Антиоксидантний потенціал рослин родин Brassicaceae Burnett та Poaceae Barnhart.
Objective – to estimate the antiradical scavenging ability of extracts of some plants of Brassicaceae Burnett and Poaceae Barnhart. species, to evaluate the total antioxidant activity of plants in the conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. P...
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| Дата: | 2018 |
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| Автори: | , |
| Формат: | Стаття |
| Мова: | Англійська |
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M.M. Gryshko National Botanical Garden of the NAS of Ukraine
2018
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Репозитарії
Plant Introduction| _version_ | 1860120857415778304 |
|---|---|
| author | Vergun, O.M. Rakhmetov, D.B. |
| author_facet | Vergun, O.M. Rakhmetov, D.B. |
| author_sort | Vergun, O.M. |
| baseUrl_str | https://www.plantintroduction.org/index.php/pi/oai |
| collection | OJS |
| datestamp_date | 2019-11-11T08:15:00Z |
| description | Objective – to estimate the antiradical scavenging ability of extracts of some plants of Brassicaceae Burnett and Poaceae Barnhart. species, to evaluate the total antioxidant activity of plants in the conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine.
Material and methods. Plant material of this investigation – species, varieties and cultivars of Brassicaceaae and Poaceae families: Brassica campestris f. annua D.C. (BCA), B. campestris f. biennis D.C.× B. napus f. biennis D.C., cv. Innovatsiia (BCBNI), B. campestris f. biennis D.C. × B. rapa L. × B. napus f. biennis D.C. (BCBRBN), B. juncea (L.) Czern. (BJ), B. juncea (L.) Czern. f. biennis, cv. Annushka (BJA), B. napus f. biennis D.C., f. EROF-5 (BNE-5), B. rapa L. (BR), B. rapa subsp. rapifera Metzger (BRR), Raphanus sativus L. var. oleiformis Pers. (RSO), Miscanthus sacchariflorus (Maxim.) Benth., cv. Snihopad (MSS), M. sinensis Anderss. f. ESBMK-6 (MSE-6), Panicum virgatum L. f. PB (PVPB), P. virgatum L. f. PL (PVPL), Sorghum bicolor (L.) Moench. f. ETSSDF-11 (SBE-11), S. nigrum Roemer et Schultes f. ETSSCHF-1 (SNE-1), S. saccharatum (L.) Moench. cv. Medove (SSM), S. sudanense (Piper) Stapf (SS), S. technicum (Koern.) Bait. et Trab. (ST). The antioxidant activity of methanol and aqueous extracts based on the discoloration reaction on the solution of DPPH (2,2-diphenyl-1-picrylhydrazyl free radical) was determined according to Brandt-Williams et al. The determination of content of ascorbic acid was conducted according to V.P. Krishchenko, the content of carotene – according to B.P. Pleshkov.
Results. The antioxidant activity of methanol extracts of plants of Brassicaceae was registered in the range from 16.94±0.15 (BCA) to 36.91 ± 0.26 (BRR) % and aqueous extracts – from 26.53 ± 0.34 (BJ) to 65.85 ± 0.30 (RSO) %. This parameter for methanol extracts of Poaceae species was in range from 31.13 ± 0.32 (MSE-6) to 86.48 ± 0.49 (SNE-1) % and aqueous extracts – from 33.10 ± 0.47 (ST) to 83.14 ± 0.46 (PVPB) %. It was determined concentration of ascorbic acid from 118.67 ± 9.90 (BJA) to 566.61 ± 38.37 (BNE-5) mg% and carotene – from 0.48 ± 0.01 (BJA) to 3.11 ± 0.07 (BJ) mg% for Brassicaceae plants. Content of ascorbic acid in range from 11.80 ± 0.85 (PVPB) to 77.39 ± 1.29 (SS) mg% and carotene – from 0.05 ± 0.00 (PVPB) to 0.62 ± 0.02 (PVPL) mg% was identified for Poaceae plants.
Conclusions. This study showed that plant raw material of investigated plants is a valuable source of antioxidants and vitamins. Methanol and water extracts of plants of Poaceae had higher antiradical activity against DPPH radical than extracts of plants of Brassicaceae. The higher level of ascorbic acid and carotene was observed in extracts of plants of Brassicaceae. The minimal content of ascorbic acid and carotene found for BJA (Brassicaceae) and PVPB (Poaceae). |
| doi_str_mv | 10.5281/zenodo.2174708 |
| first_indexed | 2025-07-17T12:39:07Z |
| format | Article |
| fulltext |
87ISSN 1605-6574. Інтродукція рослин, 2018, № 1
UDC (582.683.2+582.542.11): 581.192
O.M. VERGUN, D.B. RAKHMETOV
M.M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine
Ukraine, 01014 Kyiv, Timiryazevska str., 1
ANTIOXIDANT POTENTIAL OF SOME PLANTS
OF BRASSICACEAE BURNETT AND POACEAE BARNHART.
Objective — to estimate the antiradical scavenging ability of extracts of some plants of Brassicaceae Burnett and Poaceae Barn-
hart. species, to evaluate the total antioxidant activity of plants in the conditions of M.M. Gryshko National Botanical Garden
of the NAS of Ukraine.
Material and methods. Plant material of this investigation — species, varieties and cultivars of Brassicaceaae and Poaceae
families: Brassica campestris f. annua D.C. (BCA), B. campestris f. biennis D.C.× B. napus f. biennis D.C., cv. Innovatsiia
(BCBNI), B. campestris f. biennis D.C. × B. rapa L. × B. napus f. biennis D.C. (BCBRBN), B. juncea (L.) Czern. (BJ), B. juncea
(L.) Czern. f. biennis, cv. Annushka (BJA), B. napus f. biennis D.C., f. EROF-5 (BNE-5), B. rapa L. (BR), B. rapa subsp.
rapifera Metzger (BRR), Raphanus sativus L. var. oleiformis Pers. (RSO), Miscanthus sacchariflorus (Maxim.) Benth., cv.
