РОЗВИТОК TRICHODERMA HARZIANUM 128 НА РІЗНИХ ЕТАПАХ КОМПОСТУВАННЯ КУРЯЧОГО ПОСЛІДУ
The optimal periods of Trichoderma harzianum 128 introduction into an organic substrate within the model experiments on chicken dung composting were determined. Substrate inoculation with T. harzianum 128 (titer 128 thousand CFU/g of dry substrate) at the 2nd month of composting had resulted in the...
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| Sprache: | Englisch Ukrainisch |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2015
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Agriciltural microbiology| _version_ | 1871465427138248704 |
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| author | Деркач, С. М. Волкогон, В. В. Наконечна, Л. Т. Луценко, Н. В. Штанько, Н. П. |
| author_facet | Деркач, С. М. Волкогон, В. В. Наконечна, Л. Т. Луценко, Н. В. Штанько, Н. П. |
| author_institution_txt_mv | [
{
"author": "С. М. Деркач",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "В. В. Волкогон",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "Л. Т. Наконечна",
"institution": "Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України"
},
{
"author": "Н. В. Луценко",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "Н. П. Штанько",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Деркач, С. М. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:42Z |
| description | The optimal periods of Trichoderma harzianum 128 introduction into an organic substrate within the model experiments on chicken dung composting were determined. Substrate inoculation with T. harzianum 128 (titer 128 thousand CFU/g of dry substrate) at the 2nd month of composting had resulted in the rapid growth of the number of studied microorganism that reaches by the 7th month of composting the 9744 thousand CFU/g of dry compost. Proposed technique enables the receipt of compost with high number of the introduced microorganism. |
| doi_str_mv | 10.35868/1997-3004.21.3-6 |
| first_indexed | 2025-07-17T12:24:02Z |
| format | Article |
| fulltext |
DEVELOPMENT OF TRICHODERMA HARZIANUM 128
AT VARIOUS COMPOSTING STAGES OF CHICKEN DUNG
S. M. Derkach, V. V. Volkohon, L. T. Nakonechna, N. V. Lutsenko, N. P. Shtanko
Over the last decade poultry-farming
gained rapid development. This is according to
the State Statistics Service of Ukraine. Thus,
poultry population increased from 123.3
million in 1998 to 230.8 million in 2014. Such
active development of poultry-farming has led
to a significant accumulation of waste,
including manure – at the level of 1.5 mln tons
per year [1]. At the same time, poultry manure
is a valuable raw material for the production of
organic fertilizers [2]. Today there are several
technologies of manure processing that differ in
the nature of organic matter fermentation,
additional components use, duration of
processes, quality of output products. The
quality and safety of the final product in most
cases is determined by the dominant
microbiota, and, unfortunately, no existing
technology virtually takes into account the
features of microbiological processes that
occur during the period of manure composting
[3-5]. Along with traditional compost
production, promising is considered obtaining
bio-fertilizers with the participation of
agronomically useful microorganisms
introduced to compost mixtures [6-8].
Promising for organic matter composting can
be micromycetes of Trichoderma genus. Some
representatives of this taxonomic group have
valuable agronomic properties; in particular,
they are powerful biodestructors of organic
matter, possess antagonistic properties to a
number of pathogens of crops and produce
phytohormones [9; 10].
The objective of our research was to
determine the optimal introduction period of
Trichoderma harzianum 128 to organic
substrate.
Materials and methods. The research was
conducted during the 2013-2014 at the
laboratory of soil microbiology of the Institute
of Agricultural Microbiology and
Agroindustrial Manufacture of NAAS in the
conditions of model experiments. As chicken
manure has a narrow carbon to nitrogen ratio of
9.6:1, the composting involved preset
determination of optimum C:N ratio of 20:1 in
the substrate. Its optimization was performed
by mixing manure with peat and straw, as
carbon source, in the estimated quantities.
Plastic containers were used in the experiment,
to which 5 kg of chicken manure with humidity
70% were placed. 0.7 kg of chopped straw and
1.9 kg of peat were added to the manure by
mixing. The repetition of the experiment is
quadruple. The culture of saprophytic fungus T.
harzianum 128, selected by the authors as
active cellulose-digesting strain, was
introduced at various stages of substrate
composting according to the scheme of the
experiment:
1. Compost mixture without introduction
of selected microorganisms (control);
2. Introduction of T. harzianum 128 to the
compost mixture in the 1st month of
composting;
3. Introduction of T. harzianum 128 to the
compost mixture in the 2nd month of
composting;
4. Introduction of T. harzianum 128 to the
compost mixture in the 3rd month of
composting;
5. Introduction of T. harzianum 128 to the
compost mixture in the 4th month of
composting.
The suspension of T. harzianum 128 was
received by micromycete growing in test tubes
on slanted wort agar-agar, followed by rinsing
with water. The resulting suspension had a cell
concentration of 6.4 x 106 CFU/ml (in
conversion that amounted to 128 thousand
CFU/g of dry substrate). The suspension was
made in the amount of 2% of dry weight of
composted mixture.
The account of the number of T.
harzianum 128 was performed by agar plate
method [11].
