ВПЛИВ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE НА ВЕРТИКАЛЬНУ МІГРАЦІЮ СПОЛУК БІОГЕННИХ ЕЛЕМЕНТІВ ЗА ВИРОЩУВАННЯ СОЇ
The paper presents the research results of pre-sowing seeds bacterization with nitrogen fixing bacteria Bradyrhizobium japonicum and Azospirillum brasilense influence on the loss of moisture, water soluble humus and nutrients. It was shown that seeds bacterization reduces the leaching intensity of n...
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| Sprache: | Englisch Ukrainisch |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2015
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Agriciltural microbiology| _version_ | 1871465589392801792 |
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| author | Козар, С. Ф. Пищур, І. М. Нестеренко, В. М. |
| author_facet | Козар, С. Ф. Пищур, І. М. Нестеренко, В. М. |
| author_institution_txt_mv | [
{
"author": "С. Ф. Козар",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "І. М. Пищур",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "В. М. Нестеренко",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Козар, С. Ф. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:43Z |
| description | The paper presents the research results of pre-sowing seeds bacterization with nitrogen fixing bacteria Bradyrhizobium japonicum and Azospirillum brasilense influence on the loss of moisture, water soluble humus and nutrients. It was shown that seeds bacterization reduces the leaching intensity of nitrates, phosphorus, potassium, calcium and magnesium. Moreover, the least losses of nutrients were observed in a variants with joint application of both studied nitrogen fixing microorganisms. It was noted that seeds bacterization with B. japonicum and A. brasilense had promoted increase of chlorophyll content in the leaves of soybean plants. The highest yield was observed in the variant with the joint use of rhizobia and azospirillum. |
| doi_str_mv | 10.35868/1997-3004.21.39-43 |
| first_indexed | 2025-07-17T12:24:05Z |
| format | Article |
| fulltext |
THE INFLUENCE OF BRADYRHIZOBIUM JAPONICUM
AND AZOSPIRILLUM BRASILENSE ON THE VERTICAL MIGRATION
OF NUTRIENTS AT SOYBEAN CULTIVATION
S. F. Kozar, I. M. Pyschur, V. M. Nesterenko
Nitrogen-fixing bacteria developing in
plants root area are able to improve their
nutrition and boost the growth and development
of crops. Particularly effective are nodule
bacteria that form symbiotic systems with
various legumes, including soy [1]. The
maximum realization of rhizobia-plant systems
potential can be achieved by the incorporation
of all abiotic and biotic environmental factors
effect, one of which is the impact on symbiotic
diazotrophs of associative or nonsymbiotic
nitrogen-fixing bacteria.
There are some author’s research results
that indicate the possibility of root growth and
nodulation stimulation in soybean plants by the
use of azospirils in conjunction with the
inoculation with different strains of rhizobia
[2-4]. It was shown that microorganisms
introduced in agrocenosis can help increase the
length and dry mass of roots and growth of root
hairs. The enhanced root development after the
application of mixed culture of microorganisms,
in our view, should provide a reduction of
nutrients losses with vertical water flow by the
soil profile observed in the conditions of
flushing regime.
The objective of our work was to study the
effect of soybean pre-seed bacterization with
diazotrophs Bradyrhizobium japonicum and
Azospirillum brasilense on the intensity of
vertical migration of nutrients beyond the root
layer of soil.
Materials and methods. The effect of
soybean pre-seed bacterization on vertical
migration of compounds of nutrients was
studied in the terms of lysimeter installation of
the Institute of Agricultural Microbiology and
Agroindustrial Manufacture of NAAS,
containing 48 sections-lysimeters placed in two
parallel rows of 24 lysimeters each.
Vessels-receivers for infiltration collection are
placed beneath. By construction the lysimeters
are made of concrete, bulk type. Lysimeter cells
are filled with soil sequentially, beginning with
the parent rock, taking into account the power of
genetic horizon. Lysimeter cell cultivated area
is 3.8 m2, soil layer - 155 cm, its weight - 10.5
tons. Soil in lysimeters - sod-podzolic sabulous
with such agrochemical characteristics of arable
layer (0-23 cm): humus content by Tiurin -
1.1%; salt extraction pH - 5.5; hydrolytic acidity
(by Kappen) - 2.5 mg eq. Per 100 g; P2O5
content (by Kirsanov) - 170.0 mg; K2O (by
Maslova) - 62.0 mg per 1 kg of soil. Experiment
with soy of Lehenda variety was performed in
2014. The repetition in the experiment is
quadruple. The scheme of the experiment is
shown in the tables.
