Антиоксидантна активність екстрактів насіння деяких кормових рослин
Objective – to investigate the antioxidant activity of seed extracts of selected forage plants in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. Seeds of seven forage plants researched in this study: Baptisia australis (L.) R. Br. (Fabaceae), Bunias...
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| Дата: | 2019 |
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M.M. Gryshko National Botanical Garden of the NAS of Ukraine
2019
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Репозитарії
Plant Introduction| _version_ | 1860144515482910720 |
|---|---|
| author | Vergun, O.M. Rakhmetov, D.B. Shymanska, O.V. Rakhmetova, S.O. Fishchenko, V.V. |
| author_facet | Vergun, O.M. Rakhmetov, D.B. Shymanska, O.V. Rakhmetova, S.O. Fishchenko, V.V. |
| author_sort | Vergun, O.M. |
| baseUrl_str | https://www.plantintroduction.org/index.php/pi/oai |
| collection | OJS |
| datestamp_date | 2019-11-11T08:15:58Z |
| description | Objective – to investigate the antioxidant activity of seed extracts of selected forage plants in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine.
Material and methods. Seeds of seven forage plants researched in this study: Baptisia australis (L.) R. Br. (Fabaceae), Bunias erucago L., B. orientalis L. (Brassicaceae), Galega officinalis L., G. orientalis Lam. (Fabaceae), Isatis littoralis Steven, I. tinctoria L. (Brassicaceae). The method described by Brand-Williams et al. (1995) used to determine the antiradical activity of plant extracts. Biochemical analyze included following stages: preparation of methanol, ethanol and water extracts (1 g of dried material mixed with 25 ml of solvent); 12 hours of extraction; spectrophotometric procedure of determination of antiradical activity with a 2,2-diphenyl-2-picrylhydrazyl solution. A solution of radical prepared in methanol and diluted according to method. Data were expressed in ascorbic acid equivalent (AAE) and Trolox equivalent (TE) on a gram of dry weight. Measurements of extracts carried out on spectrophotometer UNICO UV 2800 at 515 nm. Experimental data processed by Excel.
Results. Methanol extracts of investigated plants had an antiradical activity of 18.27–80.57 %, ethanol extracts of 11.07–79.73 % and water extracts of 23.31–80.26 %. Antioxidant activity of methanol extracts expressed on ascorbic acid equivalent was 1.75–2.72 mg AAE per gram, ethanol extracts 1.62–2.72 mg AAE per gram and water extracts 1.82–2.66 mg AAE per gram. Results exhibited antioxidant activity expressed on Trolox equivalent of methanol extracts from 1.30 to 7.51 mg TE per gram, ethanol extracts from 0.56 to 7.76 mg TE per gram and water extracts from 1.19 to 7.37 mg TE per gram.
Conclusions. Obtained data demonstrated that in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine seed extracts of investigated forage plants have high antioxidant potential as well as leaves or shoots. Antiradical activity of extracts was of 11.07–84.60 %. All seed extracts had the least values of this parameter in the ethanolic extracts. Antioxidant activity expressed in ascorbic acid equivalent was maximal for Bunias orientalis and Galega officinalis and minimal for Isatis tinctoria. Antioxidant activity by Trolox equivalent was high for Bunias orientalis methanol extracts and slow for Bunias erucago ethanol extracts. Investigation of seeds extracts of crops from different plant families is need to evaluate antioxidant potential of it and recommend for use. |
| doi_str_mv | 10.5281/zenodo.3241204 |
| first_indexed | 2025-07-17T12:48:12Z |
| format | Article |
| fulltext |
71ISSN 16056574. Інтродукція рослин, 2019, № 2: 71—76
https://doi.org/10.5281/zenodo.3241204
UDC 581.48:581.192
O.M. VERGUN, D.B. RAKHMETOV, O.V. SHYMANSKA, S.O. RAKHMETOVA, V.V. FISHCHENKO
M.M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine
Ukraine, 01014 Kyiv, Timiryazevska str., 1
ANTIOXIDANT ACTIVITY OF SEED EXTRACTS
OF SELECTED FORAGE PLANTS
Objective — to investigate the antioxidant activity of seed extracts of selected forage plants in conditions of M.M. Gryshko Na
tional Botanical Garden of the NAS of Ukraine.