Snihopad (MSS), M. sinensis Anderss. f. ESBMK-6 (MSE-6), Panicum virgatum L. f. PB (PVPB), P. virgatum L. f. PL (PVPL),
Sorghum bicolor (L.) Moench. f. ETSSDF-11 (SBE-11), S. nigrum Roemer & Schultes f. ETSSCHF-1 (SNE-1), S. sacchara-
tum (L.) Moench. cv. Medove (SSM), S. sudanense (Piper) Stapf (SS), S. technicum (Koern.) Bait. et Trab. (ST). The antioxi-
dant activity of methanol and aqueous extracts based on the discoloration reaction on the solution of DPPH (2,2-diphenyl-
1-picrylhydrazyl free radical) was determined according to Brandt-Williams et al. The determination of content of ascorbic acid
was conducted according to V.P. Krishchenko, the content of carotene — according to B.P. Pleshkov.
Results. The antioxidant activity of methanol extracts of plants of Brassicaceae was registered in the range from 16.94±0.15 (BCA)
to 36.91 ± 0.26 (BRR) % and aqueous extracts — from 26.53 ± 0.34 (BJ) to 65.85 ± 0.30 (RSO) %. This parameter for methanol
extracts of Poaceae species was in range from 31.13 ± 0.32 (MSE-6) to 86.48 ± 0.49 (SNE-1) % and aqueous extracts —
from 33.10 ± 0.47 (ST) to 83.14 ± 0.46 (PVPB) %. It was determined concentration of ascorbic acid from 118.67 ± 9.90 (BJA)
to 566.61 ± 38.37 (BNE-5) mg% and carotene — from 0.48 ± 0.01 (BJA) to 3.11 ± 0.07 (BJ) mg% for Brassicaceae plants.
Content of ascorbic acid in range from 11.80 ± 0.85 (PVPB) to 77.39 ± 1.29 (SS) mg% and carotene — from 0.05 ± 0.00 (PVPB)
to 0.62 ± 0.02 (PVPL) mg% was identified for Poaceae plants.
Conclusions. This study showed that plant raw material of investigated plants is a valuable source of antioxidants and vitamins.
Methanol and water extracts of plants of Poaceae had higher antiradical activity against DPPH radical than extracts of plants
of Brassicaceae. The higher level of ascorbic acid and carotene was observed in extracts of plants of Brassicaceae. The minimal
content of ascorbic acid and carotene found for BJA (Brassicaceae) and PVPB (Poaceae).
Key words: Brassicaceae, Poaceae, antioxidant activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH), ascorbic acid, carotene.
© O.M. VERGUN, D.B. RAKHMETOV, 2018
Antioxidants are compounds capable to either delay
or inhibit the oxidation processes which occur under
the influence of atmospheric oxygen or reactive
oxygen species. They are used for the stabilization
of polymeric products of petrochemicals, foodstuffs,
cosmetics and pharmaceuticals [28]. In nature there
are a wide variety of naturally occurring antioxidants
which are different in their composition, physical
and chemical properties, mechanisms and site of ac-
tion. Some of the main categories can be described
as follows: enzymes, high molecular weight com-
pounds, low molecular weight compounds, miner-
als, vitamins etc. [21]. Antioxidants are involved in
the defense mechanism of the organism against the
pathologies associated with the attack of free radi-
cals. The evaluation of the total antioxidant capacity
may be an appropriate tool to determine the additive
antioxidant properties of plant foods.
There is great number of methods for determi-
nation of antioxidant capacity of foods and bever-
ages based on different principles. One of the most
widespread methods of investigation of antioxi-
dant activity is free radical scavenging activity with
DPPH radical [24].
The Brassicaceae Burnett (Cruciferae) or mus-
tard family includes many economically important
88 ISSN 1605-6574. Інтродукція рослин, 2018, № 1
O.M. Vergun, D.B. Rakhmetov
edible and industrial, oilseed, vegetable, condi-
ment and fodder crop species. The most important
edible oil crop is canola or oilseed rape (Brassica
napus); while mustard condiment crops include:
Brassica juncea — Indian mustard and Sinapis
alba — white mustard. Many Brassica species are
also important vegetable crops, e.g., cole crops
(Brassica oleracea). Several species, e.g., Brassica
carinata, Camelina sativa, Crambe abyssinica, Eru-
ca vesicaria, have potential as new edible oil/pro-
tein crops, biodiesel fuel crops, or platforms for
bioproducts or molecular farming [35]. Poaceae
Barnhart. that yield food and fodder are well
known from times immemorial and considerable
amount of research work has been carried on
them. But the grasses that yield therapeutically
important products are among the least studied in
the Poaceae family. The species of Poaceae screen-
ed for phytochemical constituents seemed to have
the potential to be source of useful drug com-
pounds and also to improve the health status of the
animals as a result of the presence of various com-
ponents that are vital for good health. These can
be incorporated in other foods as nutraceuticals
for effective and proper metabolism as well as for
the maintenance of good physiological state in
man and animals [12].
The antioxidant properties of cultivated plants are
usually well recognized. There is, however, little data
about some species from Brassicaceae and Poaceae.
Therefore further studies must be carried out.
Material and methods
Plant material was collected in M.M. Gryshko
National Botanical Garden of the NAS of Ukraine.