Nitrate content was determined by
phenoldisulfonic method, ammonium content -
with Nesler reagent, water-soluble P2O5 – by
Kirsanov, K2O – by flame photometric method,
CaO and MgO – by complexometric method,
water soluble humus - by Tiurin [5].
Harvest collection and records were
performed by direct method. Statistical analysis
of the results was performed by Dospiekhov [6].
The content of chlorophylls a and b was
determined spectrophotometric method [7].
Bacterial strains B. japonicum M-8 [8] and
A. brasilense 410 were used in the experiment
[9]. The microorganisms were obtained from
the National Collection of useful soil
microorganisms of the Institute of Agricultural
Microbiology and Agroindustrial Manufacture
of NAAS.
The studied microorganisms were grown in
the conditions of periodic cultivation on
microbiological rocking at 220 rev/min and
28°C. Seeding culture of A. brasilense was
grown in liquid culture medium of such
composition (g/dm3): maize extract - 30.0;
molasses - 30.0; (NH4) 2SO4 – 0.10; KH2PO4 –
0.25; K2HPO4*3H2O – 0.25; MgSO4*7H2O –
0.20; CaCO3 - 0.30. Seeding culture B.
japonicum was grown in liquid culture medium
of such composition (g/dm3): peas seed broth -
100; glucose - 10; sucrose - 5; (NH4) SO4 – 1.0;
KN2RO4 - 0.5; K2NRO4*3N2O - 0.5;
MgSO4*7H2O – 0.2; CaCO - 0.3. Co-culturing
of A. brasilense and B. japonicum bacteria was
performed using a mixed culture medium for
culturing rhizobia and azospiryls [10].
We express our sincere gratitude to the
authors for kindly furnished strains, and the
staff of Agricultural Chemistry and Soil
Fertility laboratory of the Institute of
Agricultural Microbiology and Agroindustrial
Manufacture of NAAS for assistance in the
study.
The records of soybean yields showed that
pre-plant inoculation of seeds with nodule
bacteria helped increase the culture
productivity. There was no significant effect of
A. brasilense 410 on productivity. The highest
increase in soybean harvest against control was
observed in the variant with common use of B.
japonicum M-8 and A. brasilense 410 (Table.
2). While combined application of rhizobia and
azospiryls helped increase the yields by 19.2%
compared with the use of pure B. japonicum
culture.
Thus, the use of B. japonicum and A.
brasilense in soybean growing technology there
is a significant decrease in the loss of moisture,
water soluble humus compounds and nutrients,
and increase in the content of chlorophyll a and
b in leaves of soybean plants. At combined use
of rhizobia and azospiryls the synergistic effect
as for the increase of yields of this legume is
observed.