Material and methods. Seeds of seven forage plants researched in this study: Baptisia australis (L.) R.Br. (Fabaceae), Bu
nias erucago L., B. orientalis L. (Brassicaceae), Galega officinalis L., G. orientalis Lam. (Fabaceae), Isatis littoralis Steven,
I. tinctoria L. (Brassicaceae). The method described by BrandWilliams et al. (1995) used to determine the antiradical activ
ity of plant extracts. Biochemical analyze included following stages: preparation of methanol, ethanol and water extracts (1 g
of dried material mixed with 25 ml of solvent); 12 hours of extraction; spectrophotometric procedure of determination of anti
radical activity with a 2,2diphenyl2picrylhydrazyl solution. A solution of radical prepared in methanol and diluted according
to method. Data were expressed in ascorbic acid equivalent (AAE) and Trolox equivalent (TE) on a gram of dry weight. Meas
urements of extracts carried out on spectrophotometer UNICO UV 2800 at 515 nm. Experimental data processed by Excel.
Results. Methanol extracts of investigated plants had an antiradical activity of 18.27—80.57 %, ethanol extracts of 11.07—
79.73 % and water extracts of 23.31—80.26 %. Antioxidant activity of methanol extracts expressed on ascorbic acid equivalent
was 1.75—2.72 mg AAE per gram, ethanol extracts 1.62—2.72 mg AAE per gram and water extracts 1.82—2.66 mg AAE per
gram. Results exhibited antioxidant activity expressed on Trolox equivalent of methanol extracts from 1.30 to 7.51 mg TE per
gram, ethanol extracts from 0.56 to 7.76 mg TE per gram and water extracts from 1.19 to 7.37 mg TE per gram.
Conclusions. Obtained data demonstrated that in conditions of M.M. Gryshko National Botanical Garden of the NAS of
Ukraine seed extracts of investigated forage plants have high antioxidant potential as well as leaves or shoots. Antiradical activ
ity of extracts was of 11.07—84.60 %. All seed extracts had the least values of this parameter in the ethanolic extracts. Antioxi
dant activity expressed in ascorbic acid equivalent was maximal for Bunias orientalis and Galega officinalis and minimal for
Isatis tinctoria. Antioxidant activity by Trolox equivalent was high for Bunias orientalis methanol extracts and slow for Bunias
erucago ethanol extracts. Investigation of seeds extracts of crops from different plant families is need to evaluate antioxidant
potential of it and recommend for use.
Key words: seeds, alcoholic and water extracts, antiradical activity, antioxidant activity.
© O.M. VERGUN, D.B. RAKHMETOV, O.V. SHYMANSKA,
S.O. RAKHMETOVA, V.V. FISHCHENKO, 2019
The growing demand for natural antioxidants ex
ists in food and cosmetic industries and requires
the new sources of these compounds. Antioxidant
activity is an important and widely studied param
eter of plant raw material investigation. Last time
there are numerous reports about the accumula
tion of different antioxidants in different parts of
plant organism [6]. Plant antioxidants are known
as agents that reduce the risk of many chronic dis
eases and play an important role in plants [14].
According to Kumar et al. (2017), antioxidants
in clude two main types such as antioxidants based
on solubility (ascorbic acid, glutathione, lipoic
acid, uric acid etc.) and based on the occurrence
(vitamin A, vitamin C, vitamin E, betacarotene
etc.) [14]. Investigation of seed antioxidant capac
ity actual concerning to active oxygen species,
which, as reported Bailly (2004), may play a role
in desiccationrelated damage, particularly in de
hydrationintolerant recalcitrant seeds [2].
