COMPOSITION OF STRAINS BRADYRHIZOBIUM JAPONICUM AND ITS USE FOR SOYBEAN INOCULATION
In the vegetation experiment conditions it was established that the greatest effect of soybean bacterization can be obtained by two strains of nodule bacteria with slow (Bradyrhizobium japonicum 46) and intensive (B. japonicum КВ11) growth rates when these strains used in inoculum in the ratio 1 : 1...
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2016
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Agriciltural microbiology| _version_ | 1871465340596125696 |
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| author | Krutylo, D. V. |
| author_facet | Krutylo, D. V. |
| author_institution_txt_mv | [
{
"author": "D. V. Krutylo",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Krutylo, D. V. |
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| collection | OJS |
| datestamp_date | 2026-07-22T10:10:42Z |
| description | In the vegetation experiment conditions it was established that the greatest effect of soybean bacterization can be obtained by two strains of nodule bacteria with slow (Bradyrhizobium japonicum 46) and intensive (B. japonicum КВ11) growth rates when these strains used in inoculum in the ratio 1 : 1 (binary composition). In compared to the mono-inoculation the combined use of these strains contributed the formation of balanced symbiotic system, increase the level of molecular nitrogen fixation, content of chlorophyll in the biomass leaves and above ground mass of different soybean plants varieties on 7.8–19.6 %. In a field experiment inoculation of soybean seeds with a composition of B. japonicum strains to increase the soybean yields by 11.1–13.7 % compared to the mono-inoculation. |
| doi_str_mv | 10.35868/1997-3004.24.29-36 |
| first_indexed | 2025-07-17T12:23:06Z |
| format | Article |
| fulltext |
29
Сільськогосподарська мікробіологія. — 2016. — Вип. 24. — С. 29–36.
ISSN 1997-3004
UDC 635.652.2:631.847.211
COMPOSITION OF STRAINS BRADYRHIZOBIUM JAPONICUM
AND ITS USE FOR SOYBEAN INOCULATION
D. V. Krutylo
Institute of Agricultural Microbiology and Agroindustrial Manufacture, NAAS
97, Shevchenka str., Chernihiv, 14027, Ukraine; e-mail: krutilod@mail.ru
In the vegetation experiment conditions it was established that the greatest effect of soybean
bacterization can be obtained by two strains of nodule bacteria with slow (Bradyrhizobium
japonicum 46) and intensive (B. japonicum КВ11) growth rates when these strains used in inocu-
lum in the ratio 1 : 1 (binary composition). In compared to the mono-inoculation the combined use
of these strains contributed the formation of balanced symbiotic system, increase the level of mo-
lecular nitrogen fixation, content of chlorophyll in the biomass leaves and above ground mass of
different soybean plants varieties on 7.8–19.6 %. In a field experiment inoculation of soybean seeds
with a composition of B. japonicum strains to increase the soybean yields by 11.1–13.7 % com-
pared to the mono-inoculation.
Key words: Bradyrhizobium japonicum, binary composition of strains, competitiveness, inocu-
lation, yield, soybean.
An important reserve to increase producti-
vity of legumes is the use of rhizobia, which
functions in plant-microbial interaction are di-
verse: a molecular nitrogen fixation, improved
nutrition of plants, synthesis of biologically ac-
tive substances, increase of plant resistance to
the action of pathogens and abiotic stresses [1; 2].
It is known that in regions of intensive soy-
bean cultivation using biological preparations
based on rhizobia, soils form different popula-
tion of these microorganisms in terms of den-
sity. Local populations are considered as a
source of economically valuable strains, on the
other hand, they can act as competitors of bio-
agents of microbial preparations in soil [2–4].
During the study of populations of soybean
microsymbionts in soils of Ukraine, among their
representatives we found strains with increased
growth rate, which conventionally called
“strains of intense growth”. Method of analyti-
cal selection gave new highly effective strains
of rhizobia from soybean with slow (Brady-
rhizobium japonicum 46) and intensive (B. ja-
ponicum KB11) growth. It has been established
that the strains of intense growth are characte-
rized by increased saprophytic competence
(survival in soil) compared with slowly growing
rhizobia [5]. Property for better survival in soil
characteristic for rhizobia with intense growth
can be used in practice to develop new micro-
bial preparations with a wide range of perfor-
mance.