The species, cultivars and varieties of crop plants
belonging to Brassicaceae (9 samples) and Poaceae
(9 samples) family were used to investigate: Bras-
sica campestris f. annua D.C. (BCA), B. campestris
f. biennis D.C.× B. napus f. biennis D.C., cv. In-
novatsiia (BCBNI), B. campestris f. biennis D.C. ×
× B. rapa L. × B. napus f. biennis D.C. (BCBRBN),
B. juncea (L.) Czern. (BJ), B. juncea (L.) Czern.
f. biennis, cv. Annushka (BJA), B. napus f. biennis
D.C., f. EROF-5 (BNE-5), B. rapa L. (BR), B. rapa
subsp. rapifera Metzger (BRR), Raphanus sativus L.
var. oleiformis Pers. (RSO), Miscanthus sac chariflorus
(Maxim.) Benth., cv. Snihopad (MSS), M. sinensis
Anderss. f. ESBMK-6 (MSE-6), Panicum virga-
tum L. f. PB (PVPB), P. virgatum L. f. PL (PVPL),
Sorghum bicolor (L.) Moench. f. ETSSDF-11 (SBE-11),
S. nigrum Roemer & Schultes f. ETSSCHF-1
(SNE-1), S. saccharatum (L.) Moench. cv. Medo ve
(SSM), S. sudanense (Piper) Stapf (SS), S. tech ni-
cum (Koern.) Bait. et Trab. (ST).
Plant raw material collected from the experi-
mental collections of Cultural Flora department of
M.M. Gryshko National Botanical Garden of the
NAS of Ukraine. All biochemical analyses were
conducted using the above-ground part of plants in
the stage of flowering (Brassicaceae) and seed rip-
ening (Poaceae). To determine antioxidant activity
of plants was investigated dried above-ground part
of plants. Antioxidant capacity of the methanolic,
ethanolic and aqueous extracts was determined ac-
cording to Brand-Williams et al. (1995) against
DPPH radical (2.2-diphenyl-1-picrylhydrazyl)
[15]. This method based on reaction of radical dis-
coloration. The procedure of determination of op-
tical density measured with 2800 UV/VIS Spec-
trophotometer, UNICO at wavelength 515 nm.
Dry mass (1 g) of investigated plants mixed with
25 ml of solvent. Extraction was carried out with
methanol and water during 12 hours with constant
stirring on shaker. 0.1 ml of antioxidant solution
was added to 3.9 ml of methanol DPPH· solution
(25 mg of radical per 100 ml of methanol with fur-
ther delution). Absorbance of radical solution was
in range 0.700—0.800. Optical density of the solu-
tion was measured after adding sample immedi-
ately and after 10 min of incubation in the dark.
Obtained results were calculated in percentage by
using formula: (A
0
—A
10
)/A
0
•100 (A
0
— absorb-
ance of the control solution (containing only
DPPH•); A
10
— absorbance in the presence of the
plant extract in DPPH• solution).
The content of ascorbic acid determined ac-
cording to V.P. Krishchenko [2]. The concentra-
tion of ascorbic acid (AA) of the acid extracts
determined by a 2.6-dichlorophenol-indophenol
method that based on the reduction properties of
AA. The content of carotene determined accord-
ing to B.P. Pleshkov [3]. The procedure carried out
in petrol extracts by spectrophotometric method
89ISSN 1605-6574. Інтродукція рослин, 2018, № 1
Antioxidant potential of some plants of Brassicaceae Burnett and Poaceae Barnhart.
using 2800 UV/VIS Spectrophotometer, Unico.
Mixtures were left in a shaker for 2 hours and their
absorbance was measured at the wave length of 440
nm. Mean values of three replicates and standard
deviations are given in Table 1-4. Experimental
data were evaluated by using Excel 2010.
Results and discussions
The genus Brassica L. is the most important one
within the Brassicaceae, which includes some crops
and species of great worldwide economic im-
portance and medical such as Brassica juncea L.,
B. ole racea L., B. napus L. and B. rapa L. [8, 20,
23]. Brassica foods are very nutritive, providing nu-
trients and health-promoting phytochemicals such
Table 1. The total antioxidant activity of some crop plants of Brassicaceae Burnett.
Species, forms and variety Short name
% of inhibition of
methanol extract water extract
Brassica campestris f. annua D.C. BCA 16.94 ± 0.15 37.41 ± 0.50
Brassica campestris f. biennis D.C.× B. napus f.
biennis D.C., cv. Innovatsiia BCBNI 21.54 ± 0.22 50.62 ± 0.43
Brassica campestris f. biennis D.C. × B. rapa L. ×
× B. napus f. biennis D.C. BCBRBN 25.01 ± 0.49 65.66 ± 0.20
B. juncea (L.) Czern. BJ 19.16 ± 0.17 26.53 ± 0.34
B. juncea (L.) Czern. f. biennis, cv. Annushka BJA 19.79 ± 0.05 54.19 ± 0.49
B. napus f. biennis D.C., f. EROF-5 BNE-5 30.83 ± 0.31 60.79 ± 0.69
B. rapa L. BR 19.86 ± 0.29 62.96 ± 0.43
B. rapa subsp. rapifera Metzger BRR 36.91 ± 0.26 60.63 ± 0.55
Raphanus sativus L. var. oleiformis Pers. RSO 21.01 ± 0.23 65.85 ± 0.30
as vitamins, carotenoids, fiber, soluble sugars, min-
erals, glucosinolates and phenolic compounds [26,
29]. Cruciferous vegetables are relatively abundant
sources of antioxidant substances with potential
anticarcinogenic and antimicrobial activity [6, 14,
16, 17, 18, 30].
As shown in Table 1 the methanol extracts of
investigated plants of Brassicaceae have antioxi-
dant ability from 16.94 (BCA) to 36.91 (BRR) %.
We also measured the antioxidant activity of water
extracts. Results of investigation showed that wa-
ter extracts of these plants inhibited radical solu-
tion in range from 26.53 (BJ) to 65.85 (RSO) %.
As reported Kucukboyaci et al. (2012), species
of genus Raphanus is good candidates for a rich
Table 2. The total antioxidant activity of some crop plants of Poaceae Barnhart.
