the range from 6.85
(DV05) to 7.94 (DV06) %. The share of other
components varied from 14.69 (DV07) to 65.38
(DV02) %.
Palmitic acid was identified as minor constituents
by Horvat et al. (1991) [19]. Among other saturated
fatty acids were identified butyric, caproic (hexano
ic), caprylic (octanoic), nonanoic, capric (deca
noic), lauric (dodecanoic), tridecanoic, pentade
canoic ones. Also, we identified that some geno
types contain stearic acid C
17
H
35
COOH and it’s
unsaturated derivatives: oleic acid C
17
H
33
COOH
(one double bond), linoleic acid C
17
H
31
COOH (two
double bonds) and linolenic acid C
17
H
29
COOH
(three double bonds). Later they were identified in
all genotypes.
In the present study, isoamyl alcohol and hepta
nol4 (DV01), octanol (DV01, DV02, DV03),
benzyl alcohol (DV03, DV04, DV05, DV07)
were identified.
According to Besada et al. (2013) [4], the high
accumulation of phenylacetaldehyde and lipid
derived aldehydes are related to loss of astringency
of fruits. Regarding to the previously described
vo latile compounds of the Diospyros kaki, Besada
et al. (2013) [4] benzyl alcohol and some related
compounds such as acetaldehyde, hexanol1, 3me
93ISSN 16056574. Інтродукція рослин, 2018, № 4
Determination of volatile compounds in fruits of Diospyros virginiana L.
DV-05
DV-01
DV-06
DV-02
DV-04
DV-03
DV-07
6
(30.57%)
6
(63.38%)
6
(26.34%)
6
(27.86%)
6
(28.84%)
6
(24.91%)
6
(14.69%)
1
(5.91%)
1
(3.29%)
1
(6.57%)
1
(4.94%)
1
(5.04%)
2
(10.88%)
3
(11.03%)
4
(18.31%)
5
(40.06%)
2
(7.35%)
3
(9.55%)
4
(18.58%)
5
(34.68%) 5
(27.79%)
4
(29.09%)
3
(7.43%)
2
(3.59%)
7
(6.85%)
7
(7.94%)
2
(11.12%)
3
(3.28%)
4
(6.34%)
5
(43.45%)
2
(7.73%)
3
(6.88%)
4
(22.65%)
5
(30.14%)
2
(3.45%)
3
(3.87%)
4
(12.25%)
5
(15.21%)
2
(12.27%)
3
(5.40%)
4
(13.76%)
5
(32.09%)
Fig. 5. The fatty acid content of Diospyros virginiana L. genotypes: 1 — 7, 10,
13hexadecatrienic acid; 2 — lauric acid; 3 — рalmitoleic acid; 4 — palmitic
acid; 5 — myristic acid; 6 — оther components; 7 — 11hexadecenoic acid
thyl1heptanol, 1undecanol, and aliphatic satu
rated and unsaturated aldehydes such as hexanal,
heptanal, octanal, decanal, (E)2octenal, (Z)2
nonenal, (E)2decenal, (E,E)2,4heptadienal
were identified. Taira et al. (1995) identified such
volatile compounds of astringent Diospyros kaki
94 ISSN 16056574. Інтродукція рослин, 2018, № 4
O.V. Grygorieva, S.V. Klymenko, A.P. Ilyinska, L.M. Onyshchuk
fruits as nbutanol, hehanol1, (Z)3hexen1ol,
2methyl hexanol, acetoin and acetic acid [43].
Flavour and aroma are important quality fea
tures in American persimmon fruits. Flavour is
formed by the combination of sweetness and sour
ness from carbohydrates, organic acids and aroma
volatile compounds [4]. In general, fruit volatile
compounds refer to aliphatic esters, alcohols, alde
hydes, ketones, lactones, terpenoids (monoterpe
nes, sesquiterpenes) and apocarotenoids. Fatty ac
ids are the major primary precursor substrates of
many characterimpact aroma compounds in most
fruits. Aliphatic alcohols, aldehydes, keton es, or
ganic acids, esters and lactones, ranging from C
1
to
C
20
, are all derived from fatty acid precursors through
three key biosynthetic processes: S oxi da tion, T oxi
dation and the lipoxygenase pathway. Sensor analy
sis is used for the estimation of the ripening stage
and storage life of Diospyros kaki fruits [2].
Among the identified volatiles responsible for
flavour in this study were linalool, S terpineol and
geraniol in all the genotypes and nerol in DV01
and DV06, terpinene4ol in DV04 and DV06
genotypes. They all belong to terpenoids. Gera
niol and nerol have a rose odour, nerol has a weak
er odour [1]. As was stated by Martineli et al.