It should be noted that the list of biological
preparations for the plant production over the
past decade have expanded to include prepara-
tions based on microorganisms of various func-
tional groups and preparations of binary action,
obtained as a result of a combination of several
microorganisms [6]. Today, it has been experi-
mentally proved the significant stimulating ef-
fect of the mixed cultures of microorganisms on
the growth and development of plants. Com-
pared with pure cultures of diazotrophs, consor-
tia of microorganisms are characterized by in-
creased efficiency and stability of action on
plants, which makes the prospects of their use in
agricultural practice [7; 8].
The possibility of using biological prepara-
tions of several strains of rhizobia belonging to
the same species has been poorly studied. For
example, A. I. Chunderova et al. [9] in field ex-
periments with peas show that multistrain (two
to three strains) inoculation of peas gives higher
and more stable yields than monostrain. The au-
thors made a conclusion about the prospects of
multistrain preparations when growing peas.
We believe that the use of some highly effi-
cient strains of soybean rhizobia with different
© D. V. Krutylo, 2016 ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2016. — Вип. 24.
30
survival strategy in soil for biological prepara-
tions to inoculate soybean seeds can help to not
only increase productivity of the culture, but
also the formation of stable active population of
specific rhizobia in soil.
Considering the above, the objective of our
work was to study the effect of binary composi-
tion of the strains of soybean rhizobia with dif-
ferent rates of growth on soybean symbiotic per-
formance of different varieties.
Materials and methods. The objects of the
study were strains of soybean rhizobia with
slow (B. japonicum 46) and intensive (B. japo-
nicum KB11) growth, antisera to these strains,
and soybean varieties Ustia and Omega Vin-
nytska.
In vegetation experiments, we studied the
effect of binary composition of strains (B. japo-
nicum 46 + B. japonicum KB11) on the sym-
biotic parameters, growth, and development of
soybean plants.
Cultures of rhizobia were grown separately
in 250 mL flasks with legume medium on an
orbital shaker at 180–200 rpm at +28 ... + 30° C
for 72 hours. The titre of bacteria was
2–4 × 109 CFU/mL. We studied different ratios
of strains of B. japonicum 46 and B. japonicum
KB11 in the inoculum (1 : 1, 1 : 2, 2 : 1, 1 : 3,
3 : 1, 1 : 4, 4 : 1). Bacterial load in case of pre-
treatment of seeds in all variants was 200–
300 cells per 1 seed.
Experiments were conducted in the green-
house in 2 L vessels. As the substrate, sod-
podzolic soil (pHwat. 5.5–5.9; humus content —
1.0 % that of easily hydrolyzed nitrogen (by Ti-
urin and Kononova) — 55,0–60,0 mg; P2O5 —
150,0–160,0 mg and K2O (by Kirsanov) —
100,0–110,0 mg per 1 kg of soil), in which no
local soybean rhizobia were used. Soybean of
two varieties was cultivated: Ustia and Omega
Vinnytska. Frequency of experiment is fivefold.
Humidity was maintained at 60 % natural hu-
midity.
Efficiency of legume-rhizobia symbiosis
was assessed by the following parameters: con-
tent of dry matter content in the top, plant
height, nodule-forming ability and activity of
symbiotic nitrogen fixation. The content of pho-
tosynthetic pigments in leaves of plants was
measured in the flowering stage by spectropho-
tometry [10].
The competitiveness of the studied strains
(ratio of nodules formed by strain to the total
number of studied nodules of each variant, ex-
pressed in per cent) was determined by Gruber-
Widal agglutination method [11]. We used an-
tisera, obtained to slowly growing strain B. ja-
ponicum 46 and intensely growing strain B. ja-
ponicum KB11. To establish the serological
identity of strains of soybean rhizobia, selected
nodules were thoroughly washed with water.
Each nodule was crushed in a penicillin vial
with 1.2 mL of normal saline solution. The re-
sulting homogenate was used as an antigen in
agglutination with specific antisera. Forty-eight
nodules were analysed from each variant. The
following variants were used as the controls:
normal saline solution + antigen (homogenate of
nodules) and antiserum + antigen (pure culture
of rhizobia against which the antiserum was ob-
tained).
