СИНТЕЗ ЕТИЛЕНУ ФІТОПАТОГЕННИМ ГРИБОМ ACREMONIUM SP. 502
The ability of Acremonium sp. 502 phytopathogenic fungi to produce ethylene was studied.It was found that Acremonium sp. 502 is able tosynthesize ethylene. The highest level of biosynthesis was recorded after 5 weeks of cultivationthat coincides with the terms of the highest levelof synthesis of cel...
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| Datum: | 2015 |
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| Format: | Artikel |
| Sprache: | Englisch Ukrainisch |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2015
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| Online Zugang: | https://smic.in.ua/index.php/journal/article/view/130 |
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| Назва журналу: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465402032193536 |
|---|---|
| author | Цехмістер, Г. В. |
| author_facet | Цехмістер, Г. В. |
| author_institution_txt_mv | [
{
"author": "Г. В. Цехмістер",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Цехмістер, Г. В. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:42Z |
| description | The ability of Acremonium sp. 502 phytopathogenic fungi to produce ethylene was studied.It was found that Acremonium sp. 502 is able tosynthesize ethylene. The highest level of biosynthesis was recorded after 5 weeks of cultivationthat coincides with the terms of the highest levelof synthesis of cellulases that are involved in thepenetration of the fungus into the plant. A suggestion that ethylene synthesis plays a certainrole in the mechanism of pathogenesis of cucumbers plants is offered. |
| doi_str_mv | 10.35868/1997-3004.22.9-12 |
| first_indexed | 2025-07-17T12:23:17Z |
| format | Article |
| fulltext |
SYNTHESIS OF ETHYLENE WITH ACREMONIUM SP. 502
PHYTOPATHOGENIC FUNGI
G. V. Tsehmister
Representatives of Cucurbitaceae family
are among the most common types of vegetables
grown in Ukraine. The problem of their
destruction with fungi in Ukraine has not been
investigated enough. Besides, pathogenic fungi
lead to considerable losses in agriculture.
Representatives of Acremonium Link genus,
mostly lead saprotrophic way of nutrition, but in
certain circumstances they can change
specialization and cause diseases of plants of
Cucurbitaceae family, manifesting themselves as
facultative parasites. Fungi of Acremonium genus
were isolated from diseased plants of melons in
Spain, Italy and the US (California and Texas)
[13-18; 20]. In 2011, S.P. Nadkernychnyi
separated strain of Acremonium sp. 502 from
cucumber plants were grown under conditions of
closed ground and had disease symptoms. We
have confirmed its pathogenicity on cucumber
plants of Koroliok variety. It was revealed that it
is localized in the root system, root neck and
hypocotyl and the disease starts to manifest itself
in the phase of true leaves. [12]
Phytopathogenic microorganisms produce a
number of secondary metabolites that negatively
affect the growth and development of plants. We
know that the ability to synthesize
phytohormones often play an important role in
the pathogenesis and correlates with virulence
and specialization of pathogen. In case of
obligate parasitism the use of host plant
regulatory systems by pathogen is an important
combination factor. Phytohormones were
detected in spores, culture liquid and mycelium
of many pathogenic fungi. It is believed that the
intensive production of hormones is associated
with the implementation of phytopathogenic
features such as tissue necrosis and their
hydrolysis into simple compounds [2], it is also
known that hypersynthesis of phytohormones
unbalances hormonal system of plants and is the
cause of many diseases [7]. The determination of
fungi ability to produce phytohormonal
substances enables to determine the mechanism
of their influence on plant organism. One of the
components of fungi pathogenesis is ethylene
production [4].
Ethylene is a natural plant growth regulator,
one of the main phytohormones. In the plant it
controls a wide range of physiological processes,
ripening of fruit and aging of tissues, seed
germination, growth of cells by stretching, as
well as it participates in plants response to
various stressors. Typical processes that are
activated by ethylene are accelerating leaves
aging [9], stunting of stem growth in length, its
thickening and horizontal growth [1]. At the
same time ethylene can reduce polar auxin
transportation, inhibit cell division, accelerate
fruit falling, cause flowers aging, stun roots
growth [19]. It is known that fungi of Fusarium,
Penicillium, Verticillium, Mucor,
Saccharomyces genera are able to synthesize
ethylene [9].
In view of the above, the objective of our
study was to investigate the ability of
Acremonium sp. 502 to produce ethylene as one
of the mechanisms of influence on plants.
Materials and methods. Pathogenic strain
of fungi of Acremonium genus, was studied,
which was isolated from diseased cucumber
plants that were grown in the conditions of closed
ground. Strain virulence was preapproved on
cucumbers of Koroliok variety. The culture of
fungus was kept on slant wort-agar (4 Balling
degrees). The description of morphological and
cultural characteristics was presented previously
by us [10].