(2013) [32] the volatiles from Diospyros kaki were
mainly represented by terpens hydrocarbons, fol
lowed by straightchain esters.
The fact that less part of identified volatile com
pounds in American persimmon flesh in this study
were reported by other scientists could be explained,
first of all by the absence of available studies for Dio
spyros virginiana. Therefore, our results were com
pared with studies performed for Diospyros ka ki.
Conclusions
Identified volatile compounds in the fruits of Dio
spyros virginiana belong to alcohols, saturated and
unsaturated aldehydes, ketones, fatty acids, esters,
and terpenoids. Shares of keeping them in identi
fied fruits volatile substances vary and depend on
the genotype of plants. The fruits are rich in fatty
acids. They are considered as precursors of many
specific aroma compounds. Aldehydes are thought
to be responsible for the loss of astringency by per
simmon fruits.
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Recommended by J. Brindza, O.M. Vergun
Received 14.07.2018
О.В. Григор’єва, С.В. Клименко,
А.П. Ільїнська, Л.М. Онищук
Національний ботанічний сад
імені М.М. Гришка НАН України,
Україна, м. Київ
ВИЗНАЧЕННЯ ЛЕТКИХ РЕЧОВИН
В ПЛОДАХ DIOSPYROS VIRGINIANA L.
Мета — дослідити якісний склад і кількісний вміст
летких речовин у плодах хурми віргінської (Diospyros
virginiana L.) з колекції Національного ботанічного
саду імені М.М. Гришка НАН України.
Матеріал та методи. Об’єктом дослідження були
плоди 7 генотипів (DV01—DV07) 10річних рослин
Diospyros virginiana, заготовлені у жовтні 2015 p. До
слідження летких речовин проводили за методикою
Черногорода та Виноградова (2006) з використанням
хроматомасспектрометрії. Основні статистичні ана
лізи виконували за допомогою PAST 2.17. Ієрархічний
кластерний аналіз подібності між генотипами обчис
лено за індексом подібності Брей—Кертіса.
Результати. У плодах 7 генотипів визначено 129 ком
понентів, з них ідентифіковано 118. Сумарний кіль
кісний вміст летких компонентів становив від 121,60
до 676,50 мг/кг. Визначені сполуки належать до вугле
воднів, альдегідів, карбонових кислот та їх ефірів, мо
нотерпенів, сесквитерпенів, тритерпенів. В плодах до
сліджуваних генотипів переважають жирні кислоти
(мг/кг): міристинова (від 18,5 до 234,6), пальмітинова
(від 14,9 до 125,7), лауринова (від 29,8 до 50,5), 7,10,
13гексадекатринова (від 18,6 до 33,4), 11гек са де це но ва
(від 12,3 до 22,1). Для всіх досліджуваних гено типів були
характерні 14 компонентів: фурфурол, ліна лоол, феніл
ацетальдегід, _ терпінеол, гераніол, лау ри но ва кислота,
етилмеристат, міристинова кислота, пентадеканоїнова
кислота, етилпальмітат, етилпальмітолеат, пальміти
нова кислота, пальмітолеїнова кислота та сквален.
Висновки. Визначені леткі сполуки в плодах Dio
spyros virginiana належать до спиртів, насичених та не
насичених альдегідів, кетонів, жирних кислот, ефірів
та терпеноїдів. Їх частка в ідентифікованих летких
речовинах плодів варіює та залежить від генотипу.
Плоди багаті на жирні кислоти. Їх вважають прекур
сорами багатьох специфічних ароматичних сполук.
Виявлені альдегіди сприяють зменшенню терпкості в
плодах хурми. Дослідження якісного складу та кіль
кісного вмісту летких компонентів плодів Diospyros vir
giniana свідчить про доцільність проведення подаль
ших фармакогностичних досліджень.
Ключові слова: Diospyros virginiana, Лісостеп України,
плоди, леткі речовини.
О.В. Григорьева, С.В. Клименко,
А.П. Ильинская, Л.Н. Онищук
Национальный ботанический сад
имени Н.Н. Гришко НАН Украины,
Украина, г. Киев
ОПРЕДЕЛЕНИЕ ЛЕТУЧИХ ВЕЩЕСТВ
В ПЛОДАХ DIOSPYROS VIRGINIANA L.
Цель — исследовать качественный состав и количествен
ное содержание летучих веществ в плодах хурмы виргин
ской (Diospyros virginiana L.) из коллекции Национального
ботанического сада имени Н.Н. Гришко НАН Украины.
