ОСОБЛИВОСТІ ПРОЦЕСІВ АВТОТРОФНОЇ І ГЕТЕРОТРОФНОЇ НІТРИФІКАЦІЇ В КОРЕНЕВІЙ ЗОНІ РОСЛИН ЖИТА ОЗИМОГО ЗА ВПЛИВУ МІНЕРАЛЬНИХ ДОБРИВ ТА ДІАЗОБАКТЕРИНУ
The paper depicts the study of mineral nitrogen and pre-sowing seeds bacteryzation on the development of nitrogen fixing bacteria and the activity of autotrophic and heterotrophic nitrification. It was revealed that nitrification activity in the root zone of winter rye plants rises together with the...
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2015
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| author | Коротка, І. Г. Волкогон, В. В. |
| author_facet | Коротка, І. Г. Волкогон, В. В. |
| author_institution_txt_mv | [
{
"author": "І. Г. Коротка",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "В. В. Волкогон",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Коротка, І. Г. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:43Z |
| description | The paper depicts the study of mineral nitrogen and pre-sowing seeds bacteryzation on the development of nitrogen fixing bacteria and the activity of autotrophic and heterotrophic nitrification. It was revealed that nitrification activity in the root zone of winter rye plants rises together with the increase of mineral nitrogen doses. Heterotrophic nitrification plays a significant role in the formation of nitrate pool in the root zone, especially during the early stages of plants organogenesis. Application of the microbial preparation Diazobakteryn had enhanced the activity autotrophic and heterotrophic nitrification processes in the rhizosphere soil of plants in the spring, during the early stages of their development while during the next organogenesis phases the reduction of nitrification processes was observed. In plants-free soil the contribution of heterotrophic nitrification to biological transformation of nitrogen was negligible. |
| doi_str_mv | 10.35868/1997-3004.21.44-51 |
| first_indexed | 2025-07-17T12:24:05Z |
| format | Article |
| fulltext |
44
Сільськогосподарська мікробіологія. — 2015. — Вип. 21. — С. 44–51.
ISSN 1997-3004
UDC 579:631.461
THE FEATURES THE AUTOTROPHIC AND HETEROTROPHIC
NITRIFICATION IN THE ROOT ZONE OF WINTER RYE
PLANTS UNDER THE USE OF MINERAL FERTILIZERS
AND DIAZOBAKTERYN
I. G. Korotka, V. V. Volkogon
Institute of Agricultural Microbiology and Agroindustrial Manufacture, UAAS
97, Shevchenka str., Chernihiv, 14027, Ukraine; e-mail: chychvaga_i@i.ua
The paper depicts the study of mineral nitrogen and pre-sowing seeds bacteryzation on the de-
velopment of nitrogen fixing bacteria and the activity of autotrophic and heterotrophic nitrification.
It was revealed that nitrification activity in the root zone of winter rye plants rises together with the
increase of mineral nitrogen doses. Heterotrophic nitrification plays a significant role in the for-
mation of nitrate pool in the root zone, especially during the early stages of plants organogenesis.
Application of the microbial preparation Diazobakteryn had enhanced the activity autotrophic and
heterotrophic nitrification processes in the rhizosphere soil of plants in the spring, during the early
stages of their development while during the next organogenesis phases the reduction of nitrifica-
tion processes was observed. In plants-free soil the contribution of heterotrophic nitrification to
biological transformation of nitrogen was negligible.
Key words: autotrophic nitrification, heterotrophic nitrification, Diazobakteryn, winter rye,
inoculation, fertilizers.
Microbiological transformation of nitrogen
compounds to nitrates plays an important role in
the nitrogen cycle: on the one hand it increases
the denitrification activity (by increasing the
concentration of nitrates in the soil), on the
other — limits the activity of nitrogen fixation
and microbial immobilization of nitrogen [1].
Nitrification is most active if the sufficient amo-
unt of ammonia nitrogen is present under the
soil pH close to neutral and sufficient aeration.
Since these conditions are also favorable for
plants growth and development, the nitrification
rate in soils is often used for diagnostic purpo-
ses [2].