To study the synthesis of ethylene
Acremonium sp. 502 was superficially cultured in
glass vials on Chapek synthetic medium at the
temperature of 26-28°C. 10 ml of medium were
poured on into 30 ml vials. Acremonium sp. 502
seeding material was obtained by flushing
conidia and fungus hyphae pieces from slant
wort-agar. Sowing was carried with spore
suspension (T = 1 x 106 CFU) in the amount of
5% from the volume of culture medium.
Measurement of ethylene was performed every 7
days over 7 weeks. At this vials were sealed for a
day. The composition of air in the gas phase was
analyzed on gas chromatograph Chrom-4 [4].
The composition of air in vials without fungus
inoculums served as a control. Fungus mycelium
was separated through filter paper, washed
several times with distilled water and dried to
constant weight at 105°C. The calculation of
ethylene in the sample was done according to the
proposed method [6].
As the criterion for assessing of observed
changes probability standard deviation was
calculated [5].
Thus, we found that pathogenic to cucumber
plants Acremonium sp. 502 fungus is able to
produce ethylene. The highest level of
biosynthesis was recorded after 5 weeks of
cultivation, coinciding with the terms of the
highest level of cellulases synthesis that are
involved in the penetration of the fungus into the
plant. We can therefore assume that ethylene
synthesis plays a certain role in the mechanism of
pathogenesis of cucumber plants by influencing
the activity of cellulases and accelerating leaves
aging.
|
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| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:01:27Z |
| publishDate | 2015 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/66/82127b8fbb41fefe3bcd4ef20a1ba266.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1302026-07-22T10:10:42Z SYNTHESIS OF ETHYLENE WITH ACREMONIUM SP. 502 PHYTOPATHOGENIC FUNGI СИНТЕЗ ЕТИЛЕНУ ФІТОПАТОГЕННИМ ГРИБОМ ACREMONIUM SP. 502 Цехмістер, Г. В. ethylene, phytopathogen, Acremonium етилен, фітопатоген, Acremonium The ability of Acremonium sp. 502 phytopathogenic fungi to produce ethylene was studied.It was found that Acremonium sp. 502 is able tosynthesize ethylene. The highest level of biosynthesis was recorded after 5 weeks of cultivationthat coincides with the terms of the highest levelof synthesis of cellulases that are involved in thepenetration of the fungus into the plant. A suggestion that ethylene synthesis plays a certainrole in the mechanism of pathogenesis of cucumbers plants is offered. Вивчено здатність фітопатогенного гриба Acremonium sp. 502 продукувати етилен in vitro. Встановлено, що Acremonium sp. 502 здатний до синтезу етилену. Найвищий рівень біосинтезу зафіксовано через 5 тижнів культивування, що співпадає зі строками найвищого рівня синтезу целюлаз, які беруть участь у проникненні гриба в рослину. Висувається припущення, що синтез етилену відіграє певну роль у механізмі патогенезу рослин огірків. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2015-12-29 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/130 10.35868/1997-3004.22.9-12 Agricultural microbiology; Vol. 22 (2015): Agriciltural microbiology; 9-12 Сільськогосподарська мікробіологія; Том 22 (2015): Сільськогосподарська мікробіологія; 9-12 1997-3004 10.35868/1997-3004.22 en uk https://smic.in.ua/index.php/journal/article/view/130/146 https://smic.in.ua/index.php/journal/article/view/130/147 Авторське право (c) 2015 G. V. Tsehmister https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | етилен фітопатоген Acremonium Цехмістер, Г. В. СИНТЕЗ ЕТИЛЕНУ ФІТОПАТОГЕННИМ ГРИБОМ ACREMONIUM SP. 502 |
| title | СИНТЕЗ ЕТИЛЕНУ ФІТОПАТОГЕННИМ ГРИБОМ ACREMONIUM SP. 502 |
| title_alt | SYNTHESIS OF ETHYLENE WITH ACREMONIUM SP. 502 PHYTOPATHOGENIC FUNGI |
| title_full | СИНТЕЗ ЕТИЛЕНУ ФІТОПАТОГЕННИМ ГРИБОМ ACREMONIUM SP. 502 |
| title_fullStr | СИНТЕЗ ЕТИЛЕНУ ФІТОПАТОГЕННИМ ГРИБОМ ACREMONIUM SP. 502 |
| title_full_unstemmed | СИНТЕЗ ЕТИЛЕНУ ФІТОПАТОГЕННИМ ГРИБОМ ACREMONIUM SP. 502 |
| title_short | СИНТЕЗ ЕТИЛЕНУ ФІТОПАТОГЕННИМ ГРИБОМ ACREMONIUM SP. 502 |
| title_sort | синтез етилену фітопатогенним грибом acremonium sp. 502 |
| topic | етилен фітопатоген Acremonium |
| topic_facet | ethylene phytopathogen Acremonium етилен фітопатоген Acremonium |
| url | https://smic.in.ua/index.php/journal/article/view/130 |
| work_keys_str_mv | AT cehmístergv synthesisofethylenewithacremoniumsp502phytopathogenicfungi AT cehmístergv sintezetilenufítopatogennimgribomacremoniumsp502 |