The effectiveness of combined use of two
strains of soybean rhizobia with slow and in-
tense growth was studied in the field at the zone
of Polissia of Ukraine (experimental field of the
Institute of Agricultural Microbiology and Ag-
roindustrial Manufacture of the NAAS). The
strains of B. japonicum and B. japonicum KB11
were used in ratio 1 : 1. Inoculation load was
200–300 cells per 1 seed. Soil — leached black
soil with small population of soybean rhizobia.
Frequency of experiment — fourfold. Area of
accounting field — 6 m2. Placement of fields —
randomised. Soybean of Ustia variety was culti-
vated.
The activity of symbiotic nitrogen fixation
was determined by acetylene ethylene method
[12] on the gas chromatograph Chrom-4 with a
flame ionization detector (column with β-β'-
oxydipropionitrile). The temperature of the
thermostat is 50 °C. Gas rate: hydrogen —
30 mL/min, nitrogen — 100 mL/min, air —
500 mL/min.
Processing of data was performed by com-
puter programs Microsoft Office Excel and Sta-
tistica 7.0
Results and discussion. Vegetation experi-
ment with soybean of Ustia variety was per-
formed on sod-podzolic soil without soybean
rhizobia, therefore, nodules were not formed on
the roots of plants in the control variant (without
inoculation) (Table 1).
Inoculation of soybean seeds separately for
strains of rhizobia with intense (B. japonicum
KB11) and slow (B. japonicum 46) growth
promoted formation of significant number of
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2016. — Вип. 24.
31
Table 1. Symbiotic parameters, growth and development of soybean of variety Ustia under
the effect of inoculation with binary composition of strains B. japonicum, vegetation experiment
Variants of experiment
Plant
height,
cm
Dry top,
g/plant
Number of
nodules,
U/plant
Weigh of
nodules,
g/plant
Activity of
symbiotic nitro-
gen fixation,
µg of N per plant
per hour
Without inoculation (control) 39.38 0.98 0 0 0
Inoculation with B. japonicum 46 45.26 1.29 13.22 0.25 11.18
Inoculation with
B. japonicum КВ11 44.17 1.23 7.61 0.18 9.42
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (1 : 1) 48.58 1.39 15.44 0.29 14.72
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (1 : 2) 47.13 1.32 16.72 0.25 10.59
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (2 : 1) 45.70 1.30 21.78 0.27 13.24
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (1 : 3) 44.12 1.33 16.00 0.29 11.92
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (3 : 1) 42.24 1.36 17.00 0.29 12.21
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (1 : 4) 44.11 1.30 15.33 0.28 9.42
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (4 : 1) 41.81 1.37 10.44 0.28 14.86
НІР05 2.03 0.07 2.33 0.03 2.27
Note. In this and subsequent tables, reliable rise regarding parameters with monoinoculation is in bold.
nodules (8 and 13 units / plant, respectively)
with high nitrogenase activity (9.42 and
11.18 µg N per plant per hour). A positive effect
of the studied cultures on such parameters as
plant height and their top has been noted.
Analysis of the obtained data showed that
the combined use of two strains of soybean
rhizobia with different rate of growth is more
effective than monoinoculation. Thus, in most
variants, regardless of the ratio of strains in the
inoculum, the use of binary composition of
B. japonicum 46 + B. japonicum KB11 provided
a significant increase in the number of nodules
(1.2–2.9 times) and their weight (1.1–1.6 times)
compared with monoinoculation. In addition,
the combination of two strains gave a significant
increase of the level of nitrogenase activity in
the nodules (1.3–1.6 times).
Better than most parameters (height and
top, weight of nodules and activity of symbiotic
nitrogen fixation), was combined use of strains
of B. japonicum 46 and B. japonicum KB11 in
equal proportions — 1 : 1. Reliable gain of the
top, weight of nodules and their nitrogenase ac-
tivity has been also noted upon domination of
slowly growing strain B. japonicum 46 in inocu-
lum, when its proportion compared with inten-
sely grown strain B. japonicum KB11 was 4 : 1.
It should be noted that in case of absolute domi-
nance of the strain with slow growth in nodules
(monoinoculation with B. japonicum 46), these
parameters were lower. That is, the interaction
of the host plant with two active strains of soy-
bean rhizobia forms more efficient symbiotic
systems than the use of each strain separately.
