КОРЕГУЮЧИЙ ВПЛИВ БІОМАСИ КАРОТИНОСИНТЕЗУВАЛЬНИХ ДРІЖДЖІВ PHAFFIA RHODOZYMA НА ФОРМУВАННЯ МІКРОБОЦЕНОЗУ КИШКОВИКА КАЧОК
The changes the of intestinal microflora composition of Beijing broiler ducks at introduction of caroteneproduced yeast Phaffia rhodozyma biomass to the diet was studied. The positive changes of 37-days ducks intestinal microflora were revealed after two weeks of yeast P. rhodozyma biomasses applica...
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| Date: | 2016 |
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| Language: | English Ukrainian |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2016
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| Online Access: | https://smic.in.ua/index.php/journal/article/view/125 |
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| Journal Title: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465392743907328 |
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| author | Камінська, М. В. Стефанишин, О. М. Гунчак, А. В. |
| author_facet | Камінська, М. В. Стефанишин, О. М. Гунчак, А. В. |
| author_institution_txt_mv | [
{
"author": "М. В. Камінська",
"institution": "Інститут біології тварин НААН"
},
{
"author": "О. М. Стефанишин",
"institution": "Інститут біології тварин НААН"
},
{
"author": "А. В. Гунчак",
"institution": "Інститут біології тварин НААН"
}
] |
| author_sort | Камінська, М. В. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:42Z |
| description | The changes the of intestinal microflora composition of Beijing broiler ducks at introduction of caroteneproduced yeast Phaffia rhodozyma biomass to the diet was studied. The positive changes of 37-days ducks intestinal microflora were revealed after two weeks of yeast P. rhodozyma biomasses application in 6- day-old birds’ diet (1 % of the weight of feed). It was a decrease of the E. coli total number and pathogenic staphylococci strains, the absence of Enterobacteriaceae (lac–) strains against the high number of bifidobacteria and lactobacilli. The E. coli total number reduced by 10 times at the expense of E. coli (lac±)-strains compared to 37-day birds three weeks after the experiment. The staphylococcus non-pathogenic strains number in 58-days ducks experimental group caecum contents reduced compared to 37-day birds three weeks after the experiment. Thus, the using of caroteneproduced yeast P. rhodozyma biomass in poultry diets helps to prevent violations of intestinal microflora and occurrence dysbiosis at critical periods of growth and development. |
| doi_str_mv | 10.35868/1997-3004.23.30-35 |
| first_indexed | 2025-07-17T12:23:14Z |
| format | Article |
| fulltext |
THE CORRECTIVE EFFECT OF CAROTENEPRODUCED YEAST
PHAFFIA RHODOZYMA BIOMASS ON INTESTINAL MICROBIOCENOSIS FORMATION OF
DUCKS
M. V. Kaminska, О. М. Stefanyshyn, А. V. Hunchak
During husbandry of the numerous groups of poultry on one territory, infectious agents are
accumulated and activated in population. It is known that the environmental microflora influences the
intestinal microbiocenosis, especially in the first days of life of young poultry. Further, condition of
intestinal microflora influences metabolism and health of poultry. In case of impairment of intestinal
microflora, it is necessary to carry out therapeutic measures. To control pathogenic microorganisms,
antibiotics that inhibit the development of not only pathogens but also normal intestinal microflora
leading to dysbacteriosis are used. World experience with antibiotics has shown that there is no need to
use any preventive control measures to destroy pathogenic microflora. Prevention and treatment of
diseases caused by opportunistic and pathogenic microorganisms should be carried out stimulating natural
resistance of microorganism. One of the promising areas in this is the use of probiotics.
In terms of efficiency of action, probiotics is not inferior to some antibiotics and chemotherapeutic
agents. At the same time they have no negative influence on the microflora of the digestive tract, do not
pollute livestock products and environment, meaning they are environmentally friendly. The use of
probiotics is safe for people who consume livestock products. Probiotics not only normalize the
composition of intestinal microflora, but also increase the productivity of animals. Our previous studies
have shown that the use of yeasts under dysbiosis in rats positively influences the intestinal microflora
composition and metabolism of animals. Analysis of the literature data shows that yeast cells have
complex mechanisms of influence on intestinal microflora and the state of the microorganism. In
particular, they prevent colonization of the gastrointestinal tract by pathogens, stimulate growth of
endogenous lacto- and bifidobacteria, adsorb mycotoxins, change the redox potential of near-epithelial
layer of the intestine, increase enzymatic activity of the digestive tract, synthesis of lactase, maltase and
saccharase. Fragments of the yeast cell wall stimulate a weak immune system of young animals and
poultry. They modulate the activity of cells of the immune system, increasing body resistance to
infections.