During seed sprouting a multitude of biochem
ical processes takes place, which caused radical
changes in biochemical composition. It can lead
to a change in phenolic compounds profile and
antioxidant activity [5]. Some results showed that
germinated seeds increased the polyphenol con
tent and relating to this antioxidant activity [13;
72 ISSN 16056574. Plant introduction, 2019, № 2
O.M. Vergun, D.B. Rakhmetov, O.V. Shymanska, S.O. Rakhmetova, V.V. Fishchenko
19]. But the study of some Fabaceae seeds demon
strated that total phenolic compounds decreased
during sprouting. Thus, accumulation antioxi
dants and linear relationship between polyphenol
compounds and antioxidant activity of seeds de
pends on plant species and, also, conditions of
germination [9; 16].
Investigation of forage plants still is an actual
branch of modern biology because of their use in
agriculture and, also, these plants are an impor
tant source of biologically active compounds. In
addition, these plants can be used as medicinal,
plant raw material of which exhibit different bio
logical activities such as microbiological, antioxi
dant, anticancer effect [8; 11; 12; 17].
Objective — to investigate the antioxidant ac
tivity of seed extracts of selected forage plants in
conditions of M.M. Gryshko National Botanical
Garden of the NAS of Ukraine.
Material and methods
Seeds were collected from the experimental collec
tion of Department of Cultural Flora in M.M. Grysh
ko National Botanical Garden of the NAS of
Ukraine (NBG) at the stage of full seed ripening. In
this study following species from a collection of for
age plants were tested: Baptisia australis (L.) R.Br.
(Fabaceae), Bunias erucago L., B. orientalis L. (Bras
sicaceae), Galega officinalis L., G. orientalis Lam. (Fa
baceae), Isatis littoralis Steven, I. tinctoria L. (Brassi
caceae).
Biochemical analyses were conducted in the
laboratory of Department Cultural Flora of NBG.
De termination of the antiradical activity of ex
tracts detected according to BrandWilliams [4].
Extracts preparation
For extraction, 1 g of dried and milled seeds
were mixed with 25 ml of solvent (methanol, eth
anol, and water). The procedure of extraction
continued for 12 hours at the constant shaking.
After this conducted filtration of obtained mix
tures.
DPPH radical scavenging activity
Obtained extracts were analyzed on antioxidant
capacity with DPPHradical (2,2diphenyl2pic
rylhydrazyl). A radical solution prepared by the
following procedure: 0.025 g of radical mixed with
100 ml of methanol. The obtained solution was
diluted for the next analyses (1:10). 3.9 ml of the
radical solution measured at the wavelength 515 nm
on the spectrophotometer UNICO UV 2800 and
added 100 µl of plant extract. After 10 minutes in
darkness obtained solution measured again at the
same wavelength. Results of measurements were
calculated by equation:
% Inh = ((A
0
– A
10
) : A
0
) · 100,
where % Inh — % of inhibition of radical solution;
A
0
— control measurement without plant extract;
A
10
— measurement with plant extract after 10 mi
nutes.
Obtained results also were expressed in ascorbic
acid equivalent (AAE) and Trolox equivalent (TE)
on a gram of dry weight.
Experimental data were evaluated by using Ex
cel 2010. Mean values of three replicates and
standard deviation are given in Figures 1, 2 and
Table.
Results and discussions
Investigation of forage plants plays an important
role in agricultural science. Plants from Fabaceae
such as Galega orientalis characterized by high
productivity and as Nfixators [18]. Bunias orien
talis that belongs to Brassiceae, besides value char
acteristics, demonstrated also antimicrobial activ
ity [21]. It is known, that plants of Isatis spp. ac
cumulate two indoxylforming substances in leav
es, which when exposed on air form indigo [10].
Our previous study of antioxidant activity showed
that different plants such as forage, energetic, oil
plants, medicinal etc. and it plants raw material
have the high antioxidant potential [20].
Various methods are used to identify the anti
oxidant property of plant raw material. Alam et al.