Peculiarities of autotrophic and hetero-
trophic nitrification depend upon the type of
biogeocenosis [3]. In soils of natural ecosystems
(e. g., forest cenosis) heterotrophic nitrification
is dominant, which mainly occurred due to the
fungal activity [4; 5]. In agrocenosis of cultiva-
ted soils for contrast, the share of autotrophic
nitrifying microorganisms account for almost
84–99 % of ammonium oxidation [3], as the soil
cultivation creates favorable conditions for the
operation nitrification autotrophic bacteria: no
climax plant groups are present, crop rotation,
enhanced mineralization of nitrogen compo-
unds, increased soil aeration; use of manure, and
ammonium fertilizers, served as an energy sub-
strate for nitrifying autotrophic bacteria. At the
same time, the reduction of fungi population,
many of which are capable of heterotrophic ni-
trification, was observed in the arable soils. All
this leads to almost complete transfer of leading
position to nitrifying autotrophic bacteria.
Kurakov A. V. with his colleagues [6] have
found that in laboratory conditions heterotrophic
microorganisms contribute to the formation of
nitrates in virgin soils in average of 70 %, while
the share of nitrifying autotrophic bacteria is on-
ly 30 %. In cultivated soils, 73–92 % of nitrates
are formed by autotrophic nitrifying bacteria.
Among the issues related to the nitrification
processes, the behavior of rhizosphere microor-
ganisms towards the ammonium oxidation re-
mains unexplored. Theoretically, within given
ecological niche heterotrophs should prevail,
due to the presence of carbon in root exudates,
but plants can also influence the nitrate assimi-
lation. Investigation of ammonium oxidation pe-
© I. G. Korotka, V. V. Volkogon, 2015
45
culiarities in the root zone of plants under the
inoculation and application of mineral fertilizers
can be used as the grounds for optimization ni-
trogen fertilization of agricultural crops.
Materials and methods. The nitrification
process orientation in the root zone of winter
rye plants was investigated under the different
agricultural backgrounds and pre-sowing seed
inoculation with microbial preparation Diazo-
bacteryn (agent Azospirillum brasilense 18-2)
(TU 24.1-00497360-002:2005) in a small plots
field experiments on sod-podzolic silty-sandy
cultivated soil on experimental field of the Insti-
tute of Agricultural Microbiology and Agroin-
dustrial Manufacture NAAS (pHsol — 6.2, hu-
mus content — 1.2 %, nitrogen content easily
hydrolyzed (by Kornfield) — 54.9 mg/kg,
P2O5 — 330 mg/kg, K2O — 148 mg/kg). Close
to neutral soil pH is caused by the soil cultiva-
tion and liming.
Experimental design:
I. Without bacterization
1. Without fertilizers (control)
2. N30K20 (N10 in fall + N20 early spring)
3. N60K40 (N30 in fall + N30 early spring)
4. N90K60 (N30 in fall + N30 early spring +
+ N30 at stem elongation stage)
5. N120K80 (N30 in fall + N45 early spring +
+ N45 at stem elongation stage)
ІІ. Diazobacteryn bacterization
6. Without fertilizers
7. N30K20 (N10 in fall + N20 early spring)
8. N60K40 (N30 in fall + N30 early spring)
9. N90K60 (N30 in fall + N30 early spring +
+ N30 at stem elongation stage)
10. N120K80 (N30 in fall + N45 early spring +
+ N45 at stem elongation stage)
Calculation of NPK rates was performed
taking into the account the nutritional contents
in the soil and its removal with the planned
yield of 3.5 t/ha. The size of experimental plots
was 9 sq. m. In order to exclude the plants role
in the nitrification processes the fallow plots
(1 m × 1 m) were used.
The number of autotrophic nitrifying bacte-
ria of phase I was determined by gradual dilu-
tion in Vinogradsky liquid medium. The number
of autotrophic nitrifying bacteria of phase II —
on the Watson-Waterbern culture medium [7].
The number of cells per volume unit was calcu-
lated using McCready table based on variation
statistics methods.
Composting method by D. Zvyagintsev [8]
was used to study the nitrification process and
evaluation of the contribution of autotrophic and
heterotrophic nitrifying microorganisms in the
nitrate formation. To determine the potential ni-
trifying activity some bottles were not only wa-
tered but have received the ammonium sulfate
(100 mg N-NH4 / g soil) and thoroughly mixed.
In order to isolate the heterotrophic component
in nitrate accumulation, the course of auto-
trophic nitrification was inhibited with 4-amino-
1,2,4-triazole (100 mg / g soil). Nitrate contents
was determined using ion-selective electrodes
[9]. The three-fold experimental replication was
used.