Therefore, the purpose of this study was to investigate the possibility of using biomass of
carotene-synthesizing yeasts Phaffia rhodozyma for the correction of intestinal microflora composition in
critical periods of growth and development of poultry, in particular ducks.
Materials and methods. To implement settled task, experiment under conditions of PE “Lisnyi”
of the District of Pustomyty of the Region of Lviv in Beijing broiler ducks of cross STAR 53 (heavy),
selection of French breeding company Grimaud Freres Selection was carried out. Husbandry of poultry
was the floor with free access to feed and water, according to current technological requirements. All
poultry received complete feed, balanced in nutrients and bioactive substances, according to the direction
of productivity and growing period. Two groups of poultry were formed: control and experimental, with
15 birds in each. Ducks of the experimental group, starting from 6-day age, receive 1 % of biomass of
carotene-synthesizing yeasts P. rhodozyma, strain KNH1 during 14 days. Composition of intestinal
microflora at the age of 37 and 58 days was studied in young and adult ducks. At the end of the specified
age periods, 5–6 birds were slaughtered. Content of the blind and small intestine of poultry was the study
material. Samples of intestinal content were selected after slaughter and transferred into the sterile test
tubes. Quantitative and qualitative composition of microflora using dilution method and inoculation of
microorganisms on elective media (Endo, Sabouraud, bismuth-sulphite, Baird-Parker, Blaurock, blood
agar) was studied in the samples of the intestinal flora.
Their identification was carried out by morphological, cultural, physiological, and biochemical
properties (Olkenytskyi and Simons media).
Statistical processing of the results was performed by Microsoft Excel, using the Student test.
Thus, after two weeks of use of biomass of carotene-synthesizing yeasts Phaffia rhodozyma (1 %
of the feed mass) in the diet of ducks at the age of 6 days, content of ducks at the age of 37 days in the
experimental group showed positive changes in the composition of microflora. For example, slight
decrease in the total count of cells of E. coli without redistribution of strain ratio with different enzymatic
capability and lack of lactose-negative strains was established. Total count of bifidobacteria and
lactobacilli was 99 % from the total count of microorganisms (1010 CFU/g). Three weeks after the
experiment termination, content of the blind intestine of the ducks at the age of 58 days in the
experimental group showed 10-fold reduction of the total count of cells of E. coli due to low-fermenting
strains of E. coli, which changed the ratio between strains with different fermentative capability lac+ to
lac± from 99.9 : 0.1 (37-day ducks) to 93 : 7 (58-day ducks). Reduction of the total count of non-
pathogenic strains of staphylococci at the background of high count of bifidobacteria and lactobacilli was
established.
Therefore, the use of biomass of carotene-synthesizing yeasts Phaffia rhodozyma in the diets of
ducks in critical periods of their growth and development allows to prevent impairment of composition of
intestinal microflora and occurrence of dysbiosis.
|
| id | oai:ojs2.smic.in.ua:article-125 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:01:19Z |
| publishDate | 2016 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/96/32bbb5dc89c87547807a0bdb1fb9f196.