97ISSN 16056574. Інтродукція рослин, 2018, № 4
Determination of volatile compounds in fruits of Diospyros virginiana L.
Материал и методы. Объектом исследования были
плоды 7 генотипов (DV01—DV07) 10летних расте
ний Diospyros virginiana, заготовленные в октябре 2015 г.
Исследование летучих веществ проводили по методике
Черногорода и Виноградова (2006) с использованием
хроматомассспектрометрии. Основные статисти чес кие
анализы выполняли с помощью PAST 2.17. Иерархиче
ский кластерный анализ сходства между генотипами
рассчитан на основе индекса сходства Брей— Кертиса.
Результаты. В плодах 7 генотипов определены 129 ком
понентов, из них идентифицированы 118. Суммарное
количественное содержание летучих компонентов
составляло от 121,60 до 676,50 мг/кг. Идентифициро
ванные соединения относятся к углеводородам, альде
гидам, карбоновым кислотам и их эфирам, монотер
пенам, сесквитерпенам, тритерпенам. В плодах ис
следуемых генотипов преобладают жирные кислоты
(мг/кг): миристиновая (от 18,5 до 234,6), пальмити
новая (от 14,9 до 125,7), лауриновая (от 29,8 до 50,5),
7,10,13гексадекатриновая (от 18,6 до 33,4), 11гек са
де ценовая (от 12,3 до 22,1). Для всех исследуемых гено
типов были характерны 14 компонентов: фурфурол,
линалоол, фенилацетальдегид, ` тер пи не ол, гераниол,
лауриновая кислота, этилмеристат, миристиновая кис
лота, пентадеканоиновая кислота, этил паль митат, этил
пальмитолеат, пальмитиновая кис лота, пальмитолеи
новая кислота и сквален.
Выводы. Идентифицированные летучие соедине
ния в плодах Diospyros virginiana принадлежат к спир
там, насыщенным и ненасыщенным альдегидам, ке
тонам, жирным кислотам, эфирам и терпеноидам. Их
доля в идентифицированных летучих веществах пло
дов варьирует и зависит от генотипа. Плоды богаты
жир ными кислотами. Их считают прекурсорами мно
гих специфических ароматических соединений.
Идентифицированные альдегиды способствуют
уменьшению терпкости в плодах хурмы. Исследова
ние качественного состава и количественного содер
жания ле тучих компонентов плодов Diospyros virginia
na свидетельст вует о целесообразности проведения
дальнейших фармакогностических исследований.
Ключевые слова: Diospyros virginiana, Лесостепь Украи ны,
плоды, летучие вещества.
|
| id | oai:ojs2.plantintroduction.org:article-363 |
| institution | Plant Introduction |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2025-07-17T12:42:01Z |
| publishDate | 2018 |
| publisher | M.M. Gryshko National Botanical Garden of the NAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | wwwplantintroductionorg/70/12bce03619ed8c5bb2ab93389c9e5170.pdf |
| spelling | oai:ojs2.plantintroduction.org:article-3632019-11-11T08:15:36Z Determination of volatile compounds in fruits of Diospyros virginiana L. Визначення летких речовин в плодах Diospyros virginiana L. Grygorieva, O.V. Klymenko, S.V. Ilyinska, A.P. Onyshchuk, L.M. Objective – to investigate the qualitative and quantitative content of volatile organic compounds in American persimmons (Diospyros virginiana L.) fruits of the collection of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. The objects of study were fruits of 10 years old plants of 7 genotypes (DV01–DV07) of Diospyros virginiana which were collected in October 2015. The investigation of the volatiles was conducted by the method of Chernohorod and Vinohradov (2006) using chromatographymass spectrometry. Basic statistical analyses were performed using PAST 2.17; hierarchical cluster analyses of similarity between genotypes were computed on the basis of the Bray-Curtis similarity index. Results. During the analysis of qualitative composition and quantitative content of volatiles of fruits from the 7 genotypes 129 compounds were detected, and 118 compounds among them were identified. Total content of volatile compounds was from 121.60 to 676.50 mg/kg. Identified compounds belong to hydrocarbons, aldehydes, carboxylic acids and their ethers, monoterpenes, sesquiterpenes, triterpenes. In the fruits of investigated genotypes prevailed fatty acids (mg/kg): myristic acid (from 18.5 to 234.6), palmitic acid (from 14.9 to 125.7), lauric acid (from 29.8 to 50.5), 7,10,13-hexadecatrienic acid (from 18.6 to 33.4), 11-hexadecenoic acid (from 12.3 to 22.1). For all investigated