ФУНКЦІОНАЛЬНА ТА ТАКСОНОМІЧНА СТРУКТУРА МІКРОБНОГО УГРУПОВАННЯ ТЕМНО-СІРОГО ҐРУНТУ
We analyzed the functional and taxonomic structure of microbial community of dark grey soil, depending on the effects of the weather and fertilizer systems. It was shown that fallow soil is characterized by integrated and more stable microbial community compared with agroecosystems soil. However, un...
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| Date: | 2016 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English Ukrainian |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2016
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| Online Access: | https://smic.in.ua/index.php/journal/article/view/114 |
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| Journal Title: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465359839592448 |
|---|---|
| author | Шерстобоєва, О. В. Дем’янюк, О. С. |
| author_facet | Шерстобоєва, О. В. Дем’янюк, О. С. |
| author_institution_txt_mv | [
{
"author": "О. В. Шерстобоєва",
"institution": "Інститут агроекології і природокористування НААН"
},
{
"author": "О. С. Дем’янюк",
"institution": "Інститут агроекології і природокористування НААН"
}
] |
| author_sort | Шерстобоєва, О. В. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:42Z |
| description | We analyzed the functional and taxonomic structure of microbial community of dark grey soil, depending on the effects of the weather and fertilizer systems. It was shown that fallow soil is characterized by integrated and more stable microbial community compared with agroecosystems soil. However, under the effect of such abiotic factors as increased average monthly temperatures and moisture deficiency, some imbalance of trophic interactions in microbiocenosis is observed.
Agroecosystems are characterized by less stable functional and taxonomic structure of the soil, which is defined by the hydrothermal conditions of the growing season and types of fertilizers applied. This is supported by a smaller number of correlations and a simplified structure of correlation pleiades of the soil of agroecosystems during unfavourable periods of vegetation periods, especially in the soil without fertilizers and using only mineral fertilizers. The use of organic and mineral fertilizer system brings the state of the soil microbiocenosis of agroecosystems soil to that of fallow soil. |
| doi_str_mv | 10.35868/1997-3004.24.43-51 |
| first_indexed | 2025-07-17T12:23:07Z |
| format | Article |
| fulltext |
TAXONOMIC AND FUNCTIONAL STRUCTURE OF THE MICROBIAL
COMMUNITIES OF DARK GREY SOIL
O. V. Sherstoboeva, O. S. Demianiuk
Institute of Agroeсology and Environmental Management, NAAS,
City of Kyiv
We analyzed the functional and taxonomic structure of microbial community of dark grey
soil, depending on the effects of the weather and fertilizer systems. It was shown that fallow soil
is characterized by integrated and more stable microbial community compared with
agroecosystems soil. However, under the effect of such abiotic factors as increased average
monthly temperatures and moisture deficiency, some imbalance of trophic interactions in
microbiocenosis is observed.
Agroecosystems are characterized by less stable functional and taxonomic structure of the
soil, which is defined by the hydrothermal conditions of the growing season and types of
fertilizers applied. This is supported by a smaller number of correlations and a simplified
structure of correlation pleiades of the soil of agroecosystems during unfavourable periods of
vegetation periods, especially in the soil without fertilizers and using only mineral fertilizers.
The use of organic and mineral fertilizer system brings the state of the soil microbiocenosis of
agroecosystems soil to that of fallow soil.
Keywords: microbial communities, dark grey bleached soil, functional and taxonomic
structure, weather conditions, fertilizer system.
Studies of the influence of climate on
soil formation processes and soil properties
were conducted from the time of work of
V. Dokuchaiev, however, many not fully
clarified aspects still exist. This is issue is of
particular relevance in the context of
changes on climate, since soil and its
organic part contains significant amount of
carbon. While soil itself and soil cover,
according to V. Rozhkov [1], are witness
and indicators of the global climate change.
Climate change directly affect energy
level and hydrothermal regimen of soil, and
indirectly through effects on other soil
formation factors – plants, vital activity of
organisms, etc. [2]. Given the crucial value
of microbiological component of soil in
biogeochemical circulation of carbon,
nitrogen and other elements and global
flows of greenhouse gases (CO2, CH4, and
N2O), considerable scientific interest is paid
to the reaction of microbiota, its biochemical
activity and soil fertility on changes in the
climatic parameters [3-5]. Microbiological
processes depend on the environmental
factors such as temperature, humidity, CO2
concentration in atmosphere, availability of
nutrients, all of which are likely to be
affected by climate change [6].
In this regard, the aim was to study
functional and taxonomic structure of
microbial communities of dark gray soil
under different hydrothermal conditions and
application of fertilizers.
Materials and methods. To
determine functional and taxonomic
structure of microbiocenosis of dark gray
soil, experimental data of studies obtained at
the Microorganisms Ecology Laboratory of
the Institute of Agroecology and
Environmental Management of the NAAS
were used [7; 8]. Samples of soil were
selected in the stationary field experiment at
the Institute of Agriculture of West
Woodlands of the NAAS (Rivne State
Agricultural Experimental Station) with the
following options: 1 – without fertilizer
(control), 2 – mineral fertilization system
(NPK), 3 – organic mineral fertilization
system (manure + NPK) and at the
surrounding territory – natural ecosystem
(fallow).