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1252026-07-22T10:10:42Z THE CORRECTIVE EFFECT OF CAROTENEPRODUCED YEAST PHAFFIA RHODOZYMA BIOMASS ON INTESTINAL MICROBIOCENOSIS FORMATION OF DUCKS КОРЕГУЮЧИЙ ВПЛИВ БІОМАСИ КАРОТИНОСИНТЕЗУВАЛЬНИХ ДРІЖДЖІВ PHAFFIA RHODOZYMA НА ФОРМУВАННЯ МІКРОБОЦЕНОЗУ КИШКОВИКА КАЧОК Камінська, М. В. Стефанишин, О. М. Гунчак, А. В. microflora, intestine, ducks мікрофлора, Phaffia rhodozyma, кишковик, качки The changes the of intestinal microflora composition of Beijing broiler ducks at introduction of caroteneproduced yeast Phaffia rhodozyma biomass to the diet was studied. The positive changes of 37-days ducks intestinal microflora were revealed after two weeks of yeast P. rhodozyma biomasses application in 6- day-old birds’ diet (1 % of the weight of feed). It was a decrease of the E. coli total number and pathogenic staphylococci strains, the absence of Enterobacteriaceae (lac–) strains against the high number of bifidobacteria and lactobacilli. The E. coli total number reduced by 10 times at the expense of E. coli (lac±)-strains compared to 37-day birds three weeks after the experiment. The staphylococcus non-pathogenic strains number in 58-days ducks experimental group caecum contents reduced compared to 37-day birds three weeks after the experiment. Thus, the using of caroteneproduced yeast P. rhodozyma biomass in poultry diets helps to prevent violations of intestinal microflora and occurrence dysbiosis at critical periods of growth and development. Вивчено зміни у складі мікрофлори кишковика пекінських бройлерних качок за введення у раціон біомаси каротиносинтезувальних дріжджів Phaffia rhodozyma. Після двотижневого застосування у раціоні качок з 6-денного віку біомаси дріжджів P. rhodozyma (1 % від маси корму) виявлено позитивні зміни у складі мікрофлори кишковика 37-добової птиці: зменшення загальної кількості клітин кишкової палички та непатогенних штамів стафілококів, відсутність лактозонегативних ентеробактерій на фоні високої кількості біфідобактерій і лактобактерій. Через три тижні після завершення досліду у вмісті сліпих кишок 58-добових качок дослідної групи зменшилася загальна кількість клітин кишкової палички у 10 разів за рахунок слабоферментуючих штамів E. coli та чисельність непатогенних штамів стафілококів, порівняно до показників у 37-добової птиці. Отже, застосування біомаси каротиносинтезувальних дріжджів P. rhodozyma у раціонах сільськогосподарської птиці у критичні періоди росту та розвитку дозволяє попередити порушення складу мікрофлори кишковика та виникнення дисбактеріозу. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2016-06-29 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/125 10.35868/1997-3004.23.30-35 Agricultural microbiology; Vol. 23 (2016): Agriciltural microbiology; 30-35 Сільськогосподарська мікробіологія; Том 23 (2016): Сільськогосподарська мікробіологія; 30-35 1997-3004 10.35868/1997-3004.23 en uk https://smic.in.ua/index.php/journal/article/view/125/138 https://smic.in.ua/index.php/journal/article/view/125/139 Авторське право (c) 2016 M. V. Kaminska, О. М. Stefanyshyn, A. V. Guntchak https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | мікрофлора Phaffia rhodozyma кишковик качки Камінська, М. В. Стефанишин, О. М. Гунчак, А. В. КОРЕГУЮЧИЙ ВПЛИВ БІОМАСИ КАРОТИНОСИНТЕЗУВАЛЬНИХ ДРІЖДЖІВ PHAFFIA RHODOZYMA НА ФОРМУВАННЯ МІКРОБОЦЕНОЗУ КИШКОВИКА КАЧОК |
| title | КОРЕГУЮЧИЙ ВПЛИВ БІОМАСИ КАРОТИНОСИНТЕЗУВАЛЬНИХ ДРІЖДЖІВ PHAFFIA RHODOZYMA НА ФОРМУВАННЯ МІКРОБОЦЕНОЗУ КИШКОВИКА КАЧОК |
| title_alt | THE CORRECTIVE EFFECT OF CAROTENEPRODUCED YEAST PHAFFIA RHODOZYMA BIOMASS ON INTESTINAL MICROBIOCENOSIS FORMATION OF DUCKS |
| title_full | КОРЕГУЮЧИЙ ВПЛИВ БІОМАСИ КАРОТИНОСИНТЕЗУВАЛЬНИХ ДРІЖДЖІВ PHAFFIA RHODOZYMA НА ФОРМУВАННЯ МІКРОБОЦЕНОЗУ КИШКОВИКА КАЧОК |
| title_fullStr | КОРЕГУЮЧИЙ ВПЛИВ БІОМАСИ КАРОТИНОСИНТЕЗУВАЛЬНИХ ДРІЖДЖІВ PHAFFIA RHODOZYMA НА ФОРМУВАННЯ МІКРОБОЦЕНОЗУ КИШКОВИКА КАЧОК |
| title_full_unstemmed | КОРЕГУЮЧИЙ ВПЛИВ БІОМАСИ КАРОТИНОСИНТЕЗУВАЛЬНИХ ДРІЖДЖІВ PHAFFIA RHODOZYMA НА ФОРМУВАННЯ МІКРОБОЦЕНОЗУ КИШКОВИКА КАЧОК |
| title_short | КОРЕГУЮЧИЙ ВПЛИВ БІОМАСИ КАРОТИНОСИНТЕЗУВАЛЬНИХ ДРІЖДЖІВ PHAFFIA RHODOZYMA НА ФОРМУВАННЯ МІКРОБОЦЕНОЗУ КИШКОВИКА КАЧОК |
| title_sort | корегуючий вплив біомаси каротиносинтезувальних дріжджів phaffia rhodozyma на формування мікробоценозу кишковика качок |
| topic | мікрофлора Phaffia rhodozyma кишковик качки |
| topic_facet | microflora intestine ducks мікрофлора Phaffia rhodozyma кишковик качки |
| url | https://smic.in.ua/index.php/journal/article/view/125 |
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