ОЦІНКА ПАТОГЕННОСТІ ҐРУНТОВИХ ГРИБІВ РОДУ CLADOSPORIUM
Verification of soil molds from Cladosporium genus was performed in acute experiments with white mice. LD50 of the studied strains was determined followed by the pathological and bacteriological examinations of the inner organs of laboratory animals. It was revealed that studied Cladosporium strains...
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| Дата: | 2015 |
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| Автори: | , , , |
| Формат: | Стаття |
| Мова: | Англійська Українська |
| Опубліковано: |
Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2015
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| Онлайн доступ: | https://smic.in.ua/index.php/journal/article/view/139 |
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| Назва журналу: | Agriciltural microbiology |
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Репозитарії
Agriciltural microbiology| _version_ | 1871465427208503296 |
|---|---|
| author | Кравченко, Н. О. Копилова, О. Б. Головач, О. В. Дмитрук, О. М. |
| author_facet | Кравченко, Н. О. Копилова, О. Б. Головач, О. В. Дмитрук, О. М. |
| author_institution_txt_mv | [
{
"author": "Н. О. Кравченко",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "О. Б. Копилова",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "О. В. Головач",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "О. М. Дмитрук",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Кравченко, Н. О. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:42Z |
| description | Verification of soil molds from Cladosporium genus was performed in acute experiments with white mice. LD50 of the studied strains was determined followed by the pathological and bacteriological examinations of the inner organs of laboratory animals. It was revealed that studied Cladosporium strains belong to the group of avirulent microorganisms not capable of invasion of the inner organs of the studied warm-blooded animals and thus can be considered as non-pathogenic. |
| doi_str_mv | 10.35868/1997-3004.21.7-11 |
| first_indexed | 2025-07-17T12:24:03Z |
| format | Article |
| fulltext |
EVALUATION OF CLADOSPORIUM SOIL MOLDS PATHOGENICITY
N. O. Kravchenko, O. B. Kopylova, O. V. Golovach, O. M. Dmytruk
Soil fungi play an important role in the
nature. According to modern concepts fungi
kingdom is one of the most important
components of ecosystems and includes about
1.5 million species [1]. Typically, the fungi
biomass in arable or meadow soils is as big as
bacteria biomass [2].
Fungi are involved in the destruction of
organic matter at soil formation as well as in the
processes of biogeochemical transformations
of elements (N, P, K, S), and so on. In addition,
fungi closely interact with plants, forming
mycorrhiza and synthesizing biologically
active substances.
One of the prominent producers of
biologically active substances are considered to
be the representatives of Cladosporium Link
genus, first described in 1816, numbering 169
species and being the most common among
imperfect fungi [3].
Note, that the specified genus is
characterized by high diversity and includes
endophytic, saprotrophic and phytopathogenic
fungi. In addition, the representatives of
Cladosporium genus can be agents of animal
diseases [4; 5].
The representatives of Cladosporium
genus are isolated out from virtually all known
substrates – soil, fallen leaves, stems of
herbaceous and woody plants, and can be
concomitant with opportunistic infections
caused by other pathogens [6-8]. In addition,
Cladosporium herbarum is known as
hyperallergenic agent [9].
The search for new strains of fungi of
Cladosporium genus, capable of producing
biologically active substances and positive
impact on the growth and development of
plants and their use as potential agents of
biological products is a promising area of
agricultural microbiology. Given the fact that
among Cladosporium genus not only
pathogens of plants but also animals occur,
promising strains should be safe for humans
and warm-blooded animals.
The objective of our work was to study the
properties of soil pathogenic fungi of
Cladosporium genus that are characterized by
the ability to produce phytohormonal
substances and can be used as the basis for
biological products with growth-stimulating
activity.
Materials and methods. The strains of
fungi of Cladosporium genus S.
cladosporioides (Fresen.) G. A. de Vries.
species. C. cladosporioides 525 strain was
isolated from soil rhizospheric soil of spring
wheat, C. cladosporioides 495 – from the roots
of white lupine of Lybid variety.the strains
were grown on wort agar-agar 3-4° by Baling,
pH 6.4-7.0 at the temperature of 25-27°C.
The suspensions of spore-mycelium fungi
mixtures were prepared in sterile isotonic
sodium chloride solution following two-times
washing from metabolites and deposition of
cells by centrifugation for 20 min. At 2000
rev./min. Working concentration of fungi cells
in suspensions were set using Goriaiev
chamber.
Pathogenic properties of strains were
determined in acute experiments on the model
of white outbred mice. Mature white mice
weighing 18-20 g were administered with
spore-mycelium fungi mixtures orally through
a tube and by intraperitoneal injection. The
doses for oral administration contained
1.0x106; 1.0x107; 1.0x108 cells of soil fungi, for
intraperitoneal injection – 1.0x107 and 1.0x108
cells per animal [10]. Laboratory animals had
been were previously adapted to the conditions
within 14 days. Care and observations had been
conducted daily for 20 consecutive days after
the administration of test material [10; 11].
At the end of the observation period on
behavioural and physiological responses of
laboratory animals their compulsory slaughter
and mortem were performed, followed by
microscopic study of smears-prints of internals
and seeding of tissue samples (liver, kidney,
spleen) for elective nutrient medium.
Housing, feeding, care and all
manipulations with laboratory animals were
carried out under the European Convention
“For the protection of vertebrate animals used
for experimental and other scientific purposes”
(Strasbourg, 1986) [12] and “General ethical
principles of experiments on animals”
approved at the first National Congress on
Bioethics (Kyiv, 2001) [13]. Experiments were
conducted in compliance with the principles of
humanity set out in the European Community
Directive [14].