Thus, the introduction of T. harzianum 128
is appropriate in the second month of manure
mixture composting with peat and straw; under
these conditions, the maximum number of
introduced microorganisms was at the level of
9744 thousand CFU/g of dry substrate in the
seventh month of composting; in the eighth
month of composting period the number of
microorganisms remained high enough
(8000-9000 thousand CFU/g of dry substrate),
this time coincides with the completion of the
process of organic matter composting.
Enriching the composts on the basis of
poultry manure with agronomically useful
microorganisms, which include the
representatives of Trichoderma harzianum, is a
promising area of biotechnology that can
provide both solving the problem of poultry
waste disposal, and a number of issues related
to the optimization of crops supply.
|
| id | oai:ojs2.smic.in.ua:article-138 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:01:51Z |
| publishDate | 2015 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/4c/188da1a02e63cbb13194c059f0772f4c.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1382026-07-22T10:10:42Z DEVELOPMENT OF TRICHODERMA HARZIANUM 128 AT VARIOUS COMPOSTING STAGES OF CHICKEN DUNG РОЗВИТОК TRICHODERMA HARZIANUM 128 НА РІЗНИХ ЕТАПАХ КОМПОСТУВАННЯ КУРЯЧОГО ПОСЛІДУ Деркач, С. М. Волкогон, В. В. Наконечна, Л. Т. Луценко, Н. В. Штанько, Н. П. Trichoderma harzianum, succession of microbial communities, agronomically valuable microorganisms, organic substrate, composts Trichoderma harzianum, сукцесії мікробних угруповань, агрономічно цінні мікроорганізми, органічний субстрат, компости The optimal periods of Trichoderma harzianum 128 introduction into an organic substrate within the model experiments on chicken dung composting were determined. Substrate inoculation with T. harzianum 128 (titer 128 thousand CFU/g of dry substrate) at the 2nd month of composting had resulted in the rapid growth of the number of studied microorganism that reaches by the 7th month of composting the 9744 thousand CFU/g of dry compost. Proposed technique enables the receipt of compost with high number of the introduced microorganism. В умовах модельного досліду з компостування курячого посліду визначали оптимальні періоди інтродукції Trichoderma harzianum 128 до органічного субстрату. Інокулювання субстрату T. harzianum 128 з титром 128 тис. КУО/г сухого субстрату на 2-й місяць компостування сприяє стрімкому зростанню чисельності досліджуваного мікроорганізму, що сягає на 7-й місяць компостування 9744 тис. КУО/г сухого компосту. Це дає можливість отримати компост з високим вмістом інтродукованого мікроорганізму. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2015-08-06 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/138 10.35868/1997-3004.21.3-6 Agricultural microbiology; Vol. 21 (2015): Agriciltural microbiology; 3-6 Сільськогосподарська мікробіологія; Том 21 (2015): Сільськогосподарська мікробіологія; 3-6 1997-3004 10.35868/1997-3004.21 en uk https://smic.in.ua/index.php/journal/article/view/138/162 https://smic.in.ua/index.php/journal/article/view/138/163 Авторське право (c) 2015 S. M. Derkach, V. V. Volkohon, L. T. Nakonechna, N. V. Lutsenko, N. P. Shtanko https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Trichoderma harzianum сукцесії мікробних угруповань агрономічно цінні мікроорганізми органічний субстрат компости Деркач, С. М. Волкогон, В. В. Наконечна, Л. Т. Луценко, Н. В. Штанько, Н. П. РОЗВИТОК TRICHODERMA HARZIANUM 128 НА РІЗНИХ ЕТАПАХ КОМПОСТУВАННЯ КУРЯЧОГО ПОСЛІДУ |
| title | РОЗВИТОК TRICHODERMA HARZIANUM 128 НА РІЗНИХ ЕТАПАХ КОМПОСТУВАННЯ КУРЯЧОГО ПОСЛІДУ |
| title_alt | DEVELOPMENT OF TRICHODERMA HARZIANUM 128 AT VARIOUS COMPOSTING STAGES OF CHICKEN DUNG |
| title_full | РОЗВИТОК TRICHODERMA HARZIANUM 128 НА РІЗНИХ ЕТАПАХ КОМПОСТУВАННЯ КУРЯЧОГО ПОСЛІДУ |
| title_fullStr | РОЗВИТОК TRICHODERMA HARZIANUM 128 НА РІЗНИХ ЕТАПАХ КОМПОСТУВАННЯ КУРЯЧОГО ПОСЛІДУ |
| title_full_unstemmed | РОЗВИТОК TRICHODERMA HARZIANUM 128 НА РІЗНИХ ЕТАПАХ КОМПОСТУВАННЯ КУРЯЧОГО ПОСЛІДУ |
| title_short | РОЗВИТОК TRICHODERMA HARZIANUM 128 НА РІЗНИХ ЕТАПАХ КОМПОСТУВАННЯ КУРЯЧОГО ПОСЛІДУ |
| title_sort | розвиток trichoderma harzianum 128 на різних етапах компостування курячого посліду |
| topic | Trichoderma harzianum сукцесії мікробних угруповань агрономічно цінні мікроорганізми органічний субстрат компости |
| topic_facet | Trichoderma harzianum succession of microbial communities agronomically valuable microorganisms organic substrate composts Trichoderma harzianum сукцесії мікробних угруповань агрономічно цінні мікроорганізми органічний субстрат компости |
| url | https://smic.in.ua/index.php/journal/article/view/138 |
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