|
| id | oai:ojs2.smic.in.ua:article-144 |
| institution | Agriciltural microbiology |
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| language | English Ukrainian |
| last_indexed | 2026-07-23T01:04:26Z |
| publishDate | 2015 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/8f/90d57038c0ca0d9f18c07075cdf5d18f.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1442026-07-22T10:10:43Z THE INFLUENCE OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE ON THE VERTICAL MIGRATION OF NUTRIENTS AT SOYBEAN CULTIVATION ВПЛИВ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE НА ВЕРТИКАЛЬНУ МІГРАЦІЮ СПОЛУК БІОГЕННИХ ЕЛЕМЕНТІВ ЗА ВИРОЩУВАННЯ СОЇ Козар, С. Ф. Пищур, І. М. Нестеренко, В. М. Bradyrhizobium japonicum, Azospirillum brasilense, soybean inoculation, lyzimetr, nutrients Bradyrhizobium japonicum, Azospirillum brasilense, соя, інокуляція, лізиметри, біогенні елементи The paper presents the research results of pre-sowing seeds bacterization with nitrogen fixing bacteria Bradyrhizobium japonicum and Azospirillum brasilense influence on the loss of moisture, water soluble humus and nutrients. It was shown that seeds bacterization reduces the leaching intensity of nitrates, phosphorus, potassium, calcium and magnesium. Moreover, the least losses of nutrients were observed in a variants with joint application of both studied nitrogen fixing microorganisms. It was noted that seeds bacterization with B. japonicum and A. brasilense had promoted increase of chlorophyll content in the leaves of soybean plants. The highest yield was observed in the variant with the joint use of rhizobia and azospirillum. Представлено результати досліджень щодо втрат вологи, водорозчинного гумусу та сполук біогенних елементів за впливу передпосівної інокуляції насіння сої діазотрофами Bradyrhizobium japonicum і Azospirillum brasilense. Виявлено, що бактеризація сприяє зменшенню інтенсивності вимивання нітратів, сполук фосфору, калію, кальцію і магнію. При цьому найменші втрати біогенних елементів спостерігали у варіанті з сумісним застосуванням азотфіксувальних мікроорганізмів. Відмічено, що за дії бактерій B. japonicum і A. brasilense збільшується вміст хлорофілів у листках рослин сої. Найвищу урожайність відмічено у варіанті з використанням змішаної культури бульбочкових бактерій і азоспірил. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2015-08-06 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/144 10.35868/1997-3004.21.39-43 Agricultural microbiology; Vol. 21 (2015): Agriciltural microbiology; 39-43 Сільськогосподарська мікробіологія; Том 21 (2015): Сільськогосподарська мікробіологія; 39-43 1997-3004 10.35868/1997-3004.21 en uk https://smic.in.ua/index.php/journal/article/view/144/174 https://smic.in.ua/index.php/journal/article/view/144/175 Авторське право (c) 2015 S. F. Kozar, I. M. Pyschur, V. M. Nesterenko https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Bradyrhizobium japonicum Azospirillum brasilense соя інокуляція лізиметри біогенні елементи Козар, С. Ф. Пищур, І. М. Нестеренко, В. М. ВПЛИВ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE НА ВЕРТИКАЛЬНУ МІГРАЦІЮ СПОЛУК БІОГЕННИХ ЕЛЕМЕНТІВ ЗА ВИРОЩУВАННЯ СОЇ |
| title | ВПЛИВ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE НА ВЕРТИКАЛЬНУ МІГРАЦІЮ СПОЛУК БІОГЕННИХ ЕЛЕМЕНТІВ ЗА ВИРОЩУВАННЯ СОЇ |
| title_alt | THE INFLUENCE OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE ON THE VERTICAL MIGRATION OF NUTRIENTS AT SOYBEAN CULTIVATION |
| title_full | ВПЛИВ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE НА ВЕРТИКАЛЬНУ МІГРАЦІЮ СПОЛУК БІОГЕННИХ ЕЛЕМЕНТІВ ЗА ВИРОЩУВАННЯ СОЇ |
| title_fullStr | ВПЛИВ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE НА ВЕРТИКАЛЬНУ МІГРАЦІЮ СПОЛУК БІОГЕННИХ ЕЛЕМЕНТІВ ЗА ВИРОЩУВАННЯ СОЇ |
| title_full_unstemmed | ВПЛИВ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE НА ВЕРТИКАЛЬНУ МІГРАЦІЮ СПОЛУК БІОГЕННИХ ЕЛЕМЕНТІВ ЗА ВИРОЩУВАННЯ СОЇ |
| title_short | ВПЛИВ BRADYRHIZOBIUM JAPONICUM І AZOSPIRILLUM BRASILENSE НА ВЕРТИКАЛЬНУ МІГРАЦІЮ СПОЛУК БІОГЕННИХ ЕЛЕМЕНТІВ ЗА ВИРОЩУВАННЯ СОЇ |
| title_sort | вплив bradyrhizobium japonicum і azospirillum brasilense на вертикальну міграцію сполук біогенних елементів за вирощування сої |
| topic | Bradyrhizobium japonicum Azospirillum brasilense соя інокуляція лізиметри біогенні елементи |
| topic_facet | Bradyrhizobium japonicum Azospirillum brasilense soybean inoculation lyzimetr nutrients Bradyrhizobium japonicum Azospirillum brasilense соя інокуляція лізиметри біогенні елементи |
| url | https://smic.in.ua/index.php/journal/article/view/144 |
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