Results and discussion. Under the favora-
ble conditions large portion of nitrogen fertili-
zers are subject to nitrification. According to
A. I. Nesterova [10] in well aerated slightly acid
soils with high biological activity more than half
of the introduced ammonium is nitrified in
15 days. The studies of the influence of mineral
nitrogen and seeds inoculation with Diazobacte-
ryn on the number of nitrifying autotrophic bac-
teria in rhizosphere soil of winter rye plants
have revealed that the introduction of nitrogen
fertilizers had contributed to the increase of au-
totrophic nitrifying bacteria. Seeds inoculation
with Diazobacteryn had no clear patterns re-
garding the influence of biological preparation
on the development of nitrifying bacteria in
2011 (Table 1). However, the studies performed
in 2012 and 2013 have indicated its positive im-
pact on the development of nitrifying bacteria
(Tables 2–3).
Thus, Diazobacteryn use can provide a
comfortable environment for plants growth and
development by contributing to the increase of
the number nitrifying bacteria and their activity
due to their better nutrition supply with nitrates.
Inhibitory approach in determining the ni-
trification activity can provide a differentiated
evaluation of auto- and heterotrophic nitrifying
microorganisms’ part in oxidative link of nitro-
gen cycle.
Determination of the total nitrification ac-
tivity in 2013 had showed an process activity
increase according to the introduced doses of ni-
trogen fertilizers. The highest nitrification acti-
vity was observed at the beginning of the gro-
wing season (Fig. 1), with the subsequent reduc-
tion during the plant ontogenesis (Fig. 2–3).
The studies performed have indicated that
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2015. — Вип. 21.
46
Table 1. Influence of seeds inoculation and mineral fertilizers on the development of nitri-
fying bacteria in rhizosphere soil of winter rye plants, 2011
Variants
Number of bacteria, tsnd. in 1 g dry soil
Autotrophic nitrifying bacteria
of I phase
Autotrophic nitrifying bacteria
of II phase
Stem
elongation Flowering Milk stage Stem
elongation Flowering Milk stage
Without inoculation
Without fertili-
zers, control 0,5 1,0 0,5 0,3 0,3 0,3
N30K20 2,6 1,0 0,3 0,3 0,3 0,5
N60K40 9,9 2,6 0,3 0,3 0,3 0,9
N90K60 10,0 4,7 2,9 0,5 2,6 28,7
N120K80 9,9 7,7 5,2 2,1 4,6 51,6
Inoculation with Diazobacteryn
Without
fertilizers 0,3 0,5 0,3 0,3 0,3 0,5
N30K20 1,0 0,5 0,3 0,3 0,3 1,1
N60K40 4,7 1,0 0,3 0,3 0,5 5,2
N90K60 7,9 2,6 1,7 1,6 2,6 5,2
N120K80 10,0 9,8 1,1 2,6 4,6 8,6
Notes: * — determination on Vinogradskiy medium; ** — determination on Watson-Waterbern medium.
Table 2. Influence of seeds inoculation and mineral fertilizers on the development of nitri-
fying bacteria in rhizosphere soil of winter rye plants, 2012
Variants
Number of bacteria, tsnd. in 1 g dry soil
Autotrophic nitrifying bacteria
of I phase
Autotrophic nitrifying bacteria
of II phase
Stem
elongation Flowering Milk stage Stem
elongation Flowering Milk stage
Without inoculation
Without fertili-
zers, control 4,8 7,9 2,1 2,6 1,0 0,5
N30K20 4,8 9,9 2,1 2,7 1,0 2,6
N60K40 26,7 9,9 10,1 2,7 1,0 2,7
N90K60 26,7 15,6 26,1 4,8 2,6 9,9
N120K80 26,2 20,8 26,0 4,7 4,7 9,9
Inoculation with Diazobacteryn
Without
fertilizers 8,0 10,0 1,6 2,7 1,0 1,0
N30K20 10,1 12,2 8,50 2,7 2,7 1,0
N60K40 10,2 15,8 48,4 2,7 2,6 2,7
N90K60 79,1 15,7 47,8 10,0 4,7 2,7
N120K80 78,3 26,1 100,6 15,7 9,9 4,8
Notes: * — determination on Vinogradskiy medium; ** — determination on Watson-Waterbern medium.
the activity increase at the beginning of the gro-
wing season (tillering stage) was caused by the
heterotrophic nitrification. Further contribution
of heterotrophs to the formation of a pool of ni-
trates was insignificant and was stable during
the growing season. Using the microbial prepa-
ration Diazobacteryn in the technology of win-
ter rye growing had magnified the nitrification
process during the early stages of plants organ-
ogenesis. Comparison of autotrophic and hete-
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2015. — Вип. 21.