(2012) reviewed two basic groups of methods to
evaluate antioxidant properties of samples: in vitro
and in vivo methods. The first group of methods
includes DPPH scavenging activity, hydrogen per
oxide scavenging assay, nitric oxide scavenging ac
tivity, Trolox equivalent antioxidant capacity, total
radicaltrapping antioxidant parameter (TRAP)
method, ferric reducingantioxidant power, phos
phomolybdenum method etc. The second group of
methods usually use on animals such as mice, rats
73ISSN 16056574. Інтродукція рослин, 2019, № 2
Antioxidant activity of seed extracts of selected forage plants
etc. and their tissues can be used for the assay [1].
DPPH method is the most widespread and simple
assay based on the reaction of discoloration of the
radical solution [15].
Seed is a potential source of antioxidants [7]. In
our study, we used to investigate methanol, etha
nol and water extracts of seeds to determine DPPH
scavenging effect. On Fig. 1 demonstrated that
antiradical activity of seed extracts was the least
for ethanol extracts of all investigated plants. Anti
radical activity of methanol extracts of investigat
ed plants decreased in the following the order:
Ga lega officinalis > Bunias orientalis > Galega orien
talis > Bunias erucago > Baptisia australis > Isatis
littoralis > Isatis tinctoria. This parameter in etha
nol extracts decreased in following order: Bunias
orientalis > Galega orientalis > Galega officinalis >
Bunias erucago > Isatis littoralis > Baptisia australis >
Isatis tinctoria. Scavenging effect of seed extracts
against DPPH radical decreased in the following
the order: Bunias orientalis > Galega officinalis >
Ga lega orientalis > Baptisia australis > Bunias eru
cago > Isatis littoralis > Isatis tinctoria.
Generally, methanol extracts of investigated plants
had an antiradical activity of 18.27—80.57 %, etha
nol extracts of 11.07—79.73 % and water extracts of
23.31—80.26 %. Our previous investigation of anti
oxidant activity of Brassicaceae species demon
strated that plant raw material of aboveground part
of the plant has an antiradical activity of different
extracts from 25.67 to 84.60 %, wherein methanol
extracts had higher results [22].
As positive control during DPPH assay can be
used ascorbic acid, gallic acid, quercetin, rutin, cat
echin [14]. In our experiment chosen control com
pound the ascorbic acid equivalent (AAE). Gener
ally, methanol extracts showed antioxidant activity
from 1.75 to 2.72 mg AAE per gram, ethanol extracts
from 1.62 to 2.72 mg AAE per gram, water extracts
from 1.82 to 2.66 mg AAE per gram (Fig. 2).
%
90
80
70
60
50
40
30
20
10
0
Methanol extracts
Ethanol extracts
Water extracts
Baptisia
australis
Bunias
erucago
Bunias
orientalis
Galega
officinalis
Galega
orientalis
Isatis
littoralis
Isatis
tinctoria
3,0
2,5
2,0
1,5
1,0
0,5
0,0
Methanol extracts
Ethanol extracts
Water extracts
Baptisia
australis
Bunias
erucago
Bunias
orientalis
Galega
officinalis
Galega
orientalis
Isatis
littoralis
Isatis
tinctoria
Antioxidant activity of selected plant extracts, mg TE/g
Species Methanol extracts Ethanol extracts Water extracts
Baptisia australis 1.30 ± 0.13 — 3.27 ± 0.11
Bunias erucaga 6.74 ± 0.17 0.56 ± 0.04 1.96 ± 0.16
Bunias orientalis 7.51 ± 0.12 7.46 ± 0.05 7.37 ± 0.14
Galega officinalis 7.50 ± 0.39 4.00 ± 0.23 6.98 ± 0.21
Galega orientalis 7.28 ± 0.26 5.34 ± 0.31 5.68 ± 0.30
Isatis littoralis — — 1.19 ± 0.17
Isatis tinctoria — — —
Fig. 2. Antioxidant activity of seed extracts of selected
plant species, mg AAE/g
Fig. 1. Scavenging effect of seed extracts on DPPH radical, %
74 ISSN 16056574. Plant introduction, 2019, № 2
O.M. Vergun, D.B. Rakhmetov, O.V. Shymanska, S.O. Rakhmetova, V.V. Fishchenko
According to Borchardt et al. (2008), antioxi
dant activity from DPPH scavenging activity for
Baptisia australis was 12.99 µM Trolox per 100 g,
B. bracteata of 20.08 µM Trolox per 100 g, Lepi
dium virginicum 25.95 µM Trolox per 100 g, Cap
sella bursapastoris of 14.71 µM Trolox per 100 g
[3]. In our experiment methanol extracts exhibit
ed antioxidant activity from 1.30 to 7.51 mg Trolox
equivalent (TE) per gram, ethanol extracts from
0.56 to 7.46 mg TE per gram, water extracts from
1.19 to 7.37 mg TE per gram (Table). Investigated
concentrations of plant extracts of Isatis littoralis
(methanol, ethanol extracts), Isatis tinctoria (all
extracts) and Baptisia australis (ethanol extract)
couldn’t be expressed in Trolox equivalent.