Species, forms and variety Short name
% of inhibition of
methanol extract water extract
Miscanthus sacchariflorus (Maxim.) Benth.,
cv. Snihopad MSS 39.43 ± 0.46 59.09 ± 0.11
M. sinensis Anderss., f. ESBMK-6 MSE-6 31.13 ± 0.32 45.38 ± 0.22
Panicum virgatum L., f. PB PVPB 40.81 ± 0.08 83.14 ± 0.46
P. virgatum L. f. PL PVPL 35.80 ± 0.31 40.16 ± 0.45
Sorghum bicolor (L.) Moench, f. ETSSDF-11 SBE-11 64.31 ± 0.27 57.37 ± 0.47
S. nigrum Roemer & Schultes, f. ETSSCHF-1 SNE-1 86.48 ± 0.49 64.13 ± 0.54
S. saccharatum (L.) Moench, cv. Medove SSM 31.34 ± 0.27 39.40 ± 0.28
Sorghum sudanense (Piper) Stapf SS 74.11 ± 0.54 42.24 ± 0.42
S. technicum (Koern.) Bait. et Trab ST 34.44 ± 0.20 33.10 ± 0.47
90 ISSN 1605-6574. Інтродукція рослин, 2018, № 1
O.M. Vergun, D.B. Rakhmetov
source of natural antioxidants and minerals [10].
As reported Borc et al. (2015), the same species
has an antioxidant capacity of 12.0—75.0 % de-
pending on variety [33]. According to Agarwal and
Varma (2014), aqueous extracts of plants of Rap-
hanus sativus have a potent antioxidant ability of
78.17 % [7]. Some results showed the radical inhi-
bition of extracts of these plants of 18.70 % [22].
When comparing these results with those obtained
from given literature in the same assay for B. rapa,
it could be noticed that plant extracts of turnip ex-
hibits high antioxidant capacity up to 72 % [27].
Also, Beltagi (2014) obtained data that different
organs extracted by different solvents showed the
antiradical activity by DPPH-method in range
from 11.11 to 84.75 % [13]. Our previous data
concerning antioxidant activity of representatives
of Brassicaceae showed that plant raw material of
Camelina sativa (L.) Crantz has antioxidant scav-
enging ability of 25.67—55.88 % in methanol ex-
tracts and 47.18—84.60 % in water extracts [1].
Species of genus Miscanthus, Panicum and Sor-
ghum are economically, energetically and ecologi-
cally viable energy crop. These plants have high
biomass productivity, high input use efficiency,
broad geographic adaptability, low environmental
risk, and low production cost [25, 31].
Our results showed that methanol extracts of
plants of Poaceae family were in range from 31.13
(MSE-6) to 86.48 (SNE-1) % (Table 2). Water ex-
tracts showed percentage of inhibition from 33.10
(ST) to 83.14 (PVPB) %.
As reported Balcerek et al. (2009), methanol
extracts of M. sinensis characterized by radical
scavenging activity of 27.00 % [9]. In our experi-
ment it was by registered more than 4 %. Devi et
Table 3. The total content of ascorbic acid and carotene in above-ground part of plants of Brassicaceae Burnett.
Species, forms and variety Short name Ascorbic acid, mg% Carotene, mg%
Brassica campestris f. annua D.C. BCA 353.86 ± 14.75 1.77 ± 0.03
Brassica campestris f. biennis D.C.× B. napus
f. biennis D.C., cv. Innovatsiia BCBNI 549.56 ± 33.81 1.93 ± 0.07
Brassica campestris f. biennis D.C. × B. rapa L. ×
× B. napus f. biennis D.C. BCBRBN 383.97 ± 27.33 2.73 ± 0.11
B. juncea (L.) Czern. BJ 563.51 ± 28.33 3.11 ± 0.07
B. juncea (L.) Czern. f. biennis, cv. Annushka BJA 118.67 ± 9.90 0.48 ± 0.01
B. napus f. biennis D.C., f. EROF-5 BNE-5 566.61 ± 38.37 2.74 ± 0.09
B. rapa L. BR 460.96 ± 5.76 1.73 ± 0.27
B. rapa subsp. rapifera Metzger BRR 290.09 ± 17.23 2.47 ± 0.11
Raphanus sativus L. var. oleiformis Pers. RSO 187.07 ± 14.27 3.05 ± 0.21
Table 4. The total content of ascorbic acid and carotene in above-ground part of plants of Poaceae Barnhart.
Species, forms and variety Short name Ascorbic acid, mg% Carotene, mg%
Miscanthus sacchariflorus (Maxim.) Benth.,
cv. Snihopad MSS 13.64 ± 0.72 0.16 ± 0.02
M. sinensis Anderss., f. ESBMK-6 MSE-6 17.16 ± 1.24 0.17 ± 0.01
Panicum virgatum L., f. PB PVPB 11.80 ± 0.85 0.05 ± 0.00
P. virgatum L. f. PL PVPL 13.94 ± 1.27 0.62 ± 0.02
Sorghum bicolor (L.) Moench, f. ETSSDF-11 SBE-11 35.12 ± 0.77 0.31 ± 0.05
S. nigrum Roemer & Schultes, f. ETSSCHF-1 SNE-1 33.86 ± 1.26 0.33 ± 0.05
S. saccharatum (L.) Moench, cv. Medove SSM 22.98 ± 1.08 0.26 ± 0.02
S. sudanense (Piper) Stapf SS 77.39 ± 1.29 0.39 ± 0.08
S. technicum (Koern.) Bait. et Trab ST 26.63 ± 0.80 0.30 ± 0.06
91ISSN 1605-6574. Інтродукція рослин, 2018, № 1
Antioxidant potential of some plants of Brassicaceae Burnett and Poaceae Barnhart.
al. (2012) identified that samples of red sorghum
bran extracted in acidified methanol had signifi-
cantly higher antioxidant activity than those ex-
tracted in methanol. This implies that the acidi-
fied methanol is a more powerful solvent than
methanol in extracting red sorghum antioxidants
(scavenging effect was up to 95.0 %) [19].
Vitamin A and C are the popular antioxidants,
which play a crucial role in preventing peroxida-
tion damage in the biological system [21]. Ascorbic
acid is an antioxidant with therapeutic properties,
which plays a key role in activating the immune
response, wound healing, osteogenesis, detoxify-
ing the organism, iron absorption, collagen bio-
synthesis, preventing the clotting of blood vessels,
and many other metabolic processes [28].