This fact can explain the synergistic effect of
the studied microorganisms which functionally
complement each other in binary composition.
Similar results were obtained in vegetation
experiment with soybean of Omega Vinnytska
variety. Concomitant use of slowly and in-
tensely growing strains had a positive effect on
symbiotic parameters, growth, and development
of soybean plants (Table 2).
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2016. — Вип. 24.
32
Table 2. Symbiotic parameters, growth and development of soybean of variety Omega Vin-
nytska under the effect of inoculation with binary composition of strains B. japonicum, vegeta-
tion experiment
Variants of experiment
Plant
height,
cm
Dry top,
g/plant
Number of
nodules,
U/plant
Weigh of
nodules,
g/plant
Activity of
symbiotic nitro-
gen fixation,
µg of N per plant
per hour
Without inoculation (control) 33.98 1.40 0 0 0
Inoculation with B. japonicum 46 43.21 2.06 21.50 0.38 13.83
Inoculation with
B. japonicum КВ11 43.07 2.02 21.33 0.38 13.24
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (1 : 1) 47.89 2.24 25.00 0.42 25.60
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (1 : 2) 44.99 2.11 20.61 0.38 12.07
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (2 : 1) 45.34 2.17 20.89 0.40 19.42
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (1 : 3) 45.57 2.06 20.94 0.39 14.72
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (3 : 1) 47.35 2.13 18.94 0.40 18.54
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (1 : 4) 46.37 2.05 18.94 0.35 13.54
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (4 : 1) 46.77 2.07 24.11 0.42 25.90
НІР05 1.96 0.13 3.32 0.03 2.62
When growing soybean variety Omega
Vinnytska, the same as in the experiment with
soybean variety Ustia, B. japonicum 46 and
B. japonicum KB11 in the ratio of 1 : 1 were
better for the most parameters. Compared to
monoinoculation, such binary composition sig-
nificantly contributed to increasing the number
of nodules (by 16.3–17.2 %), their weight (by
11.1 %), and nitrogenase activity (1.85–1.93 ti-
mes). Plant height was increased by 10.8–11.2 %,
and top — by 8.7–10.9 %.
The combination of two studied strains of
rhizobia in inoculum in the ratio of 4 : 1 also
had a positive effect on plant height of soybean
variety Omega Vinnytska, weight of nodules,
and activity of symbiotic nitrogen fixation. In
this variant, a significant increase in these pa-
rameters compared to monoinoculation has been
observed.
It should be noted that in both soybean va-
rieties, inoculation with studied rhizobia both
separately and as part of a binary composition,
gave a significant intensification of the synthe-
sis of chlorophyll (Table 3). For example, when
growing soybean variety Ustia, in variants with
different ratios of soybean rhizobia strains, con-
tent of chlorophyll a and sum of chlorophyll a
and b in the biomass of leaves were increased
by 3.5–16.6 % and 5.8–20.7 %, respectively,
compared to monoinoculation. The maximum
of these parameters (180.46 mg / 100 g and
224.51 mg / 100 g, respectively) was observed
in the variant with concomitant use of the
strains B. japonicum 46 and B. japonicum KB11
in the ratio of 1 : 1.
The greatest accumulation of photosyn-
thetic pigments in biomass of the leaves of soy-
bean of variety Omega Vinnytska was observed
under the use of strains of rhizobia B. japonicum
46 and B. japonicum KB11 in the ratio of 1 : 1
and 1 : 4. In other variants reliable, but some-
what lower rise of the content of chlorophyll a
and sum of chlorophyll a and b compared with
monoinoculation was obtained. Please note that
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2016. — Вип. 24.