Conclusions
This study indicates that seeds of investigated forage
plants have high antioxidant potential as well as leaves
or shoots. In conditions of M.M. Gryshko National
Botanical Garden of the NAS of Ukraine selected
seeds of forage plants had antiradical activity from
11.07 to 84.60 % depending on extract. All investi
gated seeds had the least values of this parameter in
the ethanolic extracts. Antioxidant activity expressed
in ascorbic acid equivalent (mg AAE/g) and Trolox
equivalent (mg TE/g) was of 1.62—2.72 and 0.56—
7.76 respectively. Investigation of seeds extracts of
crops from different plant families is need to evaluate
antioxidant potential of it and recommend for use.
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Recommended by O.V. Grygorieva
Received 31.01.2019
О.М. Вергун, Д.Б. Рахметов, О.В. Шиманська,
С.О. Рахметова, В.В. Фіщенко
Національний ботанічний сад
імені М.М. Гришка НАН України,
Україна, м. Київ
АНТИОКСИДАНТНА АКТИВНІСТЬ
ЕКСТРАКТІВ НАСІННЯ ДЕЯКИХ
КОРМОВИХ РОСЛИН
Мета — дослідити антиоксидантну активність екс
трактів насіння деяких кормових рослин в умовах На
ціонального ботанічного саду імені М.М. Гришка
НАН України.
Матеріал та методи. Досліджено насіння семи кор
мових рослин: Baptisia australis (L.) R.Br. (Fabaceae),
Bunias erucago L., B. orientalis L. (Brassicaceae), Galega
officinalis L., G. orientalis Lam. (Fabaceae), Isatis littoralis
Steven, I. tinctoria L. (Brassicaceae). Для визначення
ан тирадикальної активності рослинних екстрактів ви
користовували метод, описаний BrandWilliams та ін.
(1995). Біохімічний аналіз передбачав такі етапи: під
готовка метанольних, етанольних та водних екстрак
тів (1 г сухого матеріалу змішували з 25 мл розчинни
ка); 12 год екстракції; спектрофотометричне визна
чення антирадикальної активності з розчином 2,2ди
фенілпікрилгідразилу. Розчин радикалу готували в
метанолі та розводили згідно з методикою. Дані пере
раховано як еквівалент аскорбінової кислоти (АКЕ) і
тролоксу (ТЕ) на 1 г сухої маси. Вимірювання екстра
ктів проводили на спектрофотометрі UNICO UV 2800
за довжини хвилі 515 нм. Експериментальні дані
опрацьовано в програмі Excel.
Результати. Метанольні екстракти досліджуваних рос
лин мали антирадикальну активність 18,27—80,57 %,
етанольні — 11,07—79,73 %, водні — 23,31—80,26 %.