The results demonstrated the accumulation of
ascorbic acid in Brassicaceae plants in range from
118.67 (BJA) to 566.61 (BNE-5) mg% (Table 3).
Some results showed that content of vitamin C
(measured as ascorbic acid and dehydroascorbic
acid) of Brassicaceae leaves ranges among 64—104
mg per 100 g per fresh weight [5]. According to
Singh et al. (2007), investigated Brassica vegeta-
bles have maximum mean of ascorbic acid content
of 52.9 mg%, our samples showed higher results
[34]. Acikgoz (2016) detected the content of as-
corbic acid in Brassica rapa L. subsp. chinensis L.
was 44.27 mg% in late autumn period [4].
Carotenoids such as α- and β-carotene present
in dark green leafy vegetables of Brassicaceae
might be involved in the prevention of several dis-
eases related to oxidative stress [11]. Carotenoids
are efficient antioxidants protecting plants against
oxidative damage. Vitamin C, for instance, the most
powerful water-soluble antioxidant in human blood
plasma acts as regenerator for vitamin E in lipid
system [32].
We determined that content of total carotene in
above-ground part of investigated plants was from
0.48 (BJA) to 3.11 (BJ) mg% (Table 3). As report-
ed Singh et al. (2007), it was found content of
carotene from 0.01 to 0.81 mg% for different Bras-
sica vegetables [34].
The content of vitamins in Miscanthus, Pani-
cum and Sorghum species were analyzed (Table 4).
The represented data revealed that concentration
of ascorbic acid ranged from 11.80 (PVPB) to
77.39 (SS) mg%. Content of carotene in investi-
gated samples wasn’t high but ranged from 0.05
(PVPB) to 0.62 (PVPL) mg%.
Conclusions
From the results of this study it can be concluded
that plants from families Brassicaceae and Poaceae
are important source of antioxidants and vitamins
such as ascorbic acid and carotene. Experiment
showed that methanol and aqueous extracts of
plants of Poaceae (up to 80 %) had significantly
higher antiradical activity then the one of Brassi-
caceae (up to 35 % and 65 % respectively). Better
result of antioxidant capacity showed plant ex-
tracts of BRR and SNE-1 in methanol solvent and
RSO and PVPB — in aqueous. It should be noted
that for plants BJA (Brassicaceae) and PVPB (Po-
aceae) the content of ascorbic acid and carotene
was minimal.
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93ISSN 1605-6574. Інтродукція рослин, 2018, № 1
Antioxidant potential of some plants of Brassicaceae Burnett and Poaceae Barnhart.
33. Total phenolic content and antioxidant capacity of
Radish as influenced by the variety and vegetative
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Bulletin UASVM Food Science and Technology. —
2015. — Vol. 72, N 1. — P. 77—81. doi 10.15835/
buasvmcn-fst:11087
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ics and Genomics: Crops and Models. — New York:
Springer, 2011. — P. 33—65. — http://dx.doi.org/10.1007/
978-1-4419-7118-0_2
Рекомендувала Н.А. Павлюченко
Надійшла 01.12.2017
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bicolor) bran. Biotechnology Research International,
Article ID 258787. doi:10.1155/2012/258787
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(2011), Hepatoprotective effects from the leaf extracts
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94 ISSN 1605-6574. Інтродукція рослин, 2018, № 1
O.M. Vergun, D.B. Rakhmetov
22. Kim, J.K., Baskar, T.B. and Park, S.U. (2016), Total
phenolic and flavonoid contents and antioxidant ac-
tivities of two Raphanus sativus L. cultivars (Cherry
Belle and Valentine). Biosciences Biotechnology Re-
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org/10.13005/bbra/1999
23. Kwak, Y., Lee, J. and Ju, J. (2016), Anti-cancer activi-
ties of Brassica juncea leaves in vitro. EXCLI Journal,
vol.15, pp. 699—710. http://dx.doi.org/10.17179/ex-
cli2016-586
24. Marinova, G. and Batchvarov, V. (2011), Evaluation of
the methods for determination of the free radical scav-
enging activity by DPPH. Bulgarian Journal of Agri-
cultural Science, vol. 17, N 1, pp. 11—24.
25. Brosse, N., Dufour, A., Meng, X., Sun, Q. and Ragaus-
kas, A. (2012), Miscanthus: a fast-growing crop for bio-
fuels and chemicals production. Biofuels, Bioproducts
and Biorefening. doi: 10.1002/bbb
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dant potential and nutritional values of vegetables: a
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Ali, S., Zia, A. and Ali, S. (2012), Nutritional facts and
free radical scavenging activity of turnip (Brassica
rapa) from Pakistan. World Applied Sciences Journal,
vol. 19, N 3, pp. 370—375. doi: 10.5829/idosi.wasj.
2012.19.03.691
28. Pisoschi, A.M. and Negulescu, G.P. (2011), Methods
for Total Antioxidant Activity Determination: A Re-
view. Biochemistry and Analytical Biochemistry, vol. 1,
N 1. doi:10.4172/2161-1009.1000106
29. Podsedek, A. (2007), Natural antioxidants and antioxi-
dant capacity of Brassica vegetables: a review. LWT —
Food Science and Technology, vol. 40, pp. 1—11.
30. Prasad, M.P. (2014), Antimicrobial potential of Brassi-
caceae family against clinical isolates. International
Journal of Pure and Applied Bioscience, vol. 2, N 2,
pp. 158—162.
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niry, J.R. (2012), Soil and variety effects on the energy
and carbon balances of Switchgrass-derived ethanol.