33
Table 3. Content of chlorophyll in the biomass of the leaves of soybean of different varieties
at the influence of inoculation with binary composition of strains B. japonicum, vegetation expe-
riment
Variants of experiment
Amount of chlorophyll, mg/100 g
Ustia variety Omega Vinnytska variety
а b а + b а b а + b
Without inoculation (control) 99.83 27.13 126.96 56.59 15.65 72.24
Inoculation with B. japonicum 46 164.97 47.30 212.27 93.39 27.13 120.53
Inoculation with
B. japonicum КВ11 154.74 31.24 185.98 98.00 29.39 127.39
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (1 : 1) 180.46 44.06 224.51 134.46 38.56 173.03
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (1 : 2) 167.06 46.86 213.91 128.22 30.09 158.31
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (2 : 1) 160.14 50.69 210.84 111.74 35.67 147.41
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (1 : 3) 163.77 47.61 211.38 120.19 35.97 156.16
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (3 : 1) 188.70 33.43 222.12 114.81 33.79 148.59
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (1 : 4) 167.29 52.61 219.90 128.19 39.55 167.74
Inoculation with B. japonicum 46
+ B. japonicum КВ11 (4 : 1) 160.55 42.09 202.64 116.41 32.54 148.95
НІР05 9.43 8.76 12.19 4.41 3.48 6.68
in case of combination of two strains in inocu-
lum in the ratio of 4 : 1, despite of the growing
of symbiotic parameters, content of chlorophyll
in the leaves of plants is increased only in soy-
bean of variety Omega Vinnytska.
For better understanding of the effect of
rhizobia on formation of symbiotic system, we
determined the proportion of strains B. japoni-
cum 46 and B. japonicum KB11 in nodules of
soybean of varieties Ustia and Omega Vinnyt-
ska (Table 4).
Studies have shown that after the use of
strains B. japonicum 46 and B. japonicum 46
KB11 in the ratio of 1 : 1 in both varieties of
soybean, number of root nodules formed by
these microorganisms was almost similar —
54.2 % : 45.8 % and 45.8 % : 54.2 %. It has
been established that increasing the proporti-
on of a particular strain in binary composition
(1 → 2 → 3 → 4) provided corresponding in-
crease of its proportion in nodules. After inocula-
tion with B. japonicum 46 + B. japonicum KB11
(1 : 2), distribution of strains in nodules was —
25.0 % : 75.0 %, whereas in case of proportion
of strains B. japonicum 46 and B. japonicum
KB11 (2 : 1) — 83.3 % : 16.7 %, respectively.
The only exception were two variants: on soy-
bean of variety Ustia with the use of strains
B. japonicum 46 and B. japonicum KB11 in the
ratio of 3 : 1 (their proportion in nodules was
similar — 50.0 % : 50.0 %) on soybean of va-
rieties Omega Vinnytska with a ratio of strains
of 2 : 1 (their proportion amounted to 41.7 % :
: 58.3 %). That is, the dominance of a particular
strain of rhizobia in the inoculum is not always
ensured its dominance in the nodules.
It should be noted that regardless of the ra-
tion of rhizobia in binary compositions, there
was no full replacement of one strain by another
one from the nodule populations. Each strain
occupied its ecological niche, which may indi-
cate the presence of close mutual regulation of
the amount of symbiotic nitrogen fixers in the
rhizosphere of soybean, as well as their ability
to compete for specific binding sites on the
roots of plants.
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2016. — Вип. 24.
34
Table 4. Portion of strains B. japonicum 46 and B. japonicum КВ11 in nodules of soybean
varieties Ustia and Omega Vinnytska, vegetation experiments
Variants of experiment
Proportion of strains of soybean rhizobia in nodules, %
Ustia Omega Vinnytska
B. japoni-
cum 46
B. japoni-
cum КВ11
B. japoni-
cum 46
B. japoni
cum КВ11
Without inoculation (control) 0 0 0 0
Inoculation with B. japonicum 46 100.0 0 100.0 0
Inoculation with B. japonicum
КВ11 0 100.0 0 100.0
Inoculation with B. japonicum 46 +
B. japonicum КВ11 (1 : 1) 54.2 45.8 45.8 54.2
Inoculation with B. japonicum 46 +
B. japonicum КВ11 (1 : 2) 25.0 75.0 33.3 66.7
Inoculation with B. japonicum 46 +
B. japonicum КВ11 (2 : 1) 83.3 16.7 41.7 58.3
Inoculation with B. japonicum 46 +
B. japonicum КВ11 (1 : 3) 12.5 87.5 4.2 95.8
Inoculation with B. japonicum 46 +
B. japonicum КВ11 (3 : 1) 50.0 50.0 79.2 20.8
Inoculation with B. japonicum 46 +
B. japonicum КВ11 (1 : 4) 12.5 87.5 8.3 91.7
Inoculation with B. japonicum 46 +
B. japonicum КВ11 (4 : 1) 79.2 20.8 75.0 25.0
The effectiveness of soybean inoculation
with strains of B. japonicum 46 and B. japoni-
cum KB11 and their binary composition (ratio
of strains of 1 : 1) was studied in a field experi-
ment on the background of a small local popula-
tion of specific rhizobia.
