ФІТОВІРУСОЛОГІЧНИЙ МОНІТОРИНГ НАСАДЖЕНЬ КАРТОПЛІ В АГРОЦЕНОЗАХ ЧЕРНІГІВСЬКОЇ ОБЛАСТІ
The results of monitoring researches of potato virus diseases in the soil and climatic conditions of the Chernigov region. An analysis of the situation in the potato seed shows that the variety is largely defeat viral diseases. In potato seed plots found: potato M-, S-, Y-virus, in monoinfection, an...
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| Date: | 2016 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English Ukrainian |
| Published: |
Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2016
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| Online Access: | https://smic.in.ua/index.php/journal/article/view/126 |
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| Journal Title: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465394656509952 |
|---|---|
| author | Дмитрук, О. О. Дерев’янко, С. В. Решотько, Л. М. Волкова, І. В. Кучерявенко, О. О. Бова, Т. О. Чженьхуа, Чжан |
| author_facet | Дмитрук, О. О. Дерев’янко, С. В. Решотько, Л. М. Волкова, І. В. Кучерявенко, О. О. Бова, Т. О. Чженьхуа, Чжан |
| author_institution_txt_mv | [
{
"author": "О. О. Дмитрук",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "С. В. Дерев’янко",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "Л. М. Решотько",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "І. В. Волкова",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "О. О. Кучерявенко",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "Т. О. Бова",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "Чжан Чженьхуа",
"institution": "Китайсько-українська аграрна компанія «Фанда»"
}
] |
| author_sort | Дмитрук, О. О. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:42Z |
| description | The results of monitoring researches of potato virus diseases in the soil and climatic conditions of the Chernigov region. An analysis of the situation in the potato seed shows that the variety is largely defeat viral diseases. In potato seed plots found: potato M-, S-, Y-virus, in monoinfection, and as part of pathocomplexes. Prevails in crops entomophilous potato M virus as monoinfection (36 %) or in combination with other mosaic viruses. Potato virus Y is found in 18 from 47 potato cultivars. A study of the species composition of pathogens and their vectors, infection pathways in the field and deve lopment of viral disease, is the basis for the proper and effective application of safeguard measures. |
| doi_str_mv | 10.35868/1997-3004.23.36-41 |
| first_indexed | 2025-07-17T12:23:15Z |
| format | Article |
| fulltext |
PHYTOVIRUS MONITORING AT THE POTATO FIELDS IN CHERNIHIV REGION
AGROCENOSES
О. О. Dmytruk, S. V. Derevianko, L. M. Reshotko, I. V. Volkova, О. О. Kucheriavenko,
Т. О. Bova, Zhang Zhenghua
Among various diseases of potatoes viral ones are widespread with a tendency towards
increasing of their harm in the main regions of potato cultivation in many varieties that are in
economic and trade turnover. Fifty three viruses of 22 families which differ in morphological,
physical, biological and immunological properties have been described in the culture; about 30
of which are important in the global potato farming. However, the species composition of
pathogens and the degree of infestation of planting vary depending on natural and economic
conditions, varieties of potato and state of seed farming.
Under natural conditions of Ukraine potato virus M, potato virus Y, potato virus S, potato
virus X, potato leafroll virus are widespread. Enrichment of population with new to the region
harmful viruses and their strains was established. Along with common ones, necrotic strains of
potato virus Y become more widespread; strain causing the formation of the bulb ring necrosis
(PVYNTN) was found. Pathogens, carriers of which are ground parasitic nematodes and fungi that
cause damage to the bulbs, which reduces their marketability and suitability for food
consumption and industrial processing: tobacco rattle virus (TRV), potato mop top virus
(PMTV), potato spindle tuber viroid (PSTV) were identified.
Unlike other pathogens, virus in the infected the plant remains throughout its life, and
also in its vegetative progeny that results in the accumulation of viruses in crops and seeds, as
well as agrocenoses. Under the scheme of manufacturing of certified seed potatoes of the
European Plant Protection Organization, the following viruses has been specified for testing:
basic potato virus A, M, S, X, Y, potato leafroll virus (PLV) and others – alfalfa mosaic virus
(AMV) , cucumber mosaic virus (CMV), potato aucuba mosaic virus (PAMV), PMTV, tobacco
mosaic virus (TMV), TRV, tomato black ringspot virus (TBRV), tomato spotted wilt virus
(TSWV) [3].
Harm of viral diseases of plants appears to reduce the yield of plants, deteriorate product
marketability and quality. Yield losses due to viral lesions depend on the species and strains of
pathogens common in this soil and climatic zone. Yield losses of potatoes in Polissia of Ukraine
due to viral diseases are on average 30–40 %, reaching 38–70 %. Lesions from the virus M in
Polissia may constitute more than 40 % of the crop, growing under complex involvement of
plants with several pathogens. In this zone, most of varieties are completely infected with virus
M, and therefore the economy annually fails to receive much of the harvest. Most viruses are
transmitted to bulb offspring tuberous with the efficiency of 100 %, excluding tobacco rattle
virus and potato mop top virus — involvement of bulbs with these viruses from diseased plants
ranges from 20 % to 85 %.