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ca rotenoids. Molecular Aspects of Medicine, vol. 24,
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dovan, O., Vlaic, R. and Tofana, M. (2015), Total phe-
nolic content and antioxidant capacity of Radish as
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Rai, M. (2007), Variability of carotenes, vitamin C, E
and phenolics in Brassica vegetables. Journal of Food
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Genetics and Genomics of the Brassicaceae. Plant
Genetics and Genomics: Crops and Models, New
York: Springer, pp. 33—65. http://dx.doi.org/10.1007/
978-1-4419-7118-0_2
Recommended by N.A. Pavlyuchenko
Received 01.12.2017
О.М. Вергун, Д.Б. Рахметов
Національний ботанічний сад
імені М.М. Гришка НАН України,
Україна, м. Київ
АНТИОКСИДАНТНИЙ ПОТЕНЦІАЛ
РОСЛИН РОДИН BRASSICACEAE BURNETT
ТА POACEAE BARNHART.
Мета — дослідити антирадикальну дію екстрактів де-
яких представників родин Brassicaceae Burnett та Poa-
ceae Barnhart. для оцінки загальної антиоксидантної
активності рослин в умовах Національного ботаніч-
ного саду імені М.М. Гришка НАН України.
Матеріал та методи. Рослинний матеріал дослі-
дження — види, форми та сорти рослин-представни-
ків Brassicaceae та Poaceae: Brassica campestris f. annua
D.C. (BCA), B. campestris f. biennis D.C.× B. napus f.
biennis D.C., cv. Innovatsiia (BCBNI), B. campestris f.
biennis D.C. × B. rapa L. × B. napus f. biennis D.C. (BCBRBN),
B. juncea (L.) Czern. (BJ), B. juncea (L.) Czern. f. biennis,
cv. Annushka (BJA), B. napus f. biennis D.C., f. EROF-5
(BNE-5), B. rapa L. (BR), B. rapa subsp. rapifera Metzger
(BRR), Raphanus sativus L. var. oleiformis Pers. (RSO),
Miscanthus sacchariflorus (Maxim.) Benth., cv. Snihopad
(MSS), M. sinensis Anderss. f. ESBMK-6 (MSE-6), Pani-
cum virgatum L. f. PB (PVPB), P. virgatum L. f. PL (PVPL),
Sorghum bicolor (L.) Moench. f. ETSSDF-11 (SBE-11),
S. nigrum Roemer & Schultes f. ETSSCHF-1 (SNE-1), S.
saccharatum (L.) Moench. cv. Medove (SSM), S. sudanense
(Piper) Stapf (SS), S. technicum (Koern.) Bait. et Trab. (ST).
Антиоксидантну активність метанольних та водних екс-
трактів, в основі якої лежить реакція знебарвлення
розчину ДФПГ (2,2-дифеніл-1-пікрилгідразил-віль-
ний радикал), визначали за Brandt-Williams et al., вміст
аскорбінової кислоти — за В.П. Крищенком, кароти-
ну — за Б.П. Плешковим.
Результати. Антиоксидантна активність метаноль них
екстрактів рослин родини Brassicaceae становила від
16,94 ± 0,15 (BCA) до 36,91 ± 0,26 (BRR) %, водних —
від 26,53 ± 0,34 (BJ) до 65,85 ± 0,30 (RSO) %, метаноль-
них екстрактів рослин родини Poaceae — від 31,13 ±
0,32 (MSE-6) до 86,48 ± 0,49 (SNE-1) %, водних — від
33,10 ± 0,47 (ST) до 83,14 ± 0,46 (PVPB) %, концентра-
ція аскорбінової кислоти у рослин родини Brassica-
ceae — від 118,67 ± 9,90 (BJA) до 566,61 ± 38,37 (BNE-5) мг%,
95ISSN 1605-6574. Інтродукція рослин, 2018, № 1
Antioxidant potential of some plants of Brassicaceae Burnett and Poaceae Barnhart.
каротину — від 0,48 ± 0,01 (BJA) до 3,11 ± 0,07 (BJ) мг%,
у рослин родини Poaceae — відповідно від 11,80 ±
± 0,85 (PVPB) до 77,39 ± 1,29 (SS) мг% та від 0,05 ±
± 0,00 (PVPB) до 0,62 ± 0,02 (PVPL) мг%.
Висновки. Рослинна сировина досліджених рос-
лин є цінним джерелом антиоксидантів і вітамінів.
Метанольні та водні екстракти рослин родини Poa-
ceae мали вищу антирадикальну активність щодо
ДФПГ-радикала, ніж екстракти рослин родини Bras-
sicaceae. Найвищий рівень аскорбінової кислоти та
каротину виявлено в екстрактах рослин родини Bras-
sicaceae, мінімальний — у BJA (Brassicaceae) та PVPB
(Poaceae).
Ключові слова: Brassicaceae, Poaceae, антиоксидантна
активність, 2,2-дифеніл-1-пікрилгідразил (ДФПГ), ас-
корбінова кислота, каротин.
Е.Н. Вергун, Д.Б. Рахметов
Национальный ботанический сад
имени Н.Н. Гришко НАН Украины,
Украина, г. Киев
АНТИОКСИДАНТНЫЙ ПОТЕНЦИАЛ
РАСТЕНИЙ СЕМЕЙСТВ BRASSICACEAE
BURNETT И POACEAE BARNHART.
Цель — исследовать антирадикальное действие экс-
трактов некоторых представителей семейств Bras si ca-
ceae Burnett и Poaceae Barnhart. для оценки общей анти-
оксидантной активности в условиях Национального бо-
танического сада имени Н.Н. Гришко НАН Украины.
Материал и методы. Растительный материал иссле-
дования — виды, формы и сорта растений семейств
Brassicaceae и Poaceae: Brassica campestris f. annua D.C.
