ПРОДУКЦІЙНИЙ ПРОЦЕС ЯЧМЕНЮ ЯРОГО ЗА ІНОКУЛЯЦІЇ МІКРОГУМІНОМ З ОПТИМІЗОВАНИМ ВМІСТОМ ФІТОГОРМОНІВ
The paper presents the experimental results of spring barley inoculation with microbial preparation Mikrohumin with optimized phytohormones content on plant development during the early stages of plants organogenesis obtained in the greenhouse experiment on sod-podzolic silty-sandy soils. It was sho...
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| Date: | 2015 |
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| Main Author: | |
| Format: | Article |
| Language: | English Ukrainian |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2015
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| Online Access: | https://smic.in.ua/index.php/journal/article/view/142 |
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| Journal Title: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465587211763712 |
|---|---|
| author | Дімова, С. Б. |
| author_facet | Дімова, С. Б. |
| author_institution_txt_mv | [
{
"author": "С. Б. Дімова",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Дімова, С. Б. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:42Z |
| description | The paper presents the experimental results of spring barley inoculation with microbial preparation Mikrohumin with optimized phytohormones content on plant development during the early stages of plants organogenesis obtained in the greenhouse experiment on sod-podzolic silty-sandy soils. It was shown that under the seeds inoculation with the microbial preparation plant hormones are served as the additional factor of positive influence on the plants. |
| doi_str_mv | 10.35868/1997-3004.21.25-30 |
| first_indexed | 2025-07-17T12:24:04Z |
| format | Article |
| fulltext |
THE PRODUCTION PROCESS OF SPRING BARLEY
UNDER THE INOCULATION WITH MIKROHUMIN
WITH OPTIMIZED PLANT HORMONES CONTENT
S. B. Dimova
The success of pre-seed inoculation with
biological products, bio-agents of which are
microorganisms that are able to form
associations with plants, depends on the initial
conditions of interaction of introduced bacteria
with the roots of juvenile plants. This process
can be affected by several factors. Today there
are enough experimental data which prove that
growth substances – phytohormones (auxins
and cytokinins first of all) occupy a special
place in the regulation of the relationships of
associative nitrogen fixers and cereal plants [1; 2].
In this regard, obvious is the need to
optimize the content of these substances in
microbial preparations that will help receive
fertilizers with high and stable performance.
Pre-seed inoculation with biological products
containing optimum amount of physiologically
active substances will provide an opportunity to
intensify the formation of effective associations
of microorganisms with plants.
In previous studies we set optimal
parameters of phytohormones content to
enhance the activity of individual
growth-stimulating activity of particular
microbial drugs (including Mikrohumin)
through biotesting. The objective of this study
was to test the efficiency of biological
preparation Mikrohumin with optimized
content of physiologically active substances.
Materials and methods. During the
vegetative experiment the effectiveness of
pre-seed inoculation of spring barley of Hosia
variety with the suspension containing
nitrogen-fixing associative bacteria
Azospirillum brasilense 410 (Mikrohumin
bio-agents) was studied as well as with the same
suspension combined with the solution of
phytohormones (model of Mikrohumin
microbial drug with optimized content of
phytohormones). The content of indoleacetic
acid (IAA) and cytokinin (zeatin and zeatin
riboside) in investigated preparations was
determined by solid phase ELISA method [3; 4].
Background phytohormones content in
bacterial suspensions composed 0.75 g/cm3 of
IAA and 0.22 g/cm3 of cytokinins. Optimized
against phytohormonal load preparation
contained 2.63 g/cm3 of IAA and 0.56 g/cm3 of
cytokinins.
The experiment was carried out in a
vegetative house in vessels with the capacity of
1 dm3 on sod-podzolic silty-sabulous soil (rNsol.
7.2, humus - 1.02%; P2O5 - 330 mg/kg; K2O -
148 mg/kg). Prianishnikov mixture with half a
dose of nitrogen was administered to the soil
before sowing, given the inoculation with
associative nitrogen-fixing bacteria A.
brasilense 410, able to improve barley plant
nitrogen nutrition.
Above-ground mass of plants was
determined by gravimetrically after drying to
constant indicators. The mass of roots was
determined by washing the from monoliths,
followed by drying to constant weight,
chlorophyll content in leaves –
spectrophotometrically [5], protein content – by
Lowry method [6]. Potential nitrogen fixation
activity was determined in rhizospheric soil
with the addition of glucose solution [7; 8], and
on the washed roots of spring barley - in
semisolid Dobereiner culture medium [9]. At
this the acetylene reduction was determined at
chromatograph “Chrom-4” with a flame
ionization detector on the column with Porapak
Q. The potential denitrification activity in
rhizospheric soil and washed roots was studied
by acetylene method while adding glucose
solution and potassium nitrate [10]. The
reduction of nitrous oxide was determined at
chromatograph “Tsvet-60 M” with thermal
conductivity detector on the column with
Porapak Q sorbent. Statistical analysis of the
results was performed by Dospiekhov [11].
