БАКТЕРІАЛЬНА ТРАНСЛОКАЦІЯ ЛАКТОБАЦИЛ-ПРОБІОТИКІВ
The paper presents the results of studying of the dynamics and characteristics of the translocation of two probiotic strains of lactic acid bacteria in the blood and parenchymal organs of animals. The dependence of the bacterial translocation from the bacterial strain and from orally entered dose wa...
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| Date: | 2015 |
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| Format: | Article |
| Language: | English Ukrainian |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2015
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| Online Access: | https://smic.in.ua/index.php/journal/article/view/148 |
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| Journal Title: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465606779240448 |
|---|---|
| author | Агеєв, В. О. |
| author_facet | Агеєв, В. О. |
| author_institution_txt_mv | [
{
"author": "В. О. Агеєв",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Агеєв, В. О. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:43Z |
| description | The paper presents the results of studying of the dynamics and characteristics of the translocation of two probiotic strains of lactic acid bacteria in the blood and parenchymal organs of animals. The dependence of the bacterial translocation from the bacterial strain and from orally entered dose was established. It was shown that cultural-morphological and physiological-biochemical properties of all isolated from blood cultures of microorganisms have corresponded to the initial properties of bacterial strains introduced to the animals. |
| doi_str_mv | 10.35868/1997-3004.21.66-71 |
| first_indexed | 2025-07-17T12:24:06Z |
| format | Article |
| fulltext |
BACTERIAL TRANSLOCATION OF PROBIOTIC LACTOBACILLI
V. O. Aheyev
The effectiveness of probiotic preparations
based on lactic acid bacteria is caused by the
ability of the latter to colonize the inner surface
of the digestive tract and produce a number of
biologically active metabolites (organic acids,
primarily lactic, lysozymes, etc.), which not
only helps protect the body against pathogens,
but also activates its own protective and
adaptive reaction. At the end of the twentieth
century there appeared more experimental data
that contradicts the traditional idea of the
mechanisms of impact of probiotics on a
microorganism. The authors from different
countries pointed out the cases of efficacy of
probiotic preparations that are not subject to
explanation in terms of conventional vision –
not only to treat infections of the digestive tract
in the conditions of the intestine colonization,
but also for the treatment of pathological
processes localized outside the gastrointestinal
tract [1-4].
In this regard, of particular importance is
the study of the phenomenon of translocation of
probiotic strains of microorganisms of different
taxonomic groups (bacilli, Escherichia coli,
bifidobacteria, lactobacilli, etc.), which are the
basis of veterinary and medical preparations of
different functional orientation (preventive,
therapeutic, stimulating), its dynamics,
clarifying the response of the microorganism on
indicators of morphological changes in organs
and its main systems activity. This allows
significantly expand the understanding of the
complex process of interaction of the probiotics
with a macroorganism.
Today it is urgent to develop new
approaches to the strategy and tactics for the
development and use of bacterial biological
products, including probiotics. And thanks to a
comprehensive study of mutual influence of
probiotic bacteria and macroorganism it
becomes possible to create probiotic
preparations intended for the prevention and
treatment of disorders not only of the digestive
system, but many diseases of farm animals and
humans that are located outside the
gastrointestinal tract [5-7] .
One of the mechanisms of such an action of
probiotics is their stimulation of nonspecific
resistance of macroorganism as a result of
translocation of bacteria not only of the upper
gastrointestinal tract (oropharynx and stomach)
- similar processes also continue during further
passage of microorganisms through all parts of
the gastrointestinal tract. In addition,
biologically active compounds produced by
cells of the intestine in response to interactions
with bacteria and are produced directly by
microorganisms make an important effect on
the stimulation of the immune system, by being
soaked in blood and lymph [8].
Given the above, the objective of the work
was to determine the dependence of bacterial
translocation on the strain of probiotic lactic
acid bacteria.
