ОПТИМІЗАЦІЯ СКЛАДУ ЖЕЛЮЮЧИХ АГЕНТІВ НА РЕГЕНЕРАЦІЮ МІКРОРОСЛИН КАРТОПЛІ, ОТРИМАНИХ IN VITRO
Results of studies on the optimization of Murashige and Skoog culture medium withmodified maize starch for the acceleration of potato plants micropropogation are provided. Ithas been established that the method modification increases surveillance parameters, promotesactive in vitro growth and develo...
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| Datum: | 2016 |
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| Format: | Artikel |
| Sprache: | Englisch Ukrainisch |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2016
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| Online Zugang: | https://smic.in.ua/index.php/journal/article/view/119 |
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| Назва журналу: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465362715836416 |
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| author | Шевага, Г. М. Кирик, М. М. Гунчак, В. М. Олійник, Т. М. |
| author_facet | Шевага, Г. М. Кирик, М. М. Гунчак, В. М. Олійник, Т. М. |
| author_institution_txt_mv | [
{
"author": "Г. М. Шевага",
"institution": " Українська науково-дослідна станція карантину рослин ІЗР НААН"
},
{
"author": "М. М. Кирик",
"institution": "Національний університет біоресурсів і природокористування України"
},
{
"author": "В. М. Гунчак",
"institution": " Українська науково-дослідна станція карантину рослин ІЗР НААН"
},
{
"author": "Т. М. Олійник",
"institution": "Інститут картоплярства НААН"
}
] |
| author_sort | Шевага, Г. М. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:42Z |
| description | Results of studies on the optimization of Murashige and Skoog culture medium withmodified maize starch for the acceleration of potato plants micropropogation are provided. Ithas been established that the method modification increases surveillance parameters, promotesactive in vitro growth and development of potato springs. |
| doi_str_mv | 10.35868/1997-3004.24.79-82 |
| first_indexed | 2025-07-17T12:23:10Z |
| format | Article |
| fulltext |
THE OPTIMIZATION OF JELLIFYING AGENT COMPOSITION ON
REGENERATION OF IN VITRO OBTAINED POTATO MICROPLANTS
H. M. Shevaha1, M. M. Kyryk2, V. M. Hunchak1, T. M. Oliinyk3
1Ukrainian Research Station of Plant Quarantine, Institute of Plant Protection, NAAS,
Village of Boiany
2National University of Life and Environmental Sciences of Ukraine, City of Kyiv
3Institut of Potato Planting, NAAS, Urban Settlement of Nemishaieve
Results of studies on the optimization of Murashige and Skoog culture medium with
modified maize starch for the acceleration of potato plants micropropogation are provided. It
has been established that the method modification increases surveillance parameters, promotes
active in vitro growth and development of potato springs.
Key words: in vitro, maize starch, potato starch, wheat starch, culture medium.
In modern conditions of agricultural
manufacture, potato seed production
requires accelerated propagation of the new
varieties of seeds based on high category
seeds. A large reserve for forming high
production seeds the use of in vitro
revitalisation of source material and
application of the different methods of
accelerated propagation. Technology of
potato clonal micropropagation based on
cultivation of mericlonal plants on digest
media in vitro approved oneself in practice.
Artificial digest media for in vitro
cultivation of plant cells, tissues and organs
are multicomponent mixtures containing
mineral and organic compounds of different
chemical nature. They provide cultivation
objects with trophic and physiologically
active substances at the background of
optimal pH potential [1; 2].
Most common, dense digest media
produced based on agar – a mixture of high-
molecular polysaccharides from extracts of
several varieties of red algae, consisting of
two polysaccharides – agarose and
agaropectin, are used in the in vitro culture
of potato. It is used for densification of
liquid digest media, giving them a jelly-like
state. Agar may contain chlorides, sulfates,
ions of calcium, magnesium, and ferrum.