Антиоксидантна активність метанольних екстрактів ста
новила 1,75—2,72 мг AКE на 1 г, етанольних екстрак
тів — 1,62—2,72 мг AКE на 1 г, водних екстрактів —
1,82—2,66 мг AКE на 1 г, або відповідно 1,30—7,51,
0,56—7,76 та 1,19—7,37 мг TE на 1 г.
Висновки. Отримані дані демонструють, що в умо
вах Національного ботанічного саду НАН України
екстракти насіння досліджуваних кормових рослин
характеризуються високим антиоксидантним потен
ціалом, як і листки чи пагони. Антирадикальна ак
тивність екстрактів становила 11,07—84,60 %. Най
менші значення цього параметра зафіксовано в усіх
екстрактів. Антиоксидантна активність, виражена як
АКЕ, була максимальною для Bunias orientalis і Galega
officinalis та мінімальною — для Isatis tinctoria, а вира
жена як ТЕ була найвищою у метанольних екстрактів
Bunias orientalis та найнижчою — в етанольних екстрак
тів Bunias erucago. Дослідження екстрактів насіння
культур з різних родин є необхідним для оцінки їх ан
тиоксидантного потенціалу та рекомендацій щодо їх
використання.
Ключові слова: насіння, спиртові та водні екстракти, ан
тирадикальна активність, антиоксидантна активність.
О.Н. Вергун, Д.Б. Рахметов, О.В. Шиманская,
С.А. Рахметова, В.В. Фищенко
Национальный ботанический сад
имени Н.Н. Гришко НАН Украины,
Украина, г. Киев
АНТИОКСИДАНТНАЯ АКТИВНОСТЬ
ЭКСТРАКТОВ СЕМЯН НЕКОТОРЫХ
КОРМОВЫХ РАСТЕНИЙ
Цель — исследовать антиоксидантную активность
экстрактов семян некоторых кормовых растений в
условиях Национального ботанического сада имени
Н.Н. Гришко НАН Украины.
Материал и методы. Исследованы семена семи кор
мовых растений: Baptisia australis (L.) R.Br. (Fabaceae),
Bunias erucago L., B. orientalis L. (Brassicaceae), Galega
officinalis L., G. orientalis Lam. (Fabaceae), Isatis littoralis
Steven, I. tinctoria L. (Brassicaceae). Для определения
антирадикальной активности растительных экстрак
тов использовали метод, описанный BrandWilliams
и др. (1995). Биохимический анализ предусматривал
76 ISSN 16056574. Plant introduction, 2019, № 2
O.M. Vergun, D.B. Rakhmetov, O.V. Shymanska, S.O. Rakhmetova, V.V. Fishchenko
следующие этапы: подготовка метанольных, этаноль
ных и водных экстрактов (1 г сухого материала сме
шивали с 25 мл растворителя); 12 ч экстракции;
спектрофотометри ческое определение антирадикаль
ной активности с раствором 2,2дифенилпик рил гид
разила. Раствор ра дикала готовили в метаноле и раз
водили соответст венно методике. Данные пересчита
ны как эквивалент аскорбиновой кислоты (АКЭ) и
тролокса (ТЭ) на 1 г сухой массы. Измерение экстрак
тов проводили на спектрофотометре UNICO UV 2800
при длине волны 515 нм. Экспериментальные данные
обработаны в программе Excel.
Результаты. Метанольные экстракты исследован
ных растений имели антирадикальную активность
18,27—80,57 %, этанольные — 11,07—79,73 %, вод
ные — 23,31—80,26 %. Антиоксидантная активность
метанольных экстрактов 1,75——2,72 мг AКЭ на 1 г,
этанольных экстрактов — 1,62—2,72 мг AКЭ на 1 г,
водных экстрактов — 1,82—2,66 мг AКЭ на 1 г, или соот
ветственно 1,30—7,51, 0,56—7,76 и 1,19—7,37 мг TЭ
на 1 г.