Materials and methods. The strains of
lactic acid bacteria Lactobacillus plantarum L5
(part of the preparation БПС-Л) [9] and L.
plantarum Lc-18 from the collection of
probiotics laboratory, promising to create a new
bacterial drug on its basis, were used for the
research. Growing the cultures of lactic acid
bacteria was carried out on de Man nutrient
medium (MRS) [10]. Growing test cultures to
determine the antagonistic activity was carried
out on the surface of meat-and-peptone agar
(MPA) [11].
Before the study of bacterial translocation
cultural-morphological (the morphology of
cells and colonies, especially the growth on
nutrient media) and physiological and
biochemical (the activity of ectoenzymes, the
ability to ferment various sources of carbon,
antagonistic activity) properties of the original
probiotic cultures were determined, that
distinguish them from other lactic acid bacteria -
representatives of normal microflora of animals
[11-14].
To study the translocation dynamics of
investigated bacteria in the blood of animals
there were formed 5 groups of laboratory rabbits
numbered 3 heads each – one control and four
experimental; control animals were intact
(received the appropriate amount of saline),
animals of experimental group-1 were given the
culture of the bacteria strain Lactobacillus
plantarum L5 at the dose of 3*108 CFU,
animals of experimental group-2 – the same
strain at the dose of 3*109 CFU, experimental
group-3 – the culture of L. plantarum Lc-18 at
the dose of 3*108 CFU, experimental group-4 –
the same culture at the dose of 3*109 CFU. The
suspensions of bacterial cells in saline were
injected to animals once orally. Within 3, 10,
30, 60 min., 24 hr., 7 and 14 days after the
administration of bacteria in each animal blood
samples were taken.
To study the translocation dynamics of
investigated bacteria in parenchymal organs
(liver, spleen, kidneys) of laboratory animals
there were formed 5 groups of white mice
numbered 15 heads each – one control and four
experimental; control animals were intact
(received the appropriate amount of saline),
animals of experimental group-1 were given the
culture of the bacteria strain L. plantarum L5 at
the dose of 3*107 CFU, animals of experimental
group-2 – the same strain at the dose of 3*108
CFU, experimental group-3 – a promising
culture of L. plantarum Lc-18 at the dose of
3*107 CFU, experimental group-4 – the same
culture at the dose of 3*108 CFU. The
suspensions of bacterial cells in saline were
injected to animals once orally. After 30, 60
min., 24 hr., 7 and 14 days 3 animals from each
group were taken out of the experiment, the
samples of their liver, spleen, kidneys, and
blood were taken [3; 5].
Blood was mixed with a defined part of
anticoagulant during the selection;
homogenates on the saline were received from
parenchymal organs in the ratio of 1:9 (dilution
10-1). To determine the presence and quantity of
bacteria that translocated to the blood and
parenchymal organs of animals, sowings of
tenfold dilution of obtained samples in test tubes
with MRS medium were done [10]. Accounting
was made according to Mc Credit tables [15],
making the recount on the addition of
anticoagulant for blood samples.
Housing, feeding, care and all
manipulations with laboratory animals were
carried out under the European Convention “For
the protection of vertebrate animals used for
experimental and other scientific purposes”
(Strasbourg, 1986) [16] and “General ethical
principles of experiments on animals” approved
at the first National Congress on Bioethics
(Kyiv, 2001) [17]. Experiments were conducted
in compliance with the principles of humanity
set out in the European Community Directive
[18].
Mathematical processing of the results,
which are quantified, was made by the methods
of variation statistics using Student t-test and
correlation coefficients [19].
So, a crucial dependence of bacterial
translocation manifestation from introduced
strain of bacteria was established. Thus, the
bacterial strain Lactobacillus plantarum L5 is
characterized by weak capacity for translocation
into the blood and parenchymal organs of a
macroorganism, while the other investigated
microorganism translocation (L. plantarum
Lc-18) is intense.