47
Table 3. Influence of seeds inoculation and mineral fertilizers on the development of nitri-
fying bacteria in rhizosphere soil of winter rye plants, 2013
Variants
Number of bacteria, tsnd. in 1 g dry soil
Autotrophic nitrifying bacteria
of I phase
Autotrophic nitrifying bacteria
of II phase
Stem
elongation Flowering Milk stage Stem
elongation Flowering Milk stage
Without inoculation
Without fertili-
zers, control 5,3 2,6 2,8 3,0 1,0 1,7
N30K20 5,4 2,7 2,9 3,0 1,6 2,9
N60K40 29,9 2,6 5,3 3,0 2,1 2,9
N90K60 29,6 4,7 8,6 5,3 2,6 5,2
N120K80 29,6 7,9 11,1 5,3 4,7 5,2
Inoculation with Diazobacteryn
Without
fertilizers 8,9 2,6 2,9 3,0 1,0 1,1
N30K20 8,9 2,6 3,0 3,0 2,6 1,8
N60K40 11,3 4,6 2,9 3,0 2,6 2,9
N90K60 55,1 9,8 5,3 9,2 4,6 3,0
N120K80 54,5 26,2 11,4 9,1 10,0 5,4
Notes: * — determination on Vinogradskiy medium; ** — determination on Watson-Waterbern medium.
Fig. 1. Nitrification activity in rhizosphere soil of winter rye plants under the influence of mineral
fertilizers and inoculation, 2013; Tillering stage (in 14 days after spring application of planned
dose of mineral fertilizers); µg N-No3 / g soil.
Notes: LSD05 for total nitrification activity: for experiment — 6.07, for fertilizers — 4.29, for inoculation
and interaction — 2.48; LSD05 for autotrophic nitrification activity: for experiment — 0.82, for fertili-
zers — 0.58, for inoculation and interaction — 0.34; LSD05 for heterotrophic nitrification activity: for ex-
periment — 5.79, for fertilizers — 4.09, for inoculation and interaction — 2.36.
N
itr
ifi
ca
tio
n
ac
tiv
ity
,
µg
N
-N
O
3/g
so
il
Without bacterization:
1 — without fertilizers
2 — N30K20
3 — N60K40
4 — N90K60
5 — N120K80
Inoculatin with
Diazobacterin:
6 — without fertilizers
7 — N30K20
8 — N60K40
9 — N90K60
10 — N120K80
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2015. — Вип. 21.
48
Fig. 2. Nitrification activity in rhizosphere soil of winter rye plants under the influence of mine-
ral fertilizers and inoculation, 2013; Flowering stage (in 50 days after the early spring fertili-
zers application and 13 days after the plants dressing with mineral nitrogen in corresponded
variants); µg N-NO3 / g soil.
Notes: LSD05 for total nitrification activity: for experiment — 3.90, for fertilizers — 2.76, for inoculation
and interaction — 1.59; LSD05 for autotrophic nitrification activity: for experiment — 0.76, for fertiliz-
ers — 0.54, for inoculation and interaction — 0.31; LSD05 for heterotrophic nitrification activity: for
experiment — 0.99, for fertilizers — 0.70, for inoculation and interaction — 0.40.
Fig. 3. Nitrification activity in rhizosphere soil of winter rye plants under the influence of mi-
neral fertilizers and inoculation, 2013; Milk stage; µg N-NO3 / g soil.
Notes: LSD05 for total nitrification activity: for experiment — 1.62, for fertilizers — 1.14, for inoculation
and interaction — 0.66; LSD05 for autotrophic nitrification activity: for experiment — 1.97, for fertili-
zers — 1.39, for inoculation and interaction — 0.80; LSD05 for heterotrophic nitrification activity: for
experiment — 0.54, for fertilizers — 0.38, for inoculation and interaction — 0.22.