Sampling of soil for microbiological
tests was conducted from topsoil 0-20 cm
between rows of sugar beets in July, when
the system reaches steady state [9]. Soil type
– dark gray bleached; pHsal. – 5.8; humus
content – 1.8 %; easily hydrolysed nitrogen
compounds – 117 mg/kg of soil; mobile
phosphorus – 235 mg/kg of soil;
exchangeable potassium – 87.5 mg/kg of
soil.
The number of microorganisms of
the main functional and taxonomic groups
was measured by common method for soil
microbiology seeding of successive dilutions
of water-soil suspension on standard digest
media [10; 11] eutrophic plants (EU) that
use nitrogen of organic compounds – on
meat-and-peptone agar; microorganisms
using nitrogen of mineral compounds (EM)
– on starch-ammonia agar (SAA);
pedotrophs (PT) – on soil agar (SAg);
nitrogen fixing bacteria (NFB) – on
Vynohradskyi nitrogen-free medium;
oligotrophic microorganisms (OT) – on
starvation agar (SA); cellulolytic
microorganisms (CL) – on Vynohradskyi
medium with cellulose modified by
Pushkinska; nitrifying (NF) – on starvation
agar with ammonium magnesium salt; total
bacterial count – on peptone-glucose agar
with soil extract; micromycetes – on Czapek
medium at pH 5.0; streptomycetes – on
starch ammonium agar.
To evaluate the functional structure
of soil microbiocenosis depending on the
type of soil fertilizers and weather
conditions, the modified method of
correlation pleiades [12; 13] and the
corresponding software for their plotting
developed at Harvard University was used
[14] Mathematical processing and statistical
analysis of the results obtained during
studies was performed under the
recommendations of manuals on statistical
analysis of experimental results and
software “Statistica”, Microsoft Office
Excel.
To characterize the hydrothermal
regime of vegetation period, data of Rivne
regional meteorological station were used, at
the same times years for which contrast in
summer air temperature differential and the
amount of rainfall during the period of
measurement of quantitative characteristics
of soil microbial communities were selected.
In particular, analysis of meteorological data
for the Region of Rivne showed excess in air
temperature in July 2004 by 1.4 °C from
average long-standing level (ALL) (Fig. 1).
In addition, during the next years of study,
annual temperature increase by 0.5 °C took
place. As for humidity, 2004 was the most
water-deprived, when moisture deficiency
was 28 mm. In 2005, when slight water-
deprivation was observed, rain fell on
14 mm lesser during ALL. In 2006, there
was excessively wet weather, the excess of
rain from ALL this year amounted to
45 mm.
Thus, July 2004 was extremely
moisture-depleted, 2006 – hot with
excessive moisture, 2005 – arid, but
approaching the ALL.
Thus, for fallow soil integrated and
more sustainable microbial community is
typical compared with agro-ecosystem soil.
However, under the action of such abiotic
factors as increased monthly average
temperatures and lack of moisture, some
imbalance is observed in the trophic
relations of microbiocenosis.
Less stable functional and taxonomic
structure of soil is typical for agro-
ecosystem, which is defined both by
hydrothermal conditions of the vegetation
period and the types of fertilizers applied.
This is confirmed by lesser number of
correlation interactions and simplified
structure of correlation pleiades of agro-
ecosystem soil under unfavourable periods
of vegetation.
Structure of soil microbiocenosis
without application of fertilizers and under
use of mineral fertilizers is the most
vulnerable to weather factors. Due to the
ability of many microorganisms to move
from energy-intensive processes of ensuring
their nutritional needs to assimilation of
readily available forms of biogenic elements
from fertilizers, application of mineral
fertilizers introduction causes rupture of
many trophic relationships in microbial
community. The structure of correlation
pleiades suggests that the application of
organic mineral fertilizers contributes to the
increased stability of the microbial
communities of soil and approaches it to the
parameters of soil of natural ecosystem.
Fig. 1. Monthly average values of air temperature (a) and amount of rain (б) in July 2004-2006,
Region of Rivne, Village of Shubkiv.
Note: ALL – average long-standing level.