Thus, it was found that the combination of
pre-seed inoculation with the optimum amount
of phytohormones positively affects the
development of spring barley plants in early
stages of organogenesis. At this,
phytohormones are an additional factor of
positive influence on plants. The use of
microbial preparation with optimized content of
phytohormones (2.63 g/cm3 of IAA and 0.56
g/cm3 of cytokinins) affects the biological
activity in barley plants rhizosphere – namely,
positively impacts the process of biological
nitrogen transformation in the root zone. It can
provide additional nitrogen nutrition, promotes
root system and aerial parts development, the
formation of photosynthetic apparatus of plants
and protein synthesis.
The method for producing microbial
preparation was offered whereby the essential
phytohormones are added to the microbial
component of the preparation in the amounts
previously set by biotesting.
|
| id | oai:ojs2.smic.in.ua:article-142 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:04:24Z |
| publishDate | 2015 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/57/8e10e2ef7337d015230c481edb0ef857.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1422026-07-22T10:10:42Z THE PRODUCTION PROCESS OF SPRING BARLEY UNDER THE INOCULATION WITH MIKROHUMIN WITH OPTIMIZED PLANT HORMONES CONTENT ПРОДУКЦІЙНИЙ ПРОЦЕС ЯЧМЕНЮ ЯРОГО ЗА ІНОКУЛЯЦІЇ МІКРОГУМІНОМ З ОПТИМІЗОВАНИМ ВМІСТОМ ФІТОГОРМОНІВ Дімова, С. Б. plant hormones, β-indolyl-3- acetic acid, cytokinins, seeds bakteryzation фітогормони, β-індоліл-3-оцтова кислота, цитокініни, бактеризація насіння The paper presents the experimental results of spring barley inoculation with microbial preparation Mikrohumin with optimized phytohormones content on plant development during the early stages of plants organogenesis obtained in the greenhouse experiment on sod-podzolic silty-sandy soils. It was shown that under the seeds inoculation with the microbial preparation plant hormones are served as the additional factor of positive influence on the plants. У вегетаційному досліді на дерново-підзолистому пилувато-супіщаному ґрунті досліджено вплив мікробного препарату Мікрогуміну з оптимізованим вмістом фітогормонів на розвиток рослин ячменю ярого на початкових етапах органогенезу. Показано, що при інокуляції насіння мікробним препаратом фітогормони є додатковим чинником позитивного впливу на рослини. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2015-08-06 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/142 10.35868/1997-3004.21.25-30 Agricultural microbiology; Vol. 21 (2015): Agriciltural microbiology; 25-30 Сільськогосподарська мікробіологія; Том 21 (2015): Сільськогосподарська мікробіологія; 25-30 1997-3004 10.35868/1997-3004.21 en uk https://smic.in.ua/index.php/journal/article/view/142/170 https://smic.in.ua/index.php/journal/article/view/142/171 Авторське право (c) 2015 S. B. Dimova https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | фітогормони β-індоліл-3-оцтова кислота цитокініни бактеризація насіння Дімова, С. Б. ПРОДУКЦІЙНИЙ ПРОЦЕС ЯЧМЕНЮ ЯРОГО ЗА ІНОКУЛЯЦІЇ МІКРОГУМІНОМ З ОПТИМІЗОВАНИМ ВМІСТОМ ФІТОГОРМОНІВ |
| title | ПРОДУКЦІЙНИЙ ПРОЦЕС ЯЧМЕНЮ ЯРОГО ЗА ІНОКУЛЯЦІЇ МІКРОГУМІНОМ З ОПТИМІЗОВАНИМ ВМІСТОМ ФІТОГОРМОНІВ |
| title_alt | THE PRODUCTION PROCESS OF SPRING BARLEY UNDER THE INOCULATION WITH MIKROHUMIN WITH OPTIMIZED PLANT HORMONES CONTENT |
| title_full | ПРОДУКЦІЙНИЙ ПРОЦЕС ЯЧМЕНЮ ЯРОГО ЗА ІНОКУЛЯЦІЇ МІКРОГУМІНОМ З ОПТИМІЗОВАНИМ ВМІСТОМ ФІТОГОРМОНІВ |
| title_fullStr | ПРОДУКЦІЙНИЙ ПРОЦЕС ЯЧМЕНЮ ЯРОГО ЗА ІНОКУЛЯЦІЇ МІКРОГУМІНОМ З ОПТИМІЗОВАНИМ ВМІСТОМ ФІТОГОРМОНІВ |
| title_full_unstemmed | ПРОДУКЦІЙНИЙ ПРОЦЕС ЯЧМЕНЮ ЯРОГО ЗА ІНОКУЛЯЦІЇ МІКРОГУМІНОМ З ОПТИМІЗОВАНИМ ВМІСТОМ ФІТОГОРМОНІВ |
| title_short | ПРОДУКЦІЙНИЙ ПРОЦЕС ЯЧМЕНЮ ЯРОГО ЗА ІНОКУЛЯЦІЇ МІКРОГУМІНОМ З ОПТИМІЗОВАНИМ ВМІСТОМ ФІТОГОРМОНІВ |
| title_sort | продукційний процес ячменю ярого за інокуляції мікрогуміном з оптимізованим вмістом фітогормонів |
| topic | фітогормони β-індоліл-3-оцтова кислота цитокініни бактеризація насіння |
| topic_facet | plant hormones β-indolyl-3- acetic acid cytokinins seeds bakteryzation фітогормони β-індоліл-3-оцтова кислота цитокініни бактеризація насіння |
| url | https://smic.in.ua/index.php/journal/article/view/142 |
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