(BCA), B. campestris f. biennis D.C.× B. napus f. biennis
D.C., cv. Innovatsiia (BCBNI), B. campestris f. biennis
D.C. × B. rapa L. × B. napus f. biennis D.C. (BCBRBN),
B. juncea (L.) Czern. (BJ), B. juncea (L.) Czern. f. biennis,
cv. Annushka (BJA), B. napus f. biennis D.C., f. EROF-5
(BNE-5), B. rapa L. (BR), B. rapa subsp. rapifera Metzger
(BRR), Raphanus sativus L. var. oleiformis Pers. (RSO),
Miscanthus sacchariflorus (Maxim.) Benth., cv. Snihopad
(MSS), M. sinensis Anderss. f. ESBMK-6 (MSE-6), Pani-
cum virgatum L. f. PB (PVPB), P. virgatum L. f. PL (PVPL),
Sorghum bicolor (L.) Moench. f. ETSSDF-11 (SBE-11),
S. nigrum Roemer & Schultes f. ETSSCHF-1 (SNE-1), S. sac-
charatum (L.) Moench. cv. Medove (SSM), S. sudanense
(Piper) Stapf (SS), S. technicum (Koern.) Bait. et Trab.
(ST). Антиоксидантную активность метанольных и
водных экстрактов, основанную на реакции обесцве-
чивания раствора ДФПГ (2,2-дифенил-1-пикрил гидра-
зил-свободный радикал), определяли по Brandt-Williams
et al., содержание ас корбиновой кислоты — В.П. Кри-
щенко, каротина — по Б.П. Плешкову.
Результаты. Антиоксидантная активность метаноль-
ных экстрактов растений семейства Brassicaceae со-
ставляла от 16,94 ± 0,15 (BCA) до 36,91 ± 0,26 (BRR) %,
водных — от 26,53 ± 0,34 (BJ) до 65,85 ± 0,30 (RSO) %,
метанольных экстрактов растений семейства Poaceae —
от 31,13 ± 0,32 (MSE-6) до 86,48 ± 0,49 (SNE-1) %, вод-
ных — от 33,10 ± 0,47 (ST) до 83,14 ± 0,46 (PVPB) %,
концентрация аскорбиновой кислоты у растений се-
мейства Brassicaceae — от 118,67±9,90 (BJA) до 566,61 ±
± 38,37 (BNE-5) мг%, каротина — от 0,48 ± 0,01 (BJA)
до 3,11 ± 0,07 (BJ) мг%, у растений семейства Poaceae —
соответственно от 11,80 ± 0,85 (PVPB) до 77,39 ±
± 1,29 (SS) мг% и от 0,05 ± 0,00 (PVPB) до 0,62 ±
± 0,02 (PVPL) мг%.
Выводы. Растительное сырье исследованных рас-
тений является ценным источником антиоксидантов
и витаминов. Метанольные и водные экстракты рас-
тений семейства Poaceae имели большую антиради-
кальную активность относительно ДФПГ-радикала,
чем экстракты растений семейства Brassicaceae. Наи-
высший уровень аскорбиновой кислоты и каротина
выявлен в экстрактах растений семейства Brassicaceae,
минимальный — у BJA (Brassicaceae) и PVPB (Poaceae).
Ключевые слова: Brassicaceae, Poaceae, антиоксидантная
активность, 2,2-дифенил-1-пикрилгидразил (ДФПГ),
аскорбиновая кислота, каротин.
|
| id | oai:ojs2.plantintroduction.org:article-40 |
| institution | Plant Introduction |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2025-07-17T12:39:07Z |
| publishDate | 2018 |
| publisher | M.M. Gryshko National Botanical Garden of the NAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | wwwplantintroductionorg/9d/fd791cc08d7b553dfd99effe433f029d.pdf |
| spelling | oai:ojs2.plantintroduction.org:article-402019-11-11T08:15:00Z Antioxidant potential of some plants of Brassicaceae Burnett and Poaceae Barnhart Антиоксидантний потенціал рослин родин Brassicaceae Burnett та Poaceae Barnhart. Vergun, O.M. Rakhmetov, D.B. Objective – to estimate the antiradical scavenging ability of extracts of some plants of Brassicaceae Burnett and Poaceae Barnhart. species, to evaluate the total antioxidant activity of plants in the conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. Plant material of this investigation – species, varieties and cultivars of Brassicaceaae and Poaceae families: Brassica campestris f. annua D.C. (BCA), B. campestris f. biennis D.C.× B. napus f. biennis D.C., cv. Innovatsiia (BCBNI), B. campestris f. biennis D.C. × B. rapa L. × B. napus f. biennis D.C. (BCBRBN), B. juncea (L.) Czern. (BJ), B. juncea (L.) Czern. f. biennis, cv. Annushka (BJA), B. napus f. biennis D.C., f. EROF-5 (BNE-5), B. rapa L. (BR), B. rapa subsp. rapifera Metzger (BRR), Raphanus sativus L. var. oleiformis Pers. (RSO), Miscanthus sacchariflorus (Maxim.) Benth., cv. Snihopad (MSS), M. sinensis Anderss. f. ESBMK-6 (MSE-6), Panicum virgatum L. f. PB (PVPB), P. virgatum L. f. PL (PVPL), Sorghum bicolor (L.) Moench. f. ETSSDF-11 (SBE-11), S. nigrum Roemer et Schultes f. ETSSCHF-1 (SNE-1), S. saccharatum (L.) Moench. cv. Medove (SSM), S. sudanense (Piper) Stapf (SS), S. technicum (Koern.) Bait. et Trab. (ST). The antioxidant activity of methanol and aqueous extracts based on the discoloration reaction on the solution of DPPH (2,2-diphenyl-1-picrylhydrazyl free radical) was determined according to Brandt-Williams et al. The determination of content of ascorbic acid was conducted according to V.P. Krishchenko, the content of carotene – according to B.P. Pleshkov. Results. The antioxidant activity of methanol extracts of plants of Brassicaceae was registered in the range from 16.94±0.15 (BCA) to 36.91 ± 0.26 (BRR) % and aqueous extracts – from 26.53 ± 0.34 (BJ) to 65.85 ± 0.30 (RSO) %. This parameter for methanol extracts of Poaceae species was in range from 31.13 ± 0.32 (MSE-6) to 