Years Years
ALL ALL
Te
m
pe
ra
tu
re
s,
°C
A
m
ou
nt
o
f r
ai
n,
m
m
|
| id | oai:ojs2.smic.in.ua:article-114 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:00:47Z |
| publishDate | 2016 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/c2/f7d938c3a10233b1f63d90dbe65fa5c2.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1142026-07-22T10:10:42Z TAXONOMIC AND FUNCTIONAL STRUCTURE OF THE MICROBIAL COMMUNITIES OF DARK GREY SOIL ФУНКЦІОНАЛЬНА ТА ТАКСОНОМІЧНА СТРУКТУРА МІКРОБНОГО УГРУПОВАННЯ ТЕМНО-СІРОГО ҐРУНТУ Шерстобоєва, О. В. Дем’янюк, О. С. microbial communities, dark grey bleached soil, functional and taxonomic structure, weather conditions, fertilizer system мікробні угруповання, темно-сірий ґрунт, функціональна і таксономічна структура, погодні умови, система удобрення We analyzed the functional and taxonomic structure of microbial community of dark grey soil, depending on the effects of the weather and fertilizer systems. It was shown that fallow soil is characterized by integrated and more stable microbial community compared with agroecosystems soil. However, under the effect of such abiotic factors as increased average monthly temperatures and moisture deficiency, some imbalance of trophic interactions in microbiocenosis is observed. Agroecosystems are characterized by less stable functional and taxonomic structure of the soil, which is defined by the hydrothermal conditions of the growing season and types of fertilizers applied. This is supported by a smaller number of correlations and a simplified structure of correlation pleiades of the soil of agroecosystems during unfavourable periods of vegetation periods, especially in the soil without fertilizers and using only mineral fertilizers. The use of organic and mineral fertilizer system brings the state of the soil microbiocenosis of agroecosystems soil to that of fallow soil. Проведено аналіз функціональної і таксономічної структури мікробного угрупованнятемно-сірого опідзоленого ґрунту залежно від впливу погодних умов і застосованих добрив.Встановлено, що для ґрунту перелогу характерне інтегроване і більш стійке мікробне угруповання, ніж для ґрунту агроекосистем. Проте за дії таких абіотичних чинників як підвищені середньомісячні температури та нестача вологи спостерігається певне розбалансування трофічних зв’язків у мікробоценозі. Для агроекосистем характерним є менш стійка функціональна та таксономічна структура ґрунту, яка визначається як гідротермічними умовами вегетаційного періоду, так івидами внесених добрив. Це підтверджується меншою кількістю кореляційних зв’язків таспрощеною структурою кореляційних плеяд ґрунту агроекосистем у несприятливі періодивегетації рослин, особливо у ґрунті без удобрення та за внесення лише мінеральних добрив.Використання органо-мінеральної системи удобрення наближує стан мікробоценозу ґрунтуагроекосистеми до показників ґрунту перелогу. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2016-10-09 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/114 10.35868/1997-3004.24.43-51 Agricultural microbiology; Vol. 24 (2016): Agriciltural microbiology; 43-51 Сільськогосподарська мікробіологія; Том 24 (2016): Сільськогосподарська мікробіологія; 43-51 1997-3004 10.35868/1997-3004.24 en uk https://smic.in.ua/index.php/journal/article/view/114/118 https://smic.in.ua/index.php/journal/article/view/114/119 Авторське право (c) 2016 O. V. Sherstoboeva, O. S. Demianiuk https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | мікробні угруповання темно-сірий ґрунт функціональна і таксономічна структура погодні умови система удобрення Шерстобоєва, О. В. Дем’янюк, О. С. ФУНКЦІОНАЛЬНА ТА ТАКСОНОМІЧНА СТРУКТУРА МІКРОБНОГО УГРУПОВАННЯ ТЕМНО-СІРОГО ҐРУНТУ |
| title | ФУНКЦІОНАЛЬНА ТА ТАКСОНОМІЧНА СТРУКТУРА МІКРОБНОГО УГРУПОВАННЯ ТЕМНО-СІРОГО ҐРУНТУ |
| title_alt | TAXONOMIC AND FUNCTIONAL STRUCTURE OF THE MICROBIAL COMMUNITIES OF DARK GREY SOIL |
| title_full | ФУНКЦІОНАЛЬНА ТА ТАКСОНОМІЧНА СТРУКТУРА МІКРОБНОГО УГРУПОВАННЯ ТЕМНО-СІРОГО ҐРУНТУ |
| title_fullStr | ФУНКЦІОНАЛЬНА ТА ТАКСОНОМІЧНА СТРУКТУРА МІКРОБНОГО УГРУПОВАННЯ ТЕМНО-СІРОГО ҐРУНТУ |
| title_full_unstemmed | ФУНКЦІОНАЛЬНА ТА ТАКСОНОМІЧНА СТРУКТУРА МІКРОБНОГО УГРУПОВАННЯ ТЕМНО-СІРОГО ҐРУНТУ |
| title_short | ФУНКЦІОНАЛЬНА ТА ТАКСОНОМІЧНА СТРУКТУРА МІКРОБНОГО УГРУПОВАННЯ ТЕМНО-СІРОГО ҐРУНТУ |
| title_sort | функціональна та таксономічна структура мікробного угруповання темно-сірого ґрунту |
| topic | мікробні угруповання темно-сірий ґрунт функціональна і таксономічна структура погодні умови система удобрення |
| topic_facet | microbial communities dark grey bleached soil functional and taxonomic structure weather conditions fertilizer system мікробні угруповання темно-сірий ґрунт функціональна і таксономічна структура погодні умови система удобрення |
| url | https://smic.in.ua/index.php/journal/article/view/114 |
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