Without bacterization:
1 — without fertilizers
2 — N30K20
3 — N60K40
4 — N90K60
5 — N120K80
Inoculatin with
Diazobacterin:
6 — without fertilizers
7 — N30K20
8 — N60K40
9 — N90K60
10 — N120K80
Without bacterization:
1 — without fertilizers
2 — N30K20
3 — N60K40
4 — N90K60
5 — N120K80
Inoculatin with
Diazobacterin:
6 — without fertilizers
7 — N30K20
8 — N60K40
9 — N90K60
10 — N120K80
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2015. — Вип. 21.
49
rotrophic nitrification indicators had indicated
that the observed increase was caused by the in-
tensification of heterotrophic microorganisms.
Later, with the development of plants, its activi-
ty in the variants with bacterization have re-
duced, probably due to the intense plant growth
and, consequently, with a decrease of ammoni-
um content in soil.
As for almost a century (since the discovery
of autotrophic nitrification process by S. M. Vi-
nogradskiy) it was believed that nitrification is
performed only by autotrophic nitrifying bacte-
ria of the first and second phases and only re-
cently the existence of heterotrophic nitrifica-
tion process was established. This naturally
raises the question whether for a prolonged time
the statement on the exclusive role of auto-
trophic nitrification was based on outdated
methods or the whole process was studied ex-
clusively in topsoil without taking into the ac-
count the plants role in the process of nitrogen
transformation. In our studies we’ve tried to
clarify this.
In order to exclude role of plants in the ni-
trification process we have determined the po-
tential autotrophic and heterotrophic nitrifica-
tion activity on fallow fields and have compared
data with the one obtained from the rhizosphere
soil. As noted above, plants play the main role
in the intensification of nitrification process at
the beginning of the growing season, providing
carbon compounds to the bacteria along with the
soil root exudates (Table 4). Thus in rhizosphere
soil of winter rye plants the total nitrification ac-
tivity was in 1.5–3 times higher comparing to
the activity of the process in the fallow soil
plots.
It should be noted that at the beginning of
vegetation the activity of heterotrophic nitrifica-
tion in the rhizosphere soil of winter rye plants
was in 1.5–5 times higher comparing to the fal-
low soil (Table 5). The potential activity of he-
terotrophic nitrification in the fallow soils was
11.08–12.09 µg N-NO3 / g soil, while the nitri-
fication activity in rhizosphere soil of winter rye
plants had ranged from 17.96 to 70.34 µg
N-NO3 / g soil depending upon the dose of ferti-
lizers.
The obtained results were absolutely logical
as in rhizosphere soil the number of easily ac-
cessible for heterotrophic nitrifying microorga-
nisms organic substances is significantly higher
(i. e., “rhizosphere effect” already known for
other groups of microorganisms). Throughout
the growing season activity heterotrophic nitri-
fication in the rhizosphere soil of winter rye
plants was also higher than in fallow soils.
However, the highest indicators was observed in
during the tillering stage, when the root exuda-
tion is likely the highest.
We should also note the positive impact of
plants on the activity of autotrophic nitrifying
bacteria, which, we assume, is related to the in-
crease of CO2 flow to the root zone of plants
because of metabolic conversion of organic
compounds of root exudates.
Table 4. Total nitrification activity in rhizosphere and fallow soils, field experiment, 2013,
µg N-NO3 / g soil
Variants Tillering stage Flowering stage Milk stage
Without fertilizers
Fallow soil 82,66 ± 5,68 44,65 ± 1,52 43,01 ± 5,69
Rhizosphere soil 302,45 ± 9,45 71,36 ± 7,41 101,20 ± 6,74
N30K20
Fallow soil 108,31 ± 2,19 70,06 ± 5,77 78,99 ± 3,11
Rhizosphere soil 343,53 ± 2,40 92,08 ± 8,82 107,93 ± 7,00
N60K40
Fallow soil 138,35 ± 13,09 87,03 ± 2,34 92,91 ± 1,89
Rhizosphere soil 332,12 ± 6,39 117,97 ± 5,15 118,12 ± 8,77
N90K60
Fallow soil 290,08 ± 6,32 214,65 ± 20,45 152,67 ± 9,12
Rhizosphere soil 362,48 ± 7,62 293,21 ± 27,13 175,62 ± 11,65
N120К80
Fallow soil 297,94 ± 10,99 238,63 ± 8,29 160,09 ± 10,60
Rhizosphere soil 389,56 ± 6,31 319,59 ± 22,93 218,56 ± 5,98
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2015. — Вип. 21.