Inoculation of soybean seeds with studied
strains helped to increase productivity of culture
by 14.3–17.0 % compared to control (Table 5).
The combined use of these strains in binary
composition — B. japonicum 46 + B. japoni-
cum KB11 was more efficient. In this variant,
we received the maximum yield of soybean
grain — 2.90 t/ha.
Thus, the results of the conducted studies
showed that the greatest effect from soybean
bacterization can be obtained by a combination
of two strains of rhizobia with slow (B. japoni-
cum 46) and intensive (B. japonicum KB11)
growth in the ratio of 1 : 1 (binary composition)
in inoculum. Concomitant use of strains con-
tributed to the formation of balanced symbiotic
system, raising the level of fixation of molecular
nitrogen, chlorophyll content in biomass of lea-
ves and top of soybean of different varieties by
7.8–19.6 % compared with parameters in case
of monoinoculation.
Table 5. Seed productivity of soybean of
variety Ustia under the effect of combined ino-
culation with slowly and intensely growing
strains of rhizobia, field experiment, 2013
Варіанти досліду Productivity,
t/ha
Yield
raise, %
Without inoculation
(control) 2.23 –
Inoculation with
B japonicum 46 2.61 17.0
Inoculation with
B. japonicum КВ11 2.55 14.3
Inoculation with
B. japonicum 46
+ B. japonicum КВ11
2.90 30.0
НІР05 0.21
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2016. — Вип. 24.
35
Against the background of soil population
of specific rhizobia, inoculation of soybean by
the composition of strains B. japonicum 46 +
B. japonicum KB11 provided increase in pro-
ductivity of culture by 11.1–13.7 % compared to
monoinoculation.
Further studies of binary composition of the
strains B. japonicum are promising from a sci-
entific and practical point of view. They allow
reveal the deeper features of formation and fun-
ctioning of efficient symbiotic systems under
the infection of soybean by two strains both at
the absence of soybean rhizobia in the soil, and
at the background of the local populations of
rhizobia.
1. Тихонович И. А. Значение биологической
фиксации азота для современной биологии и
практики сельскохозяйственного производства /
И. А. Тихонович // Аграрная наука. — 1993. —
№ 3. — С. 29–31.
2. Биологическая фиксация азота: бобово-
ризобиальный симбиоз : [монография : в 4-х т.] /
[С. Я. Коць, В. В. Моргун, В. Ф. Патыка и
др.]. — Т. 2. — К. : Логос, 2011. — 523 с.
3. Толкачев Н. З. Потенциальные возможно-
сти симбиотической азотфиксации при выращи-
вании сои на юге Украины / Н. З. Толкачев //
Мікробіол. журн. — 1997. — Т. 59, № 4. —
С. 34–41.
4. Патика В. П. Вплив аборигенних популя-
цій бульбочкових бактерій сої на симбіотичну
активність інтродукованого штаму Bradyrhizo-
bium japonicum 634б / В. П. Патика, Д. В. Крути-
ло, Т. М. Ковалевська // Мікробіол. журн. —
2004. — Т. 66, № 3. — С. 14–21.
5. Штам бульбочкових бактерій сої з підви-
щеною сапрофітною компетентністю як основа
біопрепаратів / [Д. В. Крутило, М. А. Ушакова,
С. І. Колісник та ін.] // Корми і кормовироб-
ництво. — 2015. — Вип. 80. — С. 59–65.
6. Сытников Д. М. Биотехнология микроор-
ганизмов-азотфиксаторов и перспективы при-
менения препаратов на их основе / Д. М. Сытни-
ков // Біотехнологія. — 2012. — Т. 5, № 4. —
С. 34–45.
7. Злотников А. К. Взаимосвязь нитрогеназ-
ной активности, устойчивости и относительного
содержаний компонентов смешанных культур
диазотрофных бактерий / А. К. Злотников,
О. Б. Глаголева, М. М. Умаров // Микробиоло-
гия. — 1997. — Т. 66, № 6. — С. 807–812.