86.48 ± 0.49 (SNE-1) % and aqueous extracts – from 33.10 ± 0.47 (ST) to 83.14 ± 0.46 (PVPB) %. It was determined concentration of ascorbic acid from 118.67 ± 9.90 (BJA) to 566.61 ± 38.37 (BNE-5) mg% and carotene – from 0.48 ± 0.01 (BJA) to 3.11 ± 0.07 (BJ) mg% for Brassicaceae plants. Content of ascorbic acid in range from 11.80 ± 0.85 (PVPB) to 77.39 ± 1.29 (SS) mg% and carotene – from 0.05 ± 0.00 (PVPB) to 0.62 ± 0.02 (PVPL) mg% was identified for Poaceae plants. Conclusions. This study showed that plant raw material of investigated plants is a valuable source of antioxidants and vitamins. Methanol and water extracts of plants of Poaceae had higher antiradical activity against DPPH radical than extracts of plants of Brassicaceae. The higher level of ascorbic acid and carotene was observed in extracts of plants of Brassicaceae. The minimal content of ascorbic acid and carotene found for BJA (Brassicaceae) and PVPB (Poaceae). Мета – дослідити антирадикальну дію екстрактів деяких представників родин Brassicaceae Burnett та Poaceae Barnhart. для оцінки загальної антиоксидантної активності рослин в умовах Національного ботанічного саду імені М.М. Гришка НАН України. Матеріал та методи. Рослинний матеріал дослідження – види, форми та сорти рослин-представни-ків Brassicaceae та Poaceae: Brassica campestris f. annua DC. (BCA), B. campestris f. biennis DC. x B. napus f. biennis DC., cv. Innovatsiia (BCBNI), B. campestris f. biennis DC. x B. rapa L. x B. napus f. biennis DC. (BCBRBN), B. juncea (L.) Czern. (BJ), B. juncea (L.) Czern. f. biennis, cv. Annushka (BJA), B. napus f. biennis D.C., f. EROF-5 (BNE-5), B. rapa L. (BR), B. rapa subsp. rapifera Metzger (BRR), Raphanus sativus L. var. oleiformis Pers. (RSO), Miscanthus sacchariflorus (Maxim.) Benth., cv. Snihopad (MSS), M. sinensis Anderss. f. ESBMK-6 (MSE-6), Panicum virgatum L. f. PB (PVPB), P. virgatum L. f. PL (PVPL), Sorghum bicolor (L.) Moench. f. ETSSDF-11 (SBE-11), S. nigrum Roemer et Schultes f. ETSSCHF-1 (SNE-1), S. saccharatum (L.) Moench. cv. Medove (SSM), S. sudanense (Piper) Stapf (SS), S. technicum (Koern.) Bait. et Trab. (ST). Антиоксидантну активність метанольних та водних екстрактів, в основі якої лежить реакція знебарвлення розчину ДФПГ (2,2-дифеніл-1-ткрилгідразил-вільний радикал), визначали за Brandt-Williams et al., вміст аскорбінової кислоти – за В.П. Крищенком, каротину – за Б.П. Плешковим. Результати. Антиоксидантна активність метанольних екстрактів рослин родини Brassicaceae становила від 16,94 ± 0,15 (BCA) до 36,91 ± 0,26 (BRR) %, водних – від 26,53 ± 0,34 (BJ) до 65,85 ± 0,30 (RSO) %, метанольних екстрактів рослин родини Poaceae – від 31,13 ± 0,32 (MSE-6) до 86,48 ± 0,49 (SNE-1) %, водних – від 33,10 ± 0,47 (ST) до 83,14 ± 0,46 (PVPB) %, концентрація аскорбінової кислоти у рослин родини Brassicaceae – від 118,67 ± 9,90 (BJA) до 566,61 ± 38,37 (BNE-5) мг%, каротину – від 0,48 ± 0,01 (BJA) до 3,11 ± 0,07 (BJ) мг%, у рослин родини Poaceae – відповідно від 11,80 ± 0,85 (PVPB) до 77,39 ± 1,29 (SS) мг% та від 0,05 ± 0,00 (PVPB) до 0,62 ± 0,02 (PVPL) мг%. Висновки. Рослинна сировина досліджених рослин є цінним джерелом антиоксидантів і вітамінів. Метанольні та водні екстракти рослин родини Poaceae мали вищу антирадикальну активність щодо ДФПГ-радикала, ніж екстракти рослин родини Brassicaceae. Найвищий рівень аскорбінової кислоти та каротину виявлено в екстрактах рослин родини Brassicaceae, мінімальний – у BJA (Brassicaceae) та PVPB (Poaceae). M.M. Gryshko National Botanical Garden of the NAS of Ukraine 2018-03-01 Article Article application/pdf https://www.plantintroduction.org/index.php/pi/article/view/40 10.5281/zenodo.2174708 Plant Introduction; Vol 77 (2018); 87-95 Інтродукція Рослин; Том 77 (2018); 87-95 2663-290X 1605-6574 10.5281/zenodo.3377686 en https://www.plantintroduction.org/index.php/pi/article/view/40/34 Copyright (c) 2018 The Author(s) http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Vergun, O.M. Rakhmetov, D.B. Антиоксидантний потенціал рослин родин Brassicaceae Burnett та Poaceae Barnhart. |
| title | Антиоксидантний потенціал рослин родин Brassicaceae Burnett та Poaceae Barnhart. |
| title_alt | Antioxidant potential of some plants of Brassicaceae Burnett and Poaceae Barnhart |
| title_full | Антиоксидантний потенціал рослин родин Brassicaceae Burnett та Poaceae Barnhart. |
| title_fullStr | Антиоксидантний потенціал рослин родин Brassicaceae Burnett та Poaceae Barnhart. |
| title_full_unstemmed | Антиоксидантний потенціал рослин родин Brassicaceae Burnett та Poaceae Barnhart. |
| title_short | Антиоксидантний потенціал рослин родин Brassicaceae Burnett та Poaceae Barnhart. |
| title_sort | антиоксидантний потенціал рослин родин brassicaceae burnett та poaceae barnhart. |
| url | https://www.plantintroduction.org/index.php/pi/article/view/40 |
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