50
Table 5. Activity of heterotrophic nitrification in rhizosphere and fallow soils, field experi-
ment, 2013, µg N-NO3 / g soil
Variants Tillering stage Flowering stage Milk stage
Without fertilizers
Fallow soil 11,80 ± 1,14 2,17 ± 0,03 1,26 ± 0,16
Rhizosphere soil 17,96 ± 0,59 2,17 ± 0,18 2,40 ± 0,26
N30K20
Fallow soil 11,34 ± 0,56 2,50 ± 0,23 1,80 ± 0,10
Rhizosphere soil 37,80 ± 0,79 2,63 ± 0,18 3,38 ± 0,47
N60K40
Fallow soil 12,09 ± 0,15 2,12 ± 0,41 2,08 ± 0,03
Rhizosphere soil 54,45 ± 0,40 3,25 ± 0,45 3,84 ± 0,34
N90K60
Fallow soil 11,83 ± 0,26 3,56 ± 0,21 3,29 ± 0,39
Rhizosphere soil 60,89 ± 0,82 4,71 ± 0,47 4,88 ± 0,33
N120R80
Fallow soil 12,06 ± 0,17 5,16 ± 0,23 5,17 ± 0,39
Rhizosphere soil 70,34 ± 1,10 7,62 ± 0,55 7,45 ± 0,48
Consequently, the nitrification activity in
the root zone of winter rye plants rises with the
increasing doses of mineral nitrogen. Hetero-
trophic nitrification plays a significant role in
the formation of nitrate pool in the root zone of
plants, especially during the early stages of
plants organogenesis. Pre-sowing bacterization
of winter rye seeds ensures the growth of auto-
trophic and heterotrophic nitrification process
activity in the rhizosphere soil of plants during
the early stages of their development and its re-
duction during the next phases of ontogenesis.
In fallow soils the contribution of heterotrophic
nitrification process into the biological trans-
formation of nitrogen was negligible.
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бьева, Б. А. Бызов и др.] ; под ред. Д. Г. Звягин-
цева. — М. : Изд-во МГУ, 1991. — 304 с.
9. Почвы. Определение нитратов иономет-
рическим методом. ГОСТ 26951-86. Введ.
01.07.1987. — М. : Изд-во стандартов, 1986. —
10 с.
10. Агрономическая микробиология / под
ред. Г. С. Муромцева. — Л. : Колос, 1976. —
231 с.
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2015. — Вип. 21.
51
ОСОБЛИВОСТІ ПРОЦЕСІВ
АВТОТРОФНОЇ І ГЕТЕРОТРОФНОЇ
НІТРИФІКАЦІЇ В КОРЕНЕВІЙ ЗОНІ
РОСЛИН ЖИТА ОЗИМОГО
ЗА ВПЛИВУ МІНЕРАЛЬНИХ ДОБРИВ
ТА ДІАЗОБАКТЕРИНУ
І. Г. Коротка, В. В. Волкогон
Інститут сільськогосподарської мікробіології
та агропромислового виробництва НААН,
м. Чернігів
Досліджено вплив мінерального азоту
та передпосівної бактеризації на розвиток
нітрифікувальних мікроорганізмів та актив-
ність процесів автотрофної і гетеротроф-
ної нітрифікації. Активність нітрифікації в
кореневій зоні рослин жита озимого зростає
при збільшенні доз мінерального азоту. Ге-
теротрофна нітрифікація відіграє помітну
роль у формуванні нітратного пулу в корене-
вій зоні рослин, особливо на початкових
етапах органогенезу. Застосування мікроб-
ного препарату Діазобактерину забезпечує
зростання активності процесів автотроф-
ної і гетеротрофної нітрифікації в ризо-
сферному ґрунті рослин навесні, на перших
етапах їх розвитку, і зменшення — у подаль-
ші фази органогенезу. У ґрунті без рослин
внесок гетеротрофної нітрифікації в проце-
си біологічної трансформації азоту незнач-
ний.
Ключові слова: автотрофна нітрифіка-
ція, гетеротрофна нітрифікація, Діазобак-
терин, жито озиме, інокуляція, мінеральні
добрива.