Performance of potato farming is determined, above all, by the perfection of infection
control system used in seed farming. It is shown that propagation of recovered material in
conditions that do not provide complete protection of plants against viral reinfection leads to
rapid accumulation of viruses and reduction of varietal and sowing qualities of seed potatoes.
The rate of growth of viral infection is largely determined by the susceptibility of varieties to
certain viruses and their complexes as well as by the level of infection load in the manufacturing
places.
Thus, the urgency of phytoviruses prevalence in the environment has both fundamental
and practical value. Knowledge of species composition of pathogens and their vectors in a
particular area, pathways of infection spreading in the field, factors that contribute to the
involvement of plants and manifestation of disease signs or are limiting factors for infection,
allows to predict the emergence and development of viral diseases and is the basis for proper
selection and effective application of protective measures.
Materials and methods. A survey of potato planting was conducted according to the
current guidelines. Accounting and sampling for laboratory analysis was conducted in budding to
flowering phase of potato plants using diagonal sampling method. For virological study,
3–4 leaves of the upper and middle layer were sampled from the main stem of the plant and
stored at 4 °C. The leaves were sampled not only from plants that have external signs of disease,
but also from healthy ones, given the possibility of latent virus carriage.
Study results were expressed by the parameter of disease prevalence that was calculated
by the equation:
100
N
nР ,
where P — prevalence of the disease, %;
n — number of involved plants in the sample, units;
N — total number of plants in the sample, units.
For the detection and identification of potato viruses, method of visual and serological
diagnostic, electron microscopy of native preparations (EM), bioassay were used. Antisera for
detection of potato viruses obtained in the Laboratory of Virology of the Institute of Agricultural
Microbiology and Agroindustrial Manufacture of NAAS were used for serological analyses.
Native preparations for electron microscopy were prepared by negative staining method
modified in the Laboratory of Virology of the IAMAM. Preparations were studied in electron
microscopes Tesla-540 (Czech Republic) and EM-125 (Ukraine) under instrumental
magnification ×20–22 thousand.
To clarify the nature of the disease and composition of pathological complexes under
viral infection, the method of indicator plants was used. Using mechanical inoculation, with
previous application of silicon carbide, virus was passed to the tobacco Nicotiana tabacum L.
Tobacco and tomatoes Lycopersicon esculentum Mill. Test plants that remained asymptomatic
carriers of the virus or showed symptoms of infection, local or systemic. In all the experiments,
healthy not inoculated plants were used as the control. Plants were grown under conditions of
growing room at a temperature of 20–22 °C with 16 hours photoperiod. Within 14-30 days after
inoculation, development of symptoms was followed-up, and viral infection was controlled by
immunological and electron microscopy methods.
Thus, agrocenoses of the Chernihiv region show intensive infection of potato plants with
viruses, which suggests hard natural infectious background (presence of effective rezervators and
vectors of potato viruses in the environment). When growing a healthy source material in the
field during 2–3 years, there is a high degree of prevalence of mosaic group viruses. Therefore,
determination of the risks of prevalence of phytopathogenic potato viruses based on investigation
of the environmental features of the pathogen are needed for effective control of viral diseases at
all stages of growing varieties (in the process of recovery, in the manufacture of seed material,
its certification, carrying out assessment of selective samples). For this purpose it is required to
use not only visual assessment of crops but also to use laboratory methods for diagnostics of
potato viruses.
In order to improve the level of phytosanitary safety and obtain high-quality seed
material, it is required to carry out primary seed farming in the protected ground and use
recovered varieties of potatoes and meet effective methods of protecting plants against viruses.
When growing potatoes in the field, it is required to use recovered seed potatoes and follow
technology that ensures avoidance of viral transmission from rezervators.