8. Masciarelli O. A new PGPR co-inoculated
with Bradyrhizobium japonicum enhances soybean
nodulation / O. Masciarelli, A. Llanes, V. Luna //
Microbiol. Res. — 2014. — Vol. 169, № 8. —
P. 609–615.
9. Эффективность полиштаммовой иноку-
ляции гороха / А. И. Чундерова, Т. И. Сели-
верстова, С. М. Алисова, И. Ф. Орлова // Тр.
ВНИИСХМ. — Л., 1978. — Т. 47. — С. 74–83.
10. Гродзинский А. М. Краткий справочник
по физиологии растений / А. М. Гродзинский,
Д. М. Гродзинский. — К. : Наукова думка,
1973. — 398 с.
11. Кэбот Э. Экспериментальная иммуноло-
гия / Э. Кэбот, Б. Мейер. — М. : Медицина,
1968. — 677 с.
12. The acetylene-ethylene assay for N2-fi-
xation: Laboratory and field evaluation /
R. W. F. Hardy, R. D. Holsten, E. K. Jackson,
R. C. Burns // Plant Physiol. — 1968. — Vol. 43,
№ 8. — P. 1185–1207.
КОМПОЗИЦІЯ ШТАМІВ BRADYRHI-
ZOBIUM JAPONICUM ТА ЇЇ ЗАСТОСУ-
ВАННЯ ДЛЯ ІНОКУЛЯЦІЇ СОЇ
Д. В. Крутило
Інститут сільськогосподарської мікробіології та
агропромислового виробництва НААН,
м. Чернігів
За умов вегетаційних дослідів встанов-
лено, що найбільший ефект від бактеризації
сої можна отримати при поєднанні в іноку-
люмі двох штамів бульбочкових бактерій з
повільним (Bradyrhizobium japonicum 46) та
інтенсивним (B. japonicum КВ11) ростом у
співвідношенні 1 : 1 (бінарна композиція).
КОМПОЗИЦИЯ ШТАММОВ BRADYRH-
IZOBIUM JAPONICUM И ЕЁ ПРИМЕНЕ-
НИЕ ДЛЯ ИНОКУЛЯЦИИ СОИ
Д. В. Крутило
Институт сельскохозяйственной микробиологии
и агропромышленного производства НААН,
г. Чернигов
В условиях вегетационных опытов пока-
зано, что наибольший эффект от бактери-
зации сои можно получить при сочетании в
инокулюме двух штаммов клубеньковых
бактерий с медленным (Bradyrhizobium ja-
ponicum 46) и интенсивным (B. japonicum
КВ11) ростом в соотношении 1 : 1 (бинар-
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2016. — Вип. 24.
36
Сумісне використання цих штамів сприяло
формуванню збалансованої симбіотичної си-
стеми, підвищенню рівня фіксації молекуля-
рного азоту, вмісту хлорофілів у біомасі
листя та надземної маси рослин сої різних
сортів на 7,8–19,6 % відносно моноінокуля-
ції. У польовому досліді інокуляція насіння
сої композицією штамів B. japonicum сприя-
ла збільшенню урожайності сої на 11,1–
13,7 % порівняно з моноінокуляцією.
Ключові слова: Bradyrhizobium japoni-
cum, бінарна композиція штамів, інокуляція,
врожайність, соя.
ная композиция). Совместное использование
штаммов способствовало формированию
сбалансированной симбиотической систе-
мы, повышению уровня фиксации молекуляр-
ного азота, содержания хлорофиллов в био-
массе листьев и надземной массы растений
сои различных сортов на 7,8–19,6 % отно-
сительно моноинокуляции. В полевом опыте
инокуляция семян сои композицией штаммов
B. japonicum способствовала увеличению
урожайности сои на 11,1–13,7 % по сравне-
нию с моноинокуляцией.
Ключевые слова: Bradyrhizobium japoni-
cum, бинарная композиция штаммов, иноку-
ляция, урожайность, соя.