ОСОБЕННОСТИ ПРОЦЕССОВ АВТО-
ТРОФНОЙ И ГЕТЕРОТРОФНОЙ
НИТРИФИКАЦИИ В КОРНЕВОЙ ЗОНЕ
РАСТЕНИЙ ОЗИМОЙ РЖИ ПОД
ВЛИЯНИЕМ МИНЕРАЛЬНЫХ
УДОБРЕНИЙ И ДИАЗОБАКТЕРИНА
И. Г. Короткая, В. В. Волкогон
Институт сельскохозяйственной микробиологии
и агропромышленного производства НААН,
г. Чернигов
Исследовано влияние минерального азо-
та и предпосевной бактеризации на разви-
тие нитрифицирующих микроорганизмов и
активность процессов автотрофной и ге-
теротрофной нитрификации. Активность
нитрификации в корневой зоне растений
ржи озимой возрастает при увеличении доз
минерального азота. Гетеротрофная нит-
рификация играет заметную роль в форми-
ровании нитратного пула в корневой зоне
растений, особенно на начальных этапах ор-
ганогенеза. Применение микробного препа-
рата Диазобактерина обеспечивает усиле-
ние активности процессов автотрофной и
гетеротрофной нитрификации в ризосфер-
ной почве растений весной, на первых эта-
пах их развития, и уменьшение — в после-
дующие фазы органогенеза. В почве без рас-
тений вклад гетеротрофной нитрификации
в процессы биологической трансформации
азота незначителен.
Ключевые слова: автотрофная нитри-
фикация, гетеротрофная нитрификация,
Диазобактерин, рожь озимая, инокуляция,
минеральные удобрения.
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2015. — Вип. 21.
|
| id | oai:ojs2.smic.in.ua:article-145 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2026-07-23T01:04:37Z |
| publishDate | 2015 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/81/89b6fcc8a3c062410c946d00ac3bcf81.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1452026-07-22T10:10:43Z THE FEATURES THE AUTOTROPHIC AND HETEROTROPHIC NITRIFICATION IN THE ROOT ZONE OF WINTER RYE PLANTS UNDER THE USE OF MINERAL FERTILIZERS AND DIAZOBAKTERYN ОСОБЛИВОСТІ ПРОЦЕСІВ АВТОТРОФНОЇ І ГЕТЕРОТРОФНОЇ НІТРИФІКАЦІЇ В КОРЕНЕВІЙ ЗОНІ РОСЛИН ЖИТА ОЗИМОГО ЗА ВПЛИВУ МІНЕРАЛЬНИХ ДОБРИВ ТА ДІАЗОБАКТЕРИНУ Коротка, І. Г. Волкогон, В. В. autotrophic nitrification, heterotrophic nitrification, Diazobakteryn, winter rye, inoculation, fertilizers автотрофна нітрифікація, гетеротрофна нітрифікація, Діазобактерин, жито озиме, інокуляція, мінеральні добрива The paper depicts the study of mineral nitrogen and pre-sowing seeds bacteryzation on the development of nitrogen fixing bacteria and the activity of autotrophic and heterotrophic nitrification. It was revealed that nitrification activity in the root zone of winter rye plants rises together with the increase of mineral nitrogen doses. Heterotrophic nitrification plays a significant role in the formation of nitrate pool in the root zone, especially during the early stages of plants organogenesis. Application of the microbial preparation Diazobakteryn had enhanced the activity autotrophic and heterotrophic nitrification processes in the rhizosphere soil of plants in the spring, during the early stages of their development while during the next organogenesis phases the reduction of nitrification processes was observed. In plants-free soil the contribution of heterotrophic nitrification to biological transformation of nitrogen was negligible. Досліджено вплив мінерального азоту та передпосівної бактеризації на