genotypes 14 components were typical: furfural, linalool, phenylacetaldehyde, α-terpineol, geraniol, lauric acid, ethylmyristate, myristic acid, pentadecanoic acid, ethyl palmitate, ethyl palmitoleate, palmitic acid, palmitoleic acid, and squalene. Conclusions. Detected volatile compounds in the fruits of Diospyros virginiana belong to alcohols, saturated and unsaturated aldehydes, ketones, fatty acids, esters, and terpenoids. Shares of keeping them in total identified fruits volatile substances vary and depend on the genotype of plants. The fruits are rich in fatty acids. They are considered as precursors of many specific aroma compounds. Found aldehydes are thought to be responsible for the decreasing of astringency by persimmon fruits. Study of the quantitative and qualitative content of volatile compounds of Diospyros virginiana fruits suggest about making sense of conducting further pharmacognostic investigations. Мета – дослідити якісний склад і кількісний вміст летких речовин у плодах хурми віргінської (Diospyros virginiana L.) з колекції Національного ботанічного саду імені М.М. Гришка НАН України. Матеріал та методи. Об’єктом дослідження були плоди 7 генотипів (DV-01–DV-07) 10-річних рослин Diospyros virginiana, заготовлені у жовтні 2015 p. Дослідження летких речовин проводили за методикою Черногорода та Виноградова (2006) з використанням хромато-мас-спектрометрії. Основні статистичні аналізи виконували за допомогою PAST 2.17. Ієрархічний кластерний аналіз подібності між генотипами обчислено за індексом подібності Брей–Кертіса. Результати. У плодах 7 генотипів визначено 129 компонентів, з них ідентифіковано 118. Сумарний кількісний вміст летких компонентів становив від 121,60 до 676,50 мг/кг. Визначені сполуки належать до вуглеводнів, альдегідів, карбонових кислот та їх ефірів, монотерпенів, сесквитерпенів, тритерпенів. В плодах досліджуваних генотипів переважають жирні кислоти (мг/кг): міристинова (від 18,5 до 234,6), пальмітинова (від 14,9 до 125,7), лауринова (від 29,8 до 50,5), 7,10, 13-гексадекатринова (від 18,6 до 33,4), 11-гексадеценова (від 12,3 до 22,1). Для всіх досліджуваних генотипів були характерні 14 компонентів: фурфурол, ліналоол, феніл-ацетальдегід, а-терпінеол, гераніол, лауринова кислота, етилмеристат, міристинова кислота, пентадеканоїнова кислота, етилпальмітат, етилпальмітолеат, пальмітинова кислота, пальмітолеїнова кислота та сквален. Висновки. Визначені леткі сполуки в плодах Diospyros virginiana належать до спиртів, насичених та ненасичених альдегідів, кетонів, жирних кислот, ефірів та терпеноїдів. Їх частка в ідентифікованих летких речовинах плодів варіює та залежить від генотипу. Плоди багаті на жирні кислоти. Їх вважають прекурсорами багатьох специфічних ароматичних сполук. Виявлені альдегіди сприяють зменшенню терпкості в плодах хурми. Дослідження якісного складу та кількісного вмісту летких компонентів плодів Diospyros virginiana свідчить про доцільність проведення подальших фармакогностичних досліджень. M.M. Gryshko National Botanical Garden of the NAS of Ukraine 2018-12-01 Article Article application/pdf https://www.plantintroduction.org/index.php/pi/article/view/363 10.5281/zenodo.2576119 Plant Introduction; Vol 80 (2018); 89-97 Інтродукція Рослин; Том 80 (2018); 89-97 2663-290X 1605-6574 10.5281/zenodo.3377676 en https://www.plantintroduction.org/index.php/pi/article/view/363/345 http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Grygorieva, O.V. Klymenko, S.V. Ilyinska, A.P. Onyshchuk, L.M. Визначення летких речовин в плодах Diospyros virginiana L. |
| title | Визначення летких речовин в плодах Diospyros virginiana L. |
| title_alt | Determination of volatile compounds in fruits of Diospyros virginiana L. |
| title_full | Визначення летких речовин в плодах Diospyros virginiana L. |
| title_fullStr | Визначення летких речовин в плодах Diospyros virginiana L. |
| title_full_unstemmed | Визначення летких речовин в плодах Diospyros virginiana L. |
| title_short | Визначення летких речовин в плодах Diospyros virginiana L. |
| title_sort | визначення летких речовин в плодах diospyros virginiana l. |
| url | https://www.plantintroduction.org/index.php/pi/article/view/363 |
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