Concentration of impurities varies
depending on the source of sea algae and
method of agar production. Purified agars
that are well suited for tissue culture of
plants are available on the market. Agar is
produced abroad, and as for near-abroad
countries – in the Baltic States and Russia
(only these countries have appropriate raw
material resources for its manufacture
among all near-abroad countries). Cost of
agar imported to Ukraine is very high, and
agaroid produced in Ukraine significantly
differs from agar by chemical composition
and is not suitable for use in the in vitro
culture [3; 4].
In world practice, there are studies
on replacement of agar by other gelling
components, as the cost value of media with
agar substitutes is much lower than agar-
containing. According to
M. T. Upadishevyi, the use of
homopolysaccharides to replace agar
provided increase of propagation factor by
1.2-2.3-fold and sprigging by 1.9-6.9-fold
[5].
Upon clonal micropropagation of
strelitzia (Strelitzia reginae) Brazilian
researchers used such jellifying materials as
agarose, phytogel (Phytagel). The best
results were obtained with phytogel [6; 7].
According to the literature sources,
the use of DDKamod significantly improved
plant regeneration in clonal propagation of
some vegetable cultures [8]. O. V. Bilynskyi
established that agar replacement by
DDKamod has a positive effect on the
development of regenerant plants due to
reduction of their vitrification and stimulates
the production of androgen structures and
regenerant plants in the in vitro culture of
spring barley [9-11].
Given the above, the purpose of our
study was to establish the possibility to use
starch as jellifying component of media
under clonal micropropagation of potato and
assess their effect on the parameters of
survival rate and development of potato
culture in vitro.
Materials and methods. Study on the
selection of new jellifying components was
performed at the Crop Biotechnology
Laboratory of the Ukrainian Scientific
Research Station of Plant Quarantine of the
Plant Protection Institute of the NAAS
during 2015-2016 under sterile conditions
according to the commonly accepted
methods by Kalinin methodology [12; 13].
Micro-cuttings were cultured on Murashige
and Skoog medium [14] based on starches
(maize, potato, wheat) with concentration
from 50 g/L to 150 g/L. Medium with agar
(8 g/L) was used as a control. Concentration
of starches was measured by experimental
method during studies. All digest media
were sterilised by autoclaving under
pressure of 1 atm. during 15-20 min., the
work was carried out in sterile laminar box.
Varieties of potato of Ukrainian selection
were used: Slovianka, Poliska rozheva.
After planting, tube tubes with potato plants
were transferred to the culture room and
grown at air temperature of 22-24 °C,
relative humidity of 60-80 %, illumination
of 4 klx, photoperiod of 16 hours. After
regenerants formed 5-6 nodules, they were
cut again and repeated the process of
regeneration. All experiments were
conducted in three repetitions of 20 plants in
each. During the course of studies, visual
observation of the development of cuttings,
period of rhizogenesis (beginning of the
formation of roots, day) and biometric
measurements of development were
performed. Monitoring and processing of
experimental data was performed every
1.5 months.
Based on the results of conducted
studies, we can conclude that due to the use
of maize starch as jellifying agent instead of
agar, increase of the quality of clonal potato
microplants and cheapening of technology
takes place, since standard technology with
the use of agar spends 8 g of agar priced at
9.12 UAH per 1 L of digest media.
Technology that we had developed, provides
the possibility to use starch, with a spending
of 80 g of starch priced at 1.16 UAH per
1 L. Thus, there is a five-fold cheapening of
the technology through the use of economic
scheme of expenses (9.12 UAH/1.6 UAH =
5.6).