розвиток нітрифікувальних мікроорганізмів та активність процесів автотрофної і гетеротрофної нітрифікації. Активність нітрифікації в кореневій зоні рослин жита озимого зростає при збільшенні доз мінерального азоту. Гетеротрофна нітрифікація відіграє помітну роль у формуванні нітратного пулу в кореневій зоні рослин, особливо на початкових етапах органогенезу. Застосування мікробного препарату Діазобактерину забезпечує зростання активності процесів автотрофної і гетеротрофної нітрифікації в ризосферному ґрунті рослин навесні, на перших етапах їх розвитку, і зменшення — у подальші фази органогенезу. У ґрунті без рослин внесок гетеротрофної нітрифікації в процеси біологічної трансформації азоту незначний. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2015-08-06 Article Article Рецензована Стаття application/pdf https://smic.in.ua/index.php/journal/article/view/145 10.35868/1997-3004.21.44-51 Agricultural microbiology; Vol. 21 (2015): Agriciltural microbiology; 44-51 Сільськогосподарська мікробіологія; Том 21 (2015): Сільськогосподарська мікробіологія; 44-51 1997-3004 10.35868/1997-3004.21 en https://smic.in.ua/index.php/journal/article/view/145/176 Авторське право (c) 2015 I. G. Korotka, V. V. Volkogon https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | автотрофна нітрифікація гетеротрофна нітрифікація Діазобактерин жито озиме інокуляція мінеральні добрива Коротка, І. Г. Волкогон, В. В. ОСОБЛИВОСТІ ПРОЦЕСІВ АВТОТРОФНОЇ І ГЕТЕРОТРОФНОЇ НІТРИФІКАЦІЇ В КОРЕНЕВІЙ ЗОНІ РОСЛИН ЖИТА ОЗИМОГО ЗА ВПЛИВУ МІНЕРАЛЬНИХ ДОБРИВ ТА ДІАЗОБАКТЕРИНУ |
| title | ОСОБЛИВОСТІ ПРОЦЕСІВ АВТОТРОФНОЇ І ГЕТЕРОТРОФНОЇ НІТРИФІКАЦІЇ В КОРЕНЕВІЙ ЗОНІ РОСЛИН ЖИТА ОЗИМОГО ЗА ВПЛИВУ МІНЕРАЛЬНИХ ДОБРИВ ТА ДІАЗОБАКТЕРИНУ |
| title_alt | THE FEATURES THE AUTOTROPHIC AND HETEROTROPHIC NITRIFICATION IN THE ROOT ZONE OF WINTER RYE PLANTS UNDER THE USE OF MINERAL FERTILIZERS AND DIAZOBAKTERYN |
| title_full | ОСОБЛИВОСТІ ПРОЦЕСІВ АВТОТРОФНОЇ І ГЕТЕРОТРОФНОЇ НІТРИФІКАЦІЇ В КОРЕНЕВІЙ ЗОНІ РОСЛИН ЖИТА ОЗИМОГО ЗА ВПЛИВУ МІНЕРАЛЬНИХ ДОБРИВ ТА ДІАЗОБАКТЕРИНУ |
| title_fullStr | ОСОБЛИВОСТІ ПРОЦЕСІВ АВТОТРОФНОЇ І ГЕТЕРОТРОФНОЇ НІТРИФІКАЦІЇ В КОРЕНЕВІЙ ЗОНІ РОСЛИН ЖИТА ОЗИМОГО ЗА ВПЛИВУ МІНЕРАЛЬНИХ ДОБРИВ ТА ДІАЗОБАКТЕРИНУ |
| title_full_unstemmed | ОСОБЛИВОСТІ ПРОЦЕСІВ АВТОТРОФНОЇ І ГЕТЕРОТРОФНОЇ НІТРИФІКАЦІЇ В КОРЕНЕВІЙ ЗОНІ РОСЛИН ЖИТА ОЗИМОГО ЗА ВПЛИВУ МІНЕРАЛЬНИХ ДОБРИВ ТА ДІАЗОБАКТЕРИНУ |
| title_short | ОСОБЛИВОСТІ ПРОЦЕСІВ АВТОТРОФНОЇ І ГЕТЕРОТРОФНОЇ НІТРИФІКАЦІЇ В КОРЕНЕВІЙ ЗОНІ РОСЛИН ЖИТА ОЗИМОГО ЗА ВПЛИВУ МІНЕРАЛЬНИХ ДОБРИВ ТА ДІАЗОБАКТЕРИНУ |
| title_sort | особливості процесів автотрофної і гетеротрофної нітрифікації в кореневій зоні рослин жита озимого за впливу мінеральних добрив та діазобактерину |
| topic | автотрофна нітрифікація гетеротрофна нітрифікація Діазобактерин жито озиме інокуляція мінеральні добрива |
| topic_facet | autotrophic nitrification heterotrophic nitrification Diazobakteryn winter rye inoculation fertilizers автотрофна нітрифікація гетеротрофна нітрифікація Діазобактерин жито озиме інокуляція мінеральні добрива |
| url | https://smic.in.ua/index.php/journal/article/view/145 |
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