The dependence of the dynamics of lactic
acid bacteria translocation from the dose was
established: the introduction of bacteria, which
are used to check the safety of probiotics (3*108
CFU) is accompanied by intense translocation,
beginning in the first 10 minutes after the
introduction and the concentration of cells in the
body remains stable for a quite long time (7
days); the introduction of a lower dose of
bacteria (3*107 CFU) causes a smoother start of
a bacterial translocation and significantly lower
concentrations of bacteria in the blood and
tissues of parenchymal organs.
|
| id | oai:ojs2.smic.in.ua:article-148 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:04:43Z |
| publishDate | 2015 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/c9/63716d09630f6bb90149f40cd6791bc9.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1482026-07-22T10:10:43Z BACTERIAL TRANSLOCATION OF PROBIOTIC LACTOBACILLI БАКТЕРІАЛЬНА ТРАНСЛОКАЦІЯ ЛАКТОБАЦИЛ-ПРОБІОТИКІВ Агеєв, В. О. lactic acid bacteria, bacterial translocation, blood, parenchymal organs молочнокислі бактерії, бактеріальна транслокація, кров, паренхіматозні органи The paper presents the results of studying of the dynamics and characteristics of the translocation of two probiotic strains of lactic acid bacteria in the blood and parenchymal organs of animals. The dependence of the bacterial translocation from the bacterial strain and from orally entered dose was established. It was shown that cultural-morphological and physiological-biochemical properties of all isolated from blood cultures of microorganisms have corresponded to the initial properties of bacterial strains introduced to the animals. Представлено результати вивчення динаміки та особливостей транслокації двох пробіотичних штамів молочнокислих бактерій у кров та паренхіматозні органи тварин. Встановлено залежність прояву бактеріальної транслокації від штаму бактерій та від перорально введеної дози. Культурально-морфологічні та фізіолого-біохімічні властивості всіх виділених з крові та органів культур мікроорганізмів відповідають властивостям вихідних бактеріальних штамів, що були введені тваринам. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2015-08-06 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/148 10.35868/1997-3004.21.66-71 Agricultural microbiology; Vol. 21 (2015): Agriciltural microbiology; 66-71 Сільськогосподарська мікробіологія; Том 21 (2015): Сільськогосподарська мікробіологія; 66-71 1997-3004 10.35868/1997-3004.21 en uk https://smic.in.ua/index.php/journal/article/view/148/181 https://smic.in.ua/index.php/journal/article/view/148/182 Авторське право (c) 2015 V. O. Aheyev https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | молочнокислі бактерії бактеріальна транслокація кров паренхіматозні органи Агеєв, В. О. БАКТЕРІАЛЬНА ТРАНСЛОКАЦІЯ ЛАКТОБАЦИЛ-ПРОБІОТИКІВ |
| title | БАКТЕРІАЛЬНА ТРАНСЛОКАЦІЯ ЛАКТОБАЦИЛ-ПРОБІОТИКІВ |
| title_alt | BACTERIAL TRANSLOCATION OF PROBIOTIC LACTOBACILLI |
| title_full | БАКТЕРІАЛЬНА ТРАНСЛОКАЦІЯ ЛАКТОБАЦИЛ-ПРОБІОТИКІВ |
| title_fullStr | БАКТЕРІАЛЬНА ТРАНСЛОКАЦІЯ ЛАКТОБАЦИЛ-ПРОБІОТИКІВ |
| title_full_unstemmed | БАКТЕРІАЛЬНА ТРАНСЛОКАЦІЯ ЛАКТОБАЦИЛ-ПРОБІОТИКІВ |
| title_short | БАКТЕРІАЛЬНА ТРАНСЛОКАЦІЯ ЛАКТОБАЦИЛ-ПРОБІОТИКІВ |
| title_sort | бактеріальна транслокація лактобацил-пробіотиків |
| topic | молочнокислі бактерії бактеріальна транслокація кров паренхіматозні органи |
| topic_facet | lactic acid bacteria bacterial translocation blood parenchymal organs молочнокислі бактерії бактеріальна транслокація кров паренхіматозні органи |
| url | https://smic.in.ua/index.php/journal/article/view/148 |
| work_keys_str_mv | AT ageêvvo bacterialtranslocationofprobioticlactobacilli AT ageêvvo bakteríalʹnatranslokacíâlaktobacilprobíotikív |