The virulence of soil fungi strains was
established by the level of dose of viable
microbial cells, which causes death in 50% of
infected animals (LD50). The criterion for
avirulence of microorganisms was the absence
of death of mice within 20 days and
characteristic for infectious diseases changes in
the internal organs of animals by the results of
post-mortem examination.
The infectivity (invasiveness) of fungi
strains was determined by seeding of tissue
samples (liver, kidney, spleen) of compulsorily
slaughtered animals on wort agar-agar. The
lack of typical for investigated soil fungi strains
growth pointed to their non-infectivity.
So, the obtained results indicate the
avirulence of soil fungi C. cladosporioides 495
and C. cladosporioides 525 as the average
lethal dose for oral and intraperitoneal
administration of their suspensions is LD50 per os
>1.0x108 cells/animal, LD50 i/p >1.0x108
cells/animal. The mortem of compulsorily
slaughtered white mice of all experimental and
control groups showed no visible signs of
pathological changes in the internal organs of
animals after the administration of soil fungi C.
cladosporioides 495 and C. cladosporioides
525 (table 2).
By the results of microbiological tests of
samples of the internal organs of animals of
both experimental and control groups the
growth of retro-cultures of studied fungi was
not established. Thus, we can conclude that soil
fungi C. cladosporioides 495 and C.
cladosporioides 525 in applied concentrations
do not penetrate the internal organs of animals,
do not breed in them and do not cause changes
typical for infection process. The avirulence of
above mentioned fungi strains of
Cladosporium genus for warm-blooded
animals is confirmed by the data of medium
lethal doses by both oral and intraperitoneal
administration.
So, according to the results, soil fungi C.
cladosporioides 495 and C. cladosporioides
525 belong to the group of avirulent
microorganisms incapable of invasion into
internals of investigated warm-blooded animals
and can be used as the basis for
growth-stimulating biological preparations.
|
| id | oai:ojs2.smic.in.ua:article-139 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:01:51Z |
| publishDate | 2015 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/b0/56492fb276225cd57e09feb64b49f5b0.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1392026-07-22T10:10:42Z EVALUATION OF CLADOSPORIUM SOIL MOLDS PATHOGENICITY ОЦІНКА ПАТОГЕННОСТІ ҐРУНТОВИХ ГРИБІВ РОДУ CLADOSPORIUM Кравченко, Н. О. Копилова, О. Б. Головач, О. В. Дмитрук, О. М. Cladosporium cladosporioides, virulence, physiological condition of mice, pathogenicity, infectivity (invasiveness) Cladosporium cladosporioides, вірулентність, фізіологічний стан мишей, патогенність, інфективність (інвазивність) Verification of soil molds from Cladosporium genus was performed in acute experiments with white mice. LD50 of the studied strains was determined followed by the pathological and bacteriological examinations of the inner organs of laboratory animals. It was revealed that studied Cladosporium strains belong to the group of avirulent microorganisms not capable of invasion of the inner organs of the studied warm-blooded animals and thus can be considered as non-pathogenic. Проведено перевірку патогенних властивостей ґрунтових грибів роду Cladosporium Link у гострих дослідах на моделі білих мишей. Визначено LD50 штамів, проведено патологоанатомічні та бактеріологічні дослідження внутрішніх органів лабораторних тварин. Досліджені штами належать до групи авірулентних мікроорганізмів, не здатних до інвазії у внутрішні органи досліджуваних теплокровних тварин і можуть вважатися непатогенними. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2015-08-06 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/139 10.35868/1997-3004.21.7-11 Agricultural microbiology; Vol. 21 (2015): Agriciltural microbiology; 7-11 Сільськогосподарська мікробіологія; Том 21 (2015): Сільськогосподарська мікробіологія; 7-11 1997-3004 10.35868/1997-3004.21 en uk https://smic.in.ua/index.php/journal/article/view/139/164 https://smic.in.ua/index.php/journal/article/view/139/165 Авторське право (c) 2015 N. O. Kravchenko, O. B. Kopylova, O. V. Golovach, O. M. Dmytruk https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Cladosporium cladosporioides вірулентність фізіологічний стан мишей патогенність інфективність (інвазивність) Кравченко, Н. О. Копилова, О. Б. Головач, О. В. Дмитрук, О. М. ОЦІНКА ПАТОГЕННОСТІ ҐРУНТОВИХ ГРИБІВ РОДУ CLADOSPORIUM |
| title | ОЦІНКА ПАТОГЕННОСТІ ҐРУНТОВИХ ГРИБІВ РОДУ CLADOSPORIUM |
| title_alt | EVALUATION OF CLADOSPORIUM SOIL MOLDS PATHOGENICITY |
| title_full | ОЦІНКА ПАТОГЕННОСТІ ҐРУНТОВИХ ГРИБІВ РОДУ CLADOSPORIUM |
| title_fullStr | ОЦІНКА ПАТОГЕННОСТІ ҐРУНТОВИХ ГРИБІВ РОДУ CLADOSPORIUM |
| title_full_unstemmed | ОЦІНКА ПАТОГЕННОСТІ ҐРУНТОВИХ ГРИБІВ РОДУ CLADOSPORIUM |
| title_short | ОЦІНКА ПАТОГЕННОСТІ ҐРУНТОВИХ ГРИБІВ РОДУ CLADOSPORIUM |
| title_sort | оцінка патогенності ґрунтових грибів роду cladosporium |
| topic | Cladosporium cladosporioides вірулентність фізіологічний стан мишей патогенність інфективність (інвазивність) |
| topic_facet | Cladosporium cladosporioides virulence physiological condition of mice pathogenicity infectivity (invasiveness) Cladosporium cladosporioides вірулентність фізіологічний стан мишей патогенність інфективність (інвазивність) |
| url | https://smic.in.ua/index.php/journal/article/view/139 |
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