|
| id | oai:ojs2.smic.in.ua:article-119 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:00:50Z |
| publishDate | 2016 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/3e/9879aa900ee8e2ff2700b0a84aba273e.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1192026-07-22T10:10:42Z THE OPTIMIZATION OF JELLIFYING AGENT COMPOSITION ON REGENERATION OF IN VITRO OBTAINED POTATO MICROPLANTS ОПТИМІЗАЦІЯ СКЛАДУ ЖЕЛЮЮЧИХ АГЕНТІВ НА РЕГЕНЕРАЦІЮ МІКРОРОСЛИН КАРТОПЛІ, ОТРИМАНИХ IN VITRO Шевага, Г. М. Кирик, М. М. Гунчак, В. М. Олійник, Т. М. in vitro, maize starch, potato starch, wheat starch, culture medium in vitro, кукурудзяний крохмаль, картопляний крохмаль, пшеничний крохмаль, агар, живильне середовище Results of studies on the optimization of Murashige and Skoog culture medium withmodified maize starch for the acceleration of potato plants micropropogation are provided. Ithas been established that the method modification increases surveillance parameters, promotesactive in vitro growth and development of potato springs. Наведено результати досліджень з оптимізаціїї живильного середовища МурасігеСкуга модифікованого кукурудзяним крохмалем для прискореного клонального мікророзмноження рослин картоплі. Встановлено, що модифікація методу покращує показники приживлюваності, сприяє їх активному росту та розвитку живців картоплі в культурі in vitro. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2016-10-09 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/119 10.35868/1997-3004.24.79-82 Agricultural microbiology; Vol. 24 (2016): Agriciltural microbiology; 79-82 Сільськогосподарська мікробіологія; Том 24 (2016): Сільськогосподарська мікробіологія; 79-82 1997-3004 10.35868/1997-3004.24 en uk https://smic.in.ua/index.php/journal/article/view/119/128 https://smic.in.ua/index.php/journal/article/view/119/129 Авторське право (c) 2016 H. M. Shevaha, M. M. Kyryk, V. M. Hunchak, T. M. Oliinyk https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | in vitro кукурудзяний крохмаль картопляний крохмаль пшеничний крохмаль агар живильне середовище Шевага, Г. М. Кирик, М. М. Гунчак, В. М. Олійник, Т. М. ОПТИМІЗАЦІЯ СКЛАДУ ЖЕЛЮЮЧИХ АГЕНТІВ НА РЕГЕНЕРАЦІЮ МІКРОРОСЛИН КАРТОПЛІ, ОТРИМАНИХ IN VITRO |
| title | ОПТИМІЗАЦІЯ СКЛАДУ ЖЕЛЮЮЧИХ АГЕНТІВ НА РЕГЕНЕРАЦІЮ МІКРОРОСЛИН КАРТОПЛІ, ОТРИМАНИХ IN VITRO |
| title_alt | THE OPTIMIZATION OF JELLIFYING AGENT COMPOSITION ON REGENERATION OF IN VITRO OBTAINED POTATO MICROPLANTS |
| title_full | ОПТИМІЗАЦІЯ СКЛАДУ ЖЕЛЮЮЧИХ АГЕНТІВ НА РЕГЕНЕРАЦІЮ МІКРОРОСЛИН КАРТОПЛІ, ОТРИМАНИХ IN VITRO |
| title_fullStr | ОПТИМІЗАЦІЯ СКЛАДУ ЖЕЛЮЮЧИХ АГЕНТІВ НА РЕГЕНЕРАЦІЮ МІКРОРОСЛИН КАРТОПЛІ, ОТРИМАНИХ IN VITRO |
| title_full_unstemmed | ОПТИМІЗАЦІЯ СКЛАДУ ЖЕЛЮЮЧИХ АГЕНТІВ НА РЕГЕНЕРАЦІЮ МІКРОРОСЛИН КАРТОПЛІ, ОТРИМАНИХ IN VITRO |
| title_short | ОПТИМІЗАЦІЯ СКЛАДУ ЖЕЛЮЮЧИХ АГЕНТІВ НА РЕГЕНЕРАЦІЮ МІКРОРОСЛИН КАРТОПЛІ, ОТРИМАНИХ IN VITRO |
| title_sort | оптимізація складу желюючих агентів на регенерацію мікророслин картоплі, отриманих in vitro |
| topic | in vitro кукурудзяний крохмаль картопляний крохмаль пшеничний крохмаль агар живильне середовище |
| topic_facet | in vitro maize starch potato starch wheat starch culture medium in vitro кукурудзяний крохмаль картопляний крохмаль пшеничний крохмаль агар живильне середовище |
| url | https://smic.in.ua/index.php/journal/article/view/119 |
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