Выводы. Полученные данные демонстрируют, что
в условиях Национального ботанического сада НАН
Украины экстракты семян исследованных кормовых
растений характеризуются высоким антиоксидант
ным потенциалом, как и листья или побеги. Антира
дикальная активность экстрактов составила 11,07—
84,60 %. Наименьшие значения этого параметра за
фиксированы у всех этанольных экстрактов. Антиок
сидантная активность, выраженная как АКЭ, была
максимальной для Bunias orientalis и Galega officinalis и
минимальной — для Isatis tinctoria, а выраженная как
ТЭ была наивысшей у метанольных экстрактов Bunias
orientalis и самой низкой — у этанольных экстрактов
Bunias erucago. Исследование экстрактов семян куль
тур из разных семейств является необходимым для
оценки их антиоксидантного потенциала и рекомен
даций относительно их использования.
Ключевые слова: семена, спиртовые и водные экстрак
ты, антирадикальная активность, антиоксидантная
активность.
|
| id | oai:ojs2.plantintroduction.org:article-985 |
| institution | Plant Introduction |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2025-07-17T12:48:12Z |
| publishDate | 2019 |
| publisher | M.M. Gryshko National Botanical Garden of the NAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | wwwplantintroductionorg/5e/196f10f2277ff96de6b42a6132a8d75e.pdf |
| spelling | oai:ojs2.plantintroduction.org:article-9852019-11-11T08:15:58Z Antioxidant activity of seed extracts of selected forage plants Антиоксидантна активність екстрактів насіння деяких кормових рослин Vergun, O.M. Rakhmetov, D.B. Shymanska, O.V. Rakhmetova, S.O. Fishchenko, V.V. Objective – to investigate the antioxidant activity of seed extracts of selected forage plants in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. Seeds of seven forage plants researched in this study: Baptisia australis (L.) R. Br. (Fabaceae), Bunias erucago L., B. orientalis L. (Brassicaceae), Galega officinalis L., G. orientalis Lam. (Fabaceae), Isatis littoralis Steven, I. tinctoria L. (Brassicaceae). The method described by Brand-Williams et al. (1995) used to determine the antiradical activity of plant extracts. Biochemical analyze included following stages: preparation of methanol, ethanol and water extracts (1 g of dried material mixed with 25 ml of solvent); 12 hours of extraction; spectrophotometric procedure of determination of antiradical activity with a 2,2-diphenyl-2-picrylhydrazyl solution. A solution of radical prepared in methanol and diluted according to method. Data were expressed in ascorbic acid equivalent (AAE) and Trolox equivalent (TE) on a gram of dry weight. Measurements of extracts carried out on spectrophotometer UNICO UV 2800 at 515 nm. Experimental data processed by Excel. Results. Methanol extracts of investigated plants had an antiradical activity of 18.27–80.57 %, ethanol extracts of 11.07–79.73 % and water extracts of 23.31–80.26 %. Antioxidant activity of methanol extracts expressed on ascorbic acid equivalent was 1.75–2.72 mg AAE per gram, ethanol extracts 1.62–2.72 mg AAE per gram and water extracts 1.82–2.66 mg AAE per gram. Results exhibited antioxidant activity expressed on Trolox equivalent of methanol extracts from 1.30 to 7.51 mg TE per gram, ethanol extracts from 0.56 to 7.76 mg TE per gram and water extracts from 1.19 to 7.37 mg TE per gram. Conclusions. Obtained data demonstrated that in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine seed extracts of investigated forage plants have high antioxidant potential as well as leaves or shoots. Antiradical activity of extracts was of 11.07–84.60 %. All seed extracts had the least values of this parameter in the ethanolic extracts. Antioxidant activity expressed in ascorbic acid equivalent was maximal for Bunias orientalis and Galega officinalis and minimal for Isatis tinctoria. Antioxidant activity by Trolox equivalent was high for Bunias orientalis methanol extracts and slow for Bunias erucago ethanol extracts. Investigation of seeds extracts of crops from different plant families is need to evaluate antioxidant potential of it and recommend for use. Мета — дослідити антиоксидантну активність екстрактів насіння деяких кормових рослин в умовах Національного ботанічного саду імені М.