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2016. — Вип. 24.
|
| id | oai:ojs2.smic.in.ua:article-112 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2026-07-23T01:00:29Z |
| publishDate | 2016 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/d7/f34fab9d0d4f2d2aec8fa5ab7b61acd7.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1122026-07-22T10:10:42Z COMPOSITION OF STRAINS BRADYRHIZOBIUM JAPONICUM AND ITS USE FOR SOYBEAN INOCULATION COMPOSITION OF STRAINS BRADYRHIZOBIUM JAPONICUM AND ITS USE FOR SOYBEAN INOCULATION Krutylo, D. V. Bradyrhizobium japonicum, binary composition of strains, competitiveness, inoculation, yield, soybean Bradyrhizobium japonicum, binary composition of strains, competitiveness, inoculation, yield, soybean In the vegetation experiment conditions it was established that the greatest effect of soybean bacterization can be obtained by two strains of nodule bacteria with slow (Bradyrhizobium japonicum 46) and intensive (B. japonicum КВ11) growth rates when these strains used in inoculum in the ratio 1 : 1 (binary composition). In compared to the mono-inoculation the combined use of these strains contributed the formation of balanced symbiotic system, increase the level of molecular nitrogen fixation, content of chlorophyll in the biomass leaves and above ground mass of different soybean plants varieties on 7.8–19.6 %. In a field experiment inoculation of soybean seeds with a composition of B. japonicum strains to increase the soybean yields by 11.1–13.7 % compared to the mono-inoculation. In the vegetation experiment conditions it was established that the greatest effect of soybean bacterization can be obtained by two strains of nodule bacteria with slow (Bradyrhizobium japonicum 46) and intensive (B. japonicum КВ11) growth rates when these strains used in inoculum in the ratio 1 : 1 (binary composition). In compared to the mono-inoculation the combined use of these strains contributed the formation of balanced symbiotic system, increase the level of molecular nitrogen fixation, content of chlorophyll in the biomass leaves and above ground mass of different soybean plants varieties on 7.8–19.6 %. In a field experiment inoculation of soybean seeds with a composition of B. japonicum strains to increase the soybean yields by 11.1–13.7 % compared to the mono-inoculation. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2016-10-09 Article Article Рецензована Стаття application/pdf https://smic.in.ua/index.php/journal/article/view/112 10.35868/1997-3004.24.29-36 Agricultural microbiology; Vol. 24 (2016): Agriciltural microbiology; 29-36 Сільськогосподарська мікробіологія; Том 24 (2016): Сільськогосподарська мікробіологія; 29-36 1997-3004 10.35868/1997-3004.24 en https://smic.in.ua/index.php/journal/article/view/112/115 Авторське право (c) 2016 D. V. Krutylo https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Bradyrhizobium japonicum binary composition of strains competitiveness inoculation yield soybean Krutylo, D. V. COMPOSITION OF STRAINS BRADYRHIZOBIUM JAPONICUM AND ITS USE FOR SOYBEAN INOCULATION |
| title | COMPOSITION OF STRAINS BRADYRHIZOBIUM JAPONICUM AND ITS USE FOR SOYBEAN INOCULATION |
| title_alt | COMPOSITION OF STRAINS BRADYRHIZOBIUM JAPONICUM AND ITS USE FOR SOYBEAN INOCULATION |
| title_full | COMPOSITION OF STRAINS BRADYRHIZOBIUM JAPONICUM AND ITS USE FOR SOYBEAN INOCULATION |
| title_fullStr | COMPOSITION OF STRAINS BRADYRHIZOBIUM JAPONICUM AND ITS USE FOR SOYBEAN INOCULATION |
| title_full_unstemmed | COMPOSITION OF STRAINS BRADYRHIZOBIUM JAPONICUM AND ITS USE FOR SOYBEAN INOCULATION |
| title_short | COMPOSITION OF STRAINS BRADYRHIZOBIUM JAPONICUM AND ITS USE FOR SOYBEAN INOCULATION |
| title_sort | composition of strains bradyrhizobium japonicum and its use for soybean inoculation |
| topic | Bradyrhizobium japonicum binary composition of strains competitiveness inoculation yield soybean |
| topic_facet | Bradyrhizobium japonicum binary composition of strains competitiveness inoculation yield soybean Bradyrhizobium japonicum binary composition of strains competitiveness inoculation yield soybean |
| url | https://smic.in.ua/index.php/journal/article/view/112 |
| work_keys_str_mv | AT krutylodv compositionofstrainsbradyrhizobiumjaponicumanditsuseforsoybeaninoculation |