|
| id | oai:ojs2.smic.in.ua:article-126 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:01:20Z |
| publishDate | 2016 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/d4/784e49f273a12a58f7f39627c2cadfd4.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1262026-07-22T10:10:42Z PHYTOVIRUS MONITORING AT THE POTATO FIELDS IN CHERNIHIV REGION AGROCENOSES ФІТОВІРУСОЛОГІЧНИЙ МОНІТОРИНГ НАСАДЖЕНЬ КАРТОПЛІ В АГРОЦЕНОЗАХ ЧЕРНІГІВСЬКОЇ ОБЛАСТІ Дмитрук, О. О. Дерев’янко, С. В. Решотько, Л. М. Волкова, І. В. Кучерявенко, О. О. Бова, Т. О. Чженьхуа, Чжан phytovirus monitoring, potato virus disease, virus strain фітовірусологічний моніторинг, вірусні хвороби картоплі, вірусні ізоляти The results of monitoring researches of potato virus diseases in the soil and climatic conditions of the Chernigov region. An analysis of the situation in the potato seed shows that the variety is largely defeat viral diseases. In potato seed plots found: potato M-, S-, Y-virus, in monoinfection, and as part of pathocomplexes. Prevails in crops entomophilous potato M virus as monoinfection (36 %) or in combination with other mosaic viruses. Potato virus Y is found in 18 from 47 potato cultivars. A study of the species composition of pathogens and their vectors, infection pathways in the field and deve lopment of viral disease, is the basis for the proper and effective application of safeguard measures. Представлено результати моніторингових досліджень вірусних хвороб картоплі у ѓрунтово-клiматичних умовах Чернігівської області. Аналіз ситуації, яка склалася у насінництві картоплі, показує, що сорти значною мірою уражені вірусними хворобами. У розсадниках елітного насінництва картоплі виявлено М-, S-, Y-віруси картоплі, як у моноінфекції, так і у складі патокомплексів. Превалює в посівах ентомофільний М-вірус картоплі в моноінфекції (36 %) або у комплексі з іншими мозаїчними вірусами. Y-вірус картоплі виявлено у рослинах 18 з проаналізованих 47 сортів картоплі. Дослідження видового складу збудників, їх векторів, шляхів поширення інфекцій у польових умовах дає змогу прогнозувати появу та розвиток вірусних хвороб і є основою для правильного та ефективного застосування захисних заходів. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2016-06-29 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/126 10.35868/1997-3004.23.36-41 Agricultural microbiology; Vol. 23 (2016): Agriciltural microbiology; 36-41 Сільськогосподарська мікробіологія; Том 23 (2016): Сільськогосподарська мікробіологія; 36-41 1997-3004 10.35868/1997-3004.23 en uk https://smic.in.ua/index.php/journal/article/view/126/140 https://smic.in.ua/index.php/journal/article/view/126/141 Авторське право (c) 2016 O. O. Dmitruk, S. V. Derevianko, L. M. Reshotko, I. V. Volkova, О. О. Kucheriavenko, Т. О. Bova, Zhang Zhenghua https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | фітовірусологічний моніторинг вірусні хвороби картоплі вірусні ізоляти Дмитрук, О. О. Дерев’янко, С. В. Решотько, Л. М. Волкова, І. В. Кучерявенко, О. О. Бова, Т. О. Чженьхуа, Чжан ФІТОВІРУСОЛОГІЧНИЙ МОНІТОРИНГ НАСАДЖЕНЬ КАРТОПЛІ В АГРОЦЕНОЗАХ ЧЕРНІГІВСЬКОЇ ОБЛАСТІ |
| title | ФІТОВІРУСОЛОГІЧНИЙ МОНІТОРИНГ НАСАДЖЕНЬ КАРТОПЛІ В АГРОЦЕНОЗАХ ЧЕРНІГІВСЬКОЇ ОБЛАСТІ |
| title_alt | PHYTOVIRUS MONITORING AT THE POTATO FIELDS IN CHERNIHIV REGION AGROCENOSES |
| title_full | ФІТОВІРУСОЛОГІЧНИЙ МОНІТОРИНГ НАСАДЖЕНЬ КАРТОПЛІ В АГРОЦЕНОЗАХ ЧЕРНІГІВСЬКОЇ ОБЛАСТІ |
| title_fullStr | ФІТОВІРУСОЛОГІЧНИЙ МОНІТОРИНГ НАСАДЖЕНЬ КАРТОПЛІ В АГРОЦЕНОЗАХ ЧЕРНІГІВСЬКОЇ ОБЛАСТІ |
| title_full_unstemmed | ФІТОВІРУСОЛОГІЧНИЙ МОНІТОРИНГ НАСАДЖЕНЬ КАРТОПЛІ В АГРОЦЕНОЗАХ ЧЕРНІГІВСЬКОЇ ОБЛАСТІ |
| title_short | ФІТОВІРУСОЛОГІЧНИЙ МОНІТОРИНГ НАСАДЖЕНЬ КАРТОПЛІ В АГРОЦЕНОЗАХ ЧЕРНІГІВСЬКОЇ ОБЛАСТІ |
| title_sort | фітовірусологічний моніторинг насаджень картоплі в агроценозах чернігівської області |
| topic | фітовірусологічний моніторинг вірусні хвороби картоплі вірусні ізоляти |
| topic_facet | phytovirus monitoring potato virus disease virus strain фітовірусологічний моніторинг вірусні хвороби картоплі вірусні ізоляти |
| url | https://smic.in.ua/index.php/journal/article/view/126 |
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