М. Гришка НАН України. Матеріал та методи. Досліджено насіння семи кормових рослин: Baptisia australis (L.) R. Br. (Fabaceae), Bunias erucago L., B. orientalis L. (Brassicaceae), Galega officinalis L., G. orientalis Lam. (Fabaceae), Isatis littoralis Steven, I. tinctoria L. (Brassicaceae). Для визначення антирадикальної активності рослинних екстрактів використовували метод, описаний Brand-Williams та ін. (1995). Біохімічний аналіз передбачав такі етапи: підготовка метанольних, етанольних та водних екстрактів (1 г сухого матеріалу змішували з 25 мл розчинника); 12 год екстракції; спектрофотометричне визначення антирадикальної активності з розчином 2,2-дифеніл-пікрилгідразилу. Розчин радикалу готували в метанолі та розводили згідно з методикою. Дані перераховано як еквівалент аскорбінової кислоти (АКЕ) і тролоксу (ТЕ) на 1 г сухої маси. Вимірювання екстрактів проводили на спектрофотометрі UNICO UV 2800 за довжини хвилі 515 нм. Експериментальні дані опрацьовано в програмі Excel. Результати. Метанольні екстракти досліджуваних рослин мали антирадикальну активність 18,27–80,57 %, етанольні – 11,07–79,73 %, водні – 23,31–80,26 %. Антиоксидантна активність метанольних екстрактів становила 1,75–2,72 мг AКE на 1 г, етанольних екстрактів – 1,62–2,72 мг AКE на 1 г, водних екстрактів – 1,82–2,66 мг AКE на 1 г, або відповідно 1,30–7,51, 0,56–7,76 та 1,19–7,37 мг TE на 1 г. Висновки. Отримані дані демонструють, що в умовах Національного ботанічного саду НАН України екстракти насіння досліджуваних кормових рослин характеризуються високим антиоксидантним потенціалом, як і листки чи пагони. Антирадикальна активність екстрактів становила 11,07–84,60 %. Найменші значення цього параметра зафіксовано в усіх екстрактів. Антиоксидантна активність, виражена як АКЕ, була максимальною для Bunias orientalis і Galega officinalis та мінімальною – для Isatis tinctoria, а виражена як ТЕ була найвищою у метанольних екстрактів Bunias orientalis та найнижчою – в етанольних екстрактів Bunias erucago. Дослідження екстрактів насіння культур з різних родин є необхідним для оцінки їх антиоксидантного потенціалу та рекомендацій щодо їх використання. M.M. Gryshko National Botanical Garden of the NAS of Ukraine 2019-06-01 Article Article application/pdf https://www.plantintroduction.org/index.php/pi/article/view/985 10.5281/zenodo.3241204 Plant Introduction; Vol 82 (2019); 71-76 Інтродукція Рослин; Том 82 (2019); 71-76 2663-290X 1605-6574 10.5281/zenodo.3377520 en https://www.plantintroduction.org/index.php/pi/article/view/985/946 http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Vergun, O.M. Rakhmetov, D.B. Shymanska, O.V. Rakhmetova, S.O. Fishchenko, V.V. Антиоксидантна активність екстрактів насіння деяких кормових рослин |
| title | Антиоксидантна активність екстрактів насіння деяких кормових рослин |
| title_alt | Antioxidant activity of seed extracts of selected forage plants |
| title_full | Антиоксидантна активність екстрактів насіння деяких кормових рослин |
| title_fullStr | Антиоксидантна активність екстрактів насіння деяких кормових рослин |
| title_full_unstemmed | Антиоксидантна активність екстрактів насіння деяких кормових рослин |
| title_short | Антиоксидантна активність екстрактів насіння деяких кормових рослин |
| title_sort | антиоксидантна активність екстрактів насіння деяких кормових рослин |
| url | https://www.plantintroduction.org